|Name||Saccharomyces cerevisiae UAS construct a of Castelli-Gair||FlyBase ID||FBal0038086|
|Feature type||allele||Associated gene||Dmel\Abd-B|
|Mutagen||in vitro construct - regulatory fusion|
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Controlled Vocabulary Terms
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|Nature of the Allele|
|Mutations Mapped to the Genome|
|Associated Sequence Data|
|Nature of the lesion|
|Carried in construct|
(Azpiazu and Morata, 1998, Li et al., 1999, Foronda et al., 2006, Castelli-Gair, 1998, Hirth et al., 2001, Meyer et al., 2002, Lovato et al., 2002, Lo et al., 2002, Castelli-Gair et al., 1994, Estrada and Sanchez-Herrero, 2001, Adachi-Yamada et al., 2005, Sprecher et al., 2004, DeFalco et al., 2004, Miguel-Aliaga and Thor, 2004, Greig and Akam, 1995, Casares et al., 1996, de Navas et al., 2006, Hueber et al., 2007, Zhai et al., 2012, Zhai et al., 2011, Chopra et al., 2011, Percival-Smith et al., 2005, Lovegrove et al., 2006)
|Phenotype Manifest In|
Expression of Abd-BScer\UAS.cCa under the control of Scer\GAL469B in the trunk segments results in the formation of ectopic spiracles.
When Abd-BScer\UAS.cCa is driven by Scer\GAL4sd-SG29.1 abnormal wing vein patterns and incisions in the wing margin are seen.
Expression of Abd-B[Scer\UAS.cCa] under the control of Scer\GAL4[dpp.blk1] results in transformation of the arista to tarsus.
Embryos expressing Abd-BScer\UAS.cCa under the control of Scer\GAL4twi.PB form ectopic clusters of male-specific somatic gonadal precursor (msSGP) cells in more anterior regions of the embryo than normal. Gonads do not form in these embryos and germ cells are either scattered or associated with msSGPs.
79% of anterior dMP2 neurons survive in late stage embryos expressing Abd-BScer\UAS.cCa under the control of Scer\GAL4Vap.P0201 (in contrast to wild-type embryos, where these neurons are lost by the late embryonic stage). 100% of anterior dMP2 neurons survive in late stage embryos expressing Abd-BScer\UAS.cCa under the control of Scer\GAL4elav-C155. Expression of Abd-BScer\UAS.cCa under the control of Scer\GAL4elav-C155 in late stage Abd-BM2/Abd-BM5 embryos results in the suppression of cell death normally seen in anterior dMP2 and MP1 neurons in wild-type late stage embryos.
Expression of Abd-BScer\UAS.cCa under the control of Scer\GAL4sca-537.4 results in a mutant phenotype in the embryonic tritocerebrum. The phenotype has a penetrance of more than 95%.
Expression of Abd-BScer\UAS.cCa in the mesoderm under the control of Scer\GAL4unspecified results in severe defects in muscle development; the skeletal muscle pattern is massively deranged and there appear to be no dorsal vessel cells (based upon staining for Mhc expression).
Expression of Abd-BScer\UAS.cCa under the control of Scer\GAL4lab.PH does not result in morphological defects in the tritocerebrum or any other part of the embryonic brain.
Expression of Abd-BScer\UAS.cCa (driven by Scer\GAL4dpp.blk1) results in inhibition of eye development by inducing apoptosis.
Scer\GAL469B-mediated expression in wild type causes a pair of posterior spiracles to develop in all trunk segments.
Scer\GAL4E132 induced expression in the eye-antennal disc causes transformation of the antenna into leg. Scer\GAL4Bx-MS1096 or Scer\GAL4C-765 induced expression in the wing disc causes transformation of the wing into haltere-like tissue. Scer\GAL4C-765 induced expression also causes malformed notum, fused scutellum and disorganised pattern of bristles. Scer\GAL4c739 and Scer\GAL4C-765 induced expression in the abdomen transforms A1 into a posterior abdominal segment.
Scer\GAL4twi.PG induced expression blocks the formation of the gonad primordium.
When driven by Scer\GAL4Kr.PM, Scer\GAL4h-1J3 or Scer\GAL469B, in a wild type background, Abd-BScer\UAS.cCa generates ectopic primordia for the posterior spiracle. These go on to develop filzkorper and spiracular hairs. Keilin's organs are suppressed in the Abd-BScer\UAS.cCa, Scer\GAL4h-1J3 or Abd-BScer\UAS.cCa, Scer\GAL4Kr.PM combination.
Abd-BScer\UAS.cCa/Scer\GAL4Bx-MS1096 is a suppressor | partially of visible phenotype of Ubxabx-1, Ubxbx-3, Ubxpbx-1/Ubxlac1
Abd-BScer\UAS.cCa/Scer\GAL4Bx-MS1096 is a suppressor | partially of visible phenotype of Ubxabx-1, Ubxlac1, Ubxpbx-1/Ubxbx-3
|Phenotype Manifest In|
Abd-BScer\UAS.cCa, Scer\GAL469B has embryonic/larval posterior spiracle | ectopic phenotype, suppressible by linG1
Abd-BScer\UAS.cCa, Scer\GAL4dpp.blk1 has arista phenotype, suppressible by pbScer\UAS.T:Avic\GFP-EGFP, Scer\GAL4dpp.blk1
Abd-BScer\UAS.cCa, Scer\GAL4dpp.blk1 has pretarsus | ectopic phenotype, suppressible by pbScer\UAS.T:Avic\GFP-EGFP, Scer\GAL4dpp.blk1
Abd-BScer\UAS.cCa/Scer\GAL4Bx-MS1096 is a suppressor | partially of metathoracic segment | ectopic phenotype of Ubxabx-1, Ubxbx-3, Ubxpbx-1/Ubxlac1
Abd-BScer\UAS.cCa/Scer\GAL4Bx-MS1096 is a suppressor | partially of metathoracic segment | ectopic phenotype of Ubxabx-1, Ubxlac1, Ubxpbx-1/Ubxbx-3
|NOT Suppressor of|
Co-expression of pb[Scer\UAS.T:Avic\GFP-EGFP] suppresses the arista-to-tarsus transformation seen in flies expressing Abd-B[Scer\UAS.cCa] under the control of Scer\GAL4[dpp.blk1].
Embryos coexpressing abd-AScer\UAS.cGa and Abd-BScer\UAS.cCa under the control of Scer\GAL4twi.PB have ectopic male-specific somatic gonadal precursor (msSGP) cells and ectopic somatic gonadal precursor (SGP) cells. A large group of SGPs and msSGPs associates with most of the germ cells in the region where the gonad would normally be coalescing and ectopic SGPs and msSGPs also associate with one another in more anterior regions.
Expression of Abd-BScer\UAS.cCa under the control of Scer\GAL4lab.PH does not rescue the tritocerebral defects seen in lab14 embryonic brains.
Scer\GAL469B-mediated expression of Abd-BScer\UAS.cCa in linG1 mutants does not cause ectopic posterior spiracles development in the trunk segments.
Ubxabx-1 Ubxbx-3 Ubxpbx-1/Ubxlac1 combination transforms the metathorax into mesothorax (including transformation of halteres into wings), Scer\GAL4Bx-MS1096 induced expression allows partial rescue.
|Complementation & Rescue Data|
|Stocks ( 2 )|
|Notes on Origin|
|External Crossreferences & Linkouts|
|Synonyms & Secondary IDs ( 3 )|
Saccharomyces cerevisiae UAS construct a of Castelli-Gair
|Secondary FlyBase IDs|
|References ( 25 )|
|Generate a list of|
|List References by type|
|Recent research papers ( 3 )|