Allele Dmel\N^Hsp70Bb.PL

General Information
Symbol	Dmel\N^Hsp70Bb.PL	Species	D. melanogaster
Name	heat shock construct of Lieber	FlyBase ID	FBal0033870
Feature type	allele	Associated gene	Dmel\N

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - deletion
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - deletion (Lieber et al., 1993) in vitro construct - regulatory fusion (Lieber et al., 1993)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Hsp70Bb heatshock promoter sequences drive fused wildtype N genomic and cDNA sequences with all but the last three introns deleted. A genomic N polyA tail has also been added. (Lieber et al., 1992)
Carried in construct	P{hsN} (Lai et al., 2001, Lopez-Schier and St. Johnston, 2002, Lieber et al., 1993, Ge et al., 2004, Matsuno et al., 2009)
Cytology
Phenotypic Data
Phenotypic Class
	memory defective \| heat sensitive (Ge et al., 2004, Matsuno et al., 2009)
Phenotype Manifest In
	S2 cell-line (Ahimou et al., 2004)
Detailed Description
	Statement Reference Heat-shocked N[Hsp70Bb.PL] flies show a greater than 4-fold increase in 1-day memory after single-cycle training. (Matsuno et al., 2009) The surface topology of cells expressing N^Hsp70Bb.PL is different from that of control cells. (Ahimou et al., 2004) Adult flies expressing N^Hsp70Bb.PL (using a 37^oC heat shock) show normal reactivity to electric shock and normal olfactory acuity (to methylcyclohexanol and octanol). Flies in which N^Hsp70Bb.PL is expressed using a 30 minute heat shock show normal learning scores in a Pavlovian learning and memory assay, when trained and tested 3 hours after the heat shock. One day memory is also unaffected in these flies when they are subjected to the normal spaced training procedure (10 training cycles), however, flies that have only one or two spaced training sessions show enhanced one day memory compared to control flies. (Ge et al., 2004) Has no effect on the development of otherwise wild type embryos. (Lopez-Schier and St. Johnston, 2002)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement Reference N^Hsp70Bb.PL has memory defective \| heat sensitive phenotype, suppressible by klg^E226/klg^GS10439 (Matsuno et al., 2009) N^Hsp70Bb.PL has memory defective \| heat sensitive phenotype, suppressible by klg^ruslan (Matsuno et al., 2009)
NOT Suppressor of
Statement Reference N^Hsp70Bb.PL is a non-suppressor of lethal \| neurogenic phenotype \| recessive \| germline clone phenotype of nct^agro (Lopez-Schier and St. Johnston, 2002)
Phenotype Manifest In
Other
Statement Reference N^Hsp70Bb.PL, O-fut1^dsRNA.cAa has S2 cell-line phenotype (Ahimou et al., 2004, Ahimou et al., 2004)
Additional Comments
Genetic Interactions
Statement Reference The enhancement of long-term memory by N[Hsp70Bb.PL] over-expression is suppressed in klg[ruslan] or klg[GS10439]/klg[E226] flies. (Matsuno et al., 2009) Cells co-expressing O-fut1^dsRNA.cAa and N^Hsp70Bb.PL show a detachment force from Dl^hs.PL-expressing cells of almost zero. Cells co-expressing O-fut1^dsRNA.cAa and N^Hsp70Bb.PL do not form cell aggregates when mixed and rotated with Dl^hs.PL-expressing cells. (Ahimou et al., 2004)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

Comments
	Stably expressed in S2 cells, and used in Notch signaling assays. (Ahimou et al., 2004) Transfected into S2 cells and used to assay N signalling dynamics. (Wesley and Mok, 2003) Carried in a plasmid and transfected into S2 cells to study the effect of the Dl and wg proteins on N protein. (Wesley and Saez, 2000) Carried in a plasmid and transfected into S2 cells. (Wesley and Saez, 2000) Carried in a plasmid, transfected into S2 cells and used in biochemical assays. (Wesley, 1999) N minigene encoding full length N protein, driven by a heat shock promoter. (Lieber et al., 1993) Carried in a plasmid and used to generate stable S2 cell lines producing heatshock inducible N protein. (Lieber et al., 1992)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 9 )
Reported As
Symbol Synonym	H/N (Lieber et al., 1992) hsN (Lieber et al., 1993) N (Ahimou et al., 2004) N^hs.PL N^Hsp70Bb.PL NFull (Wesley and Mok, 2003) S2-N (Wesley, 1999) S2-NFull (Wesley and Mok, 2003)
Name Synonym	heat shock construct of Lieber
Secondary FlyBase IDs

References ( 11 )
Research paper	Matsuno et al., 2009, Proc. Natl. Acad. Sci. U.S.A. 106(1): 310--315 The Drosophila cell adhesion molecule Klingon is required for long-term memory formation and is regulated by Notch. [FBrf0206600] Ahimou et al., 2004, J. Cell Biol. 167(6): 1217--1229 The adhesion force of Notch with Delta and the rate of Notch signaling. [FBrf0184061] Ge et al., 2004, Proc. Natl. Acad. Sci. U.S.A. 101(27): 10172--10176 Notch signaling in Drosophila long-term memory formation. [FBrf0179219] Wesley and Mok, 2003, Mol. Cell. Biol. 23(16): 5581--5593 Regulation of Notch signaling by a novel mechanism involving suppressor of hairless stability and carboxyl terminus-truncated Notch. [FBrf0162239] Lopez-Schier and St. Johnston, 2002, Dev. Cell 2(1): 79--89 Drosophila nicastrin is essential for the intramembranous cleavage of Notch. [FBrf0141471] Lai et al., 2001, Dev. Cell 1(6): 783--794 Drosophila neuralized is a ubiquitin ligase that promotes the internalization and degradation of delta. [FBrf0141465] Wesley and Saez, 2000, J. Biol. Chem. 275(13): 9099--9101 Notch responds differently to Delta and Wingless in cultured Drosophila cells. [FBrf0126808] Wesley and Saez, 2000, J. Cell Biol. 149(3): 683--696 Analysis of Notch lacking the carboxyl terminus identified in Drosophila embryos. [FBrf0127388] Wesley, 1999, Mol. Cell. Biol. 19(8): 5743--5758 Notch and wingless regulate expression of cuticle patterning genes. [FBrf0111069] Lieber et al., 1993, Genes Dev. 7(10): 1949--1965 Antineurogenic phenotypes induced by truncated Notch proteins indicate a role in signal transduction and may point to a novel function for Notch in nuclei. [FBrf0064583] Lieber et al., 1992, Neuron 9(21): 847--859 Single amino acid substitutions in EGF-like elements of Notch and Delta modify Drosophila development and affect cell adhesion in vitro. [FBrf0057371]

Allele Dmel\NHsp70Bb.PL

Allele Dmel\N^Hsp70Bb.PL