Allele Dmel\RpL32^+t3.3

General Information
Symbol	Dmel\RpL32^+t3.3	Species	D. melanogaster
Name		FlyBase ID	FBal0038903
Feature type	allele	Created / Updated	2006-08-20/2006-08-20
Associated gene	Dmel\RpL32

Allele class
Mutagen	in vitro construct \| genomic fragment
Nature of the Allele
Allele class
Mutagen	in vitro construct \| genomic fragment (Kongsuwan et al., 1985, Schweisguth et al., 1990, Tamate et al., 1990, Crozatier et al., 1992)
Mapped Features and Mutations
Type Symbol & Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: A genomic 3.3kb fragment containing the gene for the large subunit ribosomal protein 49, plus about 0.6kb of downstream and about 2.1kb of upstream DNA. (Kongsuwan et al., 1985) Fragment containing the RpL32 gene. (Tamate et al., 1990)
Assay mode	In transgenic Drosophila (intraspecific) (Schweisguth et al., 1990, Tamate et al., 1990, Kongsuwan et al., 1985, Crozatier et al., 1992)
Carried in construct	P{RP49} (Tamate et al., 1990, Kongsuwan et al., 1985, Crozatier et al., 1992, Schweisguth et al., 1990)
Cytology
Phenotypic Data
Phenotypic Class
	wild-type (Crozatier et al., 1992)
Phenotype Manifest In

Detailed Description
	Statement Reference Specifically suppresses the dominant phenotype of RpL32 mutations, both with respect to bristle phenotype and developmental rate. (Kongsuwan et al., 1985)
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments	RpL32^+t3.3 rescues the Minute phenotype of Ts(YLt;3Lt)B81+Dp(3;1)67A. (Crozatier et al., 1992)
Stocks ( 2 )
Bloomington	2352 Df(3R)X3F, P{RP49}A3-84F e¹/TM3, Sb¹ 3546 Df(3R)B81, P{RP49}F2-80A e¹/TM3, Sb¹; Dp(3;1)67A
Notes on Origin
Discoverer

Comments
	Some inserts of this allele are less effective than others at suppressing the RpL32 phenotype. (Kongsuwan et al., 1985) RpL32 expression has been studied in flies carrying an extra copy of the RpL32 gene (RpL32^+t3.3). (Tamate et al., 1990)
Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	RpL32^+t3.3 unnamed (Cook, 2006.1.31)
Name Synonym
Secondary FlyBase IDs

References ( 5 )
Research paper	Crozatier et al., 1992, Genetics 131: 905--916 Single amino acid exchanges in separate domains of the Drosophila serendipity zinc finger protein cause embryonic and sex biased lethality. [FBrf0056268] Schweisguth et al., 1990, Genes Dev. 4: 922--931 The serendipity gene encodes a membrane-associated protein required for the cellularization of the Drosophila embryo. [FBrf0051866] Tamate et al., 1990, Molec. gen. Genet. 221: 171--175 Overproduction and translational regulation of Rp49 ribosomal protein mRNA in transgenic Drosophila carrying extra copies of the gene. [FBrf0052589] Kongsuwan et al., 1985, Nature 317(6037): 555--558 A Drosophila Minute gene encodes a ribosomal protein. [FBrf0043337]
Personal communication to FlyBase	Cook, 2006.1.31, Characterization of deletions and Minutes in chromosomal region 99-100. Characterization of deletions and Minutes in chromosomal region 99-100. [FBrf0191633]

Allele Dmel\RpL32+t3.3

Allele Dmel\RpL32^+t3.3