FB2025_01 , released February 20, 2025
Allele: Dmel\Rac1UAS.cLa
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General Information
Symbol
Dmel\Rac1UAS.cLa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct of Luo
FlyBase ID
FBal0038995
Feature type
allele
Associated gene
Dmel\Rac1
Associated Insertion(s)
Carried in Construct
P{UAS-Rac1.L}
Also Known As
UAS-Rac1, UAS-rac, UAS-DRac1, UAS-rac1.L, UAS-Rac1wt
Key Links
Transgenic product class
cDNA
(Luo et al., 1994)
wild_type
(Luo et al., 1994)
Mutagen
Nature of the Allele
Transgenic product class
cDNA
(Luo et al., 1994)
wild_type
(Luo et al., 1994)
Mutagen
in vitro construct
(Luo et al., 1994)
Progenitor genotype
Carried in construct
P{UAS-Rac1.L}
Cytology
Description

UASt regulatory sequences drive expression of a Rac1 cDNA.

(Luo et al., 1994)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Rac1
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      ameliorates  Machado-Joseph disease
      modeled by Hsap\ATXN3tr.Q78.UAS.Tag:HA
      (Lee et al., 2011)
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class

      abnormal cell shape, with Scer\GAL4Cg.PU

      (Molina et al., 2022)

      abnormal circadian behavior, with Scer\GAL4P0.5.Pdf

      (Petsakou et al., 2015)

      abnormal neuroanatomy, with Scer\GAL4109(2)80

      (Andersen et al., 2005)

      abnormal neuroanatomy, with Scer\GAL4221

      (Jinushi-Nakao et al., 2007)

      abnormal neuroanatomy, with Scer\GAL4477

      (Ye et al., 2011)

      abnormal neuroanatomy, with Scer\GAL4ato.3.6

      (Srahna et al., 2006)

      abnormal neuroanatomy, with Scer\GAL4P0.5.Pdf

      (Petsakou et al., 2015)

      abnormal neuroanatomy, with Scer\GAL4shot-OK307

      (Allen et al., 2000)

      abnormal neuroanatomy | adult stage, with Scer\GAL4DB331

      (Leiss et al., 2009)

      abnormal neuroanatomy | larval stage, with Scer\GAL41003.3

      (Tsubouchi et al., 2012)

      abnormal neuroanatomy | larval stage, with Scer\GAL4ppk.PG

      (Lin et al., 2015, Lee et al., 2011)

      abnormal neuroanatomy | third instar larval stage, with Scer\GAL4109(2)80

      (Bagley et al., 2014)

      abnormal neuroanatomy | third instar larval stage, with Scer\GAL4BG380

      (Ball et al., 2010)

      abnormal neuroanatomy | third instar larval stage, with Scer\GAL4elav-C155

      (Ball et al., 2010)

      abnormal neuroanatomy | third instar larval stage, with Scer\GAL4RapGAP1-OK6

      (Ball et al., 2010)

      abnormal neurophysiology, with Scer\GAL4shot-OK307

      (Allen et al., 2000)

      abnormal neurophysiology | third instar larval stage, with Scer\GAL4BG380

      (Ball et al., 2010)

      abnormal neurophysiology | third instar larval stage, with Scer\GAL4RapGAP1-OK6

      (Ball et al., 2010)

      abnormal size | third instar larval stage, with Scer\GAL4BG380

      (Ball et al., 2010)

      abnormal size | third instar larval stage, with Scer\GAL4elav-C155

      (Ball et al., 2010)

      abnormal size | third instar larval stage, with Scer\GAL4RapGAP1-OK6

      (Ball et al., 2010)

      abnormal touch response | larval stage, with Scer\GAL41003.3

      (Tsubouchi et al., 2012)

      increased cell growth rate | second instar larval stage | conditional, with Scer\GAL4ppk.PU

      (Song et al., 2015)

      increased cell number | larval stage, with Scer\GAL4He.PZ

      (Zettervall et al., 2004)

      increased cell number | larval stage, with Scer\GAL4Hml.PG

      (Zettervall et al., 2004)

      lethal, with Scer\GAL4109-79

      (Younger, 2001.2)

      lethal, with Scer\GAL4sd-SG29.1

      (Sotillos and Campuzano, 2000)

      lethal - all die before end of larval stage, with Scer\GAL4109-39

      (Younger, 2001.2)

      lethal - all die before end of larval stage, with Scer\GAL4how-24B

      (Luo et al., 1994)

      lethal - all die before end of pupal stage, with Scer\GAL4GMR.PF

      (Avet-Rochex et al., 2005)

      some die during larval stage, with Scer\GAL4109-39

      (Younger, 2001.2)

      some die during larval stage, with Scer\GAL4how-24B

      (Luo et al., 1994)

      some die during pupal stage, with Scer\GAL4GMR.PF

      (Avet-Rochex et al., 2005)

      visible, with Scer\GAL4en-e16E

      (Sotillos and Campuzano, 2000)

      visible, with Scer\GAL4GMR.PF

      (Avet-Rochex et al., 2005)
      Phenotype Manifest In

      axon, with Scer\GAL4elav.PLu

      (Banka et al., 2022)

      axon & dorsal cluster neuron, with Scer\GAL4ato.3.6

      (Srahna et al., 2006)

      campaniform sensillum, with Scer\GAL4en-e16E

      (Sotillos and Campuzano, 2000)

      dendrite, with Scer\GAL4221

      (Jinushi-Nakao et al., 2007)

      dendrite, with Scer\GAL4shot-OK307

      (Allen et al., 2000)

      dendrite | third instar larval stage, with Scer\GAL4109(2)80

      (Bagley et al., 2014)

      dendritic arborizing neuron, with Scer\GAL4477

      (Ye et al., 2011)

      dendritic spine & lobular plate tangential neuron, with Scer\GAL4DB331

      (Leiss et al., 2009)

      embryonic/larval neuromuscular junction | third instar larval stage, with Scer\GAL4BG380

      (Ball et al., 2010)

      embryonic/larval neuromuscular junction | third instar larval stage, with Scer\GAL4elav-C155

      (Ball et al., 2010)

      embryonic/larval neuromuscular junction | third instar larval stage, with Scer\GAL4RapGAP1-OK6

      (Ball et al., 2010)

      eye, with Scer\GAL4109-69

      (Younger, 2001.2)

      eye, with Scer\GAL4GMR.PF

      (Avet-Rochex et al., 2005)

      filamentous actin, with Scer\GAL4ppk.PG

      (Lee et al., 2011)

      filopodium & dendrite, with Scer\GAL4109(2)80

      (Andersen et al., 2005)

      filopodium | increased number, with Scer\GAL4elav.PLu

      (Banka et al., 2022)

      giant axon, with Scer\GAL4shot-OK307

      (Allen et al., 2000)

      hemocyte, with Scer\GAL4Cg.PU

      (Molina et al., 2022)

      hemocyte, with Scer\GAL4He.PZ

      (Zettervall et al., 2004)

      hemocyte, with Scer\GAL4Hml.PG

      (Zettervall et al., 2004)

      interommatidial cell, with Scer\GAL4GMR.long

      (Malin et al., 2024)

      interommatidial cell | somatic clone, with Scer\GAL4Tub.PU

      (Malin et al., 2024)

      lamellocyte, with Scer\GAL4He.PZ

      (Zettervall et al., 2004)

      lamellocyte, with Scer\GAL4Hml.PG

      (Zettervall et al., 2004)

      larval dorsal multidendritic neuron ddaA | larval stage, with Scer\GAL41003.3

      (Tsubouchi et al., 2012)

      larval multidendritic class I neuron, with Scer\GAL4221

      (Jinushi-Nakao et al., 2007)

      larval multidendritic class I neuron | third instar larval stage, with Scer\GAL4109(2)80

      (Bagley et al., 2014)

      larval multidendritic class IV neuron, with Scer\GAL4ppk.PG

      (Lin et al., 2015)

      lobula columnar neuron LC14 | larval stage, with Scer\GAL4ppk.PG

      (Lee et al., 2011)

      macrochaeta, with Scer\GAL4sca-109-68

      (Younger, 2001.2)

      melanotic mass, with Scer\GAL4He.PZ

      (Zettervall et al., 2004)

      melanotic mass, with Scer\GAL4Hml.PG

      (Zettervall et al., 2004)

      multidendritic neuron & dendrite, with Scer\GAL4477

      (Emoto et al., 2004)

      multidendritic neuron & dendrite | supernumerary, with Scer\GAL4477

      (Emoto et al., 2004)

      neurite, with Scer\GAL4shot-OK307

      (Allen et al., 2000)

      NMJ bouton | third instar larval stage, with Scer\GAL4BG380

      (Ball et al., 2010)

      NMJ bouton | third instar larval stage, with Scer\GAL4elav-C155

      (Ball et al., 2010)

      NMJ bouton | third instar larval stage, with Scer\GAL4RapGAP1-OK6

      (Ball et al., 2010)

      ommatidium, with Scer\GAL4GMR.long

      (Malin et al., 2024)

      ommatidium, with Scer\GAL4GMR.PF

      (Hu et al., 2005)

      ommatidium | somatic clone, with Scer\GAL4Tub.PU

      (Malin et al., 2024)

      photoreceptor neuron, with Scer\GAL4GMR.PF

      (Hu et al., 2005)

      sensory dendrite | larval stage, with Scer\GAL41003.3

      (Tsubouchi et al., 2012)

      s-LNv Pdf neuron, with Scer\GAL4P0.5.Pdf

      (Petsakou et al., 2015)

      wing, with Scer\GAL4en-e16E

      (Sotillos and Campuzano, 2000)

      wing vein, with Scer\GAL4en-e16E

      (Sotillos and Campuzano, 2000)
      Detailed Description
      Statement
      Reference

      Scer\GAL4ppk.PG-mediated overexpression of Rac1Scer\UAS.cLa drives ectopic dendrite branching, although total dendrite length is unaffected. This increase in terminal dendrite branching is accompanied by a significant decrease in primary dendrite growth - as a result, distal portions of the territory normally covered by C4da neurons are completely devoid of dendrites.

      (Lin et al., 2015)

      Expression of Rac1Scer\UAS.cLa for 12 hours under the control of Scer\GAL4P0.5.Pdf (restricted to the adult stages using Scer\GAL80ts.αTub84B) significantly increases the spread of s-LNv projections in the x and y axes at dawn (ZT24).

      Expression of Rac1Scer\UAS.cLa under the control of Scer\GAL4P0.5.Pdf lengthens the behavioral rhythm period to 25 hours.

      (Petsakou et al., 2015)

      Overexpressing Rac1Scer\UAS.cLa in class IV da neurons (Scer\GAL4ppk.PU) partially (increased regeneration percentage but not terminal branching or commissure growth) improves axon regeneration.

      (Song et al., 2015)

      Overexpression of Rac1Scer\UAS.cLa driven by Scer\GAL4109(2)80 leads to a significant increase in dendrite branch points (but not length) in class I da sensory neurons compared to wild type.

      (Bagley et al., 2014)

      Class III ddaA neurons expressing Rac1Scer\UAS.cLa under the control of Scer\GAL41003.3 show a dramatic increase in the number of dendritic sensory filopodia compared to wild type. These filopodia are dynamic and their stability is not altered compared to controls. The average length of the filopodia is significantly decreased compared to wild type, while the total length of primary dendritic branches is not altered.

      Larvae expressing Rac1Scer\UAS.cLa under the control of Scer\GAL41003.3 are hypersensitive to gentle touch compared to controls.

      (Tsubouchi et al., 2012)

      Expression of Rac1Scer\UAS.cLa under the control of Scer\GAL4ppk.PG promotes F-actin structure formation in the distal dendrites of dorsal cluster neurons.

      (Lee et al., 2011)

      Overexpression of Rac1Scer\UAS.cLa in many neuron types, including the da neurons, under the control of Scer\GAL4477, leads to hyperbranching of dendrites.

      (Ye et al., 2011)

      Expression of Rac1Scer\UAS.cLa under the control of the neuronal drivers Scer\GAL4BG380, Scer\GAL4OK6 or Scer\GAL4elav-C155 leads to neuromuscular junction overgrowth during larval development, resulting in an increase in the number of synaptic boutons, branches and the appearance of abnormal synaptic protrusions. No overgrowth is seen when Rac1Scer\UAS.cLa is expressed in muscle under the control of the Scer\GAL4Mhc.PW driver.

      Expression of Rac1Scer\UAS.cLa under the control of either Scer\GAL4BG380 or Scer\GAL4OK6 produces increased mean evoked excitatory junctional potentials (EJPs) and quantal content in third instar larvae compared to controls. The size of miniature excitatory junctional potentials (mEJPs) does not differ from controls.

      (Ball et al., 2010)

      Expression of Rac1Scer\UAS.cLa under the control of Scer\GAL4DB331 does not alter the overall dendritic architecture in the lobular plate tangential cells; neither the position not branching patterns of primary and secondary order dendrites are affected. However, there is an increase in spine density and a change in spine morphology (the spines appear shorter and less well defined) compared to controls.

      (Leiss et al., 2009)

      Expression of Rac1Scer\UAS.cLa under the control of Scer\GAL4221 in class I neurons promotes de novo branch initiation reminiscent of, but not identical to, the filopodia of class III neurons.

      (Jinushi-Nakao et al., 2007)

      Expression of Rac1Scer\UAS.cLa under the control of cer\GAL4ato.3.6 inhibits the extension of axons from dorsal cluster neurons (DCNs), with an average of only 7.8 axons crossing toward the medulla instead of the wild-type average of 11.7. Expression of this transgene also results in a decreased number of DCN axons crossing the optic chiasm at 20%-30% pupal development.

      (Srahna et al., 2006)

      Expression of Rac1Scer\UAS.cLa, under the regulation of Scer\GAL4109(2)80, dramatic increases in larval neurons dendritic branching are observed. This is due to the formation of filopodia on normally non-filopodia bearing neurons (non-class III dendritic arborisation neurons), without altering the filopodia density. This branching phenotype becomes apparent in newly-hatched first instar larvae. Rac1Scer\UAS.cLa expression, under the regulation of Scer\GAL4109(2)80, like CaMKIIT287D.Scer\UAS expression, increases actin turnover, although the effects on morphological stability are significantly different.

      (Andersen et al., 2005)

      Expression of Rac1Scer\UAS.cLa under the control of Scer\GAL4GMR.PF results in about 90% pupal lethality. Escaper adults have strongly reduced eyes with no organised structure.

      (Avet-Rochex et al., 2005)

      When Rac1Scer\UAS.cLa is driven by Scer\GAL4GMR.PF a rough eye phenotype is seen.

      (Hu et al., 2005)

      When Rac1Scer\UAS.cLa is driven by Scer\GAL4477, an overbranching phenotype is seen in class IV neurons.

      (Emoto et al., 2004)

      Expression of Rac1Scer\UAS.cLa under the control of Scer\GAL4slbo.2.6 at 25oC does not alter the migration of border follicle cells that occurs during oogenesis.

      (Geisbrecht and Montell, 2004)

      When expression is driven by Scer\GAL4He.PZ, hemocyte proliferation and lamellocyte numbers are increased, and crystal cells are unaffected. Melanotic masses form. Similar, though milder, effects are seen when expression is driven by Scer\GAL4Hml.PG.

      (Zettervall et al., 2004)

      When driven by Scer\GAL4sca-109-68 bristle defects occur. When driven by Scer\GAL4109-69 eyes are roughened. Lethality, when driven by Scer\GAL4109-79 acts in the embryonic-larval stages.

      (Younger, 2001.2)

      Targeted expression of Rac1Scer\UAS.cLa to the GF under the control of Scer\GAL4A307 results in over 95% of GF axons showing morphological aberrations whereas less than 15% of controls show defects. In the majority of specimens the axons grow out of the brain and into the second thoracic neuromere (T2) but fail to make the terminal bend that is characteristic of the gf presynatpic terminal. In addition the tips of the axons appear swollen and contain vesicle-like structures. Nine percent of axons fail to exit the brain and often cross the midline and project into the contralateral half of the brain.

      Over 90% of GFs in Scer\GAL4A307; Rac1Scer\UAS.cLa adult brains exhibit abnormalities. Although neurite outgrowth and branching appears normal, and the three major dendritic domains are always discernible, typically dendrite are thickened and display inappropriate minor branches. Swollen dendrites containing large vesicle-like structures are also observed.

      When Scer\GAL4A307; Rac1Scer\UAS.cLa flies are tested physiologically, they all exhibit a TTM response latency that is more than twice that of control flies. The fidelity of transmission for repetitive stimuli is also reduced and in most cases only a single response to the first stimulus occurs.

      Approximately 30% of Scer\GAL4c17; Rac1Scer\UAS.cLa GF axons exhibit a bendless phenotype with swollen terminals and no lateral bends while the remaining axons appear wild-type.

      Approximately 81% of Scer\GAL4A307; Rac1Scer\UAS.cLa flies exhibit a bendless GF phenotype, while 64% of Scer\GAL4A307; Rac1Scer\UAS.cLa GFs are bendless. The remainder exhibit wild-type bends or large abnormally shaped processes in the target region.

      (Allen et al., 2000)

      Expression is driven by Scer\GAL4en-e16E causes wing vein thickening, extra sensory organs and wing enlargement.

      (Sotillos and Campuzano, 2000)

      Scer\GAL4elav-C155-mediated expression does not affect the normal anatomy of the CNS pathways. Scer\GAL4elav-C155-mediated expression of Rac1L89.Scer\UAS slightly disrupts CNS axon pathway, gaps between segments suggest failure in axon extension. Coexpression of Rac1Scer\UAS.cLa under the same Scer\GAL4 driver causes a massive failure in axon extension, dramatically enhancing the effect of Rac1L89.Scer\UAS. Axons that do extend follow abnormal trajectories.

      (Kaufmann et al., 1998)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhanced by
      NOT Enhanced by
      Suppressed by
      NOT suppressed by
      Enhancer of
      NOT Enhancer of
      Statement
      Reference

      Scer\GAL460/Rac1UAS.cLa is a non-enhancer of abnormal neuroanatomy | heat sensitive phenotype of Nl1N-ts1

      (Song and Giniger, 2011)
      Suppressor of
      NOT Suppressor of
      Other
      Phenotype Manifest In
      Enhanced by
      NOT Enhanced by
      Statement
      Reference

      Rac1UAS.cLa, Scer\GAL4ato.3.6 has axon & dorsal cluster neuron phenotype, non-enhanceable by btlDN.UAS, Scer\GAL4ato.3.6

      (Srahna et al., 2006)
      Suppressed by
      Statement
      Reference

      Rac1UAS.cLa, Scer\GAL4Tub.PU has interommatidial cell | somatic clone phenotype, suppressible | partially by SCARΔ37/SCARΔ37

      (Malin et al., 2024)

      Rac1UAS.cLa, Scer\GAL4Tub.PU has ommatidium | somatic clone phenotype, suppressible | partially by SCARΔ37/SCARΔ37

      (Malin et al., 2024)

      Rac1UAS.cLa, Scer\GAL4Tub.PU has interommatidial cell | somatic clone phenotype, suppressible | partially by Pten3/Pten3

      (Malin et al., 2024)

      Rac1UAS.cLa, Scer\GAL4Tub.PU has ommatidium | somatic clone phenotype, suppressible | partially by Pten3/Pten3

      (Malin et al., 2024)

      Rac1UAS.cLa, Scer\GAL4Cg.PU has hemocyte phenotype, suppressible by Tcs5RNAi.UAS.462, Scer\GAL4Cg.PU

      (Molina et al., 2022)

      Rac1UAS.cLa, Scer\GAL4RapGAP1-OK6 has embryonic/larval neuromuscular junction | third instar larval stage phenotype, suppressible | partially by trioS137203/trio[+]

      (Ball et al., 2010)

      Rac1UAS.cLa, Scer\GAL4RapGAP1-OK6 has NMJ bouton | third instar larval stage phenotype, suppressible | partially by trioS137203/trio[+]

      (Ball et al., 2010)

      Rac1UAS.cLa, Scer\GAL4RapGAP1-OK6 has embryonic/larval neuromuscular junction | third instar larval stage phenotype, suppressible by trioS137203

      (Ball et al., 2010)

      Rac1UAS.cLa, Scer\GAL4RapGAP1-OK6 has NMJ bouton | third instar larval stage phenotype, suppressible by trioS137203

      (Ball et al., 2010)

      Rac1UAS.cLa, Scer\GAL4GMR.PF has eye phenotype, suppressible | partially by Paer\ExoSGAP.UAS, Scer\GAL4GMR.PF

      (Avet-Rochex et al., 2005)

      Rac1UAS.cLa, Scer\GAL4GMR.PF has ommatidium phenotype, suppressible by RhoGAP93BUAS.cHa, Scer\GAL4GMR.PF

      (Hu et al., 2005)

      Rac1UAS.cLa, Scer\GAL4GMR.PF has photoreceptor neuron phenotype, suppressible by RhoGAP93BUAS.cHa, Scer\GAL4GMR.PF

      (Hu et al., 2005)

      Rac1UAS.cLa, Scer\GAL4477 has multidendritic neuron & dendrite phenotype, suppressible | partially by trcUAS.Tag:FLAG, Scer\GAL4477

      (Emoto et al., 2004)

      Rac1UAS.cLa, Scer\GAL4477 has multidendritic neuron & dendrite | supernumerary phenotype, suppressible | partially by trcUAS.Tag:FLAG, Scer\GAL4477

      (Emoto et al., 2004)
      NOT suppressed by
      Enhancer of
      NOT Enhancer of
      Statement
      Reference

      Scer\GAL460/Rac1UAS.cLa is a non-enhancer of larval intersegmental nerve | heat sensitive phenotype of Nl1N-ts1

      (Song and Giniger, 2011)

      Scer\GAL460/Rac1UAS.cLa is a non-enhancer of larval intersegmental nerve branch ISNb of A1-7 | heat sensitive phenotype of Nl1N-ts1

      (Song and Giniger, 2011)
      Suppressor of
      Statement
      Reference

      Rac1UAS.cLa, Scer\GAL4Cg.PU is a suppressor | partially of hemocyte phenotype of Scer\GAL4Cg.PU, Tcs5RNAi.UAS.462

      (Molina et al., 2022)

      Scer\GAL4109(2)80/Rac1UAS.cLa is a suppressor | partially of dendrite | somatic clone | third instar larval stage phenotype of fs(1)h1112

      (Bagley et al., 2014)

      Scer\GAL4109(2)80/Rac1UAS.cLa is a suppressor | partially of larval multidendritic class III neuron | somatic clone | third instar larval stage phenotype of fs(1)h1112

      (Bagley et al., 2014)

      Rac1UAS.cLa, Scer\GAL4en.PU is a suppressor of embryo | dorsal closure stage phenotype of PtenUAS.cGa, Scer\GAL4en.PU

      (Pickering et al., 2013)

      Rac1UAS.cLa, Scer\GAL4en.PU is a suppressor of embryonic leading edge cell phenotype of PtenUAS.cGa, Scer\GAL4en.PU

      (Pickering et al., 2013)

      Rac1UAS.cLa, Scer\GAL4ppk.PG is a suppressor | partially of larval multidendritic class IV neuron | larval stage phenotype of Hsap\ATXN3tr.Q78.UAS.Tag:HA, Scer\GAL4ppk.PG

      (Lee et al., 2011)

      Rac1UAS.cLa, Scer\GAL4109(2)80 is a suppressor | partially of lobula columnar neuron LC14 | larval stage phenotype of Hsap\ATXN3tr.Q78.UAS.Tag:HA, Scer\GAL4109(2)80

      (Lee et al., 2011)

      Rac1UAS.cLa, Scer\GAL4ppk.PG is a suppressor | partially of filamentous actin phenotype of Hsap\ATXN3tr.Q78.UAS.Tag:HA, Scer\GAL4ppk.PG

      (Lee et al., 2011)

      Rac1UAS.cLa is a suppressor of mesoderm phenotype of Scer\GAL4twi.2PE, pblΔBRCT.UAS.Tag:MYC, pbl3

      (van Impel et al., 2009)

      Rac1UAS.cLa, Scer\GAL4ato.3.6 is a suppressor of axon & dorsal cluster neuron & adult brain phenotype of Scer\GAL4ato.3.6, btlDN.UAS

      (Srahna et al., 2006)

      Rac1UAS.cLa, Scer\GAL4elav.PLu is a suppressor of larval VL3/4 motor neuron phenotype of PlexBUAS.cHa, Scer\GAL4elav.PLu

      (Hu et al., 2001)

      Rac1UAS.cLa, Scer\GAL4elav.PLu is a suppressor of larval intersegmental nerve phenotype of PlexBUAS.cHa, Scer\GAL4elav.PLu

      (Hu et al., 2001)

      Rac1UAS.cLa, Scer\GAL4en-e16E is a suppressor | partially of wing vein L4 phenotype of RafK497M.UAS, Scer\GAL4en-e16E

      (Sotillos and Campuzano, 2000)

      Rac1UAS.cLa, Scer\GAL4en-e16E is a suppressor | partially of wing vein L5 phenotype of RafK497M.UAS, Scer\GAL4en-e16E

      (Sotillos and Campuzano, 2000)

      Rac1UAS.cLa, Scer\GAL4en-e16E is a suppressor | partially of wing phenotype of RafK497M.UAS, Scer\GAL4en-e16E

      (Sotillos and Campuzano, 2000)
      NOT Suppressor of
      Statement
      Reference

      Scer\GAL4sns.PK/Rac1UAS.cLa is a non-suppressor of embryonic myoblast phenotype of mbcD11.2

      (Haralalka et al., 2011)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Expression of PuraGD12260 does not suppress the increase in length of the behavioral rhythm period seen when Rac1Scer\UAS.cLa is expressed under the control of Scer\GAL4P0.5.Pdf.

      (Petsakou et al., 2015)

      Expression of Rac1Scer\UAS.cLa driven by Scer\GAL4109(2)80 partially suppresses the reduction of dendritic spikes seen in class III da sensory neuron fs(1)h1112/fs(1)h1112 larval clones.

      Loss of fs(1)h1112/fs(1)h1112 in class I da neuron clones does not suppress the increase in dendrite branch points seen in larvae with Rac1Scer\UAS.cLa overexpression driven by Scer\GAL4109(2)80.

      (Bagley et al., 2014)

      Co-expression of Rac1Scer\UAS.cLa suppresses the decrease in zippering speed during dorsal closure seen in cells expressing PtenScer\UAS.cGa under the control of Scer\GAL4en.PU. The reduction in number of filopodia at the leading edge is also suppressed.

      (Pickering et al., 2013)

      Expression of Rac1Scer\UAS.cLa under the control of Scer\GAL4sns.PK does not rescue myoblast fusion in homozygous mbcD11.2 embryos.

      (Haralalka et al., 2011)

      The ISNb bypass phenotype of Nl1N-ts1 mutant embryos is not significantly modulated by expression of Rac1Scer\UAS.cLa under the control of Scer\GAL460.

      (Song and Giniger, 2011)

      A dar13010 mutant background does not block the dendrite branching activity found upon expression of Rac1Scer\UAS.cLa under the control of Scer\GAL4477.

      (Ye et al., 2011)

      One copy of trioS137203 partially suppresses the larval neuromuscular junction overgrowth and bouton number phenotypes seen when Rac1Scer\UAS.cLa is expressed in neurons under the control of Scer\GAL4OK6. Bouton number is reduced by more than 50%.

      One copy of sifES11 is unable to suppress the increase in larval neuromuscular junction bouton number seen when Rac1Scer\UAS.cLa is expressed in neurons under the control of Scer\GAL4OK6.

      Homozygous trioS137203 suppresses the larval neuromuscular junction overgrowth and increase in bouton number seen when Rac1Scer\UAS.cLa is expressed in neurons under the control of Scer\GAL4OK6.

      witA12/witHA3 suppresses the increase in evoked excitatory junctional potential (EJPs) and quantal content seen when Rac1Scer\UAS.cLa is expressed in motor neurons under the control of Scer\GAL4BG380, with third instar larvae instead displaying the reduction in neurotransmitter release phenotype seen in witA12/witHA3 mutants.

      trioS137203/trio6A suppresses the increase in evoked excitatory junctional potential (EJPs) and quantal content seen when Rac1Scer\UAS.cLa is expressed in neurons under the control of Scer\GAL4OK6.

      (Ball et al., 2010)

      Co-expression of Rac1Scer\UAS.cLa enhances the partial rescue of pbl3 mesodermal migration defects seen when pblΔBRCT.Scer\UAS.T:Hsap\MYC is expressed under the control of Scer\GAL4twi.2PE.

      Co-expression of Rac1Scer\UAS.cLa strongly enhances the eye phenotype seen when pblDH-PH.Scer\UAS.T:Ivir\HA1 is expressed under the control of Scer\GAL4GMR.PU. Most flies die as pharate adults but escapers fail to develop any eye structures.

      (van Impel et al., 2009)

      Ectopic expression of Rac1Scer\UAS.cLa along with ctScer\UAS.cPa in class I neurons under the control of Scer\GAL4221 leads to the formation of an arbor with many filpodia/spikes, which shows a significant enhancement of branching as compared to either Rac1Scer\UAS.cLa or ctScer\UAS.cPa expression alone.

      Ectopic expression of Rac1Scer\UAS.cLa along with knScer\UAS.cMa in class I neurons under the control of Scer\GAL4221 promotes the outgrowth of bona fide dendrites from the thorn-shaped projections initiated by Rac1Scer\UAS.cLa expression. In addition, coexpression of knScer\UAS.cMa causes a small, significant reduction in termini number.

      (Jinushi-Nakao et al., 2007)

      Coexpression of Rac1Scer\UAS.cLa and btlDN.Scer\UAS, under the control of Scer\GAL4ato.3.6, results in the dominance of the Rac1Scer\UAS.cLa phenotype, with a reduction in the number of dorsal cluster neuron axons crossing toward the medulla. This indicates that the Rac1N17.Scer\UAS phenotype is dominant.

      (Srahna et al., 2006)

      the addition of Rac1Scer\UAS.cLa to plexBScer\UAS.cHa, Scer\GAL4elav.PLu flies suppresses the Intersegmental nerve and RP3 nerve phenotypes.

      (Hu et al., 2001)
      Xenogenetic Interactions
      Statement
      Reference

      Expression of Rac1Scer\UAS.cLa partially suppresses the dendrite defects seen in class IV da neurons expressing Hsap\ATXN3tr.Q78.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4ppk.PG. The reduction in F-actin structures in the distal dendrites of dorsal cluster neurons when Hsap\ATXN3tr.Q78.Scer\UAS.T:Ivir\HA1 is expressed under the control of Scer\GAL4109(2)80 is also partially rescued.

      (Lee et al., 2011)

      The viability of flies expressing Rac1Scer\UAS.cLa under the control of Scer\GAL4GMR.PF is enhanced to 50% by co-expression of Zzzz\ExoSGAP.Scer\UAS. In addition, the eye morphology of the surviving adults is restored towards wild type.

      (Avet-Rochex et al., 2005)
      Complementation and Rescue Data
      Partially rescues

      Scer\GAL4slbo.2.6/Rac1UAS.cLa partially rescues Rac1N17.UAS

      (Geisbrecht and Montell, 2004)

      Scer\GAL4elav-C155/Rac1UAS.cLa partially rescues Rac1N17.UAS

      (Kaufmann et al., 1998)
      Comments

      Expression of Rac1Scer\UAS.cLa under the control of Scer\GAL4sns.PK strongly rescues the myoblast fusion defects of Rac1J11 Rac2Δ double mutant embryos, resulting in a near normal somatic muscle pattern. In contrast, expression of Rac1Scer\UAS.cLa under the control of Scer\GAL4kirre-rP298 rescues the myoblast fusion defects of Rac1J11 Rac2Δ double mutant embryos much less efficiently.

      (Haralalka et al., 2011)

      The border follicle cell migration defect seen in egg chambers expressing Rac1N17.Scer\UAS under the control of Scer\GAL4slbo.2.6 is partially suppressed by coexpression of Rac1Scer\UAS.cLa.

      (Geisbrecht and Montell, 2004)

      Restores CNS pathways to near normal morphology, some abnormalities can be seen in occasional segments.

      (Kaufmann et al., 1998)
      Images (0)
      Mutant
      Wild-type
      Stocks (3)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Rac1Scer\UAS.cLa
      Rac1UAS.cLa
      Name Synonyms
      Saccharomyces cerevisiae UAS construct of Luo
      (Luo et al., 1994)
      Secondary FlyBase IDs
        References (46)