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General Information
Symbol
Dmel\pnutXP
Species
D. melanogaster
Name
FlyBase ID
FBal0035461
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Deletion of at least 17kb that lacks nearly all of the pnut coding region.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

pnutXP mutant larvae lack imaginal discs and show polyploidy metaphases in neural ganglia. pnutXP mutant larvae also carrying pnutK155A.D206A.K288A.pnut.T:Zzzz\FLAG show an indistinguishable phenotype from that of pnutXP single mutants.

pnutXP flies in which pnuths.PN is expressed using daily heat shocks during larval and pupal development survive to adulthood. Female flies efficiently produce eggs. However, in most crosses of these females to either wild-type or pnutrN498/+ males, all of the embryos die without hatching, even when heat shocks are continued for 1 to 5 days after eclosion of the adult flies. Events prior to gastrulation appear to occur normally in embryos derived from a cross of pnutXP females carrying pnuths.PN (and rescued to adulthood by daily heat shock) with either wild-type or pnutXP/+ males; a layer of cleared cytoplasm forms at the cortex and cellularisation begins and proceeds evenly over the entire embryo at a normal speed and depth, although the cellularisation front is typically somewhat less well defined than in wild-type embryos. Global defects are generally not seen until the onset of gastrulation, when an ectopic fold is often seen anterior to the cephalic furrow. In addition, the posterior midgut plate often migrates to the left or right of the embryo instead of dorsally.

Dissection of the third instar larvae revealed severely reduced imaginal discs. Mutant brains contain a large number of polyploid cells. Most often these cells are tetraploid, but a poidy of 8 or more has been observed. Nucler to cytoplasm ratio measurements indicated a failure in cytokinesis. Large multinucleate cells were found in all imaginal tissues examined, with most severe effects in the lymph glands and imaginal rings of the salivary glands, in which nearly all cells contain multiple nuclei.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhancer of
Statement
Reference
Suppressor of
Statement
Reference

pnut[+]/pnutXP is a suppressor of visible phenotype of rprGMR.PW

pnut[+]/pnutXP is a suppressor | partially of visible phenotype of hidGMR.PG/WGMR.PG

pnut[+]/pnutXP is a suppressor of visible phenotype of grimGMR.PC

Phenotype Manifest In
Enhancer of
Statement
Reference

pnutXP is an enhancer of ommatidium phenotype of S48-5

NOT Enhancer of
Statement
Reference

pnut[+]/pnutXP is a non-enhancer of mitotic domain 1 | embryonic cycle 14 phenotype of CycB+t10

Suppressor of
Statement
Reference

pnut[+]/pnutXP is a suppressor of eye phenotype of rprGMR.PW

pnut[+]/pnutXP is a suppressor | partially of eye phenotype of hidGMR.PG/WGMR.PG

pnut[+]/pnutXP is a suppressor of eye phenotype of grimGMR.PC

NOT Suppressor of
Statement
Reference

pnut[+]/pnutXP is a non-suppressor of mitotic domain 1 | embryonic cycle 14 phenotype of CycB+t10

Additional Comments
Genetic Interactions
Statement
Reference

pnutXP/+ significantly suppresses the reduced eye phenotype caused by expression of grimGMR.PC, WGMR.PG or rprGMR.PW.

S48-5/pnutXP mutants show 24.58%+-5.90 misrotated ommatidia compared to 9.55%+-1.43 seen in S48-5 mutants alone.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Fails to complement
Partially rescued by
Comments

pnutXP fails to complement the lethality of pnutmut1 mutants.

pnut1-427.pnut.T:Zzzz\FLAG fails to rescue the pnutXP third-instar lethal phenotype. However, developing imaginal discs are found in such larvae, as compared with the disc-less phenotype of pnutXP larvae.

pnutXP mutant larvae also carrying pnutK155A.D206A.K288A.pnut.T:Zzzz\FLAG show an indistinguishable phenotype from that of pnutXP single mutants.

pnutK288A.pnut.T:Zzzz\FLAG fails to rescue the lethality of pnutXP. However, the cytokinesis defects associated with pnutXP are rescued. Imaginal disc development is also rescued by pnutK288A.pnut.T:Zzzz\FLAG.

Lethality can be rescued by P{pnut} or P{hs-pnut}.

Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (7)
References (14)