The additional expression of mib1Scer\UAS.T:Zzzz\His6,T:Hsap\MYC in wing discs expressing SerScer\UAS.cSa under the control of Scer\GAL4dpp.blk1 does not change the wing disc phenotype. In contrast, the wing phenotype caused by SerScer\UAS.cSa under the control of Scer\GAL4dpp.blk1 is completely suppressed by mib1ΔRF.Scer\UAS.T:Zzzz\His6,T:Hsap\MYC coexpression.
The following heterozygous mutations suppress the large eye phenotype of Scer\GAL4ey.PH > SerScer\UAS.cSa flies: eyg685, Df(1)N-54l9, upd154 and hop25. Heterozygosity for either domeG0218, domeG0282 or Stat92Ej6C8 fails to suppress this phenotype.
Co-expression of both tshScer\UAS.cGa and SerScer\UAS.cSa under the control of Scer\GAL4bi-omb-Gal4 suppresses eye development in both the dorsal and ventral margins of the eye disc and adult eye. However, despite this suppression of photoreceptor differentiation, the eye disc shows overall enlargement and the adult eye is also enlarged and folded.
The wing phenotype caused by expression of SerScer\UAS.cSa under the control of Scer\GAL4ptc-559.1 is suppressed by coexpression of NECN.Scer\UAS, NECNΔ10-12.Scer\UAS or NECNΔ17-19;24-26.Scer\UAS (although suppression is not always complete in the case of NECNΔ17-19;24-26.Scer\UAS).
Chie5.5 clones in the wing disc of animals expressing SerScer\UAS.cSa under the control of Scer\GAL4Bx-MS1096 cause axis bifurcation. The severity of the BxhdpR590/Y wing phenotype is enhanced by expression of SerScer\UAS.cSa under the control of Scer\GAL4Bx-hdpR590.
apmd544/apUGO35 flies show reduction of the wing and complete loss of wing margin. Expression of SerScer\UAS.cSa under the control of Scer\GAL4ap-md544 in these flies restores the wing margin and supports growth of the wing. Patches of ectopic wing margin are found in both surfaces of the rescued wings and overgrowth of the wing can be seen. Groups of cells appear to violate the dorsoventral boundary in apmd544/apUGO35 wing discs which are also expressing SerScer\UAS.cSa under the control of Scer\GAL4ap-md544. The mispositioned patches of dorsal or ventral cells form relatively smooth interfaces with surrounding cells. Expression of both SerScer\UAS.cSa and fngEP3082 under the control of Scer\GAL4ap-md544 in apmd544/apUGO35 flies results in well-developed wings which differentiate ventral features on both surfaces; the wing margin, veins and sense organs on the topologically dorsal surface have exclusively ventral identity.
The ectopic wing margin structures seen in flies expressing SerScer\UAS.cSa under the control of Scer\GAL4ptc-559.1 are reduced to a posterior stripe with characteristics of the posterior compartment if the flies are also co-expressing fngScer\UAS.cKa. The loss of wing seen in apUGO35 homozygotes is partially rescued by SerScer\UAS.cSa expressed under the control of Scer\GAL4dpp.blk1. The extra vein phenotype seen in flies expressing SerScer\UAS.cSa under the control of Scer\GAL4Bx-MS1096 is suppressed if the flies are also expressing fngScer\UAS.cKa.
Mediated expression in apUGO35 mutants elicits an ectopic wing. Wing consists of dorsal and ventral cells and the wing margin cells are on the dorsal side.
Coexpression of NECN.Scer\UAS and SerScer\UAS.cSa under the control of Scer\GAL4sd-SG29.1 demonstrates Ser can partially suppress the effect of N : wings are smaller than wild type, the dorsal surface is smaller than the ventral surface. Coexpression of wgl-12.Scer\UAS and SerScer\UAS.cSa with Scer\GAL4sd-SG29.1 the resulting flies bear large wings that are covered in bristles. The wings lack a defined margin and are more round that elongated along the PD axis. Coexpression with Scer\GAL4ptc-559.1 leads to the production of extra wings off the proximal dorsal side of the wing. Coexpression of SerScer\UAS.cSa and NScer\UAS.cBb under the control of Scer\GAL4sd-SG29.1 generates very large wings with a clearly defined margin with an antineurogenic phenotype. Coexpression under the control of Scer\GAL4Bx-MS1096 results in normally shaped wings in which the dorsal surface has overgrown the ventral one. Coexpression under the control of Scer\GAL4ptc-559.1 causes the ectopic ventral double margin characteristic of SerScer\UAS.cSa alone to be transformed into a single thick one, and the ventral margin and associated overgrowth are extended into the dorsal surface of the wing.
The ectopic wing margin phenotype caused by expression of SerScer\UAS.cSa under the control of Scer\GAL4ptc-559.1 is partly suppressed in flies also carrying fngD4. Co-expression of fngScer\UAS.cKa with SerScer\UAS.cSa in the wing (using Scer\GAL4ptc-559.1) stimulates ectopic wing margin formation.
Scer\GAL4ptc-559.1-mediated coexpression with NECN.Scer\UAS allows most wild-type flies to hatch at 25oC, both phenotypes provoked by SerScer\UAS.cSa alone are strongly suppressed. Scer\GAL4ptc-559.1-mediated coexpression with wgl-12.Scer\UAS causes outgrowth on the ventral side of the wing discs and a small amount on the dorsal side but the nick in the wing margin is repressed. Ectopic wing tissue is also found in the compound eye and legs. Scer\GAL4h-540.3-mediated expression of SerScer\UAS.cSa in apunspecified second/early third larval instar partially restores wing formation (about half normal length but lack any marginal differentiation).
Scer\GAL4ptc-559.1 induced co-expression of wgl-12.Scer\UAS and SerScer\UAS.cSa causes a duplicated pair of extra wings that arise from the proximal dorsal side of the endogenous wings. The endogenous wings may exhibit extra margin material. Wing tissues appear, with characteristic marginal bristles, from the proximal dorsal side in all legs. Scer\GAL4h-1J3 induced coexpression of wgl-12.Scer\UAS and SerScer\UAS.cSa fails to cause ectopic wings, expression of NScer\UAS.cBb with both wgl-12.Scer\UAS and SerScer\UAS.cSa causes ectopic wing development.