Allele Dmel\Abd-B^Scer\UAS.cCa

General Information
Symbol	Dmel\Abd-B^Scer\UAS.cCa	Species	D. melanogaster
Name	Saccharomyces cerevisiae UAS construct a of Castelli-Gair	FlyBase ID	FBal0038086
Feature type	allele	Associated gene	Dmel\Abd-B

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02	Controlled Vocabulary Terms arista visible pretarsus
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Castelli-Gair et al., 1994)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: The cDNA coding region for Abd-B m variant cloned downstream of the Scer\GAL4 response element UAS. (Castelli-Gair et al., 1994)
Carried in construct	P{UAS-Abd-B.m.C} (Azpiazu and Morata, 1998, Li et al., 1999, Foronda et al., 2006, Castelli-Gair, 1998, Hirth et al., 2001, Meyer et al., 2002, Lovato et al., 2002, Lo et al., 2002, Castelli-Gair et al., 1994, Estrada and Sanchez-Herrero, 2001, Adachi-Yamada et al., 2005, Sprecher et al., 2004, DeFalco et al., 2004, Miguel-Aliaga and Thor, 2004, Greig and Akam, 1995, Casares et al., 1996, de Navas et al., 2006, Hueber et al., 2007, Zhai et al., 2012, Zhai et al., 2011, Chopra et al., 2011, Percival-Smith et al., 2005, Lovegrove et al., 2006)
Cytology
Phenotypic Data
Phenotypic Class
	decreased cell death, with Scer\GAL4^elav-C155 (Miguel-Aliaga and Thor, 2004) decreased cell death, with Scer\GAL4^Vap.P0201 (Miguel-Aliaga and Thor, 2004) lethal \| larval stage, with Scer\GAL4^pnr-MD237 (Casares et al., 1996) visible, with Scer\GAL4^dpp.blk1 (Percival-Smith et al., 2005)
Phenotype Manifest In
	abdominal segment 1, with Scer\GAL4^c739 (Casares et al., 1996) abdominal segment 1, with Scer\GAL4^C-765 (Casares et al., 1996) antenna, with Scer\GAL4^upd-E132 (Casares et al., 1996) arista, with Scer\GAL4^dpp.blk1 (Percival-Smith et al., 2005) dMP2 neuron \| embryonic stage 17, with Abd-B^M5, Scer\GAL4^elav-C155 (Miguel-Aliaga and Thor, 2004) dMP2 neuron \| embryonic stage 17, with Abd-B^M5/Abd-B^M2, Scer\GAL4^elav-C155 (Miguel-Aliaga and Thor, 2004) dMP2 neuron \| embryonic stage 17, with Scer\GAL4^elav-C155 (Miguel-Aliaga and Thor, 2004) dMP2 neuron \| embryonic stage 17, with Scer\GAL4^Vap.P0201 (Miguel-Aliaga and Thor, 2004) embryonic/larval posterior spiracle, with Scer\GAL4^69B (Castelli-Gair et al., 1994) embryonic/larval posterior spiracle, with Scer\GAL4^h-1J3 (Castelli-Gair et al., 1994) embryonic/larval posterior spiracle, with Scer\GAL4^Kr.PM (Castelli-Gair et al., 1994) embryonic/larval spiracle \| ectopic, with Scer\GAL4^69B (Lovegrove et al., 2006) embryonic dorsal vessel, with Scer\GAL4^unspecified (Lovato et al., 2002) embryonic somatic muscle, with Scer\GAL4^unspecified (Lovato et al., 2002) embryonic tritocerebrum, with Scer\GAL4^sca-537.4 (Sprecher et al., 2004) eye, with Scer\GAL4^dpp.blk1 (Plaza et al., 2001) fat body/gonad primordium, with Scer\GAL4^twi.PG (Greig and Akam, 1995) filzkorper \| ectopic, with Scer\GAL4^69B (Castelli-Gair et al., 1994) filzkorper \| ectopic, with Scer\GAL4^h-1J3 (Castelli-Gair et al., 1994) filzkorper \| ectopic, with Scer\GAL4^Kr.PM (Castelli-Gair et al., 1994) gonad \| embryonic stage, with Scer\GAL4^twi.PB (DeFalco et al., 2004) gonadal sheath proper primordium, with Scer\GAL4^twi.PB (DeFalco et al., 2004) MP1 neuron \| embryonic stage 17, with Abd-B^M5, Scer\GAL4^elav-C155 (Miguel-Aliaga and Thor, 2004) MP1 neuron \| embryonic stage 17, with Abd-B^M5/Abd-B^M2, Scer\GAL4^elav-C155 (Miguel-Aliaga and Thor, 2004) pretarsus \| ectopic, with Scer\GAL4^dpp.blk1 (Percival-Smith et al., 2005) scutellum, with Scer\GAL4^C-765 (Casares et al., 1996) scutum, with Scer\GAL4^C-765 (Casares et al., 1996) scutum & macrochaeta, with Scer\GAL4^C-765 (Casares et al., 1996) spiracular hair \| ectopic, with Scer\GAL4^69B (Castelli-Gair et al., 1994) spiracular hair \| ectopic, with Scer\GAL4^h-1J3 (Castelli-Gair et al., 1994) spiracular hair \| ectopic, with Scer\GAL4^Kr.PM (Castelli-Gair et al., 1994) wing, with Scer\GAL4^Bx-MS1096 (Casares et al., 1996) wing, with Scer\GAL4^C-765 (Casares et al., 1996) wing margin, with Scer\GAL4^sd-SG29.1 (Adachi-Yamada et al., 2005) wing vein, with Scer\GAL4^sd-SG29.1 (Adachi-Yamada et al., 2005)
Detailed Description
	Statement Reference Expression of Abd-B^Scer\UAS.cCa under the control of Scer\GAL4^69B in the trunk segments results in the formation of ectopic spiracles. (Lovegrove et al., 2006) When Abd-B^Scer\UAS.cCa is driven by Scer\GAL4^sd-SG29.1 abnormal wing vein patterns and incisions in the wing margin are seen. (Adachi-Yamada et al., 2005) Expression of Abd-B[Scer\UAS.cCa] under the control of Scer\GAL4[dpp.blk1] results in transformation of the arista to tarsus. (Percival-Smith et al., 2005) Embryos expressing Abd-B^Scer\UAS.cCa under the control of Scer\GAL4^twi.PB form ectopic clusters of male-specific somatic gonadal precursor (msSGP) cells in more anterior regions of the embryo than normal. Gonads do not form in these embryos and germ cells are either scattered or associated with msSGPs. (DeFalco et al., 2004) 79% of anterior dMP2 neurons survive in late stage embryos expressing Abd-B^Scer\UAS.cCa under the control of Scer\GAL4^Vap.P0201 (in contrast to wild-type embryos, where these neurons are lost by the late embryonic stage). 100% of anterior dMP2 neurons survive in late stage embryos expressing Abd-B^Scer\UAS.cCa under the control of Scer\GAL4^elav-C155. Expression of Abd-B^Scer\UAS.cCa under the control of Scer\GAL4^elav-C155 in late stage Abd-B^M2/Abd-B^M5 embryos results in the suppression of cell death normally seen in anterior dMP2 and MP1 neurons in wild-type late stage embryos. (Miguel-Aliaga and Thor, 2004) Expression of Abd-B^Scer\UAS.cCa under the control of Scer\GAL4^sca-537.4 results in a mutant phenotype in the embryonic tritocerebrum. The phenotype has a penetrance of more than 95%. (Sprecher et al., 2004) Expression of Abd-B^Scer\UAS.cCa in the mesoderm under the control of Scer\GAL4^unspecified results in severe defects in muscle development; the skeletal muscle pattern is massively deranged and there appear to be no dorsal vessel cells (based upon staining for Mhc expression). (Lovato et al., 2002) Expression of Abd-B^Scer\UAS.cCa under the control of Scer\GAL4^lab.PH does not result in morphological defects in the tritocerebrum or any other part of the embryonic brain. (Hirth et al., 2001) Expression of Abd-B^Scer\UAS.cCa (driven by Scer\GAL4^dpp.blk1) results in inhibition of eye development by inducing apoptosis. (Plaza et al., 2001) Scer\GAL4^69B-mediated expression in wild type causes a pair of posterior spiracles to develop in all trunk segments. (Castelli-Gair, 1998) Scer\GAL4^E132 induced expression in the eye-antennal disc causes transformation of the antenna into leg. Scer\GAL4^Bx-MS1096 or Scer\GAL4^C-765 induced expression in the wing disc causes transformation of the wing into haltere-like tissue. Scer\GAL4^C-765 induced expression also causes malformed notum, fused scutellum and disorganised pattern of bristles. Scer\GAL4^c739 and Scer\GAL4^C-765 induced expression in the abdomen transforms A1 into a posterior abdominal segment. (Casares et al., 1996) Scer\GAL4^twi.PG induced expression blocks the formation of the gonad primordium. (Greig and Akam, 1995) When driven by Scer\GAL4^Kr.PM, Scer\GAL4^h-1J3 or Scer\GAL4^69B, in a wild type background, Abd-B^Scer\UAS.cCa generates ectopic primordia for the posterior spiracle. These go on to develop filzkorper and spiracular hairs. Keilin's organs are suppressed in the Abd-B^Scer\UAS.cCa, Scer\GAL4^h-1J3 or Abd-B^Scer\UAS.cCa, Scer\GAL4^Kr.PM combination. (Castelli-Gair et al., 1994)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement Reference Abd-B^Scer\UAS.cCa, Scer\GAL4^dpp.blk1 has visible phenotype, suppressible by pb^{Scer\UAS.T:Avic\GFP-EGFP}, Scer\GAL4^dpp.blk1 (Percival-Smith et al., 2005)
Suppressor of
Statement Reference Abd-B^Scer\UAS.cCa/Scer\GAL4^Bx-MS1096 is a suppressor \| partially of visible phenotype of Ubx^abx-1, Ubx^bx-3, Ubx^pbx-1/Ubx^lac1 (Casares et al., 1996, Casares et al., 1996) Abd-B^Scer\UAS.cCa/Scer\GAL4^Bx-MS1096 is a suppressor \| partially of visible phenotype of Ubx^abx-1, Ubx^lac1, Ubx^pbx-1/Ubx^bx-3 (Casares et al., 1996) Abd-B^Scer\UAS.cCa/Scer\GAL4^Bx-MS1096 is a suppressor \| partially of visible phenotype of Ubx^bx-3, Ubx^lac1, Ubx^abx-1/Ubx^pbx-1 (Casares et al., 1996)
Phenotype Manifest In
Suppressed by
Statement Reference Abd-B^Scer\UAS.cCa, Scer\GAL4^69B has embryonic/larval posterior spiracle \| ectopic phenotype, suppressible by lin^G1 (Castelli-Gair, 1998) Abd-B^Scer\UAS.cCa, Scer\GAL4^dpp.blk1 has arista phenotype, suppressible by pb^{Scer\UAS.T:Avic\GFP-EGFP}, Scer\GAL4^dpp.blk1 (Percival-Smith et al., 2005) Abd-B^Scer\UAS.cCa, Scer\GAL4^dpp.blk1 has pretarsus \| ectopic phenotype, suppressible by pb^{Scer\UAS.T:Avic\GFP-EGFP}, Scer\GAL4^dpp.blk1 (Percival-Smith et al., 2005) Abd-B^Scer\UAS.cCa, Scer\GAL4^twi.PG has fat body/gonad primordium phenotype, suppressible by abd-A^Scer\UAS.cGa (Greig and Akam, 1995)
Suppressor of
Statement Reference Abd-B^Scer\UAS.cCa/Scer\GAL4^Bx-MS1096 is a suppressor \| partially of metathoracic segment \| ectopic phenotype of Ubx^abx-1, Ubx^bx-3, Ubx^pbx-1/Ubx^lac1 (Casares et al., 1996, Casares et al., 1996) Abd-B^Scer\UAS.cCa/Scer\GAL4^Bx-MS1096 is a suppressor \| partially of metathoracic segment \| ectopic phenotype of Ubx^abx-1, Ubx^lac1, Ubx^pbx-1/Ubx^bx-3 (Casares et al., 1996) Abd-B^Scer\UAS.cCa/Scer\GAL4^Bx-MS1096 is a suppressor \| partially of metathoracic segment \| ectopic phenotype of Ubx^bx-3, Ubx^lac1, Ubx^abx-1/Ubx^pbx-1 (Casares et al., 1996)
NOT Suppressor of
Statement Reference Abd-B^Scer\UAS.cCa/Scer\GAL4^lab.PH is a non-suppressor of embryonic tritocerebrum phenotype of lab¹⁴ (Hirth et al., 2001)
Other
Statement Reference Abd-B^Scer\UAS.cCa, Scer\GAL4^twi.PB, abd-A^Scer\UAS.cGa has gonadal sheath proper primordium phenotype (DeFalco et al., 2004, DeFalco et al., 2004)
Additional Comments
Genetic Interactions
Statement Reference Co-expression of pb[Scer\UAS.T:Avic\GFP-EGFP] suppresses the arista-to-tarsus transformation seen in flies expressing Abd-B[Scer\UAS.cCa] under the control of Scer\GAL4[dpp.blk1]. (Percival-Smith et al., 2005) Embryos coexpressing abd-A^Scer\UAS.cGa and Abd-B^Scer\UAS.cCa under the control of Scer\GAL4^twi.PB have ectopic male-specific somatic gonadal precursor (msSGP) cells and ectopic somatic gonadal precursor (SGP) cells. A large group of SGPs and msSGPs associates with most of the germ cells in the region where the gonad would normally be coalescing and ectopic SGPs and msSGPs also associate with one another in more anterior regions. (DeFalco et al., 2004) Expression of Abd-B^Scer\UAS.cCa under the control of Scer\GAL4^lab.PH does not rescue the tritocerebral defects seen in lab¹⁴ embryonic brains. (Hirth et al., 2001) Scer\GAL4^69B-mediated expression of Abd-B^Scer\UAS.cCa in lin^G1 mutants does not cause ectopic posterior spiracles development in the trunk segments. (Castelli-Gair, 1998) Ubx^abx-1 Ubx^bx-3 Ubx^pbx-1/Ubx^lac1 combination transforms the metathorax into mesothorax (including transformation of halteres into wings), Scer\GAL4^Bx-MS1096 induced expression allows partial rescue. (Casares et al., 1996) Scer\GAL4^twi.PG induced expression of Abd-B^Scer\UAS.cCa blocks formation of the gonadal primordium, coexpression of Abd-B^Scer\UAS.cCa and abd-A^Scer\UAS.cGa restores gonadal mesoderm. (Greig and Akam, 1995)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescues	Abd-B^Scer\UAS.cCa/Scer\GAL4^Abd-B-LDN rescues Abd-B^M1/Abd-B^LDN (de Navas et al., 2006) Abd-B^Scer\UAS.cCa/Scer\GAL4^Vap.P0201 rescues Abd-B^M5/Abd-B^M2 (Miguel-Aliaga and Thor, 2004)
Comments	The loss of posterior dMP2 neurons that is seen in late stage Abd-B^M2/Abd-B^M5 embryos is rescued by expression of Abd-B^Scer\UAS.cCa under the control of Scer\GAL4^Vap.P0201. (Miguel-Aliaga and Thor, 2004)
Stocks ( 2 )
Bloomington	913 w¹; P{UAS-Abd-B.m.C}1.1
Kyoto	106120 w¹; P{UAS-Abd-B.m.C}1.1
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	Abd-B^Scer\UAS.cCa Abd-B^UAS.cCa
Name Synonym	Saccharomyces cerevisiae UAS construct a of Castelli-Gair (Castelli-Gair et al., 1994)
Secondary FlyBase IDs

References ( 25 )

Generate a list of	All references relating to this allele ( 25 )

List References by type	Research paper ( 24 ) Supplementary material ( 1 )
Recent research papers ( 3 )
	Zhai et al., 2012, PLoS Genet. 8(3): e1002582 Antagonistic regulation of apoptosis and differentiation by the cut transcription factor represents a tumor-suppressing mechanism in Drosophila. [FBrf0217859] Chopra et al., 2011, Mol. Cell 42(6): 837--844 The Polycomb Group Mutant esc Leads to Augmented Levels of Paused Pol II in the Drosophila Embryo. [FBrf0214009] Zhai et al., 2011, Biochem. Biophys. Res. Commun. 414(4): 761--766 Functional dissection of the Hox protein Abdominal-B in Drosophila cell culture. [FBrf0216579] Show all research papers ...

Allele Dmel\Abd-BScer\UAS.cCa

Allele Dmel\Abd-B^Scer\UAS.cCa