The class IV dendritic arborization neurons in Pka-C1B3 mutant clones show a significant decrease in the complexity of dendritic arbors in respect to the total dendritic length.
Pka-C1B3/+ flies do not show age-related memory impairment seen in wild type.
Pka-C1B3/+ heterozygotes have dramatically improved memory retention curves compared with wild-type flies. Avoidance of naive flies to the odors odors and electrical shocks used during training is not significantly different between wild-type and Pka-C1B3 heterozygous mutants, indicating that the observed increases in memory are not caused by increased sensitivity to these stimuli. Memory retention curves of Pka-C1B3 /+ heterozygotes indicate that early forms of memory, including memory tested immediately after training (3-min memory) and short forms of memory (1-h memory) are not greatly affected. However, memory at later time points, 3 h and 7 h after training, progressively increases relative to wild-type, such that at 7h, memory is approximately double that of wild-type.
Pka-C1B3/+ flies have improved cold shock-resistant 3-h memory, indicating that anesthesia-resistant memory (ARM) is increased in these flies. Pka-C1B3/+ heterozygote show increased 24-h memory after massed training, indicating increased ARM production.
Compared to wild-types, long-term memory is not altered in Pka-C1B3/+ heterozygotes.
Memory enhancement in Pka-C1B3/+ flies begins between 1 and 3 h after single cycle training and reverts to normal within 4 days after spaced training.
Expression of Pka-C1Scer\UAS.T:Zzzz\FLAG using a mushroom body driver (Scer\GAL4c309) abolishes 24-h memory after massed training in a Pka-C1B3/+ heterozygous background.
High-frequency-stimulation-induced miniature release (HFMR) is eliminated in the neuromuscular junctions of Pka-C1B3 animals, in contrast to controls.
Unlike wild-type males, Pka-C1B3/+ males that have undergone courtship conditioning (kept in the presence of a female for 7 hours) do not spend significantly less time engaged in courtship behaviour when placed with a female 5 days after conditioning than non-conditioned males of the same genotype.
Forskolin does not alter the mSC frequency at the larval neuromuscular junction in mutants.
Heterozygotes show increased sensitivity to ethanol in an inebriometer assay.
Clones induce pattern duplications in adult female internal genitalia and female external terminalia and duplications in the adult male terminalia.
Pka-C1B3/Pka-C1Tw2 females are sterile and contain multinucleate nurse cells in egg chambers beyond stage 5. Discontinuities in the actin ring at the site of the intercellular bridges are seen. Border follicle cells sometimes do not migrate or show incomplete migration in Pka-C1B3/Pka-C1Tw2 females.
Heterozygotes are wild type. Homozygous mutant clones in the anterior compartment of the legs and wings causes pattern abnormalities, duplicated wing with mirror symmetry through the centre of the clone and sometimes supernumerary legs.
Homozygous germline clones generate egg chambers with numerous nurse cell fusions.
Heterozygotes exhibit rhythmic locomotor activity in constant darkness and the average circadian rhythm is slightly shorter than wild type. Heterozygotes had ostensibly normal behaviour.
Hemizygotes are larval lethal. Mature oocytes are smaller than wild type and nurse cells are multinucleate.