Allele Dmel\ftz-f1^hs.PW

General Information
Symbol	Dmel\ftz-f1^hs.PW	Species	D. melanogaster
Name	heat shock construct of Woodard	FlyBase ID	FBal0034690
Feature type	allele	Associated gene	Dmel\ftz-f1

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Woodard et al., 1994)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Expression of ftz-f1 driven by an Hsp70 promoter. (Woodard et al., 1994)
Carried in construct	P{F-F1} (Lee et al., 2002, Woodard et al., 1994, Broadus et al., 1999)
Cytology
Phenotypic Data
Phenotypic Class
	increased cell death \| heat sensitive (Lee et al., 2002)
Phenotype Manifest In
	salivary gland \| heat sensitive (Lee et al., 2002)
Detailed Description
	Statement Reference The salivary glands of mutant pupae heatshocked at 37^oC and aged to 6 hours APF, do not have salivary glands, and the degraded remnants exhibit signs of DNA fragmentation and thus cell death. (Lee et al., 2002)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement Reference ftz-f1^hs.PW has increased cell death \| heat sensitive phenotype, suppressible by Eip93F¹/Df(3R)93F^x2 (Lee et al., 2002)
Phenotype Manifest In
Suppressed by
Statement Reference ftz-f1^hs.PW has salivary gland \| heat sensitive phenotype, suppressible \| partially by Eip93F¹/Df(3R)93F^x2 (Lee et al., 2002)
Additional Comments
Genetic Interactions
Statement Reference The addition of Eip93F¹/Df(3R)93F^x2 to heat shocked ftz-f1^hs.PW animals leads to a reduction in the cell death (and thus intact salivary glands) seen in salivary glands though DNA fragmentation is still seen. (Lee et al., 2002)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Partially rescues	ftz-f1^hs.PW partially rescues ftz-f1¹⁷ (Broadus et al., 1999)
Comments	Heat shock of ftz-f1^hs.PW applied at 29^oC after 2.5 hours of prepupal development at 25^oC rescues the lethality of ftz-f1¹⁷ to 60%. (Broadus et al., 1999)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	ftz-f1^hs.PW
Name Synonym	heat shock construct of Woodard (Woodard et al., 1994)
Secondary FlyBase IDs

References ( 3 )
Research paper	Lee et al., 2002, Dev. Biol. 252(1): 138--148 Genetic mechanism for the stage- and tissue-specific regulation of steroid triggered programmed cell death in Drosophila. [FBrf0155496] Broadus et al., 1999, Mol. Cell 3(2): 143--149 The Drosophila beta FTZ-F1 orphan nuclear receptor provides competence for stage-specific responses to the steroid hormone ecdysone. [FBrf0107624] Woodard et al., 1994, Cell 79(4): 607--615 A molecular mechanism for the stage specificity of the Drosophila prepupal genetic response to ecdysone. [FBrf0076042]

Allele Dmel\ftz-f1hs.PW

Allele Dmel\ftz-f1^hs.PW