A Database of Drosophila Genes & Genomes

FB2008_06, released July 3, 2008
 

Allele Dmel\Rac1L89.Scer\UAS

General Information
SymbolDmel\Rac1L89.Scer\UASSpeciesD. melanogaster
NameFlyBase IDFBal0038992
Feature typealleleCreated / Updated2006-08-20/2006-08-20
Associated geneDmel\Rac1
Allele class
Mutagenin vitro construct | regulatory fusion
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Allele class
Mutagen
Mapped Features and Mutations
Type
Symbol & Location
Additional Notes
References
 
 
 
 
Associated Sequence Data
DDBJ /
EMBL /
GenBank
DNA sequence
Protein sequence
Name
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Progenitor genotype
      Nature of the lesion
      Statement
      Reference
      Construct: Amino acid replacement: S89L. A dominant-negative form of Rac1 is expressed under the control of Scer\UAS regulatory sequences.
      Assay mode
      Carried in construct
      Cytology
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      hide Phenotype Manifest In
      photoreceptor cell & axon, with Scer\GAL4GMR.PF
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      Statement
      Reference
      Only 15% embryos die when in combination with Scer\GAL4elav.PLu, surviving adults are completely viable. When in combination with Scer\GAL41407 one copy of Rac1L89.Scer\UAS causes some axonal loss between the lateral and dorsal clusters, two copies causes greater loss. One copy of Rac1Scer\UAS.cLa ameliorates this axonal loss. Scer\GAL4how-24B causes excessive fusion of the segmentally repeated myoblast cells.
      Scer\GAL4elav-C155-mediated expression causes early ISNb growth cone arrest, axons never reach distal muscle targets. Scer\GAL4elav-C155-mediated expression slightly disrupts CNS axon pathway, gaps between segments suggest failure in axon extension. Coexpression of Rac1Scer\UAS.cLa under the same Scer\GAL4 driver causes a massive failure in axon extension, dramatically enhancing the effect of Rac1L89.Scer\UAS. Axons that do extend follow abnormal trajectories.
      When expression is driven by Scer\GAL454 the eye is deformed.
      Expression of Rac1L89.Scer\UAS by Scer\GAL4prd.RG1 has no effect on cytokinesis or mitosis.
      Rac1L89.Scer\UAS driven by Scer\GAL4repo produces embryos that show ectopic lamellar like projections as well as failed inter-connection of the lateral line glia. The underlying sensory axonal tracts defasciculate. Misplacements od sensory appear to be associated with glia.
      Expression of Rac1L89.Scer\UAS in border cells, driven by Scer\GAL4slbo.2.6, inhibits the migration of border cell clusters to the oocyte. However, the apical cap of these egg chambers forms and is shed normally.
      Expression of Rac1[L89.Scer\UAS] under the control of Scer\GAL4[GMR.PF] results in severe defects in the R-cell projection pattern in the optic lobe.
      Expression of Rac1[L89.Scer\UAS] under the control of Scer\GAL4[ems.HRE] eliminates the spiracular chamber.
      hide Interactions
      hide Phenotypic Class
      hideEnhanced by
      Statement
      Reference
      hide Phenotype Manifest In
      hideEnhanced by
      Statement
      Reference
      Rac1L89.Scer\UAS, Scer\GAL4GMR.PF has photoreceptor cell & axon phenotype, enhanceable by bure10/bur[+]
      hideSuppressed by
      Statement
      Reference
      hide Additional Comments
      hide Genetic Interactions
      Statement
      Reference
      The R-cell axon projection defects caused by expression of Rac1[L89.Scer\UAS] under the control of Scer\GAL4[GMR.PF] are enhanced by bur[e10]/+.
      hide Xenogenetic Interactions
      Statement
      Reference
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      Comments
      hide Stocks ( 2 )
      Bloomington
      hide Notes on Origin
      Discoverer
      hide Synonyms & Secondary IDs ( 6 )
      Reported As
      Symbol Synonym
      Rac1L89.Scer\UAS
       
      Rac1L89.UAS
       
      Rac1L89
       
      Name Synonym
      Secondary FlyBase IDs
      • FBal0038991
      hide References ( 11 )
      Research paper
      Long et al., 2006, Genetics 172(3): 1633--1642
      De novo GMP synthesis is required for axon guidance in Drosophila. [FBrf0190740]
      Lovegrove et al., 2006, Curr. Biol. 16(22): 2206--2216
      Coordinated control of cell adhesion, polarity, and cytoskeleton underlies Hox-induced organogenesis in Drosophila. [FBrf0194228]
      Medioni and Noselli, 2005, Development 132(13): 3069--3077
      Dynamics of the basement membrane in invasive epithelial clusters in Drosophila. [FBrf0187473]
      Sepp and Auld, 2003, Development 130(9): 1825--1835
      RhoA and Rac1 GTPases mediate the dynamic rearrangement of actin in peripheral glia. [FBrf0158880]
      Magie et al., 2002, Development 129(16): 3771--3782
      Rho1 interacts with p120(ctn) and alpha-catenin, and regulates cadherin-based adherens junction components in Drosophila. [FBrf0151270]
      Lee and Luo, 1999, Neuron 22(3): 451--461
      Mosaic analysis with a repressible neurotechnique cell marker for studies of gene function in neuronal morphogenesis. [FBrf0107846]
      Prokopenko et al., 1999, Genes Dev. 13(17): 2301--2314
      A putative exchange factor for rho1 GTPase is required for initiation of cytokinesis in Drosophila. [FBrf0111464]
      Kaufmann et al., 1998, Development 125(3): 453--461
      Drosophila Rac1 controls motor axon guidance. [FBrf0100716]
      Luo et al., 1994, Genes Dev. 8(15): 1787--1802
      Distinct morphogenetic functions of similar small GTPases: Drosophila Drac1 is involved in axonal outgrowth and myoblast fusion. [FBrf0076490]
      Review
      Paululat et al., 1999, Mech. Dev. 83(1,2): 17--26
      Essential genes for myoblast fusion in Drosophila embryogenesis. [FBrf0108990]
      Abstract
      Paladi and Tepass, 2000, A. Dros. Res. Conf. 41: 436C
      Requirement of Rho GTPases in the development and migration of embryonic hemocytes in Drosophila. [FBrf0126564]