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General Information
Symbol
Dmel\BicDr8
Species
D. melanogaster
Name
FlyBase ID
FBal0038145
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
point mutation
Nucleotide change:

G17463368A

Amino acid change:

W416term | BicD-PA; W416term | BicD-PB; W416term | BicD-PC; W416term | BicD-PD

Reported amino acid change:

W416term

Comment:

This is in addition to the A40V substitution of the parental BicDr chromosome. Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Amino acid replacement: W416term.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

"Bic-D[mom]" females (BicDr8/Df(2L)TW119 females in which developmental lethality has been rescued using heat-shock induced expression of BicDhs.PS, and then BicDhs.PS expression has been stopped once oocyte fate is established by reducing the temperature) mostly lack the enrichment of yolk granules normally seen at the posterior of stage 8-9 oocytes.

Almost no eggs laid by heterozygous females display a fused dorsal appendage phenotype.

"Bic-D[mom]" (Bic-D mid-oogenesis mutant) females (BicDr8/Df(2L)TW119 females carrying BicDhs.PS in which regular heat shocks (two 37[o]C 30 minute heat shocks per day for 3-4 days starting in the third larval instar/early pupal stage) have been used to overcome the developmental lethality associated with BicDr8/Df(2L)TW119, and then the adults have been transferred to 18[o]C to stop BicDhs.PS expression) lay eggs with phenotypes ranging from partially fused or fused dorsal appendages to reduced or absent dorsal appendages. In many cases, the dorsal appendage remnants are located more posteriorly on the eggshell than normal. The operculum is often reduced or missing, and small eggs lacking the anterior chorion are also seen.

Fertile over BicD1, and viable but sterile over BicDr or BicDR26. Lethality is complete at 29oC, but some adults escape at 18oC. Survival can be markedly improved by raising the flies on apple juice-agar medium supplemented with live yeast. Resulting adults are uncoordinated and generally die within a couple of days. Ovaries contain only egg chambers with 16 polyploid nuclei and no oocyte. Germ line clones give rise to 16-nurse cell chambers with no oocyte. Reorganization of ring canals does not occur. The largest ring canal does not move to the posterior end of the cluster and is found in apparently random positions in the posterior half of the egg chamber.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Other
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

Eggs from most BicDr8 and grk3 transheterozygotes display a fused dorsal appendage phenotype.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (7)