Flies homozygous for nmoP1 show ommatidia arrested at around 45[o] of rotation. The rate of rotation appears to be lower in these mutants compared to wild-type.
nmoP1 mutant flies exhibit wings that are smaller than wild-type and display a more rounded morphology and a held-out phenotype. Eyes are externally rough and narrower than wild-type. Tangential sections of those eyes reveal a high number of under-rotated ommatidia with a mean orientation angle of 59.3[o] with respect to the equator.
nmoP1 mutant eye discs display ommatidial rotation that is slower than in wild-type and that stop rotating prematurely. Apical shape change in the interommatidial cells, along with changes in cell area, are less evident in nmoP1 discs than in controls. The cone cell precursors also display abnormal behavior, with these cells being significantly more static in mutants than in controls.
Mutant ommatidia show reduced rotation of ommatidia compared to controls.
nmoP1/Df(3L)Exel6279 adults exhibit shorter circadian period of locomotor activity rhythms under constant darkness compared to controls.
Most neuromuscular junctions (NMJs) at muscles 6 and 7 lack branches containing more than five boutons in nmoP1/Df(3L)Exel6279 third instar larvae. There is a strong reduction in the number of type I synaptic boutons at muscles 6 and 7 and in the number of boutons at muscle 4 NMJs in the mutant larvae compared to wild type.
The number of boutons per muscle surface area is reduced at the neuromuscular junction in nmoP1/Df(3L)Exel6279, nmoP1/Df(3L)pbl-X1 and nmoDB24/nmoP1 third instar larvae.
Mutant ommatidial precursors rotate at a slower rate than wild-type ommatidial precursors in the developing eye. Initiation of ommatidial rotation and the first approximately 7[o] of rotation occurs normally in the mutant eye disc, but the average angle of rotation is significantly lower in the mutant eye discs than in wild-type from ommatidial row 5 onwards. The mutant ommatidia stall at 45[o] of rotation in the mutant eye disc, although in adults the average angle of rotation is 61[o] +/- 23 in mutant eyes.
No ectopic wing margin bristles are seen in nmoP1 mutant wings.
Ommatidia are arrested at approximately 45o to the equator.
In larval eye discs of nmoj147-1/nmoP1, ommatidial rotation is arrested at about 45o, instead of 60o-65o as in wild-type.
nmoP1 heterozygous flies (genetic background = Canton-S) have a small but significant decrease in geotaxis score compared to wild-type (Canton-S) flies.
nmoP1/nmoadk2 flies have a smaller eye than normal in which the ommatidia are square instead of the normal hexagonal shape and are generally flanked by 4 interommatidial bristles (instead of the usual 3 bristles at alternating corners). Slight extra vein material near the posterior crossvein is seen in a small percentage of nmoP1 flies. Flies do not show any noticeable polarity defects in the wing.
Eyes of nmo mutant flies are slightly narrowed anterior-posterior, and the facets are square rather than hexagonal. Pigment cell lattice is abnormal, especially at the corners, which have disarranged bristle and secondary pigment cells. Occasionally ommatidia are fused three to ten per eye. Most ommatidia have normal numbers of photoreceptor cells, although some lack a few. Viability is reduced to 10% of wild type and the angle of wings is 15o to 30o. In mutant eye discs the 5 cell precluster completes its first 45o rotation, but does not continue to the full 90o rotation. This also applies to the cone cells and primary pigment cells.