Allele Dmel\Adh^Wa-F.tCa

General Information
Symbol	Dmel\Adh^Wa-F.tCa	Species	D. melanogaster
Name		FlyBase ID	FBal0038129
Feature type	allele	Associated gene	Dmel\Adh

Allele class
Mutagen	in vitro construct - genomic fragment, in vitro construct - site directed mutagenesis
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - genomic fragment (Laurie-Ahlberg and Stam, 1987, Choudhary and Laurie, 1991, Laurie and Stam, 1994, Stam and Laurie, 1996, Parsch et al., 1997) in vitro construct - site directed mutagenesis (Parsch et al., 1997)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype	Adh^Wa-F (Laurie-Ahlberg and Stam, 1987, Choudhary and Laurie, 1991, Laurie and Stam, 1994)
Nature of the lesion	Statement Reference 8.6kb SacI-ClaI fragment of Adh^Wa-F. (Parsch et al., 1997) 8.0kb SacI-ClaI fragment containing 5.5kb upstream sequences, coding region for the Adh^Wa-F mutation and 2.7kb downstream sequences. (Stam and Laurie, 1996) Construct: 8.0kb SacI ClaI fragment carrying the Adh^Wa-F mutation. (Laurie and Stam, 1994) 8.6kb SacI ClaI fragment carrying the Adh^Wa-F mutation. Fragment includes about 0.8kb of 3' flanking DNA and 5.4kb of 5' flanking DNA. (Laurie-Ahlberg and Stam, 1987)
Carried in construct	P{Adh.Wa-F} (Carlini and Stephan, 2003, Pal-Bhadra et al., 2002, Laurie and Stam, 1994, Parsch et al., 2000, Pal-Bhadra et al., 1999, Parsch et al., 1999, Bhadra et al., 1999, Pal-Bhadra et al., 1997, Choudhary and Laurie, 1991, Laurie-Ahlberg and Stam, 1987, Parsch et al., 1997, Stam and Laurie, 1996, Carlini, 2004)
Cytology
Phenotypic Data
Phenotypic Class

Phenotype Manifest In

Detailed Description
	Statement Reference
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

Comments
	A transposon-borne wild type fragment derived from the Adh^Wa-F allele of Kreitman (FBrf0040028) is compared to the genomic form of the mutant allele. (Choudhary and Laurie, 1991)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	Adh^Wa-F.tCa Adh^Wa-F.tPa
Name Synonym
Secondary FlyBase IDs
	FBal0059450
References ( 13 )
Research paper	Carlini, 2004, J. Evol. Biol. 17(4): 779--785 Experimental reduction of codon bias in the Drosophila alcohol dehydrogenase gene results in decreased ethanol tolerance of adult flies. [FBrf0179153] Carlini and Stephan, 2003, Genetics 163(1): 239--243 In vivo introduction of unpreferred synonymous codons into the Drosophila Adh gene results in reduced levels of ADH protein. [FBrf0155816] Pal-Bhadra et al., 2002, Mol. Cell 9(2): 315--327 RNAi related mechanisms affect both transcriptional and posttranscriptional transgene silencing in Drosophila. [FBrf0145115] Parsch et al., 2000, Genetics 156(1): 219--227 Deletion of a conserved regulatory element in the Drosophila Adh gene leads to increased alcohol dehydrogenase activity but also delays development. [FBrf0130014] Bhadra et al., 1999, Genetics 152(1): 249--268 Role of the male specific lethal (msl) genes in modifying the effects of sex chromosomal dosage in Drosophila. [FBrf0108144] Pal-Bhadra et al., 1999, Cell 99(1): 35--46 Cosuppression of nonhomologous transgenes in Drosophila involves mutually related endogenous sequences. [FBrf0111995] Parsch et al., 1999, Genetics 151(2): 667--674 A highly conserved sequence in the 3'-untranslated region of the Drosophila Adh gene plays a functional role in ADH expression. [FBrf0107071] Pal-Bhadra et al., 1997, Cell 90(3): 479--490 Cosuppression in Drosophila: gene silencing of Alcohol dehydrogenase by white-Adh transgenes is Polycomb dependent. [FBrf0098324] Parsch et al., 1997, Proc. Natl. Acad. Sci. U.S.A. 94(3): 928--933 Site-directed mutations reveal long-range compensatory interactions in the Adh gene of Drosophila melanogaster. [FBrf0092651] Stam and Laurie, 1996, Genetics 144(4): 1559--1564 Molecular dissection of a major gene effect on a quantitative trait: the level of alcohol dehydrogenase expression in Drosophila melanogaster. [FBrf0091178] Laurie and Stam, 1994, Genetics 138(2): 379--385 The effect of an intronic polymorphism on alcohol dehydrogenase expression in Drosophila melanogaster. [FBrf0076546] Choudhary and Laurie, 1991, Genetics 129: 481--488 Use of in vitro mutagenesis to analyze the molecular basis of the difference in Adh expression associated with the allozyme polymorphism in Drosophila melanogaster. [FBrf0054161] Laurie-Ahlberg and Stam, 1987, Genetics 115: 129--140 Use of P-element-mediated transformation to identify the molecular basis of naturally occurring variants affecting Adh expression in Drosophila melanogaster. [FBrf0046345]

Allele Dmel\AdhWa-F.tCa

Allele Dmel\Adh^Wa-F.tCa