Allele Dmel\rhi⁰²⁰⁸⁶

General Information
Symbol	Dmel\rhi⁰²⁰⁸⁶	Species	D. melanogaster
Name		FlyBase ID	FBal0035505
Feature type	allele	Associated gene	Dmel\rhi
Also Known As	rhi²

Allele class
Mutagen	P-element activity
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	P-element activity (Volpe and Berg, 1995)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference P{PZ} insertion at nucleotide 267. The P{PZ} element is oriented in the opposite direction to the direction of rhi transcription. (Volpe et al., 2001)
Caused by insertion	P{PZ}rhi⁰²⁰⁸⁶ (Volpe and Berg, 1995, Spradling et al., 1999, Volpe et al., 2001)
Cytology
Phenotypic Data
Phenotypic Class
	female sterile \| recessive (Spradling et al., 1999)
Phenotype Manifest In
	dorsal appendage (Volpe and Berg, 1995, Volpe et al., 2001) dorsal appendage (with rhi^2-S18) (Volpe et al., 2001) dorsal appendage \| maternal effect (with rhi^KG00910) (Klattenhoff et al., 2009) egg (Volpe and Berg, 1995) egg chamber (with rhi^2-L12) (Volpe et al., 2001) nurse cell (with rhi^2-L12) (Volpe et al., 2001) nurse cell & chromatin (with rhi^2-L12) (Volpe et al., 2001)
Detailed Description
	Statement Reference Only 17% of eggs derived from rhi[KG00910]/rhi[02086] females have two wild-type dorsal appendages. 88% of embryos derived from these females have wild-type appendages if the females are fed caffeine. (Klattenhoff et al., 2009) 50% of eggs laid by homozygous females are wild type, others have dorsal appendage defects. A weak "dumpless" phenotype, in which the nurse cells fail to transfer their contents to the oocyte is also seen in 11% of cases. 15% of eggs laid by rhi^2-S18/rhi⁰²⁰⁸⁶ females are wild type, others have dorsal appendage defects. rhi⁰²⁰⁸⁶/rhi^2-L12 egg chambers have normal nurse cell chromatin morphology up to stage S5, but only 11% have normal nurse cell chromatin morphology at other stages. 58% of the egg chambers have a persistant five-lobed chromatin phenotype. 7% of egg chambers are degenerating and 4% have an abnormal number of nurse cells. (Volpe et al., 2001) Eggs laid are shorter than wild type and the dorsal appendages are fused. (Volpe and Berg, 1995)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Suppressed by
Statement Reference rhi⁰²⁰⁸⁶/rhi^KG00910 has dorsal appendage \| maternal effect phenotype, suppressible \| partially by lok^p6 (Klattenhoff et al., 2009) rhi⁰²⁰⁸⁶/rhi^KG00910 has dorsal appendage \| maternal effect phenotype, suppressible \| partially by mei-41^D3 (Klattenhoff et al., 2009)
Additional Comments
Genetic Interactions
Statement Reference 80% of eggs derived from lok[p6] ; rhi[KG00910]/rhi[02086] double mutant females have two normal dorsal appendages. 33% of eggs derived from mei-41[D3] ; rhi[KG00910]/rhi[02086] double mutant females have two normal dorsal appendages. (Klattenhoff et al., 2009)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 1 )
Bloomington	12226 P{PZ}rhi⁰²⁰⁸⁶ cn¹/CyO; ry⁵⁰⁶
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 4 )
Reported As
Symbol Synonym	fs(2)02086 (Spradling et al., 1999) rhi² (Volpe et al., 2001, Vermaak et al., 2005, Klattenhoff et al., 2009) rhi⁰²⁰⁸⁶ (Pane et al., 2011, Neuman-Silberberg, 2007, Klattenhoff et al., 2009) rhi^PZ2
Name Synonym
Secondary FlyBase IDs

References ( 9 )
Research paper	Pane et al., 2011, EMBO J. 30(22): 4601--4615 The Cutoff protein regulates piRNA cluster expression and piRNA production in the Drosophila germline. [FBrf0217070] Klattenhoff et al., 2009, Cell 138(6): 1137--1149 The Drosophila HP1 homolog Rhino is required for transposon silencing and piRNA production by dual-strand clusters. [FBrf0208800] Neuman-Silberberg, 2007, genesis 45(6): 369--381 Drosophila female sterile mutation spoonbill interferes with multiple pathways in oogenesis. [FBrf0201515] Vermaak et al., 2005, PLoS Genet. 1(1): e9 Positive Selection Drives the Evolution of rhino, a Member of the Heterochromatin Protein 1 Family in Drosophila. [FBrf0188223] Volpe et al., 2001, Genetics 159(3): 1117--1134 Drosophila rhino encodes a female-specific chromo-domain protein that affects chromosome structure and egg polarity. [FBrf0141550] Spradling et al., 1999, Genetics 153(1): 135--177 The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489]
Personal communication to FlyBase	Deal-Herr and Cook, 2003.12.11, Isolation and Characterization of Df(2R)BSC45. Isolation and Characterization of Df(2R)BSC45. [FBrf0173243]
Abstract	Volpe and Berg, 1996, A. Dros. Res. Conf. 37: 236 rhino is a female sterile, chromobox-containing gene required for proper dorsal/ventral patterning and chromatin condensation in the egg. [FBrf0086094] Volpe and Berg, 1995, A. Dros. Res. Conf. 36: 132A rhino: a female sterile gene required for dorsal/ventral patterning in the egg. [FBrf0079577]

Allele Dmel\rhi02086

Allele Dmel\rhi⁰²⁰⁸⁶