FlyBase Allele Report: Dmel\Raf::tor[13D.hs.sev]

General Information

Symbol

Dmel\Raf::tor^13D.hs.sev

Species

D. melanogaster

Name

FlyBase ID

FBal0035393

Feature type

allele

Associated gene

Raf::tor

Associated Insertion(s)

Carried in Construct

P{sE-raf^tor4021}

Also Known As

sev-tor⁴⁰²¹Raf

Key Links

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Vincent et al., 1998, Chang and Rubin, 1997, Reichman-Fried et al., 1994, Dickson et al., 1992)

Progenitor genotype

tor^13D

(Dickson et al., 1992)

Carried in construct

P{sE-raf^tor4021}

Cytology

Description

Construct: phl kinase domain fused to the signal sequence, intracellular and transmembrane domains carrying the amino acid replacement characteristic of the tor^13D protein, expressed from the sev enhancer and a heat shock promoter.

(Dickson et al., 1992)

Allele components

Component

Use(s)

Regulatory region(s)

Hsp70

sev

Encoded product / tool

Raf::tor

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible

(Roy et al., 2002, Bai et al., 2001, Lu and Li, 1999, Lai et al., 1996, Kauffmann et al., 1995)

visible | dominant

(Chang and Rubin, 1997)

Phenotype Manifest In

eye

(Roy et al., 2002, Bai et al., 2001, Lu and Li, 1999, Therrien et al., 1998, Chang and Rubin, 1997, Lai et al., 1996, Wassarman et al., 1996)

photoreceptor cell R7

(Chang et al., 1995, Kauffmann et al., 1995)

photoreceptor cell R7 | increased number

(Roy et al., 2002)

Detailed Description

Statement

Reference

Flies expressing phl::tor^13D.hs.sev have extra photoreceptor R7 cells, which result in a rough eye phenotype.

(Roy et al., 2002)

Rough eye phenotype.

(Bai et al., 2001, Therrien et al., 1998)

The phl::tor^13D.hs.sev phenotype is unaffected by halving the dose of sty⁺.

(Casci et al., 1999)

Flies carrying one copy of phl::tor^13D.hs.sev have rough eyes.

(Chang and Rubin, 1997)

ttk mutations have no significant effect on the rough eye phenotype.

(Lai et al., 1996)

Rough eye phenotype, with supernumerary R7 cells.

(Chang et al., 1995)

Supernumerary R7 cell formation. Injection of a Ras CAAX peptidomimetic, FTI-254, fails to rescue the mutant eye phenotype.

(Kauffmann et al., 1995)

Partial rescue of tracheal migration defect in btl homozygotes.

(Reichman-Fried et al., 1994)

Dominant rough eye phenotype.

(Dickson et al., 1992)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Raf::tor^13D.hs.sev has visible phenotype, enhanceable by ksr::tor^{ΔNksr.hs.sev}

(Roy et al., 2002)

Raf::tor^13D.hs.sev has visible phenotype, enhanceable by ed[+]/ed^1X5

(Bai et al., 2001)

Raf::tor^13D.hs.sev has visible phenotype, enhanceable by Src42A^18-2

(Lu and Li, 1999)

Raf::tor^13D.hs.sev has visible phenotype, enhanceable by Src42A^7-4

(Lu and Li, 1999)

Raf::tor^13D.hs.sev has visible phenotype, enhanceable by Df(2R)nap8

(Lu and Li, 1999)

Raf::tor^13D.hs.sev has visible phenotype, enhanceable by Df(2R)nap9

(Lu and Li, 1999)

Raf::tor^13D.hs.sev has visible phenotype, enhanceable by In(2R)bw^VDe2LCy^R

(Lu and Li, 1999)

NOT Enhanced by

Statement

Reference

Raf::tor^13D.hs.sev has visible phenotype, non-enhanceable by Src42A^1.15

(Lu and Li, 1999)

Raf::tor^13D.hs.sev has visible phenotype, non-enhanceable by Src42A^9-4

(Lu and Li, 1999)

Other

Statement

Reference

Raf::tor^13D.hs.sev, Src42A^Su(Raf)1-1 has lethal | dominant | pupal stage phenotype

(Lu and Li, 1999)

Phenotype Manifest In

Enhanced by

Statement

Reference

Raf::tor^13D.hs.sev has eye phenotype, enhanceable by ksr::tor^{ΔNksr.hs.sev}

(Roy et al., 2002)

Raf::tor^13D.hs.sev has eye phenotype, enhanceable by ed[+]/ed^1X5

(Bai et al., 2001)

Raf::tor^13D.hs.sev has eye phenotype, enhanceable by Src42A^18-2

(Lu and Li, 1999)

Raf::tor^13D.hs.sev has eye phenotype, enhanceable by Src42A^7-4

(Lu and Li, 1999)

Raf::tor^13D.hs.sev has eye phenotype, enhanceable by Df(2R)nap8

(Lu and Li, 1999)

Raf::tor^13D.hs.sev has eye phenotype, enhanceable by Df(2R)nap9

(Lu and Li, 1999)

Raf::tor^13D.hs.sev has eye phenotype, enhanceable by In(2R)bw^VDe2LCy^R

(Lu and Li, 1999)

Raf::tor^13D.hs.sev has phenotype, enhanceable by aop^E-867

(Karim et al., 1996)

Raf::tor^13D.hs.sev has phenotype, enhanceable by aop^yan-XE18

(Karim et al., 1996)

NOT Enhanced by

Statement

Reference

Raf::tor^13D.hs.sev has eye phenotype, non-enhanceable by Src42A^1.15

(Lu and Li, 1999)

Raf::tor^13D.hs.sev has eye phenotype, non-enhanceable by Src42A^9-4

(Lu and Li, 1999)

Raf::tor^13D.hs.sev has phenotype, non-enhanceable by cnk^{Nterm.hs.sev.Tag:FLAG}

(Therrien et al., 1999)

Raf::tor^13D.hs.sev has phenotype, non-enhanceable by cnk^XE-1040

(Therrien et al., 1998)

Raf::tor^13D.hs.sev has eye phenotype, non-enhanceable by dos^2.42

(Herbst et al., 1996)

Raf::tor^13D.hs.sev has eye phenotype, non-enhanceable by dos^1.46

(Herbst et al., 1996)

Raf::tor^13D.hs.sev has eye phenotype, non-enhanceable by dos^unspecified

(Herbst et al., 1996)

Suppressed by

Statement

Reference

Raf::tor^13D.hs.sev has eye phenotype, suppressible by cnk^{hs.sev.Tag:FLAG}

(Therrien et al., 1999, Therrien et al., 1998)

Raf::tor^13D.hs.sev has eye phenotype, suppressible by cnk^{Cterm.hs.sev.Tag:FLAG}

(Therrien et al., 1999)

Raf::tor^13D.hs.sev has eye phenotype, suppressible by 14-3-3ε^S-696

(Chang and Rubin, 1997)

Raf::tor^13D.hs.sev has phenotype, suppressible by csw^{G547E.hs.2sev}

(Allard et al., 1996)

Raf::tor^13D.hs.sev has eye phenotype, suppressible by mts^XE-2258

(Wassarman et al., 1996)

Raf::tor^13D.hs.sev has phenotype, suppressible by phyl¹⁵

(Chang et al., 1995)

Raf::tor^13D.hs.sev has phenotype, suppressible by phyl²¹⁵²

(Chang et al., 1995)

Raf::tor^13D.hs.sev has phenotype, suppressible by phyl²³⁶⁶

(Chang et al., 1995)

Raf::tor^13D.hs.sev has phenotype, suppressible by phyl²⁸²⁹

(Chang et al., 1995)

NOT suppressed by

Statement

Reference

Raf::tor^13D.hs.sev has phenotype, non-suppressible by cnk^{Nterm.hs.sev.Tag:FLAG}

(Therrien et al., 1999)

Raf::tor^13D.hs.sev has phenotype, non-suppressible by cnk^XE-1040

(Therrien et al., 1998)

Raf::tor^13D.hs.sev has eye phenotype, non-suppressible by dos^2.42

(Herbst et al., 1996)

Raf::tor^13D.hs.sev has eye phenotype, non-suppressible by dos^1.46

(Herbst et al., 1996)

Raf::tor^13D.hs.sev has eye phenotype, non-suppressible by dos^unspecified

(Herbst et al., 1996)

Raf::tor^13D.hs.sev has eye phenotype, non-suppressible by βggt-I^unspecified

(Therrien et al., 1995)

Raf::tor^13D.hs.sev has eye phenotype, non-suppressible by ksr^unspecified

(Therrien et al., 1995)

Suppressor of

Statement

Reference

Raf::tor^13D.hs.sev is a suppressor of filzkorper phenotype of Raf¹¹

(Li et al., 1998)

Raf::tor^13D.hs.sev is a suppressor of embryonic abdominal segment 8 phenotype of Raf¹¹

(Li et al., 1998)

Raf::tor^13D.hs.sev is a suppressor of larval tracheal system phenotype of btl^LG19

(Vincent et al., 1998)

Raf::tor^13D.hs.sev is a suppressor of larval tracheal system phenotype of stumps^09904b

(Vincent et al., 1998)

Raf::tor^13D.hs.sev is a suppressor of terminalia | germline clone phenotype of ksr^S-638

(Therrien et al., 1995)

NOT Suppressor of

Statement

Reference

Raf::tor^13D.hs.sev is a non-suppressor of cephalopharyngeal skeleton phenotype of Raf¹¹

(Li et al., 1998)

Raf::tor^13D.hs.sev is a non-suppressor of eye phenotype of ksr^unspecified

(Therrien et al., 1995)

Raf::tor^13D.hs.sev is a non-suppressor of eye phenotype of βggt-I^unspecified

(Therrien et al., 1995)

Other

Statement

Reference

Raf::tor^13D.hs.sev, tor^rv66 has filzkorper phenotype

(Li et al., 1998)

Additional Comments

Genetic Interactions

Statement

Reference

The rough eye phenotype caused by phl::tor^13D.hs.sev is robustly enhanced by ksr::tor^{ΔNksr.hs.sev}.

(Roy et al., 2002)

The rough eye phenotype of phl::tor^13D.hs.sev is enhanced by Src42A^18-2, Src42A^7-4, Df(2R)nap8, Df(2R)nap9 or In(2R)bw^VDe2LCy^R. The rough eye phenotype of phl::tor^13D.hs.sev is not enhanced by Src42A^1.15 or Src42A^9-4. Lethality at the pupal stage is seen in Src42A^Su(phl)1-1 phl::tor^13D.hs.sev double heterozygotes.

(Lu and Li, 1999)

When injected into the posterior of phl¹¹ null embryos, phl::tor^13D.hs.sev is highly efficient at rescuing posterior cuticular structures including A8 and the filzkorper. phl::tor^13D.hs.sev is much less effective in rescuing Ras85D^ΔC40B null mutants though some rescue is seen at high concentrations of phl::tor^13D.hs.sev. When injected into tor^rv66 at several concentrations generally around 5% of embryos were rescued.

(Li et al., 1998)

Expression of phl::tor^13D.hs.sev in btl^LG19 or stumps^09904b mutant embryos partially rescues tracheal morphogenesis.

(Vincent et al., 1998)

The phl::tor^13D.hs.sev eye phenotype is dominantly suppressed by 14-3-3ε^S-696.

(Chang and Rubin, 1997)

Extra R7-cell phenotype is suppressed by csw^{G547E.hs.2sev}.

(Allard et al., 1996)

Eye phenotype is weakly suppressed by mts^XE-2258.

(Wassarman et al., 1996)

Eye phenotype is unaffected by mutations in βggt-I or ksr. Embryos derived from germline clones of ksr^S-638 reveal severe terminal defects which can be partially rescued by the injection of mRNA generated from phl::tor^13D.hs.sev.

(Therrien et al., 1995)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Dickson.

(Reichman-Fried et al., 1994)

Comments

phl product is artificially targetted to the membrane through tor N-terminal sequences.

(Li et al., 1998)

Carried in a plasmid, expressed in Schneider S2 cells with Hsp83 mutants to study Hsp83 binding to phl using Western blots.

(van der Straten et al., 1997)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

DrafΔN^tor4021

(Li et al., 1998)

RAF^TOR

(Herbst et al., 1996)

Raf::tor^13D.hs.sev

TOR⁴⁰²¹-RAF

(Raabe, 2000)

Tor⁴⁰²¹RAFc

(Douziech et al., 2003)

phl::tor^13D.hs.sev

tor-Draf

(Reichman-Fried et al., 1994)

Name Synonyms

Secondary FlyBase IDs

References (24)

Report Sections

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