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General Information
Symbol
Dmel\pnt1277
Species
D. melanogaster
Name
FlyBase ID
FBal0035442
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
pnt-lacZ, pntP2-lacZ
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

12kb P-element transposon insertion into the middle of exon 1 at position +365 of the P2 pnt transcript.

Insertion components
P{lacW}pnt1277
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

pntΔ88/pnt1277 flies have weakly rough eyes.

pntΔ88/pnt1277 flies have rough eyes and show a loss of photoreceptor cells.

Many pntΔ88/pnt1277 ommatidia over- or under-rotate; the variance (s.d.) in degree of rotation is greater than in wild type in adult eyes.

pnt1277/pnt1230 eye discs show polarity defects (10% of ommatidial clusters are inverted and 9% are symmetrical).

pnt1277/pntΔ88 eye discs show polarity defects (6.7% of ommatidial clusters are inverted and 6% are symmetrical).

pnt1277/pntΔ88 flies display a weak rough eye phenotype but pnt1277/pnt07825 flies have normal eyes.

In pnt1277/pntΔ88 mutant adults defects are seen in the eye. Under-recruitment of phtoreceptors is common, though in those ommatidia that are correctly specified, mis-rotations are seen.

pnt1277/pntΔ88 show reduced number of R7 photoreceptors.

90% of the ommatidia in pntΔ88/pnt1277 flies lack the R7 photoreceptor and/or some outer photoreceptor cells.

Viable. Heterozygotes with pntΔ88 lack one to three photoreceptor cells in most ommatidia. The R7 precursor can be rescued by pnt P2 transcript expressed by sev enhancers but the mutant phenotype is enhanced by pntT151A.Scer\UAS expressed by sev enhancers.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference
Suppressed by
Statement
Reference

pnt1277/pntΔ88 has visible phenotype, suppressible by Bap170hfl1/Bap170[+]

Suppressor of
Statement
Reference

pnt1277 is a suppressor of visible phenotype of fafBX4

Phenotype Manifest In
Enhanced by
Statement
Reference
NOT Enhanced by
Statement
Reference

pnt1277/pntΔ88 has eye phenotype, non-enhanceable by Ptp61F[+]/Ptp61F4-10

Suppressed by
NOT suppressed by
Statement
Reference

pnt1277/pntΔ88 has eye phenotype, non-suppressible by Ptp61F[+]/Ptp61F4-10

Enhancer of
Suppressor of
Statement
Reference

pnt1277 is a suppressor of phenotype of JraAsp.hs.sev

Additional Comments
Genetic Interactions
Statement
Reference

Bap170hfl1/+ suppresses the loss of photoreceptor cells seen in pntΔ88/pnt1277 flies.

Expression of Bap170C1.Scer\UAS under the control of Scer\GAL4elav.PU enhances the rough eye phenotype and loss of photoreceptor cells seen in pntΔ88/pnt1277 flies.

ed1X5 dominantly enhances the phenotype of mis-rotation of ommatidia that is seen in pntΔ88/pnt1277 animals.

fredH24 dominantly suppresses the phenotype of mis-rotation of ommatidia that is seen in pntΔ88/pnt1277 animals.

The rough eye phenotype of flies expressing GugScer\UAS.cCa under the control of Scer\GAL4GMR.PF is enhanced in both a pnt1277/pntΔ88 and a pnt1277/pnt07825 background.

The extra outer photoreceptor cell phenotype seen in fafBX4 homozygotes is suppressed by pnt1277, with the average number of outer photoreceptor cells per ommatidium being reduced to 6.2.

pntΔ88/pnt1277 or pnt1230/pnt1277 completely suppresses the ectopic R7 rough eye phenotype of JraAsp.hs.sev.

Xenogenetic Interactions
Statement
Reference

The eye phenotype of pntΔ88/pnt1277 flies is partially rescued when Ggal\ets2Scer\UAS.cAa or ALV\v-etsScer\UAS.cAa is expressed using Scer\GAL4hs.2sev.

Complementation and Rescue Data
Partially rescued by
Comments

The eye phenotype of pntΔ88/pnt1277 flies is partially rescued when pntP1.Scer\UAS is expressed using Scer\GAL4hs.2sev at 17oC.

The R7 precursor can be rescued by pnt P2 transcript expressed by sev enhancers but the mutant phenotype is enhanced by pntT151A.Scer\UAS expressed by sev enhancers.

Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
Comments
Comments

Allelic series of pnt allele that affect the P2 transcript: pnt1277 < pnt1202 < pnt1208 < pnt1230 <= pntT9 < pntT5.

Weak P2-specific pnt mutant.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (14)