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General Information
Symbol
Dmel\ftk07918
Species
D. melanogaster
Name
FlyBase ID
FBal0035084
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
ft18, l(2)k07918k07918
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
Homozygous clones in the retina show inversions and misorientations of ommatidia with cell non-autonomous defects at the border of the clone.
ftk07918 mutants show planar cell polarity defects in the wings that extend distal to the posterior crossvein. The wings are foreshortened along the proximodistal axis of the wing blade as the anterior and posterior crossveins are abnormally close.
When ftk07918 somatic clones are made in the wing, clones contain significantly more cells than wild-type clones. These clones grow preferentially in the proximal part of the wing. When ftk07918 mutant wing and eye imaginal discs are made with clones, an overgrowth phenotype is seen.
The average number of crystal cells per embryo is reduced (to 16) in homozygous stage 13-14 embryos compared to wild type (36 +/- 2.2 cells on average).
Homozygous ftk07918 larvae show a small delay (1-2 days) to pupariation, and 60% of pupae reach pharate stages. Wing discs have a higher density (1.2 times) than seen i wild-type. The pharate adults show abnormalities in cuticular patterns and structures in all appendages. The adult wing of pharates are about 1.5 time the size of wild-type, with a higher cell number (2.9 times) and a higher trichome density (1.9 times). The shape of the wing is broad and short, enlarged in the anterior posterior axis compared with controls. The basal wing region is more affected than distal regions, with vein abnormalities and abnormal trichome polarity. The chaetae pattern of the wing margin and the position of the vein sensillae are fairly normal, although chaetae are blunter and thicker. Some apoptotic cells are seen in the wing blade. Somatic wing clones of homozygous ftk07918 in a M(2)24F1 background tend to fill one or several intervein sectors but a fraction do cross the dorsal ventral boundary. The clones tend to fill proximal regions of the wing. The distal borders of clones are rounded, and are smooth in contrast with the indented borders found in control clones. In all of these features ftk07918 and ftG-rv are more extreme than ft4. Clones can also cause outgrowths in the wing, generally in the proximal part of the wing blade. There are blisters that evaginate from the wing surface and have irregular trichome polarities, usually perpendicular to the clone border. Trichome density in clones is higher than wild-type clones - about 2.2 times that of wild-type, indicating that cell size is smaller than wild-type. Mutant clones that overlap veins differentiate thicker than normal vein ribbons. Chaetae within clones are smaller and blunter than wild-type. Somatic clones in the notum (of homozygous ftk07918 in a M(2)24F1 background) are large, with more cells than control clones, leading to an increase in the total notum surface. They contain many more chaetae (1.7 times wild-type) and higher cell density (1.4 times). Similar deviations occur in the legs and head capsule. tergite clones however show normal patterns of pigment chaetae and trichomes. ftk07918/ftk07918 somatic clones in the wing, in a rhove-1/vn1 background cover smaller territories than clones in wild-type. The clones appear in central and proximal regions of the wing blade, but distalise more than control ftk07918 clones. Clones may cover presumptive vein regions but never fail to differentiate vein histotype. ftk07918/ftk07918 somatic clones in the wing, in a Egfrt1 background are smaller than clones in a wild-type background. ftk07918/ftk07918 somatic clones in the wing, in a Egfr background show more cells than twin clones. ftk07918/ftk07918 somatic clones in the wing, in a N55e11/+ or a NAx-16/+ background do not depart from those in wild-type wings. wgNZ/ftk07918 somatic clones exhibit an additive phenotype of the two alleles. nub1/ftk07918 somatic clones are small and cause overgrowth, as in ftk07918 clones, but retain the extreme accommodation phenotype of nub1. Su(H)k07904/ftk07918 somatic clones show additive phenotypes. In ds38k,ftk07918 clones only epistatic effects of ftk07918 are seen. ftk07918/ft4 and ftk07918/ftG-rv larvae show a delay in pupariation of 1-2 days and have a maximal disc size similar to that of ftk07918 homozygotes. ftk07918,Su(H)k07904 doubly mutant discs show a phenotype intermediate between ftk07918 and Su(H)k07904 phenotypes.
Mutants show lethal overgrowth of the brain and imaginal discs.
Mutants exhibit visible disc abnormalities: discs are overgrown. Clones induced in wild type discs are abnormal: clones are larger than controls. Clones in the wing cause extra veins and vein thickening.
Lethality occurs during pupal and pharate adult stages. Brain is occasionally larger than wild type. Leg discs develop extra folds, hyperplastic outgrowth.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
NOT Enhanced by
Suppressed by
NOT suppressed by
Other
Phenotype Manifest In
Enhanced by
Statement
Reference
ftk07918 has wing disc phenotype, enhanceable by nub2
NOT Enhanced by
Statement
Reference
ftk07918 has wing | somatic clone phenotype, non-enhanceable by tkv1
ftk07918 has wing disc phenotype, non-enhanceable by EgfrE3
ftk07918 has wing disc phenotype, non-enhanceable by rhove-1
ftk07918 has wing disc phenotype, non-enhanceable by rlSem
ftk07918 has wing disc phenotype, non-enhanceable by vn1
NOT suppressed by
Statement
Reference
ftk07918 has wing | somatic clone phenotype, non-suppressible by tkv1
ftk07918 has wing disc phenotype, non-suppressible by EgfrE3
ftk07918 has wing disc phenotype, non-suppressible by rhove-1
ftk07918 has wing disc phenotype, non-suppressible by rlSem
ftk07918 has wing disc phenotype, non-suppressible by vn1
Additional Comments
Genetic Interactions
Statement
Reference
When ftk07918 somatic clones are made in the wing in a rhoScer\UAS.cdCa or phlScer\UAS.F179 background (driven by Scer\GAL4Bx-MS1096) clones are several times larger than ftk07918 clones in a wild-type background. The clones also show a homogeneous distribution in the wing. The polarity defects seen in ftk07918 clones in a wild-type background are not affected. This enhancement of the ftk07918 overgrowth phenotype is also seen when mutant eye and wing discs are made with clones. ftk07918 clones are induced in a dmP0 background at 72+-2h are severely reduced compared to those induced in wild-type. When ftk07918 clones are induced in a tkv1 background the size and allocation of clones is unaffected.
aprK568, ftk07918 double homozygotes are lethal (L1-2 phenoeffective phase). The double mutant combination nub2, ftk07918 causes a strong delay in pupation, allowing the wing discs to be considerably enlarged. These discs are larger than seen in ftk07918 alone. The addition of EgfrE3, EgfrE3, vn1 or rhove-1 has no effect on the ftk07918 wing disc phenotype.
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
Separable from: P{lacW}GlyPk07918. The overgrowth phenotype and lethality of ftk07918 are due to a second site mutation on the "l(2)k07918" chromosome and are not associated with the P{lacW}GlyPk07918 insertion.
Comments
Comments
This allele was listed in the BDGP database as a lethal or sterile line during the period 1994-1999, but was discarded from the gene disruption project prior to the summary publication (FBrf0111489). Reasons for excluding lines from the collection described in FBrf0111489 include presence of more than one P insertion on the mutant chromosome, separation of lethality (or sterility) from the location of the insertion, and loss of lethality (or sterility) from the stock. Further information is available from http://www.fruitfly.org/bfd/ and from Dr. Spradling (spradling@mail1.ciwemb.edu).
Lethality not caused by P{lacW}GlyPk07918.
P-element excision demonstrates the insertion to be the cause of lethality.
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (7)
References (13)