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General Information
Symbol
Dmel\UbxUAS.cCa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Castelli-Gair
FlyBase ID
FBal0039101
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-Ubx, UAS-Ubx Ia, UAS-UbxIa.C, UAS-UbxIa, UAS-Ubx.Ia.C
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference
UAS regulatory sequences drive expression of a cDNA that encodes the Ubx 1a variant plus 229bp of 5'UTR sequence and 127bp of 3'UTR sequence..
Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
abdominal segment 1 & puparium & myoblast, with Scer\GAL4how-24B
abdominal segment 1 & puparium & myoblast, with Scer\GAL4NP5169
abdominal segment 1 & puparium & myofibril, with Scer\GAL4how-24B
abdominal segment 1 & puparium & myofibril, with Scer\GAL4NP5169
abdominal segment 2 & puparium & myoblast, with Scer\GAL4how-24B
abdominal segment 2 & puparium & myoblast, with Scer\GAL4NP5169
abdominal segment 2 & puparium & myofibril, with Scer\GAL4how-24B
abdominal segment 2 & puparium & myofibril, with Scer\GAL4NP5169
abdominal segment 3 & puparium & myoblast, with Scer\GAL4how-24B
abdominal segment 3 & puparium & myoblast, with Scer\GAL4NP5169
abdominal segment 3 & puparium & myofibril, with Scer\GAL4how-24B
abdominal segment 3 & puparium & myofibril, with Scer\GAL4NP5169
abdominal segment 4 & puparium & myoblast, with Scer\GAL4how-24B
abdominal segment 4 & puparium & myoblast, with Scer\GAL4NP5169
abdominal segment 4 & puparium & myofibril, with Scer\GAL4how-24B
abdominal segment 4 & puparium & myofibril, with Scer\GAL4NP5169
abdominal segment 5 & puparium & myoblast | ectopic, with Scer\GAL4how-24B
abdominal segment 5 & puparium & myoblast | ectopic, with Scer\GAL4NP5169
denticle belt & abdominal segment 9, with Scer\GAL469B
peripodial epithelium & dorsal mesothoracic disc, with Scer\GAL4426
peripodial epithelium & dorsal mesothoracic disc, with Scer\GAL4vg.PM
Detailed Description
Statement
Reference
The expression of UbxUAS.cCa under the control of Scer\GAL4pros.PMG induces a significant decrease in the mitotic index of neuroblast daughter cells in the embryonic ventral nerve cord T2 and T3 segments, but not in segment A1, despite of no significant changes in the mitotic index of neuroblasts in the same segments, as compared to controls.
Expression of UbxScer\UAS.cCa under the control of Scer\GAL4GMR24B02 (and thus transforming the 12A ventral nerve cord interneurons in segment T1 toward the T2 fate) leads to variable splitting and branching in T1 12A neurons at rates consistent with those typically seen in the T2 neuromere. However, within the animals ectopically expressing UbxScer\UAS.cCa in all 12A hemilineages, the proportions of neuronal wiring variants (neurite bundle split/unsplit, ectopic branches) differs between T1 and T2 segment neurons in the pupal brain.
Expression of UbxScer\UAS.cCa under the control of Scer\GAL4rn.PU suppresses wing formation.
Expression of UbxScer\UAS.cCa under the control of Scer\GAL4sd.PU or Scer\GAL4Bx-MS1096 results in a transformation of wing to haltere.
Ectopic expression of UbxScer\UAS.cCa driven by Scer\GAL4vg.PM leads to small, crumpled wings with small necrotic cells.
Embryos ectopically expressing Scer\GAL4how-24B driven UbxScer\UAS.cCa throughout the mesoderm show an increased number of alary muscle pairs, from the seven normally observed in wild-type, to approximately ten. The supernumerary muscles are usually paired, and in many cases the most anterior pair of muscles is angled acutely to ensure contact with the dorsal vessel.
Expression of UbxScer\UAS.cCa under the control of Scer\GAL4dpp.blk1 results in flies with smaller wings than normal.
When UbxScer\UAS.cCa is driven by Scer\GAL4sd-SG29.1 abnormal wing vein patterns and incisions in the wing margin are seen.
UbxScer\UAS.cCa expression in all muscle cells at the onset of metamorphosis under the control of Scer\GAL4how-24B (and Scer\GAL80ts.αTub84B, placed at the restrictive temperature of 29oC at the onset of metamorphosis) results in tin-expressing myocytes in segments A1-A4 adopting the characteristics of adult segment A5 tin-expressing myocytes, including longitudinal orientation of the muscle fibers. UbxScer\UAS.cCa expression in cardiac myocytes under the control of Scer\GAL4NP5169 (and Scer\GAL80ts.αTub84B, blocking Scer\GAL4NP5169, placed at the restrictive temperature of 29oC at the onset of metamorphosis) results in tin-expressing myocytes in segments A1-A4 adopting the characteristics of adult segment A5 tin-expressing myocytes, including longitudinal orientation of the muscle fibers. UbxScer\UAS.cCa expression in all muscle cells at the onset of metamorphosis under the control of Scer\GAL4how-24B (and Scer\GAL80ts.αTub84B, placed at the restrictive temperature of 29oC at the onset of metamorphosis) does not affect the differentiation of adult ostiae that develop from A1-A5 svp-expressing cells.
Expression of UbxScer\UAS.cCa under the control of Scer\GAL4dpp.blk1 results in transformation of the arista to tarsus.
In stage 16 UbxScer\UAS.cCa; Scer\GAL4how-24B embryos, the number of cells in the dorsal vessel is increased from 104 to 116 cells compared to wild-type.
When clones expressing UbxScer\UAS.cCa (driven by Scer\GAL4αTub84B.PL) in the posterior of eye disc, photoreceptor differentiation is blocked .
In stage-15 mutant embryos in which UbxScer\UAS.cCa is overexpressed under the control of Scer\GAL4Mef2.PR, lymph-gland progenitors are replaced by pericardial nephrocytes.
In mutant embryos expressing UbxScer\UAS.cCa driven by both Scer\GAL4twi.PG and Scer\GAL4how-24B ectopic cardioblasts are seen and the lymph glands are systematically eliminated and replaced by major pericardial cells. The anterior aorta is also transformed into a posterior aorta and heart tissue.
Expression of UbxScer\UAS.cCa under the control of Scer\GAL4sca-537.4 results in a mutant phenotype in the embryonic tritocerebrum. The phenotype has a penetrance of more than 95%.
Postembryonic neuroblast (pNB) clones in the larval thorax expressing UbxScer\UAS.cCa under the control of Scer\GAL4αTub84B.PL are reduced in size compared to wild-type clones and the clones show loss of the pNB in at least 82% of cases.
The number of peripodial membrane cells in the wing discs of UbxScer\UAS.cCa; Scer\GAL4vg.PM late 3rd instar larvae is decreased to less than 250 (from 400-450 in wild-type, wheras with UbxScer\UAS.cCa; Scer\GAL4426 it is increased to >550.
Expression of UbxScer\UAS.cCa under the control of Scer\GAL4en-e16E does not result in the formation of ectopic oenocytes in the T1-T3 segments.
Expression of UbxScer\UAS.cCa under the control of Scer\GAL4arm.PS in embryos results in the transformation of segment T3 into A1. The penetrance of this phenotype is 100%.
Expression of UbxScer\UAS.cCa under the control of Scer\GAL4arm.PS in embryos results in the transformation of the identity of third thoracic segment to that of abdominal segment A1.
Expression of UbxScer\UAS.cCa under the control of Scer\GAL4how-24B in embryos does not inhibit heart cell specification in segments A5-A8. There is often an increase in cell size in the dorsal vessel at the A4/A5 boundary, consistent with the formation of an ectopic ostium within the aorta.
Cardioblasts do not have normal morphology in embryos expressing UbxScer\UAS.cCa under the control of both Scer\GAL4twi.PG and Scer\GAL4how-24B and the ostiae are not functional.
94% of flies expressing UbxScer\UAS.cCa under the control of Scer\GAL4ey.PH are wild type. Some animals are seen in which most of the head and one or both eyes of reduced size are present.
Overexpression of UbxScer\UAS.cCa driven by Scer\GAL4dpp.blk1 causes transformation of the antenna towards a thoracic leg. Overexpression of UbxScer\UAS.cCa driven by Scer\GAL4Bx-MS1096 causes transformation of the wing towards a haltere in females. Males do not survive larval stages. The phenotype is stronger on the dorsal surface of the wing. Overexpression of UbxScer\UAS.cCa in the ectoderm, driven by Scer\GAL4arm.PS, transforms the denticle belts of thoracic segments towards A1 identity. The development of Keilin's organs and ventral pits are repressed.
When ectopically expressed by a strong Scer\GAL4arm.PS (arm-GAL4), UbxScer\UAS.cCa transforms head and all three thoracic segments into morphological replicas of A1 i.e. generating A1-like denticle belts in these more anterior segments. T1 develops without the characteristic patch of beard denticles. When ectopically expressed by a weak Scer\GAL4arm.PS (arm-GAL4r), UbxScer\UAS.cCa shows no abnormalities at all.
The overall shape of the central nervous system is not perturbed in embryos expressing UbxScer\UAS.cCa under the control of Scer\GAL4Mz1407. The pattern of BrdU incorporation in the central nervous system is abnormal in stage 16/17 embryos; the lateral replicating clusters of the thoracic neuromeres are abolished, and ectopic sites of BrdU incorporation are seen in the ventral region of the thoracic neuromeres.
Scer\GAL4twi.PG induced expression causes ectopic gonadal mesoderm formation in anterior segments of the embryo. Df(3R)Ubx109/Df(3R)P9 embryos lack gonads, ectopic expression of Ubx restores the formation of an encapsulated gonad.
Keilin's organs are suppressed in the UbxScer\UAS.cCa, Scer\GAL4h-1J3 or UbxScer\UAS.cCa, Scer\GAL4Kr.PM combination. With Scer\GAL469B, posterior spiracles are reduced to half their wild type size, in most embryos a small A9 denticle belt is formed anterior to the anal plates and the anal tuft is often reduced. Similar but weaker effects on the posterior spiracles are seen with Scer\GAL4h-1J3.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT Enhanced by
Suppressed by
NOT Enhancer of
Other
Phenotype Manifest In
NOT Enhanced by
Suppressed by
NOT Enhancer of
Other
Additional Comments
Genetic Interactions
Statement
Reference
The decreased mitotic index of neuroblast daughter cells in the embryonic ventral nerve cord T2 and T3 segments induced by the expression of UbxUAS.cCa under the control of Scer\GAL4pros.PMG is not enhanced by Df(3L)ED225 or by the additional double co-expression of abd-AUAS.Tag:FLAG and Abd-BUAS.Tag:V5. Df(3L)ED225 mutants also expressing UbxUAS.cCa under the control of Scer\GAL4pros.PMG show a significant decrease in the mitotic index of neuroblasts in the embryonic ventral nerve cord T2 and T3 segments, as compared to either single background condition. The triple co-expression of UbxUAS.cCa, abd-AUAS.Tag:FLAG and Abd-BUAS.Tag:V5 leads to a significant decrease in the number of NB3-5T lineage cells (Scer\GAL4GMR80G10-driving expression) and NB5-6T lineage cells (Scer\GAL4pros.PMG-driving expression) in the T1 and T3 segments, associated with both a severe decrease in the mitotic index of neuroblasts in the NB5-6T lineage, but not in the NB3-5T lineage, and a severe decrease in the mitotic index of the neuroblast daughter cells in the NB3-5T lineage, but not in the NB5-6T lineage, as compared to wild-type controls.
Addition of UbxScer\UAS.cCa to Dfd16;Scr4 double mutant embryos (under the control of Scer\GAL4salm-459.2) results in both the Mx and Lb segments forming tracheal tubes instead of migratory gland primordia.
Co-expression of pbScer\UAS.T:Avic\GFP-EGFP suppresses the arista-to-tarsus transformation seen in flies expressing UbxScer\UAS.cCa under the control of Scer\GAL4dpp.blk1.
The defect in the embryonic tritocerebrum caused by expression of UbxScer\UAS.cCa under the control of Scer\GAL4sca-537.4 is completely suppressed if the embryos are also coexpressing both exdScer\UAS.NLS.T:Zzzz\FLAG and hthScer\UAS.cPa.
Co-expression of hthScer\UAS.cPa partially suppresses the antenna to leg transformation phenotypes produced by ectopic expression of UbxScer\UAS.cCa driven by Scer\GAL4dpp.blk1. The antennae produced are aristaless and occasionally duplicated, similar to the hthScer\UAS.cPa phenotype.
The segmentation phenotype of Df(3R)Ubx109 is modified in the UbxScer\UAS.cCa, Scer\GAL4h-1J3 combination to transform alternate segments to resemble a wild type A1. This transformation is complete in the UbxScer\UAS.cCa, Scer\GAL469B combination. The sensory organ phenotype of Df(3R)Ubx109 is modified in the UbxScer\UAS.cCa, Scer\GAL4h-1J3 or UbxScer\UAS.cCa, Scer\GAL4Kr.PM combination to prevent the ventral migration of the cells that would form the kolbchen, though later differentiation produces misplaced kolbchen rather than dorsal hairs. The sensory organ phenotype of Df(3R)Ubx109 is modified in the UbxScer\UAS.cCa, Scer\GAL469B combination to affect the migration and differentiation of the kolbchen.
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
The partial transformation of haltere into wing which is seen in UbxLDN/Ubxabx-1 Ubxbx-3 Ubxpbx-1 flies is strongly suppressed by expression of UbxScer\UAS.cCa under the control of Scer\GAL4Ubx-LDN.
Expression of UbxScer\UAS.cCa rescues the phenotype of UbxLDN, Ubxbx-3, Ubxpbx-1, Ubxabx-1 flies.
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
UbxScer\UAS.cCa
UbxUAS.cCa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Castelli-Gair
Secondary FlyBase IDs
    References (49)