FlyBase Allele Report: Dmel\dpp[UAS.cSa]

General Information

Symbol

Dmel\dpp^UAS.cSa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Staehling-Hampton

FlyBase ID

FBal0034429

Feature type

allele

Associated gene

Dmel\dpp

Associated Insertion(s)

Carried in Construct

P{UAS-dpp.S}

Also Known As

UAS-dpp, UAS.Dpp, UAS-dpp.S, UAS dpp, UASdpp, UAS-dpp^42B.4, UAS::Dpp

Key Links

Transgenic product class

cDNA

(Staehling-Hampton et al., 1994)

wild_type

(Staehling-Hampton et al., 1994)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Staehling-Hampton et al., 1994)

wild_type

(Staehling-Hampton et al., 1994)

Mutagen

in vitro construct

(Bilder and Scott, 1998, Carmena et al., 1998, Michelson et al., 1998, de Celis, 1997, Staehling-Hampton et al., 1994, Staehling-Hampton et al., 1994)

Progenitor genotype

Carried in construct

P{UAS-dpp.S}

Cytology

Description

UASt regulatory sequences drive expression of a dpp cDNA.

(Staehling-Hampton et al., 1994)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dpp

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell migration, with Scer\GAL4^twi.PG

(Deshpande et al., 2014)

abnormal cell migration, with Scer\GAL4^{VP16.nanos.UTR}

(Deshpande et al., 2014)

abnormal size, with Scer\GAL4^dpp.blk1

(Kopp et al., 1999)

abnormal size, with Scer\GAL4^T155

(Kopp et al., 1999)

decreased occurrence of cell division | larval stage, with Scer\GAL4^hs.PB

(Horsfield et al., 1998)

hyperplasia, with Scer\GAL4^C-765

(Nellen et al., 1996)

hyperplasia | adult stage, with Scer\GAL4^C587

(Xia et al., 2012)

hyperplasia | third instar larval stage, with Scer\GAL4^vg.int2.1

(Ibrahim et al., 2013)

increased cell death, with Scer\GAL4^{VP16.nanos.UTR}

(Schulz et al., 2004, Shivdasani and Ingham, 2003)

increased cell number | spermatogenesis, with Scer\GAL4^C587

(Zhang et al., 2017)

increased occurrence of cell division, with Scer\GAL4^C-765

(Nellen et al., 1996)

lethal, with Scer\GAL4^ptc-559.1

(Pignoni and Zipursky, 1997)

lethal, with Scer\GAL4^sca-375.1

(Pignoni and Zipursky, 1997)

lethal, with Scer\GAL4^sca-537.4

(Quijano et al., 2011)

lethal, with Scer\GAL4^unspecified

(Takaesu et al., 2005)

lethal, with Scer\GAL4^upd1-E132

(Pignoni and Zipursky, 1997)

lethal | heat sensitive, with Scer\GAL4^hs.PH

(Muzzopappa and Wappner, 2005)

lethal - all die before end of larval stage, with Scer\GAL4^how-24B

(Staehling-Hampton and Hoffmann, 1994)

lethal - all die during embryonic stage, with Scer\GAL4^nanos.PG

(Tracey et al., 2000)

lethal - all die during embryonic stage, with Scer\GAL4^rho.PL

(Oh et al., 2002)

some die during embryonic stage, with Scer\GAL4^nanos.PG

(Tracey et al., 2000)

some die during embryonic stage, with Scer\GAL4^rho.PL

(Oh et al., 2002)

some die during larval stage, with Scer\GAL4^how-24B

(Staehling-Hampton and Hoffmann, 1994)

visible, with Scer\GAL4^30A

(Haines and van Den Heuvel, 2000)

visible, with Scer\GAL4^dpp.3KK

(Molnar et al., 2006)

visible, with Scer\GAL4^dpp.blk1

(Kopp et al., 1999)

visible, with Scer\GAL4^T155

(Kopp et al., 1999)

visible, with Scer\GAL4^αTub84B.PP

(Kopp et al., 1999)

Phenotype Manifest In

abdominal sternite, with Scer\GAL4^dpp.blk1

(Kopp et al., 1999)

abdominal sternite, with Scer\GAL4^T155

(Kopp et al., 1999)

abdominal sternite, with Scer\GAL4^αTub84B.PP

(Kopp et al., 1999)

abdominal tergite, with Scer\GAL4^dpp.blk1

(Kopp et al., 1999)

abdominal tergite, with Scer\GAL4^T155

(Kopp et al., 1999)

abdominal tergite, with Scer\GAL4^αTub84B.PP

(Kopp et al., 1999)

actin filament & tracheal tip cell & embryo, with Scer\GAL4^btl.PS

(Kato et al., 2004)

adult subretinal glial cell | ectopic, with Scer\GAL4^GMR.PF

(Rangarajan et al., 2001)

amnioserosa, with Scer\GAL4^da.G32

(Chang et al., 2001)

antennal disc, with Scer\GAL4^hs.PB

(Horsfield et al., 1998)

cyst progenitor cell | spermatogenesis, with Scer\GAL4^C587

(Zhang et al., 2017)

dorsal appendage, with Scer\GAL4^c532

(Dequier et al., 2001, Deng and Bownes, 1997)

dorsal appendage, with Scer\GAL4^CY2

(Shravage et al., 2007)

dorsal appendage, with Scer\GAL4^GR1

(Shravage et al., 2007)

dorsal appendage, with Scer\GAL4^hs.PB

(Dobens et al., 2000)

dorsal appendage, with Scer\GAL4^hs.PH

(Muzzopappa and Wappner, 2005)

dorsal appendage | increased number, with Scer\GAL4^c532

(Dequier et al., 2001)

dorsal mesothoracic disc primordium, with Scer\GAL4^Dll-md23

(Kubota et al., 2000)

egg operculum, with Scer\GAL4^c532

(Dequier et al., 2001)

egg operculum, with Scer\GAL4^CY2

(Shravage et al., 2007)

egg operculum, with Scer\GAL4^hs.PB

(Dobens et al., 2000)

egg operculum, with Scer\GAL4^hs.PH

(Muzzopappa and Wappner, 2005)

embryonic/first instar larval cuticle | ventral, with Scer\GAL4^69B

(Byars et al., 1999)

embryonic/first instar larval cuticle | ventral, with Scer\GAL4^rho.PL

(Oh et al., 2002)

embryonic/larval crystal cell, with Scer\GAL4^twi.PG

(Milchanowski et al., 2004)

embryonic/larval fat body & parasegment 4, with Scer\GAL4^twi.PGa

(Riechmann et al., 1998)

embryonic/larval fat body & parasegment 5, with Scer\GAL4^twi.PGa

(Riechmann et al., 1998)

embryonic/larval fat body & parasegment 6, with Scer\GAL4^twi.PGa

(Riechmann et al., 1998)

embryonic/larval fat body & parasegment 6, with Scer\GAL4^zen.Kr.PF

(Riechmann et al., 1998)

embryonic/larval fat body & parasegment 7, with Scer\GAL4^twi.PGa

(Riechmann et al., 1998)

embryonic/larval fat body & parasegment 7, with Scer\GAL4^zen.Kr.PF

(Riechmann et al., 1998)

embryonic/larval fat body & parasegment 8, with Scer\GAL4^twi.PGa

(Riechmann et al., 1998)

embryonic/larval fat body & parasegment 8, with Scer\GAL4^zen.Kr.PF

(Riechmann et al., 1998)

embryonic/larval fat body & parasegment 9, with Scer\GAL4^twi.PGa

(Riechmann et al., 1998)

embryonic/larval fat body & parasegment 10, with Scer\GAL4^twi.PGa

(Riechmann et al., 1998)

embryonic/larval fat body & parasegment 11, with Scer\GAL4^twi.PGa

(Riechmann et al., 1998)

embryonic/larval fat body & parasegment 12, with Scer\GAL4^twi.PGa

(Riechmann et al., 1998)

embryonic/larval tracheal dorsal trunk, with Scer\GAL4^btl.PS

(Beitel and Krasnow, 2000, Wappner et al., 1997)

embryonic/larval visceral tracheal branch, with Scer\GAL4^btl.PS

(Wappner et al., 1997)

embryonic anterior Malpighian tubule, with Scer\GAL4^cad-md509

(Bunt et al., 2010)

embryonic anterior Malpighian tubule, with Scer\GAL4^en-e16E

(Bunt et al., 2010)

embryonic epidermis, with Scer\GAL4^en-e16E

(Arquier et al., 2001)

embryonic epidermis, with Scer\GAL4^prd.RG1

(Arquier et al., 2001)

embryonic head epidermis, with Scer\GAL4^da.G32

(Chang et al., 2001)

embryonic hindgut, with Scer\GAL4^byn-Gal4

(Iwaki and Lengyel, 2002)

embryonic hindgut, with Scer\GAL4^ptc-559.1

(Takashima and Murakami, 2001)

embryonic midgut constriction 1, with Scer\GAL4^how-24B

(Szuts et al., 1998, Wisotzkey et al., 1998)

embryonic midgut constriction 2, with Scer\GAL4^how-24B

(Szuts et al., 1998, Wisotzkey et al., 1998)

embryonic midgut constriction 3, with Scer\GAL4^how-24B

(Szuts et al., 1998, Wisotzkey et al., 1998)

epidermis | dorsal, with Scer\GAL4^hs.PB

(Bokor and DiNardo, 1996)

epidermis | dorsal, with Scer\GAL4^ptc-559.1

(Bokor and DiNardo, 1996)

eye disc, with Scer\GAL4^hs.PB

(Horsfield et al., 1998)

eye disc | somatic clone, with Scer\GAL4^Act5C.PI

(Baonza and Freeman, 2001)

eye disc morphogenetic furrow | ectopic, with Scer\GAL4^upd1-E132

(Pignoni and Zipursky, 1997)

fat body/gonad primordium | ectopic, with Scer\GAL4^twi.PGa

(Riechmann et al., 1998)

female germline cell | increased number, with Scer\GAL4^C587

(Xia et al., 2012)

female germline stem cell, with Scer\GAL4^hs.PH

(Muzzopappa and Wappner, 2005)

germarium, with Scer\GAL4^C587

(Xia et al., 2012)

germinal proliferation center | spermatogenesis, with Scer\GAL4^C587

(Zhang et al., 2017)

germline cell, with Scer\GAL4^twi.PG

(Deshpande et al., 2014)

germline cell, with Scer\GAL4^{VP16.nanos.UTR}

(Deshpande et al., 2014)

germline cell | male, with Scer\GAL4^{VP16.nanos.UTR}

(Schulz et al., 2004)

glial cell & eye | ectopic, with Scer\GAL4^Act5C.PP

(Rangarajan et al., 2001)

glial cell & eye | ectopic, with Scer\GAL4^GMR.PF

(Rangarajan et al., 2001)

gonad, with Scer\GAL4^twi.PG

(Deshpande et al., 2014)

gonad, with Scer\GAL4^{VP16.nanos.UTR}

(Deshpande et al., 2014)

hub cell, with Scer\GAL4^{VP16.nanos.UTR}

(Schulz et al., 2004)

labral segment, with Scer\GAL4^da.G32

(Chang et al., 2001)

male germline stem cell | increased number, with Scer\GAL4^{VP16.nanos.UTR}

(Schulz et al., 2004)

male germline stem cell | spermatogenesis, with Scer\GAL4^C587

(Zhang et al., 2017)

mesothoracic leg, with Scer\GAL4^dpp.blk1

(Morimura et al., 1996)

metathoracic leg, with Scer\GAL4^dpp.blk1

(Morimura et al., 1996)

microchaeta | ectopic, with Scer\GAL4^69B

(Staehling-Hampton et al., 1994)

midgut, with Scer\GAL4^how-24B

(Staehling-Hampton and Hoffmann, 1994)

ovary, with Scer\GAL4^C587

(Xia et al., 2012)

parasegment 13 & mesoderm | ventral, with Scer\GAL4^twi.PGa

(Riechmann et al., 1998)

photoreceptor neuron | ectopic, with Scer\GAL4^Act5C.PP

(Rangarajan et al., 2001)

pleurum, with Scer\GAL4^dpp.blk1

(Kopp et al., 1999)

pleurum, with Scer\GAL4^T155

(Kopp et al., 1999)

primary gonial cell, with Scer\GAL4^{VP16.nanos.UTR}

(Schulz et al., 2004)

primary gonial cell | spermatogenesis, with Scer\GAL4^C587

(Zhang et al., 2017)

prothoracic leg, with Scer\GAL4^dpp.blk1

(Morimura et al., 1996)

pseudotrachea, with Scer\GAL4^pb.PJ

(Joulia et al., 2005)

scutellum, with Scer\GAL4^dpp.blk1

(Morimura et al., 1996)

scutellum & macrochaeta, with Scer\GAL4^dpp.blk1

(Morimura et al., 1996)

spermatid, with Scer\GAL4^{VP16.nanos.UTR}

(Shivdasani and Ingham, 2003)

spermatocyte, with Scer\GAL4^{VP16.nanos.UTR}

(Schulz et al., 2004, Shivdasani and Ingham, 2003)

spermatogonium, with Scer\GAL4^{VP16.nanos.UTR}

(Schulz et al., 2004)

spermatogonium | increased number, with Scer\GAL4^{VP16.nanos.UTR}

(Shivdasani and Ingham, 2003)

spiracular chamber, with Scer\GAL4^arm.PS

(Hu and Castelli-Gair, 1999)

testis, with Scer\GAL4^{VP16.nanos.UTR}

(Shivdasani and Ingham, 2003)

tracheal tip cell | embryonic stage, with Scer\GAL4^btl.PS

(Kato et al., 2004)

ventral denticle belt, with Scer\GAL4^69B

(Nellen et al., 1996, Staehling-Hampton et al., 1994)

ventral denticle belt, with Scer\GAL4^nanos.PG

(Tracey et al., 2000)

visual primordium, with Scer\GAL4^da.G32

(Chang et al., 2001)

wing, with Scer\GAL4^30A

(Haines and van Den Heuvel, 2000)

wing, with Scer\GAL4^C-580

(de Celis, 1997)

wing, with Scer\GAL4^dpp.3KK

(Molnar et al., 2006)

wing | posterior, with Scer\GAL4^dpp.blk1

(Morimura et al., 1996)

wing disc, with Scer\GAL4^30A

(Minami et al., 1999)

wing disc, with Scer\GAL4^ap-md544

(Nfonsam et al., 2012)

wing disc, with Scer\GAL4^C-765

(Nellen et al., 1996)

wing disc, with Scer\GAL4^dpp.blk1

(Burke and Basler, 1996)

wing disc, with Scer\GAL4^vg.int2.1

(Klein and Martinez Arias, 1999)

wing disc | somatic clone, with Scer\GAL4^unspecified

(Szuperák et al., 2011)

wing disc | third instar larval stage, with Scer\GAL4^vg.int2.1

(Ibrahim et al., 2013)

wing vein | ectopic, with Scer\GAL4^C-580

(de Celis, 1997)

wing vein | ectopic, with Scer\GAL4^dpp.3KK

(Molnar et al., 2006)

Detailed Description

Statement

Reference

The adulthood-only expression of dpp^UAS.cSa under the control of Scer\GAL4^C587 and Gal80[ts] leads to adult testes exhibiting significant increases in the numbers of both cyst stem cells and early stage germline cells, as compared to controls.

(Zhang et al., 2017)

Expression of dpp^Scer\UAS.cSa under the control of Scer\GAL4^twi.PG results in a substantial reduction in the number of primordial germ cells (PGCs)in the coalesced gonad. PGCs also display migration defects, with a significant fraction scattering through the embryo instead of making contact with the somatic gonad precursor cells.

Expression of dpp^Scer\UAS.cSa in the germline, under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16}, does not generate any abnormalities in somatic development. There are little if any defects in germ cell migration and most primordial germ cells are properly aligned with the somatic germ cell precursors in stage 13 embryos. These mutants exhibit a substantial reduction in the number of germ cells on the coalesced gonad, apparently due to apoptosis.

(Deshpande et al., 2014)

Expression of dpp^Scer\UAS.cSa under the control of Scer\GAL4^vg.int2.1 results in extensive overproliferation in both anterior and posterior compartment cells in the wing disc.

(Ibrahim et al., 2013)

Expression of dpp^Scer\UAS.cSa under the control of Scer\GAL4^ap-md544 results in an increase in size of the wing disc.

(Nfonsam et al., 2012)

Expression of dpp^Scer\UAS.cSa in inner germarium sheath (IGS) cells under the control of Scer\GAL4^C587 results in tumorous germaria that are filled with bam-negative GSC-like cells.

(Xia et al., 2012)

Clones in the wing disc expressing dpp^Scer\UAS.cSa under the control of Scer\GAL4^unspecified show overgrowth.

(Szuperák et al., 2011)

Expression of dpp^Scer\UAS.cSa in every epidermal segment under the control of Scer\GAL4^en-e16E, or in the posterior gut under the control of Scer\GAL4^cad-md509, both result in mis-routing of the anterior pair of Malpighian tubules towards the ectopic source of dpp.

(Bunt et al., 2010)

Females expressing dpp^Scer\UAS.cSa under the control of Scer\GAL4^GR1 produce eggs with enlarged dorsal appendages that are shifted towards the posterior compared to wild-type eggs.

Females expressing dpp^Scer\UAS.cSa under the control of Scer\GAL4^CY2 produce eggs showing an expansion of the operculum at the expense of the dorsal appendages.

(Shravage et al., 2007)

Expression of dpp^Scer\UAS.cSa under the control of Scer\GAL4^dpp.3KK results most of the wing tissue differentiating as vein.

(Molnar et al., 2006)

The wings of dpp^Scer\UAS.cSa; Scer\GAL4^dpp.3KK flies are covered in ectopic vein tissue. This phenotype is rescued by dpp^s22/dpp^s4. The resulting flies have a milder shortvein phenotype than dpp^s22/dpp^s4 flies.

(Sotillos and de Celis, 2006)

Overexpression of dpp^Scer\UAS.cSa under the control of Scer\GAL4^pb.PJ leads to extensive loss of pseudotracheae in the labial palps.

(Joulia et al., 2005)

Mothers that express dpp^Scer\UAS.cSa, driven by Scer\GAL4^hs.PH following a heat shock, produce eggs that in 89% of cases have eggshells with giant opercula and reduced or absent dorsal appendages. Germaria from these females contain tumorous arrays of germline stem cells and aberrant follicles with large numbers of germ cells engulfed in the same follicular epithelium.

(Muzzopappa and Wappner, 2005)

Eggs laid by dpp^Scer\UAS.cSa; Scer\GAL4^{l(1)3At-PG150} mothers have an expanded operculum and a more posterior placement of the dorsal appendage bases.

(Ward and Berg, 2005)

Expression of dpp^UAS.cSa, under the control of either Scer\GAL4^ChAT.7.4, or Scer\GAL4^eve.RN2 has no effect on the synaptic current amplitude in aCC/RP2 neurons compared to controls.

(Baines, 2004)

In dpp^Scer\UAS.cSa; Scer\GAL4^btl.PS embryos, terminal branch extension stalls. The actin core in these stalled branches is coiled.

(Kato et al., 2004)

Expression of dpp^Scer\UAS.cSa under the control of Scer\GAL4^twi.PG causes mislocalisation of crystal cells to more posterior positions in the embryo than normal.

(Milchanowski et al., 2004)

Testes of males expressing dpp^Scer\UAS.cSa under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} contain massive numbers of small early germ cells. The number of germ cells with a spectrosome dot (characteristic of gonialblasts) and of germ cells interconnected by small fusomes (characteristic of spermatogonial cysts) is increased compared to wild type. The testes contain large numbers of clusters of germ cells surrounded by somatic cyst cells. Clusters of more than 8 cells in a single cyst undergoing synchronous mitosis are often seen in the mutant testes (wild-type cysts never contain more than 8 cells undergoing synchronous division) as are cysts containing more than 16 spermatogonia interconnected by highly branched fusomes. The testes contain fewer spermatocytes than normal. Freshly eclosed males show signs of massive cell death in the more distal regions of the testes, while in testes of older males, often only dead or dying tissue is detected. Testes expressing dpp^Scer\UAS.cSa under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} show a statistically significant increase in the number of germline stem cells around the apical hub compared to wild type. The somatic hub is expanded compared to wild type in larval and adult testes of males expressing dpp^Scer\UAS.cSa under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} and in 38% of adult testes it is displaced away from the apical tip. In 6% of adults two somatic hubs are detected at different positions in the same testis.

(Schulz et al., 2004)

Expression of dpp^Scer\UAS.cSa under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} results in testes in which all germ cells resemble germ line stem cells and spermatogonia, and no spermatocytes or mature spermatids are present. The mutant testes contain a similar number of spectrosome-containing cells to wild-type testes, but the number of fusome-containing cells is much higher than wild type in the mutant testes (this suggests that the ectopic cells have spermatogonial identity). Cysts of germ cells in the mutant testis undergo synchronous mitotic division (another characteristic of spermatogonia), but the number of rounds of mitotic division exceeds the usual number of four. The mutant testes show significantly more cell death than wild-type testes.

(Shivdasani and Ingham, 2003)

Expression of dpp^Scer\UAS.cSa under the control of Scer\GAL4^byn-Gal4 does not affect formation of the hindgut boundary cell rows or the posterior boundary cell ring. The anterior boundary cell ring is missing in these embryos.

(Iwaki and Lengyel, 2002)

Expression of dpp^Scer\UAS.cSa under the control of Scer\GAL4^rho.PL causes dorsalisation of the ventral epidermis.

(Oh et al., 2002)

When expression is driven by Scer\GAL4^prd.RG1 or Scer\GAL4^en-e16E embryos are dorsalized.

(Arquier et al., 2001)

Clones in the eye disc expressing dpp^Scer\UAS.cSa under the control of Scer\GAL4^Act5C.PI only induce neural differentiation along the margin of the eye discs.

(Baonza and Freeman, 2001)

Expression of dpp^Scer\UAS.cSa under the control of Scer\GAL4^da.G32 results in embryos with a much wider amnioserosa and head epidermis than normal. Dorsolateral structures, such as the visual primordium, are relatively normal in size and shape, but are shifter to lateral or ventrolateral levels. Ventral tissues are partially missing. A high number of embryos heat pulsed between 3 and 5 hours post fertilisation (to express dpp^Scer\UAS.cSa under the control of Scer\GAL4^hs.PB) show a dorsalisation phenotype very similar to that caused by expression of dpp^Scer\UAS.cSa under the control of Scer\GAL4^da.G32. Later heat pulses have no effect on head patterning.

(Chang et al., 2001)

Expression of dpp^Scer\UAS.cSa in the follicle cells using Scer\GAL4^c532 at 29^oC results in eggs which completely lack respiratory appendages and have an enlarged operculum which extends over more than half the dorsal side of the eggshell. Expression of dpp^Scer\UAS.cSa in the follicle cells using Scer\GAL4^c532 at 18^oC results in eggs with an enlarged operculum surrounded by up to six respiratory appendages with significant variations in length and/or width.

(Dequier et al., 2001)

When dpp^Scer\UAS.cSa is driven by Scer\GAL4^GMR.PF or Scer\GAL4^Act5C.PP, a pronounced increase in the density of glial cell is seen in the entire optic system. In eye discs in which dpp^Scer\UAS.cSa is expressed under the control of Scer\GAL4^GMR.PF, the glial population migrates beyond its normal axonal boundary along the entire front. In milder cases, the glia migrate only a few rows beyond the normal anterior boundary of 2-4 rows posterior to the morphogenetic furrow. In a few cases they migrate even beyond the morphogenetic furrow. When clones of cells expressing dpp^Scer\UAS.cSa under the control of Scer\GAL4^Act5C.PP are made in the developing eye, ectopic differentiation of photoreceptors can sometimes be seen. In those eye discs where ectopic differentiation of photoreceptors is not seen, glial cells do not overshoot anteriorly, either in a broad front or a narrow stream. This mismigration is not preferentially directed toward the dpp^Scer\UAS.cSa clones. In those eye discs where ectopic differentiation of photoreceptors is seen, glial cells migrate specifically to such patches.

(Rangarajan et al., 2001)

Expression of dpp^Scer\UAS.cSa under the control of Scer\GAL4^ptc-559.1 results in excessive growth of the hindgut in embryos. Excessive growth of the hindgut is not seen when dpp^Scer\UAS.cSa is expressed under the control of Scer\GAL4^byn-Gal4 or Scer\GAL4^h-1J3.

(Takashima and Murakami, 2001)

Expression of dpp^Scer\UAS.cSa under the control of Scer\GAL4^btl.PS changes the identity of the presumptive dorsal trunk cells in the embryonic tracheal system and causes them to adhere to and migrate with the dorsal branch cells and form narrow diameter tubes like the dorsal branch.

(Beitel and Krasnow, 2000)

Eggs derived from females expressing dpp^Scer\UAS.cSa under the control of Scer\GAL4^hs.PB have an expanded operculum and reduced dorsal appendages. The eggshells are normal in other respects, and normal micropyles are formed at the extreme anterior of the eggshell. The mutant operculum generally has a normal organisation of large cell imprints surrounded by a raised "collar" structure. Expansion of the operculum always occurs over the dorsal side of the egg, indicating that dorsal-ventral patterning is unperturbed.

(Dobens et al., 2000)

Expression of dpp^Scer\UAS.cSa under the control of Scer\GAL4^30A results in a mirror image duplication of tissue at the anterior and overgrowth at the posterior in the wing.

(Haines and van Den Heuvel, 2000)

Expression of dpp^Scer\UAS.cSa under the control of Scer\GAL4^Dll-md23 increases the number of wing disc cells in the embryo.

(Kubota et al., 2000)

Ectopic expression of dpp^Scer\UAS.cSa under the control of Scer\GAL4^E4 in a wild-type background has no visible effect on chorion structure. Females expressing dpp^Scer\UAS.cSa under the control of Scer\GAL4^E4 and treated with colchicine produce eggs with dorsal appendage material posteriorly.

(Peri and Roth, 2000)

Expression of dpp^Scer\UAS.cSa under the control of Scer\GAL4^nos.PG results in embryos that lack ventral denticle belts.

(Tracey et al., 2000)

Embryos expressing dpp^Scer\UAS.cSa under the control of Scer\GAL4^69B do not show gross defects in dorsal closure. The embryos have poorly differentiated ventral cuticle. The ventral cuticle does not have dorsal hairs, indicating that it is not transformed to a dorsal fate. Wild-type patterns of asymmetric gastrulation are seen in these embryos.

(Byars et al., 1999)

Expression of dpp^Scer\UAS.cSa under the control of Scer\GAL4^30A does not result in the induction of ectopic retinal cells in the wing disc.

(Chen et al., 1999)

When expression is driven by Scer\GAL4^arm.PS the spiracular chamber does not invaginate, though all the structures of the spiracular chamber (trachea link, filzkorper, spiracular hairs) differentiate, as determined by cell shape markers.

(Hu and Castelli-Gair, 1999)

Expression of dpp^Scer\UAS.cSa under the control of Scer\GAL4^vg.int2.1 leads to an enlargement of the wing blade along the anterior/posterior axis in the wing disc.

(Klein and Martinez Arias, 1999)

When expression is driven by Scer\GAL4^T155 a large lateral region of the tergite is transformed to pleural identity, while the sternite is reduced to approximately 25% of its normal size. The number of bristles remains unchanged. The piment band of the tergite is broadened. No stronger effect can be caused by increasing the dosage of dpp^Scer\UAS.cSa. A similar but weaker phenotype is seen when expression is driven by Scer\GAL4^dpp.blk1. Clones induced in the sternite or tergite, driven by Scer\GAL4^αTub84B.PP, cause nonautonomous transformations.

(Kopp et al., 1999)

Wing discs expressing dpp^Scer\UAS.cSa under the control of Scer\GAL4^30A grow abnormally large.

(Minami et al., 1999)

Expression driven ubiquitously with Scer\GAL4^hs.PB causes an inhibition of S phase in the eye disc and the antennal disc.

(Horsfield et al., 1998)

Neither Scer\GAL4^C-765- nor Scer\GAL4^dpp.blk1-mediated expression induces leg to wing transdetermination.

(Maves and Schubiger, 1998)

Scer\GAL4^zen.Kr.PF-mediated expression represses fat body development in parasegments 6-8. Scer\GAL4^twi.PGa-mediated expression represses fat body development in parasegments 4-12 but promotes it in parasegment 13 resulting in ectopic fat body precursors in the ventral mesoderm. These cells lie in the mesoderm above the invaginating posterior midgut.

(Riechmann et al., 1998)

When expressed under the influence of Scer\GAL4^how-24B, midgut constrictions are affected.

(Szuts et al., 1998)

Embryos expressing dpp^Scer\UAS.cSa under the control of Scer\GAL4^how-24B lack the first and third midgut constrictions, and have an abnormally deep second midgut constriction.

(Wisotzkey et al., 1998)

Scer\GAL4^c532-mediated expression generates eggs with more posteriorly located and malformed dorsal appendages. Sometimes more than two chorionic appendages are produced in one egg.

(Deng and Bownes, 1997)

Scer\GAL4-mediated lethality occurs during larval and pupal periods. Scer\GAL4^E132-mediated expression causes the development of ectopic morphogenetic furrows (MF). Most ectopic MFs initiated at the anterior edge of the eye disc and propogated posteriorly, few initiate from the ventral margin and propogate dorsally. In some discs the ommatidial array extends into regions of that disc that normally give rise to the head cuticle surrounding the compound eye. The normal and ectopic eye fields fuse forming a lawn of neuronal clusters covering essentially the whole disc. dpp induced clones in the eye disc (expressed from Scer\GAL4^Act5C.PP) cause ectopic morphogenetic furrows that propogate posteriorly from the anterior margin. Overgrowth of the anterior eye disc occurs frequently and in some cases a new eye disc is generated. Study of the clone phenotypes confirms that specific regions of the eye are competent to respond to dpp and ectopic dpp exercises its effect at a considerable distance.

(Pignoni and Zipursky, 1997)

Scer\GAL4^btl.PS-mediated expression diverts cells of the dorsal trunk and visceral branch primordia to the dorsal branch.

(Wappner et al., 1997)

Produces ectopic vein tissue in the wing when expressed using Scer\GAL4^C-580 at 17^oC or 25^oC.

(de Celis, 1997)

When expression is driven by Scer\GAL4^hs.PB, embryos show shifts in the dorsal fate map that are less extreme than those caused by Scer\GAL4^ptc-559.1. However there are no changes in dorsal pattern across the segment, the 1^o, 2^o, 3^o and 4^o cell types are all present in either case.

(Bokor and DiNardo, 1996)

When expressed from Scer\GAL4^dpp.blk1, wing discs are greatly expanded along the anteroposterior axis of the wing blade region, though the dorsal, notal region remains unaffected.

(Burke and Basler, 1996)

Scer\GAL4^dpp.blk1 induced overexpression of dpp induces foreleg bifurcations and foreleg fusions in a dose-dependent manner. Intermediate increase in dpp expression causes supernumerary bifurcations to emerge from the anterior-ventral side of the leg that consist of anterior-ventral leg structures. Higher levels of dpp expression cause fused proximal segments and foreleg pairs become fused along the entire proximal-distal axis. The second and third leg disc pairs exhibit a low frequency of incomplete ventral bifurcations. An intermediate increase in dpp expression has little effect on wing and thorax patterning, except for thickening of the anterior cross vein. Higher levels of dpp expression causes defects in the scutellum. At the highest temperature flies are recovered at pharate adults with the most severely reduced scutellum have wing duplications. The duplications arise from the posterior hinge region and posteior wing blade structures.

(Morimura et al., 1996)

When expressed with Scer\GAL4^69B ventral cells are dorsalised. Expression with Scer\GAL4^C-765 results in large overproliferating discs.

(Nellen et al., 1996)

Failure of all of the midgut morphogenetic events except formation of constriction 2.

(Staehling-Hampton and Hoffmann, 1994)

Expression induced by Scer\GAL4^69B causes the ventral denticle belts to be replaced by fine hairs characteristic of the dorsal surface.

(Staehling-Hampton et al., 1994)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^{VP16.nanos.UTR}, dpp^UAS.cSa has abnormal cell migration phenotype, suppressible | partially by Scer\GAL4^{VP16.nanos.UTR}/lwr^UAS.cAa

(Deshpande et al., 2014)

Scer\GAL4^30A, dpp^UAS.cSa has visible phenotype, suppressible by Su(hh)VII^VII

(Haines and van Den Heuvel, 2000)

Scer\GAL4^30A, dpp^UAS.cSa has visible phenotype, suppressible | partially by Su(hh)II^II

(Haines and van Den Heuvel, 2000)

Scer\GAL4^30A, dpp^UAS.cSa has visible phenotype, suppressible | partially by Su(hh)IV^IV

(Haines and van Den Heuvel, 2000)

Scer\GAL4^30A, dpp^UAS.cSa has visible phenotype, suppressible | partially by Su(hh)I^I

(Haines and van Den Heuvel, 2000)

NOT suppressed by

Statement

Reference

Scer\GAL4^30A, dpp^UAS.cSa has visible phenotype, non-suppressible by Su(hh)III^III

(Haines and van Den Heuvel, 2000)

Scer\GAL4^30A, dpp^UAS.cSa has visible phenotype, non-suppressible by Su(hh)V^V

(Haines and van Den Heuvel, 2000)

Scer\GAL4^30A, dpp^UAS.cSa has visible phenotype, non-suppressible by Su(hh)VI^VI

(Haines and van Den Heuvel, 2000)

Enhancer of

Statement

Reference

dpp^UAS.cSa/wg^UAS.cLa, Scer\GAL4^ap-md544 is an enhancer of hyperplasia phenotype of Egfr^UAS.cBa, Scer\GAL4^ap-md544

(Herranz et al., 2014)

NOT Enhancer of

Statement

Reference

dpp^UAS.cSa, Scer\GAL4^ap-md544 is a non-enhancer of hyperplasia phenotype of Egfr^UAS.cBa, Scer\GAL4^ap-md544

(Herranz et al., 2014)

NOT Suppressor of

Statement

Reference

dpp^UAS.cSa/Scer\GAL4^hh.PU is a non-suppressor of increased cell death | larval stage phenotype of Scer\GAL4^hh.PU, Spyo\Cas9^UAS.P2, wg^{UAS.wg+intergenic-1.pCFD6}

(Ewen-Campen and Perrimon, 2024)

Other

Statement

Reference

Egfr^UAS.cBa, Scer\GAL4^ap-md544, dpp^UAS.cSa, wg^UAS.cLa has neoplasia phenotype

(Herranz et al., 2014)

Scer\GAL4^bi-omb-Gal4, dpp^UAS.cSa, ey^UAS.cHa, hh^UAS.cAa has lethal | larval stage phenotype

(Kango-Singh et al., 2003)

Egfr^DN.UAS, Scer\GAL4^T155, dpp^UAS.cSa has visible phenotype

(Kopp et al., 1999)

Scer\GAL4^T155, dpp^UAS.cSa, wg^UAS.cLa has visible phenotype

(Kopp et al., 1999)

Egfr^DN.UAS, Scer\GAL4^T155, dpp^UAS.cSa has abnormal size phenotype

(Kopp et al., 1999)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^30A, dpp^UAS.cSa, ey^UAS.cHa, eya^UAS.cPa has wing disc phenotype, enhanceable by so^UAS.cPa/Scer\GAL4^30A

(Pappu et al., 2003)

Scer\GAL4^30A, dpp^UAS.cSa, ey^UAS.cHa, eya^UAS.cPa has photoreceptor neuron | ectopic phenotype, enhanceable by so^UAS.cPa/Scer\GAL4^30A

(Pappu et al., 2003)

Suppressed by

Statement

Reference

Scer\GAL4^{VP16.nanos.UTR}, dpp^UAS.cSa has germline cell phenotype, suppressible | partially by Scer\GAL4^{VP16.nanos.UTR}/lwr^UAS.cAa

(Deshpande et al., 2014)

Scer\GAL4^{VP16.nanos.UTR}, dpp^UAS.cSa has gonad phenotype, suppressible | partially by Scer\GAL4^{VP16.nanos.UTR}/lwr^UAS.cAa

(Deshpande et al., 2014)

Scer\GAL4^en-e16E, dpp^UAS.cSa has embryonic epidermis phenotype, suppressible by l(2)gl^ts3

(Arquier et al., 2001)

Scer\GAL4^prd.RG1, dpp^UAS.cSa has embryonic epidermis phenotype, suppressible by l(2)gl^ts3

(Arquier et al., 2001)

Scer\GAL4^30A, dpp^UAS.cSa has wing phenotype, suppressible by Su(hh)VII^VII

(Haines and van Den Heuvel, 2000)

Scer\GAL4^30A, dpp^UAS.cSa has wing phenotype, suppressible | partially by Su(hh)I^I

(Haines and van Den Heuvel, 2000)

Scer\GAL4^30A, dpp^UAS.cSa has wing phenotype, suppressible | partially by Su(hh)II^II

(Haines and van Den Heuvel, 2000)

Scer\GAL4^30A, dpp^UAS.cSa has wing phenotype, suppressible | partially by Su(hh)IV^IV

(Haines and van Den Heuvel, 2000)

Scer\GAL4^how-24B, dpp^UAS.cSa has embryonic midgut constriction phenotype, suppressible | partially by Egfr^DN.UAS, Scer\GAL4^how-24B

(Szuts et al., 1998)

Scer\GAL4^how-24B, dpp^UAS.cSa has embryonic midgut constriction 1 phenotype, suppressible by Med⁴/Med²

(Wisotzkey et al., 1998)

Scer\GAL4^how-24B, dpp^UAS.cSa has embryonic midgut constriction 3 phenotype, suppressible by Med⁴/Med²

(Wisotzkey et al., 1998)

NOT suppressed by

Statement

Reference

Scer\GAL4^30A, dpp^UAS.cSa has wing phenotype, non-suppressible by Su(hh)III^III

(Haines and van Den Heuvel, 2000)

Scer\GAL4^30A, dpp^UAS.cSa has wing phenotype, non-suppressible by Su(hh)V^V

(Haines and van Den Heuvel, 2000)

Scer\GAL4^30A, dpp^UAS.cSa has wing phenotype, non-suppressible by Su(hh)VI^VI

(Haines and van Den Heuvel, 2000)

Enhancer of

Statement

Reference

dpp^UAS.cSa/Scer\GAL4^ap-md544 is an enhancer of wing disc phenotype of Scer\GAL4^ap-md544, ey^UAS.cHa

(Nfonsam et al., 2012)

dpp^UAS.cSa/Scer\GAL4^ap-md544 is an enhancer of ommatidium | ectopic phenotype of Scer\GAL4^ap-md544, ey^UAS.cHa

(Nfonsam et al., 2012)

dpp^UAS.cSa/Scer\GAL4^Act5C.PI is an enhancer of wing disc | somatic clone phenotype of Scer\GAL4^Act5C.PI, ey^UAS.cHa, hh^UAS.cAa

(Kango-Singh et al., 2003)

dpp^UAS.cSa/Scer\GAL4^30A is an enhancer of wing disc phenotype of Scer\GAL4^30A, ey^UAS.cHa

(Chen et al., 1999)

dpp^UAS.cSa/Scer\GAL4^hs.PB is an enhancer of eye phenotype of CycE^JP

(Horsfield et al., 1998)

NOT Enhancer of

Statement

Reference

dpp^UAS.cSa/Scer\GAL4^ey.PB is a non-enhancer of eye disc | somatic clone phenotype of smo^D16

(Pappu et al., 2003)

dpp^UAS.cSa/Scer\GAL4^ey.PB is a non-enhancer of eye disc morphogenetic furrow | somatic clone phenotype of smo^D16

(Pappu et al., 2003)

dpp^UAS.cSa/Scer\GAL4^ey.PB is a non-enhancer of photoreceptor neuron | somatic clone phenotype of smo^D16

(Pappu et al., 2003)

dpp^UAS.cSa, Scer\GAL4^ey.PB, so^UAS.cPa is a non-enhancer of eye disc | somatic clone phenotype of smo^D16

(Pappu et al., 2003)

dpp^UAS.cSa, Scer\GAL4^ey.PB, so^UAS.cPa is a non-enhancer of photoreceptor neuron | somatic clone phenotype of smo^D16

(Pappu et al., 2003)

Suppressor of

Statement

Reference

dpp^UAS.cSa/Scer\GAL4^{kn-col85-GAL4} is a suppressor | partially of lamellocyte | larval stage phenotype of Scer\GAL4^{kn-col85-GAL4}, heca^GD8578

(Varga et al., 2019)

dpp^UAS.cSa/Scer\GAL4^{VP16.nanos.UTR} is a suppressor | partially of germline cell | female | larval stage phenotype of nanos¹⁸/Df(3R)Delta-FX3

(Gilboa and Lehmann, 2004)

dpp^UAS.cSa/Scer\GAL4^{VP16.nanos.UTR} is a suppressor | partially of germline cyst | ectopic phenotype of nanos¹⁸/Df(3R)Delta-FX3

(Gilboa and Lehmann, 2004)

dpp^UAS.cSa, Scer\GAL4^ey.PB, eya^UAS.cPa is a suppressor of photoreceptor neuron | somatic clone phenotype of smo^D16

(Pappu et al., 2003)

dpp^UAS.cSa, Scer\GAL4^ey.PB, eya^UAS.cPa is a suppressor of eye disc morphogenetic furrow | somatic clone phenotype of smo^D16

(Pappu et al., 2003)

dpp^UAS.cSa/Scer\GAL4^Dll-md23 is a suppressor of leg disc phenotype of Scer\GAL4^Dll-md23, spi^s.UAS

(Kubota et al., 2000)

dpp^UAS.cSa/Scer\GAL4^Dll-md23 is a suppressor | partially of wing disc phenotype of Scer\GAL4^Dll-md23, spi^s.UAS

(Kubota et al., 2000)

dpp^UAS.cSa/Scer\GAL4^en-e16E is a suppressor | partially of larval hindgut & embryo & cell phenotype of D³/Df(3L)fz-GS1a

(Sanchez Soriano and Russell, 2000)

dpp^UAS.cSa/Scer\GAL4^C-580 is a suppressor of wing vein phenotype of rho^ve-1, vn¹

(de Celis, 1997)

NOT Suppressor of

Statement

Reference

dpp^UAS.cSa/Scer\GAL4^hh.PU is a non-suppressor of wing disc posterior compartment | larval stage phenotype of Scer\GAL4^hh.PU, Spyo\Cas9^UAS.P2, wg^{UAS.wg+intergenic-1.pCFD6}

(Ewen-Campen and Perrimon, 2024)

dpp^UAS.cSa, Scer\GAL4^ey.PB, so^UAS.cPa is a non-suppressor of eye disc | somatic clone phenotype of smo^D16

(Pappu et al., 2003)

dpp^UAS.cSa, Scer\GAL4^ey.PB, so^UAS.cPa is a non-suppressor of photoreceptor neuron | somatic clone phenotype of smo^D16

(Pappu et al., 2003)

dpp^UAS.cSa/Scer\GAL4^ey.PB is a non-suppressor of eye disc | somatic clone phenotype of smo^D16

(Pappu et al., 2003)

dpp^UAS.cSa/Scer\GAL4^ey.PB is a non-suppressor of eye disc morphogenetic furrow | somatic clone phenotype of smo^D16

(Pappu et al., 2003)

dpp^UAS.cSa/Scer\GAL4^ey.PB is a non-suppressor of photoreceptor neuron | somatic clone phenotype of smo^D16

(Pappu et al., 2003)

dpp^UAS.cSa/Scer\GAL4^dpp.blk1 is a non-suppressor of eye disc morphogenetic furrow phenotype of Scer\GAL4^dpp.blk1, wg^wg.3'UTR.UAS

(Cadigan et al., 2002)

Other

Statement

Reference

Scer\GAL4^CY2, dpp^UAS.cSa, grk^2B6/grk^HF has egg chorion phenotype

(Shravage et al., 2007)

Scer\GAL4^CY2, dpp^UAS.cSa, grk^2B6/grk^HF has egg phenotype

(Shravage et al., 2007)

Scer\GAL4^CY2, dpp^UAS.cSa, grk^2B6/grk^HF has micropyle phenotype

(Shravage et al., 2007)

Scer\GAL4^GR1, Scer\GAL4^{VP16.mat.αTub67C}, dpp^UAS.cSa, grk^UAS.cBa has dorsal appendage phenotype

(Shravage et al., 2007)

Scer\GAL4^GR1, Scer\GAL4^{VP16.mat.αTub67C}, dpp^UAS.cSa, grk^UAS.cBa has egg operculum phenotype

(Shravage et al., 2007)

Scer\GAL4^CY2, Scer\GAL4^{VP16.mat.αTub67C}, dpp^UAS.cSa, grk^UAS.cBa has dorsal appendage phenotype

(Shravage et al., 2007)

Scer\GAL4^CY2, Scer\GAL4^{VP16.mat.αTub67C}, dpp^UAS.cSa, grk^UAS.cBa has egg operculum phenotype

(Shravage et al., 2007)

Scer\GAL4^Act5C.PI, dpp^UAS.cSa, ey^UAS.cHa, hh^UAS.cAa has wing disc | ectopic | somatic clone phenotype

(Kango-Singh et al., 2003)

Scer\GAL4^30A, dpp^UAS.cSa, ey^UAS.cHa, eya^UAS.cPa has photoreceptor neuron | ectopic phenotype

(Pappu et al., 2003)

Scer\GAL4^30A, dpp^UAS.cSa, ey^UAS.cHa, hh^UAS.cCa has wing disc phenotype

(Pappu et al., 2003)

Scer\GAL4^30A, dpp^UAS.cSa, ey^UAS.cHa, hh^UAS.cCa has photoreceptor neuron | ectopic phenotype

(Pappu et al., 2003)

Scer\GAL4^30A, dpp^UAS.cSa, ey^UAS.cHa has photoreceptor neuron | ectopic phenotype

(Pappu et al., 2003)

Scer\GAL4^30A, dpp^UAS.cSa, ey^UAS.cHa has wing disc | posterior phenotype

(Pappu et al., 2003)

Scer\GAL4^30A, dpp^UAS.cSa, ey^UAS.cHa, eya^UAS.cPa has wing disc phenotype

(Pappu et al., 2003)

Delta^UAS.cJa, Scer\GAL4^Act5C.PI, dpp^UAS.cSa has eye disc | somatic clone phenotype

(Baonza and Freeman, 2001)

Scer\GAL4^vg.int2.1, dpp^UAS.cSa, wg^UAS.cGa has wing disc phenotype

(Klein and Martinez Arias, 1999)

Egfr^DN.UAS, Scer\GAL4^T155, dpp^UAS.cSa has abdominal tergite phenotype

(Kopp et al., 1999)

Scer\GAL4^T155, dpp^UAS.cSa, wg^UAS.cLa has abdominal tergite phenotype

(Kopp et al., 1999)

Scer\GAL4^T155, dpp^UAS.cSa, wg^UAS.cLa has abdominal sternite phenotype

(Kopp et al., 1999)

Scer\GAL4^T155, dpp^UAS.cSa, wg^UAS.cLa has pleurum phenotype

(Kopp et al., 1999)

Scer\GAL4^dpp.blk1, dpp^UAS.cSa, wg^Act5C.PS has mesothoracic leg phenotype

(Maves and Schubiger, 1998)

Scer\GAL4^dpp.blk1, dpp^UAS.cSa, wg^Act5C.PS has mesothoracic coxa phenotype

(Maves and Schubiger, 1998)

Scer\GAL4^dpp.blk1, dpp^UAS.cSa, wg^Act5C.PS has mesothoracic trochanter phenotype

(Maves and Schubiger, 1998)

Scer\GAL4^dpp.blk1, dpp^UAS.cSa, wg^Act5C.PS has mesothoracic femur phenotype

(Maves and Schubiger, 1998)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of lwr^Scer\UAS.cAa with dpp^Scer\UAS.cSa in primordial germ cells, under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} does not restore the number of primoridal germ cells to wild-type, but does alter the distribution of these cells to a more wild-type manner.

(Deshpande et al., 2014)

Expression of either wg^Scer\UAS.cLa or dpp^Scer\UAS.cSa does not enhance the overproliferation seen in wing discs expressing Egfr^Scer\UAS.cBa under the control of Scer\GAL4^ap-md544. However, co-expression of wg^Scer\UAS.cLa and dpp^Scer\UAS.cSa with Egfr^Scer\UAS.cBa produces tumors consisting of epithelial and mesenchymal cell populations and showing loss of epithelial integrity.

(Herranz et al., 2014)

Co-expression of dpp^Scer\UAS.cSa results in enlarged wing discs containing a larger field (30-40% of disc surface area) of differentiating ectopic eye precursors compared to expression of ey^Scer\UAS.cHa alone under the control of Scer\GAL4^ap-md544.

(Nfonsam et al., 2012)

Eggs derived from females expressing dpp^Scer\UAS.cSa under the control of Scer\GAL4^CY2 in a grk^2B6/grk^HF background have misshaped micropyles, show the loss of the collar that normally separates the operculum from the rest of the chorion and show a reduction in egg diameter.

Co-expression of dpp^Scer\UAS.cSa and grk^Scer\UAS.cBa under the control of Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} and Scer\GAL4^GR1 results in eggs with a large anterior operculum followed by a symmetrical band of dorsal appendage material.

Co-expression of dpp^Scer\UAS.cSa and grk^Scer\UAS.cBa under the control of Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} and Scer\GAL4^CY2 results in eggs with an expansion of the operculum at the expense of dorsal appendage material.

(Shravage et al., 2007)

The precocious differentiation of primordial germ cells which is seen in nos¹⁸/Df(3R)Dl-FX3 larval ovaries is partially suppressed by expression of dpp^Scer\UAS.cSa under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16}.

(Gilboa and Lehmann, 2004)

When dpp^Scer\UAS.cSa, hh^Scer\UAS.cAa and ey^Scer\UAS.cHa are co-expressed in clones (driven by Scer\GAL4^Act5C.PI), very strong overgrowth and distorted morphology is seen in the wing disc. Co-expression of dpp^Scer\UAS.cSa, hh^Scer\UAS.cAa and ey^Scer\UAS.cHa driven by Scer\GAL4^bi-omb-Gal4, causes a high larval lethality at 25^oC.

(Kango-Singh et al., 2003)

When dpp^Scer\UAS.cSa and ey^Scer\UAS.cHa are driven by Scer\GAL4^30A, photoreceptor differentiation is induced in the wing disc, only in the posterior compartment. When dpp^Scer\UAS.cSa, ey^Scer\UAS.cHa and hh^Scer\UAS.cCa are driven by Scer\GAL4^30A, photoreceptor differentiation is induced in both compartments of the wing disc. When dpp^Scer\UAS.cSa, eya^Scer\UAS.cPa and ey^Scer\UAS.cHa are driven by Scer\GAL4^30A, photoreceptor differentiation is induced in both compartments of the wing disc. The addition of so^Scer\UAS.cPa increases the penetrance of this phenotype. The addition of dpp^Scer\UAS.cSa or eya^Scer\UAS.cPa (driven by Scer\GAL4^ey.PB) to animals with smo^D16 clones in the eye disc has no effect on the photoreceptor differentiation phenotype seen in eye discs. The delayed progression of the morphogenetic furrow is also not affected. The addition of dpp^Scer\UAS.cSa and eya^Scer\UAS.cPa (driven by Scer\GAL4^ey.PB) to animals with smo^D16 clones in the eye disc, fully suppresses photoreceptor differentiation phenotype seen in eye discs. The delayed progression of the morphogenetic furrow is also rescued. The addition of dpp^Scer\UAS.cSa and eya^Scer\UAS.cPa (driven by Scer\GAL4^ey.PB) to animals with smo^D16 clones in the eye disc, fully suppresses photoreceptor differentiation phenotype seen in eye discs. The delayed progression of the morphogenetic furrow is also rescued. If so^Scer\UAS.cPa is also added, posterior margin clones are still rescued, although ectopic anterior furrows are induced with high frequency. The addition of so^Scer\UAS.cPa and dpp^Scer\UAS.cSa (driven by Scer\GAL4^ey.PB) to animals with smo^D16 clones in the eye disc has no effect on the photoreceptor differentiation phenotype seen in eye discs.

(Pappu et al., 2003)

Co-expression of dpp^Scer\UAS.cSa does not rescue progression of the morphogenetic furrow in animals expressing wg^{wg.3'UTR.Scer\UAS} under the control of Scer\GAL4^dpp.blk1.

(Cadigan et al., 2002)

Clones in the eye disc expressing both dpp^Scer\UAS.cSa and Dl^Scer\UAS.cJa under the control of Scer\GAL4^Act5C.PI result in neural differentiation in all regions anterior to the morphogenetic furrow. Neural differentiation is induced in all the cells surrounding the clone.

(Baonza and Freeman, 2001)

The wing phenotype caused by expression of dpp^Scer\UAS.cSa under the control of Scer\GAL4^30A is mildly suppressed by Su(hh)I^I, Su(hh)II^II or Su(hh)IV^IV, strongly suppressed by Su(hh)VII^VII and not suppressed by Su(hh)III^III, Su(hh)V^V or Su(hh)VI^VI.

(Haines and van Den Heuvel, 2000)

When dpp^Scer\UAS.cSa is expressed under the control of Scer\GAL4^en-e16E in D³/Df(3L)fz-GS1a embryos, significant, though variable rescue of the D³/Df(3L)fz-GS1a hindgut phenotype is seen.

(Sanchez Soriano and Russell, 2000)

Enhances the ectopic retinal development phenotype in the wing and haltere discs caused by expression of ey^Scer\UAS.cHa under the control of Scer\GAL4^30A.

(Chen et al., 1999)

Coexpression of dpp^Scer\UAS.cSa and wg^Scer\UAS.cGa under the control of Scer\GAL4^vg.int2.1 leads to a pronounced extension of the wing blade towards the notum in the wing disc. In some cases, the development of up to 8 wing blades, all with a common origin over a point ventral to the notum, is seen.

(Klein and Martinez Arias, 1999)

When dpp^Scer\UAS.cSa and wg^Scer\UAS.cLa are coexpressed via Scer\GAL4^T155 in pupae, abdominal pattern is restored to wild type, indicating that pleural and tergite/sternite fates are specified by the balance between competing wg and dpp activities. When co-expression of Egfr^DN.Scer\UAS and dpp^Scer\UAS.cSa is driven by Scer\GAL4^T155 the tergite is reduced to a very small patch near the dorsal midline and the lateral tergite is completely transformed to pleura. These effects are not due to reduced histoblast proliferation.

(Kopp et al., 1999)

wg^Act5C.PS; Scer\GAL4^dpp.blk1/dpp^Scer\UAS.cSa flies die before adulthood, transplanted second leg discs exhibit wg-induced transdetermination, legs with wing tissue. Coxa, trochanter and femur segments are reduced. Ventral wing hinge structures are observed at opposite regions within the mesothoracic leg tissue.

(Maves and Schubiger, 1998)

dpp^Scer\UAS.cSa under the influence of Scer\GAL4^how-24B, gut morphology is more normal than for dpp^Scer\UAS.cSa, Scer\GAL4^how-24B mutants alone.

When Egfr^DN.Scer\UAS is expressed in combination with dpp^Scer\UAS.cSa under the influence of Scer\GAL4^how-24B, gut morphology is more normal than for dpp^Scer\UAS.cSa, Scer\GAL4^how-24B mutants alone.

(Szuts et al., 1998)

The first midgut constriction is restored in more than 90% of cases of embryos expressing dpp^Scer\UAS.cSa under the control of Scer\GAL4^how-24B if the embryo also carries Med²/Med⁴. In all embryos where the first constriction is restored, there is also a significant rescue of the third midgut constriction.

(Wisotzkey et al., 1998)

vn¹ rho^ve-1 double homozygotes lack all longitudinal wing veins. Vein tissue differentiates in vn¹ rho^ve-1 double homozygotes when dpp^Scer\UAS.cSa is expressed in these flies using Scer\GAL4^C-580.

(de Celis, 1997)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

dpp^UAS.cSa/Scer\GAL4^dpp.blk1 rescues dpp^d-blk

(Staehling-Hampton et al., 1994)

dpp^UAS.cSa/Scer\GAL4^dpp.blk1 rescues dpp^d6

(Staehling-Hampton et al., 1994)

Comments

Expression of Scer\GAL4^dpp.blk1 in a dpp^d-blk background rescues the mutant eye phenotype, restoring the number of ommatidia to approximately that of wild type eyes and in a dpp^d6 background rescues the mutant eye and wing phenotypes.

(Staehling-Hampton et al., 1994)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

1486

y¹ w^*; P{UAS-dpp.S}42B.4

Kyoto

106343

w^*; P{UAS-dpp.S}42B.4

Notes on Origin