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General Information
Symbol
Dmel\pntP1.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0035443
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-PNTP1, UAS-pnt.P1, UAS-pntP1, UAS-pnt-P1
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference
UASt regulatory sequences drive expression of the pnt P1 isoform (the entire open reading frame plus 500bp of 5'UTR and 260bp of 3'UTR sequence).
Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
The expression of pntP1.Scer\UAS under the control of Scer\GAL4prd.PU leads to extra and ectopic oenocytes in Prd-positive abdominal and thoracic segments, respectively, during stage 15 of embryogenesis, as compared to controls; expression under the control of Scer\GAL4salm-459.2, but not Scer\GAL4ato.3.6, also leads to extra oenocytes in abdominal segments, as compared to controls.
Expression of pntP1.Scer\UAS under the control of either Scer\GAL4esg-NP5130 or Scer\GAL4Dl.PU (using tub-Gal80[ts] to limit the time of the transgene expression) results in intestinal stem cell overproliferation in the adult midgut.
The short lived phenotype seen in flies expressing pntP1.Scer\UAS under the control of Scer\GAL4da.Switch.PT is responsive to dietary yeast. trametinib treatment at 15.6uM has no effect on the shortened lifespan seen in flies expressing Ras85DV12.cUa.Scer\UAS under the control of Scer\GAL4da.Switch.PT.
Expression of pntP1.Scer\UAS in the adult gut and fat body under the control of Scer\GAL4Switch1.106 (in the presence of RU486) has a marginal negative effect on lifespan compared to controls. Expression of pntP1.Scer\UAS in the adult gut under the control of Scer\GAL4TIGS-2 (in the presence of RU486) has detrimental effect on lifespan compared to controls. Stored triacylglycerol (TAG) levels are also reduced compared to controls. Gut integrity and feeding appear normal.
Expression of pntP1.Scer\UAS driven by Scer\GAL4ey.PU causes a dramatic reduction in the size of the adult eye.
Scer\GAL4btl.PS-mediated expression of pntP1.Scer\UAS disrupts tracheal branching in the embryo.
Expression of pntP1.Scer\UAS under the control of Scer\GAL41151 results in an increased number of founder cells compared to the wild-type number in the abdomen.
pntP1.Scer\UAS; Scer\GAL4sca-T3 adult ommatidia do not contain small rhabdomeres, suggesting an absence of R8 photoreceptors. Ommatidia in these eyes also contain a variable number of photoreceptors.
When expression is driven by Scer\GAL4He.PZ, hemocyte proliferation is increased and lamellocyte and crystal cells numbers are unaffected. Melanotic masses form. Similar, though milder, effects are seen when expression is driven by Scer\GAL4Hml.PG.
Expression of pntP1.Scer\UAS driven by Scer\GAL4twi.PG, leads to a near complete loss of pericardial and cardioblast cells.
Expression of pntP1.Scer\UAS under the control of Scer\GAL4ey.PB can result in homeotic transformation of the eye to antenna.
Expression of pntP1.Scer\UAS under the control of Scer\GAL4dpp.blk1 results in ectopic furrow formation in the eye disc.
Expression of pntP1.Scer\UAS under the control of Scer\GAL4nos.PG results in embryos with defects in the patterning the head skeleton.
In pntP1.Scer\UAS/Scer\GAL471B wings the intervein material between L2 and L3 is lost and wing vein L4 is reduced. If pntP1.Scer\UAS is driven by Scer\GAL4dpp.blk1 increased numbers of macrochaetae are seen on the scutellum. The wings are small and bifurcated. In the wing disc a central cleft is seen.
Scer\GAL4sim.PS-mediated expression causes an increase in number of midline glial cells.
Produces pupal lethality when expressed using Scer\GAL4hs.2sev at 25oC. At 17oC, flies expressing pntP1.Scer\UAS using Scer\GAL4hs.2sev eclose, and have rough eyes. The number of rhabdomeres per ommatidium is normal, but ommatidial morphology and orientation is abnormal. Pigment cells are missing between several ommatidia. This phenotype is more pronounced in the posterior part of the eye.
Scer\GAL455B and Scer\GAL4c324a mediated expression produces female progeny that are sterile or semi-sterile and produce eggs that have dorsal defects (no appendages or small 'nubs' of appendage material, 'crumpled' chorion material). Overexpression results in loss of dorsal follicle cells.
With either Scer\GAL4sca-537.4 or Scer\GAL4rho.PL, there is a reduction or complete lack of commissural connectives. At stages 12-14 ectopic glial cells are evident in the lateral CNS in embryos with Scer\GAL4sca-537.4. These ectopic glial cells are flanked by neurons ectopically expressing the neural antigen 22C10.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
The increased/ectopic oenocytes in Prd-positive abdominal segments during stage 15 of embryogenesis observed upon the expression of pntP1.Scer\UAS under the control of Scer\GAL4prd.PU is suppressed by the co-expression of EgfrDN.cUa.Scer\UAS, even leading to the complete absence of oenocytes.
The increase in the adult midgut intestinal stem cell proliferation characteristic for flies expressing pntP1.Scer\UAS under the control of Scer\GAL4esg-NP5130 (using tub-Gal80[ts] to limit the time of the transgene expression) is not suppressed by co-expression of cicΔC2.Scer\UAS.T:Ivir\HA1.
Expression of foxoScer\UAS.cFa enhances the reduction in lifespan seen when pntP1.Scer\UAS is expressed in the adult midgut and fat body under the control of Scer\GAL4Switch1.106 (in the presence of RU486). This phenotype is completely suppressed upon expression of aopACT.Scer\UAS. Co-expression of foxoScer\UAS.cFa and pntP1.Scer\UAS also enhances the reduction in stored triacylglycerol (TAG) levels seen when either transgene is expressed alone. Gut integrity and feeding appear normal. Expression of foxoScer\UAS.cFa enhances the reduction in lifespan seen when pntP1.Scer\UAS is expressed in the adult midgut under the control of Scer\GAL4TIGS-2 (in the presence of RU486). This phenotype is partially suppressed upon expression of aopACT.Scer\UAS. Flies co-expressing pntP1.Scer\UAS and foxoScer\UAS.cFa under the control of Scer\GAL4Switch1.106 are starvation sensitive after 5 days in the presence of RU486, and this phenotype can be reversed upon expression of aopACT.Scer\UAS.
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Partially rescues
Fails to rescue
Comments
Partially rescues the eye phenotype of pntΔ88/pnt1277 flies when expressed using Scer\GAL4hs.2sev at 17oC.
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
pntP1.Scer\UAS
pntP1.UAS
Name Synonyms
Secondary FlyBase IDs
    References (51)