Allele Dmel\phl::tor^13D.hs.sev

General Information
Symbol	Dmel\phl::tor^13D.hs.sev	Species	D. melanogaster
Name		FlyBase ID	FBal0035393
Feature type	allele	Associated gene	Dmel\phl::tor

Allele class
Mutagen	in vitro construct - coding region fusion, in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - coding region fusion (Dickson et al., 1992, Reichman-Fried et al., 1994, Vincent et al., 1998) in vitro construct - regulatory fusion (Dickson et al., 1992, Reichman-Fried et al., 1994, Chang and Rubin, 1997, Vincent et al., 1998)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype	tor^13D (Dickson et al., 1992)
Nature of the lesion	Statement Reference A constitutively active form of phl is expressed under the control of a sev enhancer. (Chang and Rubin, 1997) phl intracellular kinase domain replaces the equivalent region from tor^13D, expression of the chimera is driven by an Hsp70 promoter and a single copy of the 1.2kb sev enhancer. (Reichman-Fried et al., 1994) Construct: phl kinase domain fused to the signal sequence, intracellular and transmembrane domains carrying the amino acid replacement characteristic of the tor^13D protein, expressed from the sev enhancer and a heat shock promoter. (Dickson et al., 1992)
Carried in construct	P{sE-raf^tor4021} (Roy et al., 2002, Reichman-Fried et al., 1994, Chang et al., 1995, Bai et al., 2001, Therrien et al., 1999, Casci et al., 1999, Lu and Li, 1999, Dickson et al., 1992, Vincent et al., 1998, Kauffmann et al., 1995, Lai et al., 1996, Karim et al., 1996, Allard et al., 1996, Wassarman et al., 1996, Chang et al., 1994, Therrien et al., 1995, Chang and Rubin, 1997, Peverali et al., 1996)
Cytology
Phenotypic Data
Phenotypic Class
	visible (Lai et al., 1996, Bai et al., 2001, Kauffmann et al., 1995, Lu and Li, 1999, Roy et al., 2002) visible \| dominant (Chang and Rubin, 1997)
Phenotype Manifest In
	eye (Wassarman et al., 1996, Lai et al., 1996, Bai et al., 2001, Chang and Rubin, 1997, Therrien et al., 1998, Lu and Li, 1999, Roy et al., 2002) photoreceptor cell R7 (Chang et al., 1995, Kauffmann et al., 1995) photoreceptor cell R7 \| supernumerary (Roy et al., 2002)
Detailed Description
	Statement Reference Flies expressing phl::tor^13D.hs.sev have extra photoreceptor R7 cells, which result in a rough eye phenotype. (Roy et al., 2002) The phl::tor^13D.hs.sev phenotype is unaffected by halving the dose of sty⁺. (Casci et al., 1999) Rough eye phenotype. (Therrien et al., 1998, Bai et al., 2001) Flies carrying one copy of phl::tor^13D.hs.sev have rough eyes. (Chang and Rubin, 1997) ttk mutations have no significant effect on the rough eye phenotype. (Lai et al., 1996) Rough eye phenotype, with supernumerary R7 cells. (Chang et al., 1995) Supernumerary R7 cell formation. Injection of a Ras CAAX peptidomimetic, FTI-254, fails to rescue the mutant eye phenotype. (Kauffmann et al., 1995) Partial rescue of tracheal migration defect in btl homozygotes. (Reichman-Fried et al., 1994) Dominant rough eye phenotype. (Dickson et al., 1992)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference phl::tor^13D.hs.sev has visible phenotype, enhanceable by Df(2R)nap8 (Lu and Li, 1999) phl::tor^13D.hs.sev has visible phenotype, enhanceable by Df(2R)nap9 (Lu and Li, 1999) phl::tor^13D.hs.sev has visible phenotype, enhanceable by ed[+]/ed^1X5 (Bai et al., 2001) phl::tor^13D.hs.sev has visible phenotype, enhanceable by In(2R)bw^VDe2LCy^R (Lu and Li, 1999) phl::tor^13D.hs.sev has visible phenotype, enhanceable by ksr::tor^{ΔNksr.hs.sev} (Roy et al., 2002) phl::tor^13D.hs.sev has visible phenotype, enhanceable by Src42A^7-4 (Lu and Li, 1999) phl::tor^13D.hs.sev has visible phenotype, enhanceable by Src42A^18-2 (Lu and Li, 1999)
NOT Enhanced by
Statement Reference phl::tor^13D.hs.sev has visible phenotype, non-enhanceable by Src42A^1.15 (Lu and Li, 1999) phl::tor^13D.hs.sev has visible phenotype, non-enhanceable by Src42A^9-4 (Lu and Li, 1999)
Other
Statement Reference Src42A^Su(phl)1-1, phl::tor^13D.hs.sev has lethal \| dominant \| pupal stage phenotype (Lu and Li, 1999, Lu and Li, 1999)
Phenotype Manifest In
Enhanced by
Statement Reference phl::tor^13D.hs.sev has eye phenotype, enhanceable by Df(2R)nap8 (Lu and Li, 1999) phl::tor^13D.hs.sev has eye phenotype, enhanceable by Df(2R)nap9 (Lu and Li, 1999) phl::tor^13D.hs.sev has eye phenotype, enhanceable by ed[+]/ed^1X5 (Bai et al., 2001) phl::tor^13D.hs.sev has eye phenotype, enhanceable by In(2R)bw^VDe2LCy^R (Lu and Li, 1999) phl::tor^13D.hs.sev has eye phenotype, enhanceable by ksr::tor^{ΔNksr.hs.sev} (Roy et al., 2002) phl::tor^13D.hs.sev has eye phenotype, enhanceable by Src42A^7-4 (Lu and Li, 1999) phl::tor^13D.hs.sev has eye phenotype, enhanceable by Src42A^18-2 (Lu and Li, 1999) phl::tor^13D.hs.sev has phenotype, enhanceable by aop^E-867 (Karim et al., 1996) phl::tor^13D.hs.sev has phenotype, enhanceable by aop^yan-XE18 (Karim et al., 1996)
NOT Enhanced by
Statement Reference phl::tor^13D.hs.sev has eye phenotype, non-enhanceable by dos^1.46 (Herbst et al., 1996) phl::tor^13D.hs.sev has eye phenotype, non-enhanceable by dos^2.42 (Herbst et al., 1996) phl::tor^13D.hs.sev has eye phenotype, non-enhanceable by dos^unspecified (Herbst et al., 1996) phl::tor^13D.hs.sev has eye phenotype, non-enhanceable by Src42A^1.15 (Lu and Li, 1999) phl::tor^13D.hs.sev has eye phenotype, non-enhanceable by Src42A^9-4 (Lu and Li, 1999) phl::tor^13D.hs.sev has phenotype, non-enhanceable by cnk^{Nterm.hs.sev.T:Zzzz\FLAG} (Therrien et al., 1999) phl::tor^13D.hs.sev has phenotype, non-enhanceable by cnk^XE-1040 (Therrien et al., 1998)
Suppressed by
Statement Reference phl::tor^13D.hs.sev has eye phenotype, suppressible by 14-3-3ε^S-696 (Chang and Rubin, 1997) phl::tor^13D.hs.sev has eye phenotype, suppressible by cnk^{Cterm.hs.sev.T:Zzzz\FLAG} (Therrien et al., 1999) phl::tor^13D.hs.sev has eye phenotype, suppressible by cnk^{hs.sev.T:Zzzz\FLAG} (Therrien et al., 1999, Therrien et al., 1998) phl::tor^13D.hs.sev has eye phenotype, suppressible by mts^XE-2258 (Wassarman et al., 1996) phl::tor^13D.hs.sev has phenotype, suppressible by csw^{G547E.hs.2sev} (Allard et al., 1996) phl::tor^13D.hs.sev has phenotype, suppressible by phyl¹⁵ (Chang et al., 1995) phl::tor^13D.hs.sev has phenotype, suppressible by phyl²¹⁵² (Chang et al., 1995) phl::tor^13D.hs.sev has phenotype, suppressible by phyl²³⁶⁶ (Chang et al., 1995) phl::tor^13D.hs.sev has phenotype, suppressible by phyl²⁸²⁹ (Chang et al., 1995)
NOT suppressed by
Statement Reference phl::tor^13D.hs.sev has eye phenotype, non-suppressible by dos^1.46 (Herbst et al., 1996) phl::tor^13D.hs.sev has eye phenotype, non-suppressible by dos^2.42 (Herbst et al., 1996) phl::tor^13D.hs.sev has eye phenotype, non-suppressible by dos^unspecified (Herbst et al., 1996) phl::tor^13D.hs.sev has eye phenotype, non-suppressible by ksr^unspecified (Therrien et al., 1995) phl::tor^13D.hs.sev has eye phenotype, non-suppressible by βggt-I^unspecified (Therrien et al., 1995) phl::tor^13D.hs.sev has phenotype, non-suppressible by cnk^{Nterm.hs.sev.T:Zzzz\FLAG} (Therrien et al., 1999) phl::tor^13D.hs.sev has phenotype, non-suppressible by cnk^XE-1040 (Therrien et al., 1998)
Suppressor of
Statement Reference phl::tor^13D.hs.sev is a suppressor \| partially of embryonic/larval tracheal system phenotype of btl^LG19 (Vincent et al., 1998) phl::tor^13D.hs.sev is a suppressor \| partially of embryonic/larval tracheal system phenotype of stumps^09904b (Vincent et al., 1998) phl::tor^13D.hs.sev is a suppressor \| partially of terminalia \| germline clone phenotype of ksr^S-638 (Therrien et al., 1995) phl::tor^13D.hs.sev is a suppressor of embryonic abdominal segment 8 phenotype of phl¹¹ (Li et al., 1998) phl::tor^13D.hs.sev is a suppressor of filzkorper phenotype of phl¹¹ (Li et al., 1998)
NOT Suppressor of
Statement Reference phl::tor^13D.hs.sev is a non-suppressor of cephalopharyngeal skeleton phenotype of phl¹¹ (Li et al., 1998) phl::tor^13D.hs.sev is a non-suppressor of eye phenotype of ksr^unspecified (Therrien et al., 1995) phl::tor^13D.hs.sev is a non-suppressor of eye phenotype of βggt-I^unspecified (Therrien et al., 1995)
Other
Statement Reference phl::tor^13D.hs.sev, tor^rv66 has filzkorper phenotype (Li et al., 1998)
Additional Comments
Genetic Interactions
Statement Reference The rough eye phenotype caused by phl::tor^13D.hs.sev is robustly enhanced by ksr::tor^{ΔNksr.hs.sev}. (Roy et al., 2002) The rough eye phenotype of phl::tor^13D.hs.sev is enhanced by Src42A^18-2, Src42A^7-4, Df(2R)nap8, Df(2R)nap9 or In(2R)bw^VDe2LCy^R. The rough eye phenotype of phl::tor^13D.hs.sev is not enhanced by Src42A^1.15 or Src42A^9-4. Lethality at the pupal stage is seen in Src42A^Su(phl)1-1 phl::tor^13D.hs.sev double heterozygotes. (Lu and Li, 1999) When injected into the posterior of phl¹¹ null embryos, phl::tor^13D.hs.sev is highly efficient at rescuing posterior cuticular structures including A8 and the filzkorper. phl::tor^13D.hs.sev is much less effective in rescuing Ras85D^ΔC40B null mutants though some rescue is seen at high concentrations of phl::tor^13D.hs.sev. When injected into tor^rv66 at several concentrations generally around 5% of embryos were rescued. (Li et al., 1998) Expression of phl::tor^13D.hs.sev in btl^LG19 or stumps^09904b mutant embryos partially rescues tracheal morphogenesis. (Vincent et al., 1998) The phl::tor^13D.hs.sev eye phenotype is dominantly suppressed by 14-3-3ε^S-696. (Chang and Rubin, 1997) Extra R7-cell phenotype is suppressed by csw^{G547E.hs.2sev}. (Allard et al., 1996) Eye phenotype is weakly suppressed by mts^XE-2258. (Wassarman et al., 1996) Eye phenotype is unaffected by mutations in βggt-I or ksr. Embryos derived from germline clones of ksr^S-638 reveal severe terminal defects which can be partially rescued by the injection of mRNA generated from phl::tor^13D.hs.sev. (Therrien et al., 1995)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer	Dickson.

Comments
	phl product is artificially targetted to the membrane through tor N-terminal sequences. (Li et al., 1998) Carried in a plasmid, expressed in Schneider S2 cells with Hsp83 mutants to study Hsp83 binding to phl using Western blots. (van der Straten et al., 1997)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 6 )
Reported As
Symbol Synonym	DrafΔN^tor4021 (Li et al., 1998) phl::tor^13D.hs.sev RAF^TOR (Herbst et al., 1996) TOR⁴⁰²¹-RAF (Raabe, 2000) Tor⁴⁰²¹RAFc (Douziech et al., 2003) tor-Draf (Reichman-Fried et al., 1994)
Name Synonym
Secondary FlyBase IDs

References ( 24 )

Generate a list of	All references relating to this allele ( 24 )

List References by type	Research paper ( 21 ) Review ( 2 ) Letter ( 1 )
Recent research papers (0)
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Allele Dmel\phl::tor13D.hs.sev

Allele Dmel\phl::tor^13D.hs.sev