|Name||Saccharomyces cerevisiae UAS construct a of Castelli-Gair||FlyBase ID||FBal0039101|
|Feature type||allele||Associated gene||Dmel\Ubx|
|Mutagen||in vitro construct - regulatory fusion|
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Controlled Vocabulary Terms
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|Nature of the Allele|
|Mutations Mapped to the Genome|
|Associated Sequence Data|
|Nature of the lesion|
|Carried in construct|
(Apitz et al., 2005, Gebelein et al., 2004, Bello et al., 2003, Brodu et al., 2002, Grenier and Carroll, 2000, Li et al., 1999, Monier et al., 2005, Galant and Carroll, 2002, Ronshaugen et al., 2002, Jiao et al., 2001, Yao et al., 1999, Prasad et al., 2003, Ryan et al., 2005, de Navas et al., 2006, Galant et al., 2002, Lovato et al., 2002, Janody et al., 2004, Ponzielli et al., 2002, Castelli-Gair et al., 1994, Shashidhara et al., 1999, Li and McGinnis, 1999, Adachi-Yamada et al., 2005, Casares et al., 1997, Sprecher et al., 2004, Mandal et al., 2004, Bajpai et al., 2004, Perrin et al., 2004, Prokop et al., 1998, Greig and Akam, 1995, Pallavi and Shashidhara, 2003, Pallavi et al., 2006, Stultz et al., 2006, Mohit et al., 2006, de Navas et al., 2006, LaBeau et al., 2009, Makhijani et al., 2007, Bischoff et al., 2009, de Navas et al., 2011, Merabet et al., 2007, Ahn et al., 2011, Tripura et al., 2011, Percival-Smith et al., 2005)
|Phenotype Manifest In|
embryonic central nervous system & thoracic segment
peripodial epithelium & dorsal mesothoracic disc, with Scer\GAL4vg.PM
Expression of Ubx[Scer\UAS.cCa] under the control of Scer\GAL4[sd.PU] or Scer\GAL4[Bx-MS1096] results in a transformation of wing to haltere.
Ectopic expression of Ubx[Scer\UAS.cCa] driven by Scer\GAL4[vg.PM] leads to small, crumpled wings with small necrotic cells.
Embryos ectopically expressing Scer\GAL4[how-24B] driven Ubx[Scer\UAS.cCa] throughout the mesoderm show an increased number of alary muscle pairs, from the seven normally observed in wild-type, to approximately ten. The supernumerary muscles are usually paired, and in many cases the most anterior pair of muscles is angled acutely to ensure contact with the dorsal vessel.
Expression of UbxScer\UAS.cCa under the control of Scer\GAL4dpp.blk1 results in flies with smaller wings than normal.
When UbxScer\UAS.cCa is driven by Scer\GAL4sd-SG29.1 abnormal wing vein patterns and incisions in the wing margin are seen.
UbxScer\UAS.cCa expression in all muscle cells at the onset of metamorphosis under the control of Scer\GAL4how-24B (and Scer\GAL80ts.αTub84B, placed at the restrictive temperature of 29oC at the onset of metamorphosis) results in tin-expressing myocytes in segments A1-A4 adopting the characteristics of adult segment A5 tin-expressing myocytes, including longitudinal orientation of the muscle fibers. UbxScer\UAS.cCa expression in cardiac myocytes under the control of Scer\GAL4NP5169 (and Scer\GAL80ts.αTub84B, blocking Scer\GAL4NP5169, placed at the restrictive temperature of 29oC at the onset of metamorphosis) results in tin-expressing myocytes in segments A1-A4 adopting the characteristics of adult segment A5 tin-expressing myocytes, including longitudinal orientation of the muscle fibers. UbxScer\UAS.cCa expression in all muscle cells at the onset of metamorphosis under the control of Scer\GAL4how-24B (and Scer\GAL80ts.αTub84B, placed at the restrictive temperature of 29oC at the onset of metamorphosis) does not affect the differentiation of adult ostiae that develop from A1-A5 svp-expressing cells.
Expression of Ubx[Scer\UAS.cCa] under the control of Scer\GAL4[dpp.blk1] results in transformation of the arista to tarsus.
In stage 16 UbxScer\UAS.cCa; Scer\GAL4how-24B embryos, the number of cells in the dorsal vessel is increased from 104 to 116 cells compared to wild-type.
When clones expressing UbxScer\UAS.cCa (driven by Scer\GAL4αTub84B.PL) in the posterior of eye disc, photoreceptor differentiation is blocked .
In stage-15 mutant embryos in which UbxScer\UAS.cCa is overexpressed under the control of Scer\GAL4Mef2.PR, lymph-gland progenitors are replaced by pericardial nephrocytes.
In mutant embryos expressing UbxScer\UAS.cCa driven by both Scer\GAL4twi.PG and Scer\GAL4how-24B ectopic cardioblasts are seen and the lymph glands are systematically eliminated and replaced by major pericardial cells. The anterior aorta is also transformed into a posterior aorta and heart tissue.
Expression of UbxScer\UAS.cCa under the control of Scer\GAL4sca-537.4 results in a mutant phenotype in the embryonic tritocerebrum. The phenotype has a penetrance of more than 95%.
Postembryonic neuroblast (pNB) clones in the larval thorax expressing UbxScer\UAS.cCa under the control of Scer\GAL4αTub84B.PL are reduced in size compared to wild-type clones and the clones show loss of the pNB in at least 82% of cases.
The number of peripodial membrane cells in the wing discs of UbxScer\UAS.cCa; Scer\GAL4vg.PM late 3rd instar larvae is decreased to less than 250 (from 400-450 in wild-type, wheras with UbxScer\UAS.cCa; Scer\GAL4426 it is increased to >550.
Expression of UbxScer\UAS.cCa under the control of Scer\GAL4en-e16E does not result in the formation of ectopic oenocytes in the T1-T3 segments.
Expression of UbxScer\UAS.cCa under the control of Scer\GAL4arm.PS in embryos results in the transformation of segment T3 into A1. The penetrance of this phenotype is 100%.
Expression of UbxScer\UAS.cCa under the control of Scer\GAL4arm.PS in embryos results in the transformation of the identity of third thoracic segment to that of abdominal segment A1.
Expression of UbxScer\UAS.cCa under the control of Scer\GAL4how-24B in embryos does not inhibit heart cell specification in segments A5-A8. There is often an increase in cell size in the dorsal vessel at the A4/A5 boundary, consistent with the formation of an ectopic ostium within the aorta.
Cardioblasts do not have normal morphology in embryos expressing UbxScer\UAS.cCa under the control of both Scer\GAL4twi.PG and Scer\GAL4how-24B and the ostiae are not functional.
94% of flies expressing UbxScer\UAS.cCa under the control of Scer\GAL4ey.PH are wild type. Some animals are seen in which most of the head and one or both eyes of reduced size are present.
Overexpression of UbxScer\UAS.cCa driven by Scer\GAL4dpp.blk1 causes transformation of the antenna towards a thoracic leg. Overexpression of UbxScer\UAS.cCa driven by Scer\GAL4Bx-MS1096 causes transformation of the wing towards a haltere in females. Males do not survive larval stages. The phenotype is stronger on the dorsal surface of the wing. Overexpression of UbxScer\UAS.cCa in the ectoderm, driven by Scer\GAL4arm.PS, transforms the denticle belts of thoracic segments towards A1 identity. The development of Keilin's organs and ventral pits are repressed.
When ectopically expressed by a strong Scer\GAL4arm.PS (arm-GAL4), UbxScer\UAS.cCa transforms head and all three thoracic segments into morphological replicas of A1 i.e. generating A1-like denticle belts in these more anterior segments. T1 develops without the characteristic patch of beard denticles. When ectopically expressed by a weak Scer\GAL4arm.PS (arm-GAL4r), UbxScer\UAS.cCa shows no abnormalities at all.
The overall shape of the central nervous system is not perturbed in embryos expressing UbxScer\UAS.cCa under the control of Scer\GAL4Mz1407. The pattern of BrdU incorporation in the central nervous system is abnormal in stage 16/17 embryos; the lateral replicating clusters of the thoracic neuromeres are abolished, and ectopic sites of BrdU incorporation are seen in the ventral region of the thoracic neuromeres.
Scer\GAL4twi.PG induced expression causes ectopic gonadal mesoderm formation in anterior segments of the embryo. Df(3R)Ubx109/Df(3R)P9 embryos lack gonads, ectopic expression of Ubx restores the formation of an encapsulated gonad.
Keilin's organs are suppressed in the UbxScer\UAS.cCa, Scer\GAL4h-1J3 or UbxScer\UAS.cCa, Scer\GAL4Kr.PM combination. With Scer\GAL469B, posterior spiracles are reduced to half their wild type size, in most embryos a small A9 denticle belt is formed anterior to the anal plates and the anal tuft is often reduced. Similar but weaker effects on the posterior spiracles are seen with Scer\GAL4h-1J3.
Scer\GAL4dpp.blk1, UbxScer\UAS.cCa has visible phenotype, suppressible by hthScer\UAS.cPa/Scer\GAL4dpp.blk1
|Phenotype Manifest In|
Scer\GAL4dpp.blk1, UbxScer\UAS.cCa has antenna phenotype, suppressible by hthScer\UAS.cPa/Scer\GAL4dpp.blk1
Scer\GAL4dpp.blk1, UbxScer\UAS.cCa has arista phenotype, suppressible by pbScer\UAS.T:Avic\GFP-EGFP/Scer\GAL4dpp.blk1
Scer\GAL4dpp.blk1, UbxScer\UAS.cCa has pretarsus | ectopic phenotype, suppressible by pbScer\UAS.T:Avic\GFP-EGFP/Scer\GAL4dpp.blk1
Co-expression of pb[Scer\UAS.T:Avic\GFP-EGFP] suppresses the arista-to-tarsus transformation seen in flies expressing Ubx[Scer\UAS.cCa] under the control of Scer\GAL4[dpp.blk1].
The defect in the embryonic tritocerebrum caused by expression of UbxScer\UAS.cCa under the control of Scer\GAL4sca-537.4 is completely suppressed if the embryos are also coexpressing both exdScer\UAS.NLS.T:Zzzz\FLAG and hthScer\UAS.cPa.
Co-expression of hthScer\UAS.cPa partially suppresses the antenna to leg transformation phenotypes produced by ectopic expression of UbxScer\UAS.cCa driven by Scer\GAL4dpp.blk1. The antennae produced are aristaless and occasionally duplicated, similar to the hthScer\UAS.cPa phenotype.
The segmentation phenotype of Df(3R)Ubx109 is modified in the UbxScer\UAS.cCa, Scer\GAL4h-1J3 combination to transform alternate segments to resemble a wild type A1. This transformation is complete in the UbxScer\UAS.cCa, Scer\GAL469B combination. The sensory organ phenotype of Df(3R)Ubx109 is modified in the UbxScer\UAS.cCa, Scer\GAL4h-1J3 or UbxScer\UAS.cCa, Scer\GAL4Kr.PM combination to prevent the ventral migration of the cells that would form the kolbchen, though later differentiation produces misplaced kolbchen rather than dorsal hairs. The sensory organ phenotype of Df(3R)Ubx109 is modified in the UbxScer\UAS.cCa, Scer\GAL469B combination to affect the migration and differentiation of the kolbchen.
|Complementation & Rescue Data|
The partial transformation of haltere into wing which is seen in Ubx[LDN]/Ubx[abx-1] Ubx[bx-3] Ubx[pbx-1] flies is strongly suppressed by expression of Ubx[Scer\UAS.cCa] under the control of Scer\GAL4[Ubx-LDN].
|Stocks ( 2 )|
|Notes on Origin|
|External Crossreferences & Linkouts|
|Synonyms & Secondary IDs ( 3 )|
Saccharomyces cerevisiae UAS construct a of Castelli-Gair
|Secondary FlyBase IDs|
|References ( 42 )|
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|Recent research papers ( 3 )|