FlyBase Allele Report: Dmel\Pka-C1[B3]

General Information

Symbol

Dmel\Pka-C1^B3

Species

D. melanogaster

Name

FlyBase ID

FBal0033955

Feature type

allele

Associated gene

Dmel\Pka-C1

Associated Insertion(s)

Carried in Construct

Also Known As

DC0^B3, DCO^B3, pka^B3, PkaC1^B3

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

amorphic allele - genetic evidence

Mutagen

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Li et al., 1995, Pan and Rubin, 1995, Lane and Kalderon, 1993)

loss of function allele

(Sanchez-Herrero et al., 1996, Lane and Kalderon, 1995)

Mutagen

Progenitor genotype

Cytology

Description

Amino acid replacement: W299term.

(Lane and Kalderon, 1993)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2L:9,696,671..9,696,671 [-]

Nucleotide change:

G9696671A

Reported nucleotide change:

G3019A

Amino acid change:

W299term | Pka-C1-PB; W299term | Pka-C1-PC; W299term | Pka-C1-PD

Reported amino acid change:

W299term

(Lane and Kalderon, 1993)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of alcohol use disorder

CEA

(Moore et al., 1998)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates neurofibromatosis

modeled by Nf1^E2

(Walker et al., 2013)

ameliorates kidney cancer

modeled by Ras85D^V12.UAS

(Zeng et al., 2010)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

aedeagus

(Chen and Baker, 1997)

border follicle cell

(Lane and Kalderon, 1995)

cercus

(Chen and Baker, 1997)

dendrite | somatic clone

(Copf, 2014)

egg chamber | germline clone

(Lane and Kalderon, 1994)

epandrial ventral lobe

(Chen and Baker, 1997)

epandrium

(Chen and Baker, 1997)

epiproct

(Chen and Baker, 1997)

hypogynial tooth

(Chen and Baker, 1997)

hypogynium

(Chen and Baker, 1997)

hypoproct

(Chen and Baker, 1997)

larval multidendritic class IV neuron | somatic clone

(Copf, 2014)

leg anterior compartment

(Pan and Rubin, 1995)

neuromuscular junction

(Yoshihara et al., 2005)

nurse cell

(Lane and Kalderon, 1995, Lane and Kalderon, 1993)

nurse cell | germline clone

(Lane and Kalderon, 1994)

oocyte

(Lane and Kalderon, 1993)

perineurium | embryonic stage

(Li et al., 2021)

spermatheca

(Chen and Baker, 1997)

surstylus

(Chen and Baker, 1997)

wing

(Ohlmeyer and Kalderon, 1998)

wing blade anterior compartment

(Pan and Rubin, 1995)

Detailed Description

Statement

Reference

The class IV dendritic arborization neurons in Pka-C1^B3 mutant clones show a significant decrease in the complexity of dendritic arbors in respect to the total dendritic length.

(Copf, 2014)

Pka-C1^B3/+ flies do not show age-related memory impairment seen in wild type.

(Yamazaki et al., 2014)

Pka-C1^B3/+ heterozygotes have dramatically improved memory retention curves compared with wild-type flies. Avoidance of naive flies to the odors odors and electrical shocks used during training is not significantly different between wild-type and Pka-C1^B3 heterozygous mutants, indicating that the observed increases in memory are not caused by increased sensitivity to these stimuli. Memory retention curves of Pka-C1^B3 /+ heterozygotes indicate that early forms of memory, including memory tested immediately after training (3-min memory) and short forms of memory (1-h memory) are not greatly affected. However, memory at later time points, 3 h and 7 h after training, progressively increases relative to wild-type, such that at 7h, memory is approximately double that of wild-type.

Pka-C1^B3/+ flies have improved cold shock-resistant 3-h memory, indicating that anesthesia-resistant memory (ARM) is increased in these flies. Pka-C1^B3/+ heterozygote show increased 24-h memory after massed training, indicating increased ARM production.

Compared to wild-types, long-term memory is not altered in Pka-C1^B3/+ heterozygotes.

Memory enhancement in Pka-C1^B3/+ flies begins between 1 and 3 h after single cycle training and reverts to normal within 4 days after spaced training.

Expression of Pka-C1^{Scer\UAS.T:Zzzz\FLAG} using a mushroom body driver (Scer\GAL4^c309) abolishes 24-h memory after massed training in a Pka-C1^B3/+ heterozygous background.

(Horiuchi et al., 2008)

High-frequency-stimulation-induced miniature release (HFMR) is eliminated in the neuromuscular junctions of Pka-C1^B3 animals, in contrast to controls.

(Yoshihara et al., 2005)

Unlike wild-type males, Pka-C1^B3/+ males that have undergone courtship conditioning (kept in the presence of a female for 7 hours) do not spend significantly less time engaged in courtship behaviour when placed with a female 5 days after conditioning than non-conditioned males of the same genotype.

(Sakai et al., 2004)

Forskolin does not alter the mSC frequency at the larval neuromuscular junction in mutants.

(Yoshihara et al., 2000)

Heterozygotes show increased sensitivity to ethanol in an inebriometer assay.

(Moore et al., 1998)

Clones induce pattern duplications in adult female internal genitalia and female external terminalia and duplications in the adult male terminalia.

(Chen and Baker, 1997)

Pka-C1^B3/Pka-C1^Tw2 transheterozygotes are phenotypically similar to Nf1 mutants, small body size.

(The et al., 1997)

Pka-C1^B3/Pka-C1^Tw2 females are sterile and contain multinucleate nurse cells in egg chambers beyond stage 5. Discontinuities in the actin ring at the site of the intercellular bridges are seen. Border follicle cells sometimes do not migrate or show incomplete migration in Pka-C1^B3/Pka-C1^Tw2 females.

(Lane and Kalderon, 1995)

Heterozygotes are wild type. Homozygous mutant clones in the anterior compartment of the legs and wings causes pattern abnormalities, duplicated wing with mirror symmetry through the centre of the clone and sometimes supernumerary legs.

(Pan and Rubin, 1995)

Homozygous germline clones generate egg chambers with numerous nurse cell fusions.

(Lane and Kalderon, 1994)

Heterozygotes exhibit rhythmic locomotor activity in constant darkness and the average circadian rhythm is slightly shorter than wild type. Heterozygotes had ostensibly normal behaviour.

(Levine et al., 1994)

Hemizygotes are larval lethal. Mature oocytes are smaller than wild type and nurse cells are multinucleate.

(Lane and Kalderon, 1993)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Pka-C1^B3 has abnormal memory | adult stage phenotype, suppressible by Scer\GAL4^repo.PU/Pcb^UAS.cYa

(Yamazaki et al., 2014)

Pka-C1^B3 has abnormal neuroanatomy phenotype, suppressible by Mmus\Prkaca^mC.UAS/Scer\GAL4^Toll-6-D42

(Yoshihara et al., 2005)

Enhancer of

Statement

Reference

Pka-C1^B3 is an enhancer of decreased body size | pupal stage phenotype of Nf1^E2

(Walker et al., 2013)

Suppressor of

Statement

Reference

Pka-C1^B3 is a suppressor of hyperplasia | conditional | somatic clone phenotype of Ras85D^V12.UAS, Scer\GAL4^FRT.Act5C, Scer\GAL80^αTub84B.PL

(Zeng et al., 2010)

Phenotype Manifest In

Enhanced by

Statement

Reference

Pka-C1^B3 has wing phenotype, enhanceable by Su(fu)^LP

(Ohlmeyer and Kalderon, 1998)

Pka-C1^B3 has wing vein phenotype, enhanceable by Su(fu)^LP

(Ohlmeyer and Kalderon, 1998)

NOT Enhanced by

Statement

Reference

Pka-C1^B3 has perineurium | embryonic stage phenotype, non-enhanceable by Gαi^P8

(Li et al., 2021)

Pka-C1^B3 has perineurium | embryonic stage phenotype, non-enhanceable by Gαo⁰⁶¹¹

(Li et al., 2021)

Pka-C1^B3 has perineurium | embryonic stage phenotype, non-enhanceable by Rho1^1B

(Li et al., 2021)

Pka-C1^B3 has perineurium | embryonic stage phenotype, non-enhanceable by Rho1^72R

(Li et al., 2021)

Pka-C1^B3 has wing phenotype, non-enhanceable by fu^mH63

(Ohlmeyer and Kalderon, 1998)

Suppressed by

Statement

Reference

Pka-C1^B3 has perineurium | embryonic stage phenotype, suppressible by loco^Δ13

(Li et al., 2021)

Pka-C1^B3 has perineurium | embryonic stage phenotype, suppressible by Strn-Mlck^c02860

(Li et al., 2021)

Pka-C1^B3 has perineurium | embryonic stage phenotype, suppressible by Strn-Mlck^C234

(Li et al., 2021)

Pka-C1^B3 has perineurium | embryonic stage phenotype, suppressible by Rho1^E3.10

(Li et al., 2021)

Pka-C1^B3 has motor neuron phenotype, suppressible by Mmus\Prkaca^mC.UAS/Scer\GAL4^Toll-6-D42

(Yoshihara et al., 2005)

NOT suppressed by

Statement

Reference

Pka-C1^B3 has perineurium | embryonic stage phenotype, non-suppressible by Gαi^P8

(Li et al., 2021)

Pka-C1^B3 has perineurium | embryonic stage phenotype, non-suppressible by Gαo⁰⁶¹¹

(Li et al., 2021)

Pka-C1^B3 has perineurium | embryonic stage phenotype, non-suppressible by Rho1^1B

(Li et al., 2021)

Pka-C1^B3 has perineurium | embryonic stage phenotype, non-suppressible by Rho1^72R

(Li et al., 2021)

Pka-C1^B3 has wing phenotype, non-suppressible by fu^mH63

(Ohlmeyer and Kalderon, 1998)

Suppressor of

Statement

Reference

Pka-C1^B3 is a suppressor of eye phenotype of hid^GMR.PG

(Christiansen et al., 2013)

Pka-C1^B3 is a suppressor of adult Malpighian tubule | conditional | somatic clone phenotype of Ras85D^V12.UAS, Scer\GAL4^FRT.Act5C, Scer\GAL80^αTub84B.PL

(Zeng et al., 2010)

Other

Statement

Reference

Pka-C1^B3, Su(H)^del47, hid^GMR.PG has eye phenotype

(Christiansen et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

Overexpression of PCB^Scer\UAS.cYa in glia (Scer\GAL4^repo.PU) restores age-related memory impairment in Pka-C1^B3/+ flies.

(Yamazaki et al., 2014)

Eyes mosaic for Pka-C1^B3 (generated using the ey-FLP/FRT system) moderately suppress the W^GMR.PG eye phenotype. This suppression is cell non-autonomous.

The suppression of the W^GMR.PG eye phenotype seen in eyes mosaic for Pka-C1^B3 (generated using the ey-FLP/FRT system) is partially reverted if these clones are also mutant for Su(H)^del47.

(Christiansen et al., 2013)

The mSC frequency at the larval neuromuscular junction is generally lower in Mhc¹ Pka-C1^B3 larvae than in Mhc¹ larvae. The mean amplitude of the mSCs and nerve-evoked synaptic currents in the double mutant larvae are not different from those in Mhc¹ larvae.

(Yoshihara et al., 2000)

Wing phenotype of Pka-C1^B3 mutant clones is unaffected by fu^mH63.

(Ohlmeyer and Kalderon, 1998)

Xenogenetic Interactions

Statement

Reference

Expression of Mmus\Pkaca^mC.Scer\UAS under the control of Scer\GAL4^D42 restores the normal constricted varicosities at the motor terminals of the Pka-C1^B3 neuromuscular junction.

(Yoshihara et al., 2005)

Complementation and Rescue Data

Rescued by

Pka-C1^B3 is rescued by Pka-C1^UAS.Tag:FLAG/Scer\GAL4^Tab2-201Y

(Horiuchi et al., 2008)

Pka-C1^B3 is rescued by Scer\GAL4^c747/Pka-C1^UAS.Tag:FLAG

(Horiuchi et al., 2008)

Pka-C1^B3 is rescued by Pka-C1^+mTgDC0

(Lane and Kalderon, 1993)

Comments

One copy of Pka-C1^+mTgDC0 fails to rescue hemizygotes, but two copies provides full rescue.

(Lane and Kalderon, 1993)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Strong Pka-C1 allele.

(Lane and Kalderon, 1993)

Clonal analysis indicates that Pka-C1 acts autonomously in the germline.

(Lane and Kalderon, 1995)

In Pka-C1^- cells, in the anterior compartment of the wing and leg discs, genes that are normally restricted to the anterior-posterior border cells are ectopically activated, but the cells retain the dorsal-ventral positional information.

(Pan and Rubin, 1995)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (14)

Reported As

Symbol Synonym

DC0^B3

(Matsuno et al., 2019, Yamazaki et al., 2014, Horiuchi et al., 2008, Yamazaki et al., 2007, Yoshihara et al., 2005, Yoshihara et al., 2000, The et al., 1997, Li et al., 1996, Sanchez-Herrero et al., 1996, Lane and Kalderon, 1994, Levine et al., 1994)

DCO^B3

(Sakai et al., 2004, Estrada and Sanchez-Herrero, 2001, Asha et al., 1999, Moore et al., 1998, Ohlmeyer and Kalderon, 1998)

Dco^B3

(Zeng et al., 2010)

PKA-C1^B3

(Walker et al., 2013, Zhou et al., 2006)

PKA^B3

(Copf, 2014)

PKAC1^B3

(Graves et al., 2015)

Pka-C1^B3

(Marks and Kalderon, 2011)

Pka^B3

(Chen and Baker, 1997, Pan and Rubin, 1995)

PkaC1^B3

(Li et al., 2021)

dc0^B3

(Kanellopoulos et al., 2012)

pka-C1^B3

(Hsia et al., 2017, Christiansen et al., 2013, Christiansen et al., 2012, Zheng et al., 2010, Glise et al., 2002)

pka^B3-DC0

(Dominguez, 1999)

pka^B3

(de Celis et al., 1999, Gomez-Skarmeta and Modolell, 1996)

pka^DC0-B3

(Dominguez and Hafen, 1997)

Name Synonyms

Secondary FlyBase IDs

References (41)

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