Allele Dmel\aos^rlt

General Information
Symbol	Dmel\aos^rlt	Species	D. melanogaster
Name		FlyBase ID	FBal0035517
Feature type	allele	Associated gene	Dmel\aos
Also Known As	rlt¹, argos^rlt

Allele class
Mutagen
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Contains a truncated P-element in the 5' UTR of argos within the proposed transcriptional start at bp 55. (Gaengel and Mlodzik, 2003)
Cytology
Phenotypic Data
Phenotypic Class
	cell polarity defective (Gaengel and Mlodzik, 2003, Strutt and Strutt, 2003) cell polarity defective (with aos^5F4) (Strutt and Strutt, 2003) cell polarity defective (with aos^Δ7) (Gaengel and Mlodzik, 2003, Strutt and Strutt, 2003)
Phenotype Manifest In
	eye (Choi and Benzer, 1994) eye photoreceptor cell (Choi and Benzer, 1994) eye photoreceptor cell \| ectopic (Strutt and Strutt, 2003) eye photoreceptor cell \| ectopic (with aos^5F4) (Strutt and Strutt, 2003) eye photoreceptor cell \| ectopic (with aos^Δ7) (Strutt and Strutt, 2003) ommatidium (Gaengel and Mlodzik, 2003, Strutt and Strutt, 2003, Fetting et al., 2009) ommatidium (with aos^5F4) (Strutt and Strutt, 2003) ommatidium (with aos^Δ7) (Gaengel and Mlodzik, 2003, Strutt and Strutt, 2003)
Detailed Description
	Statement Reference Many argos[rlt] ommatidia mis-rotate; the variance (s.d.) in degree of rotation is greater than in wild type in adult eyes and the mean angle of orientation of the ommatidia is only 77.84[o] (compared to 90[o] in wild type). (Fetting et al., 2009) Homozygotes, argos^rlt/argos^Δ7 or argos^rlt/Df(3L)st7P animals exhibit a ommatidial rotation phenotype. Clusters are seen that are both over and under-rotated with respect to their neighbours and developmental stage. Mutant animals do not exhibit any photoreceptor R3/R4 cell fate defects. (Gaengel and Mlodzik, 2003) The addition of argos^rlt/+ enhances the eye cell polarity phenotype seen in fz¹⁹/fz²⁰ animals. (Strutt and Strutt, 2003) About 12% of ommatidia in homozygous argos^rlt animals exhibit extra R cells. Of those that ommatidia that remain about 49% exhibit misrotations, about 6% exhibit achirality, and about 1% exhibit the wrong chirality. About 11% of ommatidia in argos^Δ7/argos^rlt animals exhibit extra R cells. Of those that ommatidia that remain about 52% exhibit misrotations, about 1% exhibit achirality, and about 1% exhibit the wrong chirality. About 16% of ommatidia in argos^5F4/argos^rlt animals exhibit extra R cells. Of those that ommatidia that remain about 53% exhibit misrotations, about 2% exhibit achirality, and about 0.3% exhibit the wrong chirality. (Strutt and Strutt, 2003) Photoreceptor orientation is abnormal. The majority of ommatidia have the normal number of photoreceptors, in normal trapezoidal configuration, though 17% of ommatidia have an extra photoreceptor cell. But 47% of photoreceptor clusters actually rotate more than 90^o. nmo mutants are epistatic to argos^rlt with respect to ommatidial precluster orientation. (Choi and Benzer, 1994)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement Reference aos^rlt has cell polarity defective phenotype, suppressible \| partially by Egfr^t1 (Gaengel and Mlodzik, 2003) aos^rlt has cell polarity defective phenotype, suppressible \| partially by nmo^P1 (Gaengel and Mlodzik, 2003) aos^rlt has cell polarity defective phenotype, suppressible \| partially by pnt^Δ88 (Gaengel and Mlodzik, 2003) aos^rlt has cell polarity defective phenotype, suppressible \| partially by Ras85D^e2F (Gaengel and Mlodzik, 2003)
Enhancer of
Statement Reference aos[+]/aos^rlt is an enhancer of planar polarity defective phenotype of Mtl^Scer\UAS.cMa, Scer\GAL4^hs.2sev (Muñoz-Descalzo et al., 2007) aos^rlt is an enhancer of cell polarity defective phenotype of fz²⁰/fz¹⁹ (Strutt and Strutt, 2003)
NOT Enhancer of
Statement Reference argos[+]/aos^rlt is a non-enhancer of visible phenotype of Scer\GAL4^en-e16E, kermit^GS2053 (Djiane and Mlodzik, 2010)
Suppressor of
Statement Reference aos^rlt is a suppressor of cell polarity defective phenotype of Ras64B^V14.Act5C (Strutt and Strutt, 2003)
NOT Suppressor of
Statement Reference argos[+]/aos^rlt is a non-suppressor of visible phenotype of Scer\GAL4^en-e16E, kermit^GS2053 (Djiane and Mlodzik, 2010)
Phenotype Manifest In
Suppressed by
Statement Reference aos^rlt has ommatidium phenotype, suppressible \| partially by Egfr^t1 (Gaengel and Mlodzik, 2003) aos^rlt has ommatidium phenotype, suppressible \| partially by nmo^P1 (Gaengel and Mlodzik, 2003) aos^rlt has ommatidium phenotype, suppressible \| partially by pnt^Δ88 (Gaengel and Mlodzik, 2003) aos^rlt has ommatidium phenotype, suppressible \| partially by Ras85D^e2F (Gaengel and Mlodzik, 2003)
Enhancer of
Statement Reference aos[+]/aos^rlt is an enhancer of ommatidium phenotype of Mtl^Scer\UAS.cMa, Scer\GAL4^hs.2sev (Muñoz-Descalzo et al., 2007) aos^rlt is an enhancer of ommatidium phenotype of fz²⁰/fz¹⁹ (Strutt and Strutt, 2003)
NOT Enhancer of
Statement Reference argos[+]/aos^rlt is a non-enhancer of wing hair phenotype of Scer\GAL4^en-e16E, kermit^GS2053 (Djiane and Mlodzik, 2010)
Suppressor of
Statement Reference aos^rlt is a suppressor of ommatidium phenotype of Rac1^V12.hs.sev (Fanto et al., 2000) aos^rlt is a suppressor of ommatidium phenotype of Ras64B^V14.Act5C (Strutt and Strutt, 2003) argos[+]/aos^rlt is a suppressor \| partially of eye phenotype of H^Scer\UAS.cMa/H^Scer\UAS.cMa, Scer\GAL4^GMR.PU (Protzer et al., 2008)
NOT Suppressor of
Statement Reference aos^rlt is a non-suppressor of ommatidium phenotype of ec^spok (Montrasio et al., 2007) argos[+]/aos^rlt is a non-suppressor of ommatidium phenotype of Scer\GAL4^hs.2sev, nmo^Scer\UAS.cUa (Fiehler and Wolff, 2008) argos[+]/aos^rlt is a non-suppressor of wing hair phenotype of Scer\GAL4^en-e16E, kermit^GS2053 (Djiane and Mlodzik, 2010)
Additional Comments
Genetic Interactions
Statement Reference The small-eye phenotype observed in animals expressing H[Scer\UAS.cMa] in the eye under the control of Scer\GAL4[GMR.PU] is partially suppressed in a argos[rlt]/+ mutant background. (Protzer et al., 2008) The ommatidial rotation defects observed in ec[spok] hemizygous adult eyes are not suppressed by argos[rlt]/+. (Montrasio et al., 2007) S^48-5/argos^rlt mutants show 3.74%+-1.99 misrotated ommatidia compared to 9.55%+-1.43 seen in S^48-5 mutants alone. (Gaengel and Mlodzik, 2003) Heterozygotes suppress the ommatidial polarity defects seen in Ras64B^V14.Act5C. (Strutt and Strutt, 2003)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescued by	aos^rlt is rescued by aos^sev.PF (Gaengel and Mlodzik, 2003)
Comments
Stocks ( 1 )
Bloomington	7336 aos^rlt
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 5 )
Reported As
Symbol Synonym	aos^rlt1 (Montrasio et al., 2007) aos^rlt (Fetting et al., 2009, Muñoz-Descalzo et al., 2007) argos^rlt (Harris et al., 2011, Protzer et al., 2008, Fiehler and Wolff, 2008, Djiane and Mlodzik, 2010) rlt¹ (Gaengel and Mlodzik, 2003, Brown and Freeman, 2003, Fanto, 2000, Fanto et al., 2000, ) unnamed (Choi and Benzer, 1994)
Name Synonym
Secondary FlyBase IDs

References ( 15 )
Research paper	Harris et al., 2011, J. Cell Biol. 194(1): 77--87 Cargo sorting to lysosome-related organelles regulates siRNA-mediated gene silencing. [FBrf0214287] Djiane and Mlodzik, 2010, PLoS ONE 5(6): e11228 The Drosophila GIPC homologue can modulate myosin based processes and planar cell polarity but is not essential for development. [FBrf0211141] Fetting et al., 2009, Development 136(19): 3323--3333 The cell adhesion molecules Echinoid and Friend of Echinoid coordinate cell adhesion and cell signaling to regulate the fidelity of ommatidial rotation in the Drosophila eye. [FBrf0208836] Fiehler and Wolff, 2008, Dev. Biol. 313(2): 533--544 Nemo is required in a subset of photoreceptors to regulate the speed of ommatidial rotation. [FBrf0203152] Protzer et al., 2008, J. Cell Sci. 121(19): 3167--3176 Hairless induces cell death by downregulation of EGFR signalling activity. [FBrf0207053] Montrasio et al., 2007, Dev. Dyn. 236(10): 2936--2942 A new allele uncovers the role of echinus in the control of ommatidial rotation in the Drosophila eye. [FBrf0202297] Muñoz-Descalzo et al., 2007, Int. J. Dev. Biol. 51(5): 379--387 Analysis of the role of the Rac/Cdc42 GTPases during planar cell polarity generation in Drosophila. [FBrf0200938] Brown and Freeman, 2003, Development 130(22): 5401--5412 Egfr signalling defines a protective function for ommatidial orientation in the Drosophila eye. [FBrf0167498] Gaengel and Mlodzik, 2003, Development 130(22): 5413--5423 Egfr signaling regulates ommatidial rotation and cell motility in the Drosophila eye via MAPK/Pnt signaling and the Ras effector Canoe/AF6. [FBrf0167499] Strutt and Strutt, 2003, Curr. Biol. 13(16): 1451--1457 EGF signaling and ommatidial rotation in the Drosophila eye. [FBrf0162001] Fanto et al., 2000, Curr. Biol. 10(16): 979--988 Nuclear signaling by Rac and Rho GTPases is required in the establishment of epithelial planar polarity in the Drosophila eye. [FBrf0129802] Choi and Benzer, 1994, Cell 78(1): 125--136 Rotation of photoreceptor clusters in the developing Drosophila eye requires the nemo gene. [FBrf0072827]
Supplementary material	Strutt and Strutt, 2003, Curr. Biol. 13(16): EGF signaling and ommatidial rotation in the Drosophila eye. [FBrf0173057] Fanto, 2000, Curr. Biol. 10(16): Nuclear signaling by Rac and Rho GTPases is required in the establishment of epithelial planar polarity in the Drosophila eye. [FBrf0131040]
Review	Wolff, 2003, Curr. Biol. 13(20): R813--R814 EGF receptor signaling: putting a new spin on eye development. [FBrf0173121]

Allele Dmel\aosrlt

Allele Dmel\aos^rlt