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General Information
Symbol
Dmel\Pka-C1B3
Species
D. melanogaster
Name
FlyBase ID
FBal0033955
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
DC0B3, DCOB3, pkaB3
Mutagen
    Nature of the Allele
    Mutagen
    Mutations Mapped to the Genome
     
    Type
    Location
    Additional Notes
    References
    point mutation
    Nucleotide change:

    G9696671A

    Reported nucleotide change:

    G3019A

    Amino acid change:

    W299term | Pka-C1-PB; W299term | Pka-C1-PC; W299term | Pka-C1-PD

    Reported amino acid change:

    W299term

    Associated Sequence Data
    DNA sequence
    Protein sequence
     
     
    Progenitor genotype
    Cytology
    Nature of the lesion
    Statement
    Reference

    Amino acid replacement: W299term.

    Expression Data
    Reporter Expression
    Additional Information
    Statement
    Reference
     
    Marker for
    Reflects expression of
    Reporter construct used in assay
    Human Disease Associations
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 1 )
    Disease
    Interaction
    References
    Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
     
    Phenotypic Data
    Phenotypic Class
    Phenotype Manifest In
    Detailed Description
    Statement
    Reference

    The class IV dendritic arborization neurons in Pka-C1B3 mutant clones show a significant decrease in the complexity of dendritic arbors in respect to the total dendritic length.

    Pka-C1B3/+ flies do not show age-related memory impairment seen in wild type.

    Pka-C1B3/+ heterozygotes have dramatically improved memory retention curves compared with wild-type flies. Avoidance of naive flies to the odors odors and electrical shocks used during training is not significantly different between wild-type and Pka-C1B3 heterozygous mutants, indicating that the observed increases in memory are not caused by increased sensitivity to these stimuli. Memory retention curves of Pka-C1B3 /+ heterozygotes indicate that early forms of memory, including memory tested immediately after training (3-min memory) and short forms of memory (1-h memory) are not greatly affected. However, memory at later time points, 3 h and 7 h after training, progressively increases relative to wild-type, such that at 7h, memory is approximately double that of wild-type.

    Pka-C1B3/+ flies have improved cold shock-resistant 3-h memory, indicating that anesthesia-resistant memory (ARM) is increased in these flies. Pka-C1B3/+ heterozygote show increased 24-h memory after massed training, indicating increased ARM production.

    Compared to wild-types, long-term memory is not altered in Pka-C1B3/+ heterozygotes.

    Memory enhancement in Pka-C1B3/+ flies begins between 1 and 3 h after single cycle training and reverts to normal within 4 days after spaced training.

    Expression of Pka-C1Scer\UAS.T:Zzzz\FLAG using a mushroom body driver (Scer\GAL4c309) abolishes 24-h memory after massed training in a Pka-C1B3/+ heterozygous background.

    High-frequency-stimulation-induced miniature release (HFMR) is eliminated in the neuromuscular junctions of Pka-C1B3 animals, in contrast to controls.

    Unlike wild-type males, Pka-C1B3/+ males that have undergone courtship conditioning (kept in the presence of a female for 7 hours) do not spend significantly less time engaged in courtship behaviour when placed with a female 5 days after conditioning than non-conditioned males of the same genotype.

    Forskolin does not alter the mSC frequency at the larval neuromuscular junction in mutants.

    Heterozygotes show increased sensitivity to ethanol in an inebriometer assay.

    Clones induce pattern duplications in adult female internal genitalia and female external terminalia and duplications in the adult male terminalia.

    Pka-C1B3/Pka-C1Tw2 transheterozygotes are phenotypically similar to Nf1 mutants, small body size.

    Pka-C1B3/Pka-C1Tw2 females are sterile and contain multinucleate nurse cells in egg chambers beyond stage 5. Discontinuities in the actin ring at the site of the intercellular bridges are seen. Border follicle cells sometimes do not migrate or show incomplete migration in Pka-C1B3/Pka-C1Tw2 females.

    Heterozygotes are wild type. Homozygous mutant clones in the anterior compartment of the legs and wings causes pattern abnormalities, duplicated wing with mirror symmetry through the centre of the clone and sometimes supernumerary legs.

    Homozygous germline clones generate egg chambers with numerous nurse cell fusions.

    Heterozygotes exhibit rhythmic locomotor activity in constant darkness and the average circadian rhythm is slightly shorter than wild type. Heterozygotes had ostensibly normal behaviour.

    Hemizygotes are larval lethal. Mature oocytes are smaller than wild type and nurse cells are multinucleate.

    External Data
    Interactions
    Show genetic interaction network for Enhancers & Suppressors
    Phenotypic Class
    Suppressed by
    Enhancer of
    Statement
    Reference

    Pka-C1B3 is an enhancer of small body | pupal stage phenotype of Nf1E2

    Suppressor of
    Phenotype Manifest In
    Enhanced by
    Statement
    Reference

    Pka-C1B3 has wing phenotype, enhanceable by Su(fu)LP

    NOT Enhanced by
    Statement
    Reference

    Pka-C1B3 has wing phenotype, non-enhanceable by fumH63

    Suppressed by
    Statement
    Reference
    NOT suppressed by
    Statement
    Reference

    Pka-C1B3 has wing phenotype, non-suppressible by fumH63

    Suppressor of
    Other
    Statement
    Reference
    Additional Comments
    Genetic Interactions
    Statement
    Reference

    Overexpression of PCBScer\UAS.cYa in glia (Scer\GAL4repo.PU) restores age-related memory impairment in Pka-C1B3/+ flies.

    Eyes mosaic for Pka-C1B3 (generated using the ey-FLP/FRT system) moderately suppress the WGMR.PG eye phenotype. This suppression is cell non-autonomous.

    The suppression of the WGMR.PG eye phenotype seen in eyes mosaic for Pka-C1B3 (generated using the ey-FLP/FRT system) is partially reverted if these clones are also mutant for Su(H)del47.

    The mSC frequency at the larval neuromuscular junction is generally lower in Mhc1 Pka-C1B3 larvae than in Mhc1 larvae. The mean amplitude of the mSCs and nerve-evoked synaptic currents in the double mutant larvae are not different from those in Mhc1 larvae.

    Wing phenotype of Pka-C1B3 mutant clones is unaffected by fumH63.

    Xenogenetic Interactions
    Statement
    Reference

    Expression of Mmus\PkacamC.Scer\UAS under the control of Scer\GAL4D42 restores the normal constricted varicosities at the motor terminals of the Pka-C1B3 neuromuscular junction.

    Complementation and Rescue Data
    Comments

    One copy of Pka-C1+mTgDC0 fails to rescue hemizygotes, but two copies provides full rescue.

    Images (0)
    Mutant
    Wild-type
    Stocks (0)
    Notes on Origin
    Discoverer
    Comments
    Comments

    Clonal analysis indicates that Pka-C1 acts autonomously in the germline.

    In Pka-C1- cells, in the anterior compartment of the wing and leg discs, genes that are normally restricted to the anterior-posterior border cells are ectopically activated, but the cells retain the dorsal-ventral positional information.

    External Crossreferences and Linkouts ( 0 )
    Synonyms and Secondary IDs (12)
    References (37)