Allele Dmel\Rac1^Scer\UAS.cLa

General Information
Symbol	Dmel\Rac1^Scer\UAS.cLa	Species	D. melanogaster
Name	Saccharomyces cerevisiae UAS construct of Luo	FlyBase ID	FBal0038995
Feature type	allele	Associated gene	Dmel\Rac1

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02	Controlled Vocabulary Terms touch sensitivity defective sensory dendrite dorsal multidendritic neuron ddaA
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Luo et al., 1994)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Rac1 is expressed under the influence of Scer\GAL4 via upstream UAS sequences. (Luo et al., 1994)
Carried in construct	P{UAS-Rac1.L} (Younger, 2001.2, Hu et al., 2001, Kaufmann et al., 1998, Allan et al., 2003, Sepp and Auld, 2003, Hu et al., 2005, Geisbrecht and Montell, 2004, Luo et al., 1994, Sotillos and Campuzano, 2000, Zettervall et al., 2004, Emoto et al., 2004, Brand and Dormand, 1995, Allan et al., 2003, Srahna et al., 2006, Avet-Rochex et al., 2005, Leiss et al., 2009, Allen et al., 2000, Garlena et al., 2010, Tokusumi et al., 2009, Song and Giniger, 2011, Jinushi-Nakao et al., 2007, Ye et al., 2011, van Impel et al., 2009, Ball et al., 2010, Haralalka et al., 2011, Nagel et al., 2012, Tsubouchi et al., 2012)
Cytology
Phenotypic Data
Phenotypic Class
	lethal, with Scer\GAL4^109-79 (Younger, 2001.2) lethal, with Scer\GAL4^sd-SG29.1 (Sotillos and Campuzano, 2000) lethal \| larval stage, with Scer\GAL4^109-39 (Younger, 2001.2) lethal \| larval stage, with Scer\GAL4^how-24B (Luo et al., 1994) lethal \| pupal stage, with Scer\GAL4^GMR.PF (Avet-Rochex et al., 2005) neuroanatomy defective, with Scer\GAL4^109(2)80 (Andersen et al., 2005) neuroanatomy defective, with Scer\GAL4²²¹ (Jinushi-Nakao et al., 2007) neuroanatomy defective, with Scer\GAL4⁴⁷⁷ (Ye et al., 2011) neuroanatomy defective, with Scer\GAL4^A307 (Allen et al., 2000) neuroanatomy defective, with Scer\GAL4^ato.3.6 (Srahna et al., 2006) neuroanatomy defective \| adult stage, with Scer\GAL4^DB331 (Leiss et al., 2009) neuroanatomy defective \| larval stage, with Scer\GAL4^1003.3 (Tsubouchi et al., 2012) neuroanatomy defective \| third instar larval stage, with Scer\GAL4^BG380 (Ball et al., 2010) neuroanatomy defective \| third instar larval stage, with Scer\GAL4^elav-C155 (Ball et al., 2010) neuroanatomy defective \| third instar larval stage, with Scer\GAL4^Rapgap1-OK6 (Ball et al., 2010) neurophysiology defective, with Scer\GAL4^A307 (Allen et al., 2000) neurophysiology defective \| third instar larval stage, with Scer\GAL4^BG380 (Ball et al., 2010) neurophysiology defective \| third instar larval stage, with Scer\GAL4^Rapgap1-OK6 (Ball et al., 2010) size defective \| third instar larval stage, with Scer\GAL4^BG380 (Ball et al., 2010) size defective \| third instar larval stage, with Scer\GAL4^elav-C155 (Ball et al., 2010) size defective \| third instar larval stage, with Scer\GAL4^Rapgap1-OK6 (Ball et al., 2010) touch sensitivity defective \| larval stage, with Scer\GAL4^1003.3 (Tsubouchi et al., 2012) visible, with Scer\GAL4^en-e16E (Sotillos and Campuzano, 2000) visible, with Scer\GAL4^GMR.PF (Avet-Rochex et al., 2005)
Phenotype Manifest In
	axon & dorsal cluster neuron, with Scer\GAL4^ato.3.6 (Srahna et al., 2006) class I dendritic arborizing neuron, with Scer\GAL4²²¹ (Jinushi-Nakao et al., 2007) dendrite, with Scer\GAL4²²¹ (Jinushi-Nakao et al., 2007) dendrite, with Scer\GAL4^A307 (Allen et al., 2000) dendritic arborizing neuron, with Scer\GAL4⁴⁷⁷ (Ye et al., 2011) dendritic spine & lobular plate tangential neuron, with Scer\GAL4^DB331 (Leiss et al., 2009) dorsal multidendritic neuron ddaA \| larval stage, with Scer\GAL4^1003.3 (Tsubouchi et al., 2012) embryonic/larval neuromuscular junction \| third instar larval stage, with Scer\GAL4^BG380 (Ball et al., 2010) embryonic/larval neuromuscular junction \| third instar larval stage, with Scer\GAL4^elav-C155 (Ball et al., 2010) embryonic/larval neuromuscular junction \| third instar larval stage, with Scer\GAL4^Rapgap1-OK6 (Ball et al., 2010) eye, with Scer\GAL4^109-69 (Younger, 2001.2) eye, with Scer\GAL4^GMR.PF (Avet-Rochex et al., 2005) filopodium & dendrite, with Scer\GAL4^109(2)80 (Andersen et al., 2005) giant axon, with Scer\GAL4^A307 (Allen et al., 2000) hemocyte, with Scer\GAL4^He.PZ (Zettervall et al., 2004) hemocyte, with Scer\GAL4^Hml.PG (Zettervall et al., 2004) lamellocyte, with Scer\GAL4^He.PZ (Zettervall et al., 2004) lamellocyte, with Scer\GAL4^Hml.PG (Zettervall et al., 2004) macrochaeta, with Scer\GAL4^sca-109-68 (Younger, 2001.2) melanotic mass, with Scer\GAL4^He.PZ (Zettervall et al., 2004) melanotic mass, with Scer\GAL4^Hml.PG (Zettervall et al., 2004) multidendritic neuron & dendrite, with Scer\GAL4⁴⁷⁷ (Emoto et al., 2004) multidendritic neuron & dendrite \| supernumerary, with Scer\GAL4⁴⁷⁷ (Emoto et al., 2004) neurite, with Scer\GAL4^A307 (Allen et al., 2000) NMJ bouton \| third instar larval stage, with Scer\GAL4^BG380 (Ball et al., 2010) NMJ bouton \| third instar larval stage, with Scer\GAL4^elav-C155 (Ball et al., 2010) NMJ bouton \| third instar larval stage, with Scer\GAL4^Rapgap1-OK6 (Ball et al., 2010) ommatidium, with Scer\GAL4^GMR.PF (Hu et al., 2005) photoreceptor cell, with Scer\GAL4^GMR.PF (Hu et al., 2005) sensillum campaniformium, with Scer\GAL4^en-e16E (Sotillos and Campuzano, 2000) sensory dendrite \| larval stage, with Scer\GAL4^1003.3 (Tsubouchi et al., 2012) wing, with Scer\GAL4^en-e16E (Sotillos and Campuzano, 2000) wing vein, with Scer\GAL4^en-e16E (Sotillos and Campuzano, 2000)
Detailed Description
	Statement Reference Class III ddaA neurons expressing Rac1[Scer\UAS.cLa] under the control of Scer\GAL4[1003.3] show a dramatic increase in the number of dendritic sensory filopodia compared to wild type. These filopodia are dynamic and their stability is not altered compared to controls. The average length of the filopodia is significantly decreased compared to wild type, while the total length of primary dendritic branches is not altered. Larvae expressing Rac1[Scer\UAS.cLa] under the control of Scer\GAL4[1003.3] are hypersensitive to gentle touch compared to controls. (Tsubouchi et al., 2012) Overexpression of Rac1[Scer\UAS.cLa] in many neuron types, including the da neurons, under the control of Scer\GAL4[477], leads to hyperbranching of dendrites. (Ye et al., 2011) Expression of Rac1[Scer\UAS.cLa] under the control of the neuronal drivers Scer\GAL4[BG380], Scer\GAL4[OK6] or Scer\GAL4[elav-C155] leads to neuromuscular junction overgrowth during larval development, resulting in an increase in the number of synaptic boutons, branches and the appearance of abnormal synaptic protrusions. No overgrowth is seen when Rac1[Scer\UAS.cLa] is expressed in muscle under the control of the Scer\GAL4[Mhc.PW] driver. Expression of Rac1[Scer\UAS.cLa] under the control of either Scer\GAL4[BG380] or Scer\GAL4[OK6] produces increased mean evoked excitatory junctional potentials (EJPs) and quantal content in third instar larvae compared to controls. The size of miniature excitatory junctional potentials (mEJPs) does not differ from controls. (Ball et al., 2010) Expression of Rac1[Scer\UAS.cLa] under the control of Scer\GAL4[DB331] does not alter the overall dendritic architecture in the lobular plate tangential cells; neither the position not branching patterns of primary and secondary order dendrites are affected. However, there is an increase in spine density and a change in spine morphology (the spines appear shorter and less well defined) compared to controls. (Leiss et al., 2009) Expression of Rac1[Scer\UAS.cLa] under the control of Scer\GAL4[221] in class I neurons promotes de novo branch initiation reminiscent of, but not identical to, the filopodia of class III neurons. (Jinushi-Nakao et al., 2007) Expression of Rac1^Scer\UAS.cLa under the control of cer\GAL4^ato.3.6 inhibits the extension of axons from dorsal cluster neurons (DCNs), with an average of only 7.8 axons crossing toward the medulla instead of the wild-type average of 11.7. Expression of this transgene also results in a decreased number of DCN axons crossing the optic chiasm at 20%-30% pupal development. (Srahna et al., 2006) Expression of Rac1^Scer\UAS.cLa, under the regulation of Scer\GAL4^109(2)80, dramatic increases in larval neurons dendritic branching are observed. This is due to the formation of filopodia on normally non-filopodia bearing neurons (non-class III dendritic arborisation neurons), without altering the filopodia density. This branching phenotype becomes apparent in newly-hatched first instar larvae. Rac1^Scer\UAS.cLa expression, under the regulation of Scer\GAL4^109(2)80, like CaMKII^{T287D.Scer\UAS} expression, increases actin turnover, although the effects on morphological stability are significantly different. (Andersen et al., 2005) Expression of Rac1[Scer\UAS.cLa] under the control of Scer\GAL4[GMR.PF] results in about 90% pupal lethality. Escaper adults have strongly reduced eyes with no organised structure. (Avet-Rochex et al., 2005) When Rac1^Scer\UAS.cLa is driven by Scer\GAL4^GMR.PF a rough eye phenotype is seen. (Hu et al., 2005) When Rac1^Scer\UAS.cLa is driven by Scer\GAL4⁴⁷⁷, an overbranching phenotype is seen in class IV neurons. (Emoto et al., 2004) Expression of Rac1^Scer\UAS.cLa under the control of Scer\GAL4^slbo.2.6 at 25^oC does not alter the migration of border follicle cells that occurs during oogenesis. (Geisbrecht and Montell, 2004) When expression is driven by Scer\GAL4^He.PZ, hemocyte proliferation and lamellocyte numbers are increased, and crystal cells are unaffected. Melanotic masses form. Similar, though milder, effects are seen when expression is driven by Scer\GAL4^Hml.PG. (Zettervall et al., 2004) When driven by Scer\GAL4^sca-109-68 bristle defects occur. When driven by Scer\GAL4^109-69 eyes are roughened. Lethality, when driven by Scer\GAL4^109-79 acts in the embryonic-larval stages. (Younger, 2001.2) Targeted expression of Rac1[Scer\UAS.cLa] to the GF under the control of Scer\GAL4[A307] results in over 95% of GF axons showing morphological aberrations whereas less than 15% of controls show defects. In the majority of specimens the axons grow out of the brain and into the second thoracic neuromere (T2) but fail to make the terminal bend that is characteristic of the gf presynatpic terminal. In addition the tips of the axons appear swollen and contain vesicle-like structures. Nine percent of axons fail to exit the brain and often cross the midline and project into the contralateral half of the brain. Over 90% of GFs in Scer\GAL4[A307]; Rac1[Scer\UAS.cLa] adult brains exhibit abnormalities. Although neurite outgrowth and branching appears normal, and the three major dendritic domains are always discernible, typically dendrite are thickened and display inappropriate minor branches. Swollen dendrites containing large vesicle-like structures are also observed. When Scer\GAL4[A307]; Rac1[Scer\UAS.cLa] flies are tested physiologically, they all exhibit a TTM response latency that is more than twice that of control flies. The fidelity of transmission for repetitive stimuli is also reduced and in most cases only a single response to the first stimulus occurs. Approximately 30% of Scer\GAL4[c17]; Rac1[Scer\UAS.cLa] GF axons exhibit a bendless phenotype with swollen terminals and no lateral bends while the remaining axons appear wild-type. Approximately 81% of Scer\GAL4[A307]; Rac1[Scer\UAS.cLa] flies exhibit a bendless GF phenotype, while 64% of Scer\GAL4[A307]; Rac1[Scer\UAS.cLa] GFs are bendless. The remainder exhibit wild-type bends or large abnormally shaped processes in the target region. (Allen et al., 2000) Expression is driven by Scer\GAL4^en-e16E causes wing vein thickening, extra sensory organs and wing enlargement. (Sotillos and Campuzano, 2000) Scer\GAL4^elav-C155-mediated expression does not affect the normal anatomy of the CNS pathways. Scer\GAL4^elav-C155-mediated expression of Rac1^L89.Scer\UAS slightly disrupts CNS axon pathway, gaps between segments suggest failure in axon extension. Coexpression of Rac1^Scer\UAS.cLa under the same Scer\GAL4 driver causes a massive failure in axon extension, dramatically enhancing the effect of Rac1^L89.Scer\UAS. Axons that do extend follow abnormal trajectories. (Kaufmann et al., 1998)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference Rac1^Scer\UAS.cLa, Scer\GAL4²²¹ has neuroanatomy defective phenotype, enhanceable by ct^Scer\UAS.cPa, Scer\GAL4²²¹ (Jinushi-Nakao et al., 2007) Rac1^Scer\UAS.cLa, Scer\GAL4^sd-SG29.1 has visible phenotype, enhanceable by Scer\GAL4^en-e16E/tum^{ΔE1E.Scer\UAS} (Sotillos and Campuzano, 2000)
NOT Enhanced by
Statement Reference Rac1^Scer\UAS.cLa, Scer\GAL4^ato.3.6 has neuroanatomy defective phenotype, non-enhanceable by btl^DN.Scer\UAS, Scer\GAL4^ato.3.6 (Srahna et al., 2006)
Suppressed by
Statement Reference Rac1^Scer\UAS.cLa, Scer\GAL4^BG380 has neurophysiology defective \| third instar larval stage phenotype, suppressible by wit^HA3/wit^A12 (Ball et al., 2010) Rac1^Scer\UAS.cLa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has lethal \| pupal stage phenotype, suppressible \| partially by Zzzz\ExoS^GAP.Scer\UAS, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Avet-Rochex et al., 2005) Rac1^Scer\UAS.cLa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has visible phenotype, suppressible \| partially by Zzzz\ExoS^GAP.Scer\UAS, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Avet-Rochex et al., 2005) Rac1^Scer\UAS.cLa, Scer\GAL4^Rapgap1-OK6 has neuroanatomy defective \| third instar larval stage phenotype, suppressible \| partially by trio^S137203/trio[+] (Ball et al., 2010) Rac1^Scer\UAS.cLa, Scer\GAL4^Rapgap1-OK6 has neuroanatomy defective \| third instar larval stage phenotype, suppressible by trio^S137203 (Ball et al., 2010) Rac1^Scer\UAS.cLa, Scer\GAL4^Rapgap1-OK6 has neurophysiology defective \| third instar larval stage phenotype, suppressible by trio^6A/trio^S137203 (Ball et al., 2010) Rac1^Scer\UAS.cLa, Scer\GAL4^Rapgap1-OK6 has size defective \| third instar larval stage phenotype, suppressible \| partially by trio^S137203/trio[+] (Ball et al., 2010) Rac1^Scer\UAS.cLa, Scer\GAL4^Rapgap1-OK6 has size defective \| third instar larval stage phenotype, suppressible by trio^S137203 (Ball et al., 2010) Rac1^Scer\UAS.cLa, Scer\GAL4^sd-SG29.1 has lethal phenotype, suppressible by tum^Scer\UAS.cSa, Scer\GAL4^sd-SG29.1 (Sotillos and Campuzano, 2000)
NOT suppressed by
Statement Reference Rac1^Scer\UAS.cLa, Scer\GAL4⁴⁷⁷ has neuroanatomy defective phenotype, non-suppressible by dar1³⁰¹⁰ (Ye et al., 2011) Rac1^Scer\UAS.cLa, Scer\GAL4^ato.3.6 has neuroanatomy defective phenotype, non-suppressible by btl^DN.Scer\UAS, Scer\GAL4^ato.3.6 (Srahna et al., 2006) Rac1^Scer\UAS.cLa, Scer\GAL4^Rapgap1-OK6 has neuroanatomy defective \| third instar larval stage phenotype, non-suppressible by sif^ES11 (Ball et al., 2010)
Enhancer of
Statement Reference Rac1^Scer\UAS.cLa, Scer\GAL4²²¹ is an enhancer of neuroanatomy defective phenotype of Scer\GAL4²²¹, ct^Scer\UAS.cPa (Jinushi-Nakao et al., 2007) Rac1^Scer\UAS.cLa, Scer\GAL4²²¹ is an enhancer of neuroanatomy defective phenotype of Scer\GAL4²²¹, kn^Scer\UAS.cMa (Jinushi-Nakao et al., 2007) Rac1^Scer\UAS.cLa is an enhancer of visible \| adult stage phenotype of Scer\GAL4^GMR.PU, pbl^{DH-PH.Scer\UAS.T:Ivir\HA1} (van Impel et al., 2009)
NOT Enhancer of
Statement Reference Scer\GAL4⁶⁰/Rac1^Scer\UAS.cLa is a non-enhancer of neuroanatomy defective \| heat sensitive phenotype of N^l1N-ts1 (Song and Giniger, 2011)
Suppressor of
Statement Reference Rac1^Scer\UAS.cLa, Scer\GAL4^ato.3.6 is a suppressor of neuroanatomy defective phenotype of Scer\GAL4^ato.3.6, btl^DN.Scer\UAS (Srahna et al., 2006) Rac1^Scer\UAS.cLa, Scer\GAL4^en-e16E is a suppressor \| partially of visible phenotype of Scer\GAL4^en-e16E, phl^{K497M.Scer\UAS} (Sotillos and Campuzano, 2000) Rac1^Scer\UAS.cLa is a suppressor of cell migration defective \| embryonic stage phenotype of Scer\GAL4^twi.2PE, pbl^{ΔBRCT.Scer\UAS.T:Hsap\MYC}, pbl³ (van Impel et al., 2009)
NOT Suppressor of
Statement Reference Scer\GAL4^BG380/Rac1^Scer\UAS.cLa is a non-suppressor of neurophysiology defective \| third instar larval stage phenotype of wit^HA3/wit^A12 (Ball et al., 2010)
Other
Statement Reference Rac1^Scer\UAS.cLa, Scer\GAL4^GMR.PU, pbl^{DH-PH.Scer\UAS.T:Ivir\HA1} has partially lethal - majority die \| pharate adult stage phenotype (van Impel et al., 2009)
Phenotype Manifest In
Enhanced by
Statement Reference Rac1^Scer\UAS.cLa, Scer\GAL4²²¹ has class I dendritic arborizing neuron phenotype, enhanceable by ct^Scer\UAS.cPa, Scer\GAL4²²¹ (Jinushi-Nakao et al., 2007) Rac1^Scer\UAS.cLa, Scer\GAL4²²¹ has dendrite phenotype, enhanceable by ct^Scer\UAS.cPa, Scer\GAL4²²¹ (Jinushi-Nakao et al., 2007) Rac1^Scer\UAS.cLa, Scer\GAL4^sd-SG29.1 has sensillum campaniformium phenotype, enhanceable by Scer\GAL4^en-e16E/tum^{ΔE1E.Scer\UAS} (Sotillos and Campuzano, 2000) Rac1^Scer\UAS.cLa, Scer\GAL4^sd-SG29.1 has wing phenotype, enhanceable by Scer\GAL4^en-e16E/tum^{ΔE1E.Scer\UAS} (Sotillos and Campuzano, 2000) Rac1^Scer\UAS.cLa, Scer\GAL4^sd-SG29.1 has wing vein phenotype, enhanceable by Scer\GAL4^en-e16E/tum^{ΔE1E.Scer\UAS} (Sotillos and Campuzano, 2000)
NOT Enhanced by
Statement Reference Rac1^Scer\UAS.cLa, Scer\GAL4^ato.3.6 has axon & dorsal cluster neuron phenotype, non-enhanceable by btl^DN.Scer\UAS, Scer\GAL4^ato.3.6 (Srahna et al., 2006)
Suppressed by
Statement Reference Rac1^Scer\UAS.cLa, Scer\GAL4⁴⁷⁷ has multidendritic neuron & dendrite \| supernumerary phenotype, suppressible \| partially by trc^{Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4⁴⁷⁷ (Emoto et al., 2004) Rac1^Scer\UAS.cLa, Scer\GAL4⁴⁷⁷ has multidendritic neuron & dendrite phenotype, suppressible \| partially by trc^{Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4⁴⁷⁷ (Emoto et al., 2004) Rac1^Scer\UAS.cLa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has eye phenotype, suppressible \| partially by Zzzz\ExoS^GAP.Scer\UAS, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Avet-Rochex et al., 2005) Rac1^Scer\UAS.cLa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has ommatidium phenotype, suppressible by RhoGAP93B^Scer\UAS.cHa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Hu et al., 2005) Rac1^Scer\UAS.cLa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has photoreceptor cell phenotype, suppressible by RhoGAP93B^Scer\UAS.cHa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Hu et al., 2005) Rac1^Scer\UAS.cLa, Scer\GAL4^Rapgap1-OK6 has embryonic/larval neuromuscular junction \| third instar larval stage phenotype, suppressible \| partially by trio^S137203/trio[+] (Ball et al., 2010) Rac1^Scer\UAS.cLa, Scer\GAL4^Rapgap1-OK6 has embryonic/larval neuromuscular junction \| third instar larval stage phenotype, suppressible by trio^S137203 (Ball et al., 2010) Rac1^Scer\UAS.cLa, Scer\GAL4^Rapgap1-OK6 has NMJ bouton \| third instar larval stage phenotype, suppressible \| partially by trio^S137203/trio[+] (Ball et al., 2010) Rac1^Scer\UAS.cLa, Scer\GAL4^Rapgap1-OK6 has NMJ bouton \| third instar larval stage phenotype, suppressible by trio^S137203 (Ball et al., 2010)
NOT suppressed by
Statement Reference Rac1^Scer\UAS.cLa, Scer\GAL4⁴⁷⁷ has dendritic arborizing neuron phenotype, non-suppressible by dar1³⁰¹⁰ (Ye et al., 2011) Rac1^Scer\UAS.cLa, Scer\GAL4^ato.3.6 has axon & dorsal cluster neuron phenotype, non-suppressible by btl^DN.Scer\UAS, Scer\GAL4^ato.3.6 (Srahna et al., 2006) Rac1^Scer\UAS.cLa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has ommatidium phenotype, non-suppressible by Rho1^{dsRNA.Scer\UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Hu et al., 2005) Rac1^Scer\UAS.cLa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has photoreceptor cell phenotype, non-suppressible by Rho1^{dsRNA.Scer\UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Hu et al., 2005) Rac1^Scer\UAS.cLa, Scer\GAL4^Rapgap1-OK6 has embryonic/larval neuromuscular junction \| third instar larval stage phenotype, non-suppressible by sif^ES11 (Ball et al., 2010) Rac1^Scer\UAS.cLa, Scer\GAL4^Rapgap1-OK6 has NMJ bouton \| third instar larval stage phenotype, non-suppressible by sif^ES11/sif[+] (Ball et al., 2010)
Enhancer of
Statement Reference Rac1^Scer\UAS.cLa, Scer\GAL4²²¹ is an enhancer of class I dendritic arborizing neuron phenotype of Scer\GAL4²²¹, ct^Scer\UAS.cPa (Jinushi-Nakao et al., 2007) Rac1^Scer\UAS.cLa, Scer\GAL4²²¹ is an enhancer of class I dendritic arborizing neuron phenotype of Scer\GAL4²²¹, kn^Scer\UAS.cMa (Jinushi-Nakao et al., 2007) Rac1^Scer\UAS.cLa, Scer\GAL4²²¹ is an enhancer of dendrite phenotype of Scer\GAL4²²¹, ct^Scer\UAS.cPa (Jinushi-Nakao et al., 2007) Rac1^Scer\UAS.cLa, Scer\GAL4²²¹ is an enhancer of dendrite phenotype of Scer\GAL4²²¹, kn^Scer\UAS.cMa (Jinushi-Nakao et al., 2007) Rac1^Scer\UAS.cLa is an enhancer of eye \| adult stage phenotype of Scer\GAL4^GMR.PU, pbl^{DH-PH.Scer\UAS.T:Ivir\HA1} (van Impel et al., 2009)
NOT Enhancer of
Statement Reference Scer\GAL4⁶⁰/Rac1^Scer\UAS.cLa is a non-enhancer of intersegmental nerve \| heat sensitive phenotype of N^l1N-ts1 (Song and Giniger, 2011) Scer\GAL4⁶⁰/Rac1^Scer\UAS.cLa is a non-enhancer of intersegmental nerve branch ISNb of A1-7 \| heat sensitive phenotype of N^l1N-ts1 (Song and Giniger, 2011)
Suppressor of
Statement Reference Rac1^Scer\UAS.cLa, Scer\GAL4^ato.3.6 is a suppressor of axon & dorsal cluster neuron & adult brain phenotype of Scer\GAL4^ato.3.6, btl^DN.Scer\UAS (Srahna et al., 2006) Rac1^Scer\UAS.cLa, Scer\GAL4^elav.PLu is a suppressor of intersegmental nerve phenotype of Scer\GAL4^elav.PLu, plexB^Scer\UAS.cHa (Hu et al., 2001) Rac1^Scer\UAS.cLa, Scer\GAL4^elav.PLu is a suppressor of RP3 motor neuron phenotype of Scer\GAL4^elav.PLu, plexB^Scer\UAS.cHa (Hu et al., 2001) Rac1^Scer\UAS.cLa, Scer\GAL4^en-e16E is a suppressor \| partially of wing phenotype of Scer\GAL4^en-e16E, phl^{K497M.Scer\UAS} (Sotillos and Campuzano, 2000) Rac1^Scer\UAS.cLa, Scer\GAL4^en-e16E is a suppressor \| partially of wing vein L4 phenotype of Scer\GAL4^en-e16E, phl^{K497M.Scer\UAS} (Sotillos and Campuzano, 2000) Rac1^Scer\UAS.cLa, Scer\GAL4^en-e16E is a suppressor \| partially of wing vein L5 phenotype of Scer\GAL4^en-e16E, phl^{K497M.Scer\UAS} (Sotillos and Campuzano, 2000) Rac1^Scer\UAS.cLa is a suppressor of mesoderm phenotype of Scer\GAL4^twi.2PE, pbl^{ΔBRCT.Scer\UAS.T:Hsap\MYC}, pbl³ (van Impel et al., 2009)
NOT Suppressor of
Statement Reference Scer\GAL4^sns.PK/Rac1^Scer\UAS.cLa is a non-suppressor of embryonic myoblast phenotype of mbc^D11.2 (Haralalka et al., 2011)
Additional Comments
Genetic Interactions
Statement Reference Expression of Rac1[Scer\UAS.cLa] under the control of Scer\GAL4[sns.PK] does not rescue myoblast fusion in homozygous mbc[D11.2] embryos. (Haralalka et al., 2011) The ISNb bypass phenotype of N[l1N-ts1] mutant embryos is not significantly modulated by expression of Rac1[Scer\UAS.cLa] under the control of Scer\GAL4[60]. (Song and Giniger, 2011) A dar1[3010] mutant background does not block the dendrite branching activity found upon expression of Rac1[Scer\UAS.cLa] under the control of Scer\GAL4[477]. (Ye et al., 2011) One copy of trio[S137203] partially suppresses the larval neuromuscular junction overgrowth and bouton number phenotypes seen when Rac1[Scer\UAS.cLa] is expressed in neurons under the control of Scer\GAL4[OK6]. Bouton number is reduced by more than 50%. One copy of sif[ES11] is unable to suppress the increase in larval neuromuscular junction bouton number seen when Rac1[Scer\UAS.cLa] is expressed in neurons under the control of Scer\GAL4[OK6]. Homozygous trio[S137203] suppresses the larval neuromuscular junction overgrowth and increase in bouton number seen when Rac1[Scer\UAS.cLa] is expressed in neurons under the control of Scer\GAL4[OK6]. wit[A12]/wit[HA3] suppresses the increase in evoked excitatory junctional potential (EJPs) and quantal content seen when Rac1[Scer\UAS.cLa] is expressed in motor neurons under the control of Scer\GAL4[BG380], with third instar larvae instead displaying the reduction in neurotransmitter release phenotype seen in wit[A12]/wit[HA3] mutants. trio[S137203]/trio[6A] suppresses the increase in evoked excitatory junctional potential (EJPs) and quantal content seen when Rac1[Scer\UAS.cLa] is expressed in neurons under the control of Scer\GAL4[OK6]. (Ball et al., 2010) Co-expression of Rac1[Scer\UAS.cLa] enhances the partial rescue of pbl[3] mesodermal migration defects seen when pbl[ΔBRCT.Scer\UAS.T:Hsap\MYC] is expressed under the control of Scer\GAL4[twi.2PE]. Co-expression of Rac1[Scer\UAS.cLa] strongly enhances the eye phenotype seen when pbl[DH-PH.Scer\UAS.T:Ivir\HA1] is expressed under the control of Scer\GAL4[GMR.PU]. Most flies die as pharate adults but escapers fail to develop any eye structures. (van Impel et al., 2009) Ectopic expression of Rac1[Scer\UAS.cLa] along with ct[Scer\UAS.cPa] in class I neurons under the control of Scer\GAL4[221] leads to the formation of an arbor with many filpodia/spikes, which shows a significant enhancement of branching as compared to either Rac1[Scer\UAS.cLa] or ct[Scer\UAS.cPa] expression alone. Ectopic expression of Rac1[Scer\UAS.cLa] along with kn[Scer\UAS.cMa] in class I neurons under the control of Scer\GAL4[221] promotes the outgrowth of bona fide dendrites from the thorn-shaped projections initiated by Rac1[Scer\UAS.cLa] expression. In addition, coexpression of kn[Scer\UAS.cMa] causes a small, significant reduction in termini number. (Jinushi-Nakao et al., 2007) Coexpression of Rac1^Scer\UAS.cLa and btl^DN.Scer\UAS, under the control of Scer\GAL4^ato.3.6, results in the dominance of the Rac1^Scer\UAS.cLa phenotype, with a reduction in the number of dorsal cluster neuron axons crossing toward the medulla. This indicates that the Rac1^N17.Scer\UAS phenotype is dominant. (Srahna et al., 2006) the addition of Rac1^Scer\UAS.cLa to plexB^Scer\UAS.cHa, Scer\GAL4^elav.PLu flies suppresses the Intersegmental nerve and RP3 nerve phenotypes. (Hu et al., 2001)
Xenogenetic Interactions
Statement Reference The viability of flies expressing Rac1[Scer\UAS.cLa] under the control of Scer\GAL4[GMR.PF] is enhanced to 50% by co-expression of Zzzz\ExoS[GAP.Scer\UAS]. In addition, the eye morphology of the surviving adults is restored towards wild type. (Avet-Rochex et al., 2005)
Complementation & Rescue Data
Partially rescues	Scer\GAL4^elav-C155/Rac1^Scer\UAS.cLa partially rescues Rac1^N17.Scer\UAS (Kaufmann et al., 1998) Scer\GAL4^slbo.2.6/Rac1^Scer\UAS.cLa partially rescues Rac1^N17.Scer\UAS (Geisbrecht and Montell, 2004)
Comments	Expression of Rac1[Scer\UAS.cLa] under the control of Scer\GAL4[sns.PK] strongly rescues the myoblast fusion defects of Rac1[J11] Rac2[Δ] double mutant embryos, resulting in a near normal somatic muscle pattern. In contrast, expression of Rac1[Scer\UAS.cLa] under the control of Scer\GAL4[kirre-rP298] rescues the myoblast fusion defects of Rac1[J11] Rac2[Δ] double mutant embryos much less efficiently. (Haralalka et al., 2011) The border follicle cell migration defect seen in egg chambers expressing Rac1^N17.Scer\UAS under the control of Scer\GAL4^slbo.2.6 is partially suppressed by coexpression of Rac1^Scer\UAS.cLa. (Geisbrecht and Montell, 2004) Restores CNS pathways to near normal morphology, some abnormalities can be seen in occasional segments. (Kaufmann et al., 1998)
Stocks ( 2 )
Bloomington	6293 w^*; P{UAS-Rac1.L}3
Kyoto	108726 y¹ w^*; P{UAS-Rac1.L}3
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	Rac1^Scer\UAS.cLa Rac1^UAS.cLa
Name Synonym	Saccharomyces cerevisiae UAS construct of Luo (Luo et al., 1994)
Secondary FlyBase IDs

References ( 28 )

Generate a list of	All references relating to this allele ( 28 )

List References by type	Research paper ( 25 ) Supplementary material ( 1 ) Review ( 1 ) Personal communication to FlyBase ( 1 )
Recent research papers ( 5 )
	Nagel et al., 2012, Development 139(16): 2999--3009 Fascin controls neuronal class-specific dendrite arbor morphology. [FBrf0219030] Tsubouchi et al., 2012, Curr. Biol. 22(22): 2124--2134 Dendritic Filopodia, Ripped Pocket, NOMPC, and NMDARs Contribute to the Sense of Touch in Drosophila Larvae. [FBrf0219946] Haralalka et al., 2011, Development 138(8): 1551--1562 Asymmetric Mbc, active Rac1 and F-actin foci in the fusion-competent myoblasts during myoblast fusion in Drosophila. [FBrf0213340] Song and Giniger, 2011, Dev. Dyn. 240(2): 324--332 Noncanonical notch function in motor axon guidance is mediated by Rac GTPase and the GEF1 domain of trio. [FBrf0212819] Ye et al., 2011, J. Neurosci. 31(9): 3309--3319 Differential regulation of dendritic and axonal development by the novel kruppel-like factor dar1. [FBrf0213162] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...

Allele Dmel\Rac1Scer\UAS.cLa

Allele Dmel\Rac1^Scer\UAS.cLa