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General Information
Symbol
Dmel\Raf::tor13D.hs.sev
Species
D. melanogaster
Name
FlyBase ID
FBal0035393
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
sev-tor4021Raf
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

Construct: phl kinase domain fused to the signal sequence, intracellular and transmembrane domains carrying the amino acid replacement characteristic of the tor13D protein, expressed from the sev enhancer and a heat shock promoter.

Allele components
Product class / Tool use(s)
Regulatory region(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Flies expressing phl::tor13D.hs.sev have extra photoreceptor R7 cells, which result in a rough eye phenotype.

The phl::tor13D.hs.sev phenotype is unaffected by halving the dose of sty+.

Flies carrying one copy of phl::tor13D.hs.sev have rough eyes.

ttk mutations have no significant effect on the rough eye phenotype.

Rough eye phenotype, with supernumerary R7 cells.

Supernumerary R7 cell formation. Injection of a Ras CAAX peptidomimetic, FTI-254, fails to rescue the mutant eye phenotype.

Partial rescue of tracheal migration defect in btl homozygotes.

Dominant rough eye phenotype.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference

Raf::tor13D.hs.sev has visible phenotype, enhanceable by ed[+]/ed1X5

Raf::tor13D.hs.sev has visible phenotype, enhanceable by Df(2R)nap8

Raf::tor13D.hs.sev has visible phenotype, enhanceable by Df(2R)nap9

Raf::tor13D.hs.sev has visible phenotype, enhanceable by Src42A18-2

Raf::tor13D.hs.sev has visible phenotype, enhanceable by Src42A7-4

NOT Enhanced by
Statement
Reference

Raf::tor13D.hs.sev has visible phenotype, non-enhanceable by Src42A1.15

Raf::tor13D.hs.sev has visible phenotype, non-enhanceable by Src42A9-4

Other
Phenotype Manifest In
Enhanced by
Statement
Reference

Raf::tor13D.hs.sev has eye phenotype, enhanceable by ed[+]/ed1X5

Raf::tor13D.hs.sev has eye phenotype, enhanceable by Df(2R)nap8

Raf::tor13D.hs.sev has eye phenotype, enhanceable by Df(2R)nap9

Raf::tor13D.hs.sev has eye phenotype, enhanceable by Src42A18-2

Raf::tor13D.hs.sev has eye phenotype, enhanceable by Src42A7-4

Raf::tor13D.hs.sev has phenotype, enhanceable by aopE-867

Raf::tor13D.hs.sev has phenotype, enhanceable by aopyan-XE18

NOT Enhanced by
Statement
Reference

Raf::tor13D.hs.sev has eye phenotype, non-enhanceable by Src42A1.15

Raf::tor13D.hs.sev has eye phenotype, non-enhanceable by Src42A9-4

Raf::tor13D.hs.sev has phenotype, non-enhanceable by cnkXE-1040

Raf::tor13D.hs.sev has eye phenotype, non-enhanceable by dos2.42

Raf::tor13D.hs.sev has eye phenotype, non-enhanceable by dos1.46

Raf::tor13D.hs.sev has eye phenotype, non-enhanceable by dosunspecified

Suppressed by
Statement
Reference
NOT suppressed by
Statement
Reference

Raf::tor13D.hs.sev has phenotype, non-suppressible by cnkXE-1040

Raf::tor13D.hs.sev has eye phenotype, non-suppressible by dos2.42

Raf::tor13D.hs.sev has eye phenotype, non-suppressible by dos1.46

Raf::tor13D.hs.sev has eye phenotype, non-suppressible by dosunspecified

Raf::tor13D.hs.sev has eye phenotype, non-suppressible by ksrunspecified

Suppressor of
NOT Suppressor of
Statement
Reference

Raf::tor13D.hs.sev is a non-suppressor of eye phenotype of ksrunspecified

Raf::tor13D.hs.sev is a non-suppressor of eye phenotype of βggt-Iunspecified

Other
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

The rough eye phenotype caused by phl::tor13D.hs.sev is robustly enhanced by ksr::torΔNksr.hs.sev.

The rough eye phenotype of phl::tor13D.hs.sev is enhanced by Src42A18-2, Src42A7-4, Df(2R)nap8, Df(2R)nap9 or In(2R)bwVDe2LCyR. The rough eye phenotype of phl::tor13D.hs.sev is not enhanced by Src42A1.15 or Src42A9-4. Lethality at the pupal stage is seen in Src42ASu(phl)1-1 phl::tor13D.hs.sev double heterozygotes.

When injected into the posterior of phl11 null embryos, phl::tor13D.hs.sev is highly efficient at rescuing posterior cuticular structures including A8 and the filzkorper. phl::tor13D.hs.sev is much less effective in rescuing Ras85DΔC40B null mutants though some rescue is seen at high concentrations of phl::tor13D.hs.sev. When injected into torrv66 at several concentrations generally around 5% of embryos were rescued.

Expression of phl::tor13D.hs.sev in btlLG19 or stumps09904b mutant embryos partially rescues tracheal morphogenesis.

The phl::tor13D.hs.sev eye phenotype is dominantly suppressed by 14-3-3εS-696.

Extra R7-cell phenotype is suppressed by cswG547E.hs.2sev.

Eye phenotype is weakly suppressed by mtsXE-2258.

Eye phenotype is unaffected by mutations in βggt-I or ksr. Embryos derived from germline clones of ksrS-638 reveal severe terminal defects which can be partially rescued by the injection of mRNA generated from phl::tor13D.hs.sev.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
Comments
Comments

phl product is artificially targetted to the membrane through tor N-terminal sequences.

Carried in a plasmid, expressed in Schneider S2 cells with Hsp83 mutants to study Hsp83 binding to phl using Western blots.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (7)
Reported As
Symbol Synonym
DrafΔNtor4021
Raf::tor13D.hs.sev
TOR4021-RAF
phl::tor13D.hs.sev
Name Synonyms
Secondary FlyBase IDs
    References (24)