Allele Dmel\Rac1^L89.Scer\UAS

General Information
Symbol	Dmel\Rac1^L89.Scer\UAS	Species	D. melanogaster
Name		FlyBase ID	FBal0038992
Feature type	allele	Created / Updated	2006-08-20/2006-08-20
Associated gene	Dmel\Rac1

Allele class
Mutagen	in vitro construct \| regulatory fusion
Nature of the Allele
Allele class
Mutagen	in vitro construct \| regulatory fusion (Luo et al., 1994)
Mapped Features and Mutations
Type Symbol & Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Amino acid replacement: S89L. A dominant-negative form of Rac1 is expressed under the control of Scer\UAS regulatory sequences. (Luo et al., 1994)
Assay mode	In transgenic Drosophila (intraspecific) (Luo et al., 1994)
Carried in construct	P{UAS-Rac1.L89} (Medioni and Noselli, 2005, Kaufmann et al., 1998, Sepp and Auld, 2003, Prokopenko et al., 1999, Magie et al., 2002, Luo et al., 1994, Paululat et al., 1999, Lee and Luo, 1999, Long et al., 2006, Lovegrove et al., 2006)
Cytology
Phenotypic Data
Phenotypic Class
	lethal \| first instar larval stage, with Scer\GAL4¹⁴⁰⁷ (Luo et al., 1994) lethal \| first instar larval stage, with Scer\GAL4^how-24B (Luo et al., 1994) lethal \| first instar larval stage, with Scer\GAL4⁶⁰ (Luo et al., 1994) visible, with Scer\GAL4⁵⁴ (Lee and Luo, 1999) neuroanatomy defective, with Scer\GAL4^GMR.PF (Long et al., 2006)
Phenotype Manifest In
	border follicle cell, with Scer\GAL4^slbo.2.6 (Medioni and Noselli, 2005) polar follicle cell, with Scer\GAL4^slbo.2.6 (Medioni and Noselli, 2005) peripheral glial cell, with Scer\GAL4^repo (Sepp and Auld, 2003) abdominal anterior fascicle, with Scer\GAL4^elav-C155 (Kaufmann et al., 1998) axon, with Scer\GAL4^elav-C155 (Kaufmann et al., 1998) eye, with Scer\GAL4⁵⁴ (Lee and Luo, 1999) abdominal anterior fascicle, with Scer\GAL4¹⁴⁰⁷ (Luo et al., 1994) myoblast, with Scer\GAL4^how-24B (Luo et al., 1994) photoreceptor cell & axon, with Scer\GAL4^GMR.PF (Long et al., 2006) embryonic spiracle, with Scer\GAL4^ems.HRE (Lovegrove et al., 2006)
Detailed Description
	Statement Reference Only 15% embryos die when in combination with Scer\GAL4^elav.PLu, surviving adults are completely viable. When in combination with Scer\GAL4¹⁴⁰⁷ one copy of Rac1^L89.Scer\UAS causes some axonal loss between the lateral and dorsal clusters, two copies causes greater loss. One copy of Rac1^Scer\UAS.cLa ameliorates this axonal loss. Scer\GAL4^how-24B causes excessive fusion of the segmentally repeated myoblast cells. (Luo et al., 1994) Scer\GAL4^elav-C155-mediated expression causes early ISNb growth cone arrest, axons never reach distal muscle targets. Scer\GAL4^elav-C155-mediated expression slightly disrupts CNS axon pathway, gaps between segments suggest failure in axon extension. Coexpression of Rac1^Scer\UAS.cLa under the same Scer\GAL4 driver causes a massive failure in axon extension, dramatically enhancing the effect of Rac1^L89.Scer\UAS. Axons that do extend follow abnormal trajectories. (Kaufmann et al., 1998) When expression is driven by Scer\GAL4⁵⁴ the eye is deformed. (Lee and Luo, 1999) Expression of Rac1^L89.Scer\UAS by Scer\GAL4^prd.RG1 has no effect on cytokinesis or mitosis. (Prokopenko et al., 1999) Rac1^L89.Scer\UAS driven by Scer\GAL4^repo produces embryos that show ectopic lamellar like projections as well as failed inter-connection of the lateral line glia. The underlying sensory axonal tracts defasciculate. Misplacements od sensory appear to be associated with glia. (Sepp and Auld, 2003) Expression of Rac1^L89.Scer\UAS in border cells, driven by Scer\GAL4^slbo.2.6, inhibits the migration of border cell clusters to the oocyte. However, the apical cap of these egg chambers forms and is shed normally. (Medioni and Noselli, 2005) Expression of Rac1[L89.Scer\UAS] under the control of Scer\GAL4[GMR.PF] results in severe defects in the R-cell projection pattern in the optic lobe. (Long et al., 2006) Expression of Rac1[L89.Scer\UAS] under the control of Scer\GAL4[ems.HRE] eliminates the spiracular chamber. (Lovegrove et al., 2006)
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference Rac1^L89.Scer\UAS, Scer\GAL4^GMR.PF has neuroanatomy defective phenotype, enhanceable by bur^e10/bur[+] (Long et al., 2006)
Phenotype Manifest In
Enhanced by
Statement Reference Rac1^L89.Scer\UAS, Scer\GAL4^GMR.PF has photoreceptor cell & axon phenotype, enhanceable by bur^e10/bur[+] (Long et al., 2006)
Suppressed by
Statement Reference Rac1^L89.Scer\UAS has phenotype, suppressible by Scer\GAL80^αTub84B.PL/Scer\GAL4⁵⁴ (Lee and Luo, 1999)
Additional Comments
Genetic Interactions
Statement Reference The R-cell axon projection defects caused by expression of Rac1[L89.Scer\UAS] under the control of Scer\GAL4[GMR.PF] are enhanced by bur[e10]/+. (Long et al., 2006)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 2 )
Bloomington	6290 y¹ w^; P{UAS-Rac1.L89}6 5129 w^; P{GawB}LL54, P{UAS-Rac1.L89}6/CyO
Notes on Origin
Discoverer

Synonyms & Secondary IDs ( 6 )
Reported As
Symbol Synonym	DRac1^L89.6 (Paladi and Tepass, 2000) Drac1^L89 (Paululat et al., 1999) DRac^L.89 (Medioni and Noselli, 2005) Rac1^L89.Scer\UAS Rac1^L89.UAS Rac1^L89
Name Synonym
Secondary FlyBase IDs
	FBal0038991
References ( 11 )
Research paper	Long et al., 2006, Genetics 172(3): 1633--1642 De novo GMP synthesis is required for axon guidance in Drosophila. [FBrf0190740] Lovegrove et al., 2006, Curr. Biol. 16(22): 2206--2216 Coordinated control of cell adhesion, polarity, and cytoskeleton underlies Hox-induced organogenesis in Drosophila. [FBrf0194228] Medioni and Noselli, 2005, Development 132(13): 3069--3077 Dynamics of the basement membrane in invasive epithelial clusters in Drosophila. [FBrf0187473] Sepp and Auld, 2003, Development 130(9): 1825--1835 RhoA and Rac1 GTPases mediate the dynamic rearrangement of actin in peripheral glia. [FBrf0158880] Magie et al., 2002, Development 129(16): 3771--3782 Rho1 interacts with p120(ctn) and alpha-catenin, and regulates cadherin-based adherens junction components in Drosophila. [FBrf0151270] Lee and Luo, 1999, Neuron 22(3): 451--461 Mosaic analysis with a repressible neurotechnique cell marker for studies of gene function in neuronal morphogenesis. [FBrf0107846] Prokopenko et al., 1999, Genes Dev. 13(17): 2301--2314 A putative exchange factor for rho1 GTPase is required for initiation of cytokinesis in Drosophila. [FBrf0111464] Kaufmann et al., 1998, Development 125(3): 453--461 Drosophila Rac1 controls motor axon guidance. [FBrf0100716] Luo et al., 1994, Genes Dev. 8(15): 1787--1802 Distinct morphogenetic functions of similar small GTPases: Drosophila Drac1 is involved in axonal outgrowth and myoblast fusion. [FBrf0076490]
Review	Paululat et al., 1999, Mech. Dev. 83(1,2): 17--26 Essential genes for myoblast fusion in Drosophila embryogenesis. [FBrf0108990]
Abstract	Paladi and Tepass, 2000, A. Dros. Res. Conf. 41: 436C Requirement of Rho GTPases in the development and migration of embryonic hemocytes in Drosophila. [FBrf0126564]

Allele Dmel\Rac1L89.Scer\UAS

Allele Dmel\Rac1^L89.Scer\UAS