Allele Dmel\pnt¹²⁷⁷

General Information
Symbol	Dmel\pnt¹²⁷⁷	Species	D. melanogaster
Name		FlyBase ID	FBal0035442
Feature type	allele	Associated gene	Dmel\pnt

Map ( GBrowse )	Untitled Document
Allele class	hypomorphic allele - genetic evidence
Mutagen	Delta2-3
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class	hypomorphic allele - genetic evidence (Albagli et al., 1996, Isaksson et al., 1997)
Mutagen	Delta2-3 (Scholz et al., 1993)
Mutations Mapped to the Genome

Type Location Additional Notes References transposable element insertion site 3R:19,171,120..19,171,884 (Brunner et al., 1994, Scholz et al., 1993, Frankfort and Mardon, 2004, Charroux et al., 2006)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Insertion of a P-element. (Brunner et al., 1994) 12kb P-element transposon insertion into the middle of exon 1 at position +365 of the P2 pnt transcript. (Scholz et al., 1993)
Caused by insertion	P{lacW}pnt¹²⁷⁷ (Brunner et al., 1994, Scholz et al., 1993, Frankfort and Mardon, 2004, Charroux et al., 2006)
Cytology
Phenotypic Data
Phenotypic Class
	cell polarity defective (with pnt^Δ88) (Brown and Freeman, 2003) planar polarity defective (with pnt¹²³⁰) (Weber et al., 2008) planar polarity defective (with pnt^Δ88) (Weber et al., 2008) viable (Isaksson et al., 1997, Albagli et al., 1996) visible (with pnt^Δ88) (Rendina et al., 2010)
Phenotype Manifest In
	eye (with pnt^Δ88) (Rendina et al., 2010) eye photoreceptor cell (Albagli et al., 1996) eye photoreceptor cell (with pnt^Δ88) (Rendina et al., 2010) ommatidium (with pnt¹²³⁰) (Weber et al., 2008) ommatidium (with pnt^Δ88) (Brown and Freeman, 2003, Bai et al., 2001, Weber et al., 2008, Fetting et al., 2009) photoreceptor (with pnt^Δ88) (Brown and Freeman, 2003) photoreceptor cell R7 (Albagli et al., 1996) photoreceptor cell R7 (with pnt^Δ88) (Bai et al., 2001)
Detailed Description
	Statement Reference pnt[Δ88]/pnt[1277] flies have rough eyes and show a loss of photoreceptor cells. (Rendina et al., 2010) Many pnt[Δ88]/pnt[1277] ommatidia over- or under-rotate; the variance (s.d.) in degree of rotation is greater than in wild type in adult eyes. (Fetting et al., 2009) pnt[1277]/pnt[1230] eye discs show polarity defects (10% of ommatidial clusters are inverted and 9% are symmetrical). pnt[1277]/pnt[Δ88] eye discs show polarity defects (6.7% of ommatidial clusters are inverted and 6% are symmetrical). (Weber et al., 2008) pnt¹²⁷⁷/pnt^Δ88 flies display a weak rough eye phenotype but pnt¹²⁷⁷/pnt⁰⁷⁸²⁵ flies have normal eyes. (Charroux et al., 2006) In pnt¹²⁷⁷/pnt^Δ88 mutant adults defects are seen in the eye. Under-recruitment of phtoreceptors is common, though in those ommatidia that are correctly specified, mis-rotations are seen. (Brown and Freeman, 2003) pnt¹²⁷⁷/pnt^Δ88 show reduced number of R7 photoreceptors. (Bai et al., 2001) 90% of the ommatidia in pnt^Δ88/pnt¹²⁷⁷ flies lack the R7 photoreceptor and/or some outer photoreceptor cells. (Albagli et al., 1996) Viable. Heterozygotes with pnt^Δ88 lack one to three photoreceptor cells in most ommatidia. The R7 precursor can be rescued by pnt P2 transcript expressed by sev enhancers but the mutant phenotype is enhanced by pnt^{T151A.Scer\UAS} expressed by sev enhancers. (Brunner et al., 1994)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference pnt¹²⁷⁷/pnt^Δ88 has visible phenotype, enhanceable by Scer\GAL4^elav.PU/Bap170^C1.Scer\UAS (Rendina et al., 2010)
Suppressed by
Statement Reference pnt¹²⁷⁷/pnt^Δ88 has visible phenotype, suppressible by Bap170^hfl1/Bap170[+] (Rendina et al., 2010)
Suppressor of
Statement Reference pnt¹²⁷⁷ is a suppressor of visible phenotype of faf^BX4 (Isaksson et al., 1997)
Phenotype Manifest In
Enhanced by
Statement Reference pnt¹²⁷⁷/pnt^Δ88 has eye phenotype, enhanceable by Scer\GAL4^elav.PU/Bap170^C1.Scer\UAS (Rendina et al., 2010) pnt¹²⁷⁷/pnt^Δ88 has eye photoreceptor cell phenotype, enhanceable by Scer\GAL4^elav.PU/Bap170^C1.Scer\UAS (Rendina et al., 2010) pnt¹²⁷⁷/pnt^Δ88 has ommatidium phenotype, enhanceable by ed[+]/ed^1X5 (Fetting et al., 2009)
Suppressed by
Statement Reference pnt¹²⁷⁷/pnt^Δ88 has eye photoreceptor cell phenotype, suppressible by Bap170^hfl1/Bap170[+] (Rendina et al., 2010) pnt¹²⁷⁷/pnt^Δ88 has ommatidium phenotype, suppressible by ed^slH8/ed^1X5 (Bai et al., 2001) pnt¹²⁷⁷/pnt^Δ88 has ommatidium phenotype, suppressible by fred[+]/fred^H24 (Fetting et al., 2009) pnt¹²⁷⁷/pnt^Δ88 has photoreceptor cell R7 phenotype, suppressible by ed^slH8/ed^1X5 (Bai et al., 2001) pnt¹²⁷⁷ has eye photoreceptor cell phenotype, suppressible \| partially by ALV\v-ets^Scer\UAS.cAa/Scer\GAL4^hs.2sev (Albagli et al., 1996) pnt¹²⁷⁷ has eye photoreceptor cell phenotype, suppressible \| partially by Scer\GAL4^hs.2sev/Ggal\ets2^Scer\UAS.cAa (Albagli et al., 1996)
Enhancer of
Statement Reference pnt¹²⁷⁷/pnt⁰⁷⁸²⁵ is an enhancer of ommatidium phenotype of Gug^Scer\UAS.cCa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Charroux et al., 2006) pnt¹²⁷⁷/pnt^Δ88 is an enhancer of interommatidial bristle phenotype of Gug^Scer\UAS.cCa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Charroux et al., 2006)
Suppressor of
Statement Reference pnt¹²⁷⁷ is a suppressor of phenotype of Jra^Asp.hs.sev (Treier et al., 1995) pnt¹²⁷⁷ is a suppressor of photoreceptor cell R1 \| ectopic phenotype of faf^BX4 (Isaksson et al., 1997) pnt¹²⁷⁷ is a suppressor of photoreceptor cell R2 \| ectopic phenotype of faf^BX4 (Isaksson et al., 1997) pnt¹²⁷⁷ is a suppressor of photoreceptor cell R3 \| ectopic phenotype of faf^BX4 (Isaksson et al., 1997) pnt¹²⁷⁷ is a suppressor of photoreceptor cell R4 \| ectopic phenotype of faf^BX4 (Isaksson et al., 1997) pnt¹²⁷⁷ is a suppressor of photoreceptor cell R5 \| ectopic phenotype of faf^BX4 (Isaksson et al., 1997) pnt¹²⁷⁷ is a suppressor of photoreceptor cell R6 \| ectopic phenotype of faf^BX4 (Isaksson et al., 1997)
Additional Comments
Genetic Interactions
Statement Reference Bap170[hfl1]/+ suppresses the loss of photoreceptor cells seen in pnt[Δ88]/pnt[1277] flies. Expression of Bap170[C1.Scer\UAS] under the control of Scer\GAL4[elav.PU] enhances the rough eye phenotype and loss of photoreceptor cells seen in pnt[Δ88]/pnt[1277] flies. (Rendina et al., 2010) ed[1X5] dominantly enhances the phenotype of mis-rotation of ommatidia that is seen in pnt[Δ88]/pnt[1277] animals. fred[H24] dominantly suppresses the phenotype of mis-rotation of ommatidia that is seen in pnt[Δ88]/pnt[1277] animals. (Fetting et al., 2009) The rough eye phenotype of flies expressing Gug^Scer\UAS.cCa under the control of Scer\GAL4^GMR.PF is enhanced in both a pnt¹²⁷⁷/pnt^Δ88 and a pnt¹²⁷⁷/pnt⁰⁷⁸²⁵ background. (Charroux et al., 2006) The extra outer photoreceptor cell phenotype seen in faf^BX4 homozygotes is suppressed by pnt¹²⁷⁷, with the average number of outer photoreceptor cells per ommatidium being reduced to 6.2. (Isaksson et al., 1997) pnt^Δ88/pnt¹²⁷⁷ or pnt¹²³⁰/pnt¹²⁷⁷ completely suppresses the ectopic R7 rough eye phenotype of Jra^Asp.hs.sev. (Treier et al., 1995)
Xenogenetic Interactions
Statement Reference The eye phenotype of pnt^Δ88/pnt¹²⁷⁷ flies is partially rescued when Ggal\ets2^Scer\UAS.cAa or ALV\v-ets^Scer\UAS.cAa is expressed using Scer\GAL4^hs.2sev. (Albagli et al., 1996)
Complementation & Rescue Data
Partially rescued by	pnt¹²⁷⁷/pnt^Δ88 is partially rescued by Scer\GAL4^hs.2sev/pnt^P1.Scer\UAS (Albagli et al., 1996)
Comments	The eye phenotype of pnt^Δ88/pnt¹²⁷⁷ flies is partially rescued when pnt^P1.Scer\UAS is expressed using Scer\GAL4^hs.2sev at 17^oC. (Albagli et al., 1996) The R7 precursor can be rescued by pnt P2 transcript expressed by sev enhancers but the mutant phenotype is enhanced by pnt^{T151A.Scer\UAS} expressed by sev enhancers. (Brunner et al., 1994)
Stocks ( 1 )
Bloomington	837 w¹¹¹⁸; P{lacW}pnt¹²⁷⁷
Notes on Origin
Discoverer	Beermann.

Comments
	Weak P2-specific pnt mutant. (Brunner et al., 1994) Allelic series of pnt allele that affect the P2 transcript: pnt¹²⁷⁷ < pnt¹²⁰² < pnt¹²⁰⁸ < pnt¹²³⁰ <= pnt^T9 < pnt^T5. (Scholz et al., 1993)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	pnt¹²⁷⁷ (Weber et al., 2008, Fetting et al., 2009, Rendina et al., 2010)
Name Synonym
Secondary FlyBase IDs

References ( 13 )
Research paper	Rendina et al., 2010, Genetics 186(1): 167--181 Bap170, a Subunit of the Drosophila PBAP Chromatin Remodeling Complex, Negatively Regulates the EGFR Signaling. [FBrf0211845] Fetting et al., 2009, Development 136(19): 3323--3333 The cell adhesion molecules Echinoid and Friend of Echinoid coordinate cell adhesion and cell signaling to regulate the fidelity of ommatidial rotation in the Drosophila eye. [FBrf0208836] Weber et al., 2008, Dev. Biol. 316(1): 110--123 Combinatorial signaling by the Frizzled/PCP and Egfr pathways during planar cell polarity establishment in the Drosophila eye. [FBrf0204109] Charroux et al., 2006, Dev. Biol. 291(2): 278--290 Atrophin contributes to the negative regulation of epidermal growth factor receptor signaling in Drosophila. [FBrf0190100] Frankfort and Mardon, 2004, Development 131(3): 563--570 Senseless represses nuclear transduction of Egfr pathway activation. [FBrf0167533] Brown and Freeman, 2003, Development 130(22): 5401--5412 Egfr signalling defines a protective function for ommatidial orientation in the Drosophila eye. [FBrf0167498] Bai et al., 2001, Development 128(4): 591--601 The cell adhesion molecule Echinoid defines a new pathway that antagonizes the Drosophila EGF receptor signaling pathway. [FBrf0134572] Isaksson et al., 1997, Mech. Dev. 68(1,2): 59--67 The deubiquitination enzyme fat facets negatively regulates RTK/Ras/MAPK signalling during Drosophila eye development. [FBrf0099843] Albagli et al., 1996, Mech. Dev. 59(1): 29--40 Function of ets genes is conserved between vertebrates and Drosophila. [FBrf0090431] Treier et al., 1995, Cell 83(5): 753--760 JUN cooperates with the ETS domain protein pointed to induce photoreceptor R7 fate in the Drosophila eye. [FBrf0086055] Brunner et al., 1994, Nature 370(6488): 386--389 The ETS domain protein pointed-P2 is a target of MAP kinase in the sevenless signal transduction pathway. [FBrf0072711] Klaes et al., 1994, Cell 78(1): 149--160 The Ets transcription factors encoded by the Drosophila gene pointed direct glial cell differentiation in the embryonic CNS. [FBrf0073570] Scholz et al., 1993, Genetics 135(2): 455--468 Genetic dissection of pointed, a Drosophila gene encoding two ETS-related proteins. [FBrf0064691]

Allele Dmel\pnt1277

Allele Dmel\pnt¹²⁷⁷