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General Information
Symbol
Dmel\Cul306430
Species
D. melanogaster
Name
FlyBase ID
FBal0034977
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
gftPZ06430, gft6430, cul3gft06430, gft06430
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

P{PZ} insertion in the 5' UTR, 228bp from exon 2.

P{PZ} insertion in the first intron of gft, 228bp from exon 2.

Insertion components
P{PZ}Cul306430
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Cul306430 mutant clones in sensory neurons in adult wing do not display any defects in injury-induced axon degeneration (following an axotomy, the severed axons are cleared away normally).

Homozygous and gft06430/Df(2L)TE35D-15 animals hatch, but die during the second larval instar. Females carrying homozygous germ-line clones produce few eggs and those which are laid are about half the size of wild-type and have fused dorsal appendages. No discernible embryonic structure can be detected interior to the chorion in these eggs. Large homozygous clones in the wing show a number of defects, including defects in the overall shape of the wing and in formation and position of the wing veins. Large clones in the wing often lack trichomes in the centre of the clone. Clones that cover the L3 vein often result in it shifting posteriorly. Clones that cover wing veins are associated with loss of vein tissue. Clones in the wing also result in ectopic sensory organ formation; clones that encompass a large part of the L3 vein have ectopic campaniform sensilla, ectopic campaniform sensilla also arise between wing vein L2 and the anterior wing margin and ectopic bristles arise distally between L2 and L3. Clones in the notum are associated with significant tufting of both micro- and macrochaetae, with the tufts being made up of ectopic fully formed external sensory organs as well as individual sensory organs that contain multiple shafts within a single socket. Homozygous clones in the wing disc show a dramatic increase in the number of sensory organ precursor (SOP) cells, while SOP development outside the clone is normal.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Statement
Reference

gft[+]/Cul306430 is a suppressor of visible phenotype of GαsQ215L.UAS, Scer\GAL4OK10

NOT Suppressor of
Phenotype Manifest In
Suppressor of
Statement
Reference

Cul3[+]/Cul306430 is a suppressor | partially of eye phenotype of Scer\GAL4GMR.PS, klhl10UAS.cKa

Cul306430/Cul-3[+] is a suppressor of eye phenotype of DrefUAS.cSa

Cul306430/Cul-3[+] is a suppressor | partially of wing blade | heat sensitive phenotype of Cul5EP3390, Scer\GAL4sd-SG29.1

gft[+]/Cul306430 is a suppressor of wing phenotype of GαsQ215L.UAS, Scer\GAL4OK10

NOT Suppressor of
Additional Comments
Genetic Interactions
Statement
Reference

The axon degeneration in sensory neurons clones in the adult wing expressing Ect4ΔARM.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4VGlut-OK371 can be suppressed by combination with Cul306430.

Cul306430/+ partially suppresses the reduced eye phenotype of flies expressing klhl10Scer\UAS.cKa under the control of Scer\GAL4GMR.PS.

The wing blade defects observed in animals expressing Cul-5EP3390 in the wing and haltere disc under the control of Scer\GAL4sd-SG29.1 is partially rescued in a Cul-306430/+ mutant background.

gft06430/+ moderately suppresses the phenotype caused by expression of G-sα60AQ215L.Scer\UAS under the control of Scer\GAL4OK10; wing blistering is reduced.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer

A. Spradling.

Comments
Comments

Precise excision of the insertion reverts the ability of gft06430 to modify the phenotype caused by expression of G-sα60AQ215L.Scer\UAS under the control of Scer\GAL4OK10.

Complements: CycE05206. Complements: wek05271a. Complements: CycE05277. Complements: CycE05278. Complements: CG1526905441. Complements: esg05730. Complements: esg07082. Complements: l(2)35Bd10408. Complements: CycE10427.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (15)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (21)