Allele Dmel\pnut^rN498

General Information
Symbol	Dmel\pnut^rN498	Species	D. melanogaster
Name		FlyBase ID	FBal0035463
Feature type	allele	Associated gene	Dmel\pnut

Map ( GBrowse )	Untitled Document
Allele class
Mutagen	P-element activity
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	P-element activity (Neufeld and Rubin, 1994)
Mutations Mapped to the Genome

Type Location Additional Notes References transposable element insertion site 2R:4,125,628..4,125,629 (BDGP Project Members, 1994-1999, Neufeld and Rubin, 1994, Spradling et al., 1999, Adam et al., 2000, Fares et al., 1995)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference P{PZ} insertion at nucleotide 75 of the pnut coding region. (Adam et al., 2000) Insertion of PZ element into the 5' end of the pnut transcription unit. (Neufeld and Rubin, 1994)
Caused by insertion	P{PZ}pnut^rN498 (BDGP Project Members, 1994-1999, Neufeld and Rubin, 1994, Spradling et al., 1999, Adam et al., 2000, Fares et al., 1995)
Cytology
Phenotypic Data
Phenotypic Class
	lethal \| embryonic stage \| germline clone \| non-rescuable maternal effect \| recessive (Adam et al., 2000) lethal \| recessive (Spradling et al., 1999, BDGP Project Members, 1994-1999)
Phenotype Manifest In
	actin filament \| embryonic stage \| germline clone (Adam et al., 2000) denticle belt \| germline clone (Adam et al., 2000) egg \| germline clone (Adam et al., 2000) embryonic/first instar larval cuticle \| germline clone (Adam et al., 2000) embryonic head \| germline clone (Adam et al., 2000) germarium region 1 \| germline clone (Adam et al., 2000) nucleus \| embryonic stage \| germline clone (Adam et al., 2000) ventral furrow & actin filament \| germ-line clone (Adam et al., 2000)
Detailed Description
	Statement Reference Females containing homozygous germ line clones efficiently produce eggs for at least 10 days after eclosion. The eggs are approximately 70% the length of wild-type eggs and are abnormally round. Some stem cells are still dividing actively in 4 day old females containing homozygous germ line clones. The morphology of the germarium is often abnormal in these flies, with region 1 being shorter than normal. Embryos derived from females containing homozygous germ line clones crossed to either wild-type or pnut^rN498/+ males die without hatching. The embryos have a characteristic but variable cuticle phenotype. The cuticles are always smaller than wild type and usually do not fill the eggshell. The head and tail structures are severely defective. Denticles can be identified and are usually grouped in clusters or bands, but the number of bands varies in number (from 0 to 5, with an average of about 3), shape, size and position. The types and severities of the cuticle phenotype are similar in embryos derived from crosses of females containing homozygous germ line clones to either wild-type or pnut^rN498/+ males. Actin organisation prior to cellularisation appears approximately normal in embryos derived from females containing homozygous germ line clones. The actin cytoskeleton appears normal at the beginning of cellularisation and through the slow phase of cellularisation. However, defects in the organisation of actin at the bases of forming cells are seen during the fast phase of cellularisation, although most embryos appear grossly normal at this stage. The actin does not resolve into the discrete rings seen in wild-type embryos and is more uniformly distributed in the plane of the leading edge, except for the presence of concentrated "bars" of actin between some pairs of cell bases. Approximately 10% of the embryos show regions where the bases of cells are unevenly closed. In these embryos, there is an associated disruption of the actin cytoskeleton along the lateral surfaces of the forming cells and occasional abnormal displacement of nuclei from the cortex. The bases of many cells appear to reopen at the beginning of gastrulation, the distribution of actin along the cellularization front becomes more patchy than in wild-type embryos, cell integrity is disrupted over large parts of the embryo and germ band extension is delayed. In slightly older embryos, nuclei that are no longer surrounded by an actin cytoskeleton are seen and small areas at the anterior of the embryo seem to be devoid of nuclei. The actin cytoskeleton at the ventral furrow is grossly disorganised. (Adam et al., 2000)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Fails to complement	pnut⁰²⁵⁰² (BDGP Project Members, 1994-1999) pnut^rQ348 (BDGP Project Members, 1994-1999)
Rescued by	pnut^rN498 is rescued by pnut^+t10 (Adam et al., 2000)
Comments
Stocks ( 0 )

Notes on Origin
Discoverer	G. Rubin.

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	l(2)02502^rN498 l(2)rN498 (Spradling et al., 1999) pnut^rN498
Name Synonym
Secondary FlyBase IDs
	FBal0043416
References ( 6 )
Research paper	Adam et al., 2000, Mol. Biol. Cell 11(9): 3123--3135 Evidence for functional differentiation among Drosophila septins in cytokinesis and cellularization. [FBrf0129694] Spradling et al., 1999, Genetics 153(1): 135--177 The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489] Fares et al., 1995, Mol. Biol. Cell 6(12): 1843--1859 Localization and possible functions of Drosophila septins. [FBrf0085016] Neufeld and Rubin, 1994, Cell 77(3): 371--379 The Drosophila peanut gene is required for cytokinesis and encodes a protein similar to yeast putative bud neck filament proteins. [FBrf0074019]
Personal communication to FlyBase	Beaton, 1999.12.12, Alleles of the lines in the P-element paper. Alleles of the lines in the P-element paper. [FBrf0125032] BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]

Allele Dmel\pnutrN498

Allele Dmel\pnut^rN498