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General Information
Symbol
Dmel\orbmel
Species
D. melanogaster
Name
maternal effect lethal
FlyBase ID
FBal0035315
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Comment:

Approximate extent of a deletion of about 500bp, which starts at the P{PZ}orbdec insertion site.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Removal of the entire P-element and 500bp of orb genomic DNA from the P-element insertion site to within intron 2.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Homozygous females produce a low level (15%) of maternal effect embryonic lethality.

51% of embryos derived from homozygous females are undeveloped, 27% have a posterior patterning defects and 22% develop into adults. Those embryos that develop into adult females that are sterile. 65% of embryos derived from heterozygous female hatch.

100% of eggs laid by orbmel/orbF343 females are ventralised and unfertilised.

0% of eggs derived from orbmel/orbF343 females have wild-type dorsal appendages, 3.4% have fused dorsal appendages and 96.6% have no dorsal appendages. The eggs do not hatch.

Only 44% of eggs derived from homozygous females hatch. Only 6% of eggs derived from orbmel/orbF303 females hatch.

The pre-vitellogenic stages of oogenesis in mutant egg chambers appear to be completely normal. Eggs develop to become ventralised.

The restriction of the synaptonemal complex (SC) to one cell is delayed in orbmel mutants; both pro-oocytes can still be in synapsis in region 2b. This delay in oocyte determination can also give rise to misplaced oocytes.

Almost 20% of embryos derived from orbmel females hatch as larvae.

Females display reduction of dorsal eggshell structures, eggs develop posterior pattern defects.

Embryos display defects on the dorsal side of the embryo, fusion or absence of the dorsal appendages, reduction in the amount of orb mRNA enhances this phenotype. Some heterozygous orbmel/orbF343 embryos display absence of dorsal appendages, oocytes are small than normal and surrounded by columnar follicle cells and eggs are also smaller than normal. Homozygous embryos display abdominal segment deletions lack pole cells.

Dorsalization of chorion, posterior abdomen and pole cells fail to form.

orbmel/orbF343 causes lateralization. Eggs are shortened and rounded posteriorly like dorsalized eggs but like ventralized eggs their dorsal appendages are fused dorsally, in 49% of eggs, or absent, in 46% of the eggs. 37% of the eggs contain embryos.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference

orbmel has lethal | maternal effect | embryonic stage phenotype, enhanceable by wisp89/wisp[+]

orbmel has lethal | maternal effect | embryonic stage phenotype, enhanceable by wisp40/wisp[+]

orbmel has lethal | maternal effect | embryonic stage phenotype, enhanceable by wispKG05287/wisp[+]

orbmel has lethal | maternal effect | embryonic stage phenotype, enhanceable by hrgPAP45/hrg[+]

orbmel has lethal | maternal effect | embryonic stage phenotype, enhanceable by hrgPAP21/hrg[+]

Suppressed by
Phenotype Manifest In
Enhanced by
Statement
Reference

orbmel has ovary phenotype, enhanceable by hrgPAP21

orbmel has ovary phenotype, enhanceable by hrgPAP45

NOT Enhanced by
Statement
Reference

orbmel has phenotype, non-enhanceable by Mosunspecified

Suppressed by
Statement
Reference

orbF303/orbmel has oocyte phenotype, suppressible | partially by ypsJM2/ypsJM2

NOT suppressed by
Statement
Reference
NOT Enhancer of
Statement
Reference

orbmel/orbmel is a non-enhancer of egg | ventral phenotype of fs(1)K10unspecified

NOT Suppressor of
Statement
Reference

orbmel/orbmel is a non-suppressor of egg | ventral phenotype of fs(1)K10unspecified

Other
Additional Comments
Genetic Interactions
Statement
Reference

The percentage of maternal effect embryonic lethality seen in embryos derived from orbmel homozygous females is increased by heterozygosity for one of wisp89, wisp40, wispKG05287, hrgPAP45 or hrgPAP21. The percentage of ventralised embryos is not increased by heterozygosity for one copy of wisp89, wisp40 or wispKG05287, but is enhanced by heterozygosity for one copy of hrgPAP45 or hrgPAP21.

Oogenesis is blocked at stage 8 in 34% of hrgPAP21/+ ; orbmel females.

The maternal effect embryonic semi-lethality and ventralisation phenotypes due to orbmel/orbmel are partially suppressed when the mother also carries Pabp2EP2264/+ or Pabp255/+.

65% of embryos derived from BicC5/+ ; orbmel/+ females hatch. Of the non-hatching embryos, 39% are bicaudal and 52% have head defects.

Wild-type patterns of microtubule distribution and cytoplasmic streaming are restored in half of orbmel/orbF303 oocytes if they are also homozygous for ypsJM2.

hrgPAP21/+; orbmel and hrgPAP45/+; orbmel lay almost no eggs. In these females oogenesis stops most frequently at stage 7/8 after which egg chambers degenerate, even though one or two stage 14 oocytes per ovary can be observed.

The failure of many eggs derived from orbmel females to hatch is suppressed by ypsJM2/ypsJM2. The failure of most eggs derived from orbmel/orbF303 females to hatch is partially suppressed by ypsJM2/ypsJM2 (40% of eggs derived from the double mutant females hatch). The failure of eggs derived from orbmel/orbF343 females to hatch is very weakly suppressed by ypsJM2/ypsJM2; rare escapers complete embryogenesis and hatch. Eggs laid by ypsJM2 orbmel/ypsJM2 orbF303 females have fused, reduced or absent dorsal appendages. 5% of ypsJM2 orbmel/ypsJM2 orbF303 egg chambers are bipolar.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
Comments
Comments

Mutation does not affect posterior follicle cell determination.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Name Synonyms
maternal effect lethal
Secondary FlyBase IDs
    References (29)