Allele Dmel\ptc^H84

General Information
Symbol	Dmel\ptc^H84	Species	D. melanogaster
Name		FlyBase ID	FBal0035473
Feature type	allele	Associated gene	Dmel\ptc

Allele class	amorphic allele - genetic evidence
Mutagen	P-element activity
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class	amorphic allele - genetic evidence (Hooper, 1994, Bhat, 1996)
Mutagen	P-element activity (Ingham et al., 1991, Hooper, 1994, Zhang et al., 1996)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference P{lacZ} has inserted into the 3' exon of ptc. (Alcedo et al., 1996)
Caused by insertion	P{A92}ptc^H84 (Ingham et al., 1991, Thomas and Ingham, 2003, Hooper, 1994, Alcedo et al., 1996, Zhang et al., 1996, Bhat, 1996)
Cytology
Phenotypic Data
Phenotypic Class
	lethal (Zhang et al., 1996) visible (with ptc⁹) (Xia et al., 2012)
Phenotype Manifest In
	EL neuron (Deshpande et al., 2001) eye (with ptc^tuf-1) (Thomas and Ingham, 2003) eye-antennal disc (with ptc^hdl) (Shyamala and Bhat, 2002) frons (with ptc^tuf-1) (Thomas and Ingham, 2003) fronto-orbital plate (with ptc^tuf-1) (Thomas and Ingham, 2003) head bristle (with ptc^559.1) (Thomas and Ingham, 2003) head bristle (with ptc^tuf-1) (Thomas and Ingham, 2003) head bristle \| ectopic (with ptc^559.1) (Thomas and Ingham, 2003) head bristle \| ectopic (with ptc^tuf-1) (Thomas and Ingham, 2003) head capsule (with ptc^559.1) (Shyamala and Bhat, 2002) head capsule (with ptc^hdl) (Shyamala and Bhat, 2002) neuroblast NB4-2 (Bhat, 1996) neuroblast NB7-3 \| ectopic (Deshpande et al., 2001) ocellar triangle (with ptc^tuf-1) (Thomas and Ingham, 2003) wing (with ptc⁹) (Xia et al., 2012)
Detailed Description
	Statement Reference ptc^tuf-1/ptc^H84 flies have mildly reduced eyes but a highly enlarged head vertex. 90% of these flies have outgrowths of head cuticle and large numbers of missing, misplaced or ectopic head bristles with the remaining 10% having no outgrowths and one or 2 displaced or missing bristles. ptc^559.1/ptc^H84 flies have normal sized eyes and head vertex and a low incidence of ectopic or misplaced head bristles. (Thomas and Ingham, 2003) 100% of ptc^hdl embryos transheterozygous with ptc^H84 show head capsule defects. 4% of ptc^H84/ptc^559.1 transheterozygotes show head capsule defects. (Shyamala and Bhat, 2002) An NB 7-3-like fate is formed ectopically in 50% of mutant hemineuromeres. 60% of EL cells are missing. (Deshpande et al., 2001) 30% hemisegments exhibit failure of neuroblast NB4-2 to delaminate from the neuroectoderm. (Bhat, 1996)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference ptc^H84/ptc⁹ has visible phenotype, enhanceable by Ubpy^{Scer\UAS.T:Zzzz\FLAG}/Scer\GAL4^Bx-MS1096 (Xia et al., 2012)
Phenotype Manifest In
Enhanced by
Statement Reference ptc^559.1/ptc^H84 has head capsule phenotype, enhanceable by smo¹ (Shyamala and Bhat, 2002) ptc^H84/ptc⁹ has wing phenotype, enhanceable by Ubpy^{Scer\UAS.T:Zzzz\FLAG}/Scer\GAL4^Bx-MS1096 (Xia et al., 2012)
Suppressed by
Statement Reference ptc^H84 has neuroblast NB4-2 phenotype, suppressible by Df(2R)gsb (Bhat, 1996) ptc^hdl/ptc^H84 has head capsule phenotype, suppressible by babo^{Q302D.Scer\UAS}/Scer\GAL4^69B (Shyamala and Bhat, 2002)
NOT suppressed by
Statement Reference ptc^hdl/ptc^H84 has head capsule phenotype, non-suppressible by Df(3L)H99 (Shyamala and Bhat, 2002) ptc^hdl/ptc^H84 has head capsule phenotype, non-suppressible by smo¹ (Shyamala and Bhat, 2002)
Other
Statement Reference babo³², ptc^H84 has head capsule phenotype (Shyamala and Bhat, 2002, Shyamala and Bhat, 2002) babo^k07737, ptc^H84, smo¹/smo[+] has head capsule phenotype (Shyamala and Bhat, 2002) babo^k07737, ptc^H84, smo¹ has head capsule phenotype (Shyamala and Bhat, 2002, Shyamala and Bhat, 2002) ptc^H84, smo¹ has head capsule phenotype (Shyamala and Bhat, 2002, Shyamala and Bhat, 2002)
Additional Comments
Genetic Interactions
Statement Reference The addition of smo¹ to ptc^H84/ptc^559.1 animals produces an enhancement of the head capsule defect phenotype. 35% of animals exhibit the phenotype, compared to 4%. The addition of smo¹ to ptc^H84/ptc^hdl animals has no effect on the head capsule defect phenotype. All animals continue to show the phenotype. 2% of ptc^H84, smo¹/+ animals show a head capsule defect. 5% of babo³², ptc^H84 double heterozygotes exhibit head capsule defects. 10% of ptc^H84, smo¹/+, babo^k07737 animals exhibit head capsule defects. No ptc^H84, put¹⁰⁴⁶⁰ animals exhibit head capsule defects. The addition of Df(3L)H99 of ptc^H84/ptc^hdl does not suppress head capsule defects. The addition of babo^{Q302D.Scer\UAS} (driven by Scer\GAL4^69B) suppresses the head capsule defect phenotype seen in ptc^H84/ptc^hdl. None of these animals exhibit head capsule defects. (Shyamala and Bhat, 2002) Simultaneous elimination of gsb, Df(2R)gsb, rescues the RP2 lineage in 70% of hemisegments. (Bhat, 1996)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Fails to complement	ptc^k02507 (Prokopenko et al., 2000)
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	ptc^H84 (Merianda et al., 2005, Xia et al., 2012) ptc^H84lacZ (Thomas and Ingham, 2003) unnamed (Ingham et al., 1991)
Name Synonym
Secondary FlyBase IDs

References ( 11 )
Research paper	Xia et al., 2012, PLoS Biol. 10(1): e1001238 USP8 promotes smoothened signaling by preventing its ubiquitination and changing its subcellular localization. [FBrf0218247] Merianda et al., 2005, Dev. Genes Evol. 215(6): 285--296 Patched regulation of axon guidance is by specifying neural identity in the Drosophila nerve cord. [FBrf0187440] Thomas and Ingham, 2003, Genetics 165(4): 1915--1928 Hedgehog signaling in the Drosophila eye and head: an analysis of the effects of different patched trans-heterozygotes. [FBrf0167651] Shyamala and Bhat, 2002, Development 129(8): 1839--1847 A positive role for Patched-Smoothened signaling in promoting cell proliferation during normal head development in Drosophila. [FBrf0146982] Deshpande et al., 2001, Development 128(17): 3253--3261 Successive specification of Drosophila neuroblasts NB 6-4 and NB 7-3 depends on interaction of the segment polarity genes wingless, gooseberry and naked cuticle. [FBrf0138370] Prokopenko et al., 2000, Genetics 156(4): 1691--1715 Mutations affecting the development of the peripheral nervous system in Drosophila: A molecular screen for novel proteins. [FBrf0131381] Alcedo et al., 1996, Cell 86(2): 221--232 The Drosophila smoothened gene encodes a seven-pass membrane protein, a putative receptor for the hedgehog signal. [FBrf0089582] Bhat, 1996, Development 122(9): 2921--2932 The patched signaling pathway mediates repression of gooseberry allowing neuroblast specification by wingless during Drosophila neurogenesis. [FBrf0090453] Zhang et al., 1996, Genetics 143(3): 1231--1241 Identification and genetic analysis of wunen, a gene guiding Drosophila melanogaster germ cell migration. [FBrf0089219] Ingham et al., 1991, Nature 353: 184--187 Role of the Drosophila patched gene in positional signalling. [FBrf0054767]
Letter	Hooper, 1994, Nature 372(6505): 461--464 Distinct pathways for autocrine and paracrine Wingless signalling in Drosophila embryos. [FBrf0076991]

Allele Dmel\ptcH84

Allele Dmel\ptc^H84