Allele Dmel\Ubx^Scer\UAS.cCa

General Information
Symbol	Dmel\Ubx^Scer\UAS.cCa	Species	D. melanogaster
Name	Saccharomyces cerevisiae UAS construct a of Castelli-Gair	FlyBase ID	FBal0039101
Feature type	allele	Associated gene	Dmel\Ubx

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02	Controlled Vocabulary Terms arista pretarsus
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Castelli-Gair et al., 1994, Prokop et al., 1998)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: The cDNA coding region for Ubx variant 1a cloned downstream of the Scer\GAL4 response element UAS. (Castelli-Gair et al., 1994)
Carried in construct	P{UAS-Ubx.Ia.C} (Apitz et al., 2005, Gebelein et al., 2004, Bello et al., 2003, Brodu et al., 2002, Grenier and Carroll, 2000, Li et al., 1999, Monier et al., 2005, Galant and Carroll, 2002, Ronshaugen et al., 2002, Jiao et al., 2001, Yao et al., 1999, Prasad et al., 2003, Ryan et al., 2005, de Navas et al., 2006, Galant et al., 2002, Lovato et al., 2002, Janody et al., 2004, Ponzielli et al., 2002, Castelli-Gair et al., 1994, Shashidhara et al., 1999, Li and McGinnis, 1999, Adachi-Yamada et al., 2005, Casares et al., 1997, Sprecher et al., 2004, Mandal et al., 2004, Bajpai et al., 2004, Perrin et al., 2004, Prokop et al., 1998, Greig and Akam, 1995, Pallavi and Shashidhara, 2003, Pallavi et al., 2006, Stultz et al., 2006, Mohit et al., 2006, de Navas et al., 2006, LaBeau et al., 2009, Makhijani et al., 2007, Bischoff et al., 2009, de Navas et al., 2011, Merabet et al., 2007, Ahn et al., 2011, Tripura et al., 2011, Percival-Smith et al., 2005)
Cytology
Phenotypic Data
Phenotypic Class
	lethal \| male, with Scer\GAL4^Bx-MS1096 (Grenier and Carroll, 2000) visible, with Scer\GAL4^Bx-MS1096 (de Navas et al., 2011) visible, with Scer\GAL4^dpp.blk1 (Yao et al., 1999, de Navas et al., 2006, Percival-Smith et al., 2005) visible, with Scer\GAL4^ey.PH (Jiao et al., 2001) visible, with Scer\GAL4^sd.PU (de Navas et al., 2011) visible, with Scer\GAL4^vg.PM (Bischoff et al., 2009)
Phenotype Manifest In
	abdominal 1 ventral denticle belt \| ectopic, with Scer\GAL4^arm.PS (Grenier and Carroll, 2000) abdominal 11 anal tuft, with Scer\GAL4^69B (Castelli-Gair et al., 1994) abdominal segment 1 & puparium & myoblast, with Scer\GAL4^how-24B (Monier et al., 2005) abdominal segment 1 & puparium & myoblast, with Scer\GAL4^NP5169 (Monier et al., 2005) abdominal segment 1 & puparium & myofibril, with Scer\GAL4^how-24B (Monier et al., 2005) abdominal segment 1 & puparium & myofibril, with Scer\GAL4^NP5169 (Monier et al., 2005) abdominal segment 1 \| ectopic, with Scer\GAL4^arm.PS (Li and McGinnis, 1999) abdominal segment 2 & puparium & myoblast, with Scer\GAL4^how-24B (Monier et al., 2005) abdominal segment 2 & puparium & myoblast, with Scer\GAL4^NP5169 (Monier et al., 2005) abdominal segment 2 & puparium & myofibril, with Scer\GAL4^how-24B (Monier et al., 2005) abdominal segment 2 & puparium & myofibril, with Scer\GAL4^NP5169 (Monier et al., 2005) abdominal segment 3 & puparium & myoblast, with Scer\GAL4^how-24B (Monier et al., 2005) abdominal segment 3 & puparium & myoblast, with Scer\GAL4^NP5169 (Monier et al., 2005) abdominal segment 3 & puparium & myofibril, with Scer\GAL4^how-24B (Monier et al., 2005) abdominal segment 3 & puparium & myofibril, with Scer\GAL4^NP5169 (Monier et al., 2005) abdominal segment 4 & puparium & myoblast, with Scer\GAL4^how-24B (Monier et al., 2005) abdominal segment 4 & puparium & myoblast, with Scer\GAL4^NP5169 (Monier et al., 2005) abdominal segment 4 & puparium & myofibril, with Scer\GAL4^how-24B (Monier et al., 2005) abdominal segment 4 & puparium & myofibril, with Scer\GAL4^NP5169 (Monier et al., 2005) abdominal segment 5 & puparium & myoblast \| ectopic, with Scer\GAL4^how-24B (Monier et al., 2005) abdominal segment 5 & puparium & myoblast \| ectopic, with Scer\GAL4^NP5169 (Monier et al., 2005) adult head, with Scer\GAL4^ey.PH (Jiao et al., 2001) antenna, with Scer\GAL4^dpp.blk1 (Grenier and Carroll, 2000, Yao et al., 1999) aorta primordium \| anterior, with Scer\GAL4^how-24B, Scer\GAL4^twi.PG (Perrin et al., 2004) aorta primordium \| ectopic \| posterior, with Scer\GAL4^how-24B, Scer\GAL4^twi.PG (Perrin et al., 2004) arista, with Scer\GAL4^dpp.blk1 (Percival-Smith et al., 2005) cardioblast, with Scer\GAL4^twi.PG (Ponzielli et al., 2002) denticle belt & abdominal segment 9, with Scer\GAL4^69B (Castelli-Gair et al., 1994) embryonic/larval dorsal vessel, with Scer\GAL4^how-24B (Ryan et al., 2005) embryonic/larval lymph gland, with Scer\GAL4^how-24B, Scer\GAL4^twi.PG (Perrin et al., 2004) embryonic/larval ostia, with Scer\GAL4^twi.PG (Ponzielli et al., 2002) embryonic/larval ostia \| ectopic, with Scer\GAL4^how-24B (Lovato et al., 2002) embryonic/larval pericardial cell \| ectopic, with Scer\GAL4^how-24B, Scer\GAL4^twi.PG (Perrin et al., 2004) embryonic/larval posterior spiracle, with Scer\GAL4^69B (Castelli-Gair et al., 1994) embryonic/larval posterior spiracle, with Scer\GAL4^h-1J3 (Castelli-Gair et al., 1994) embryonic abdominal segment 1 \| ectopic, with Scer\GAL4^arm.PS (Galant and Carroll, 2002, Galant et al., 2002) embryonic central nervous system & thoracic segment (Prokop et al., 1998) embryonic epidermis, with Scer\GAL4^arm.PS (Grenier and Carroll, 2000) embryonic head, with Scer\GAL4^arm.PS (Li and McGinnis, 1999) embryonic mesothoracic segment, with Scer\GAL4^arm.PS (Li and McGinnis, 1999) embryonic metathoracic segment, with Scer\GAL4^arm.PS (Galant and Carroll, 2002, Galant et al., 2002, Li and McGinnis, 1999) embryonic pericardial cell, with Scer\GAL4^Mef2.PR (Mandal et al., 2004) embryonic prothoracic segment, with Scer\GAL4^arm.PS (Li and McGinnis, 1999) embryonic tritocerebrum, with Scer\GAL4^sca-537.4 (Sprecher et al., 2004) embryonic visceral muscle \| supernumerary, with Scer\GAL4^how-24B (LaBeau et al., 2009) eye, with Scer\GAL4^ey.PH (Jiao et al., 2001) eye disc \| posterior \| somatic clone, with Scer\GAL4^αTub84B.PL (Janody et al., 2004) gonadal sheath proper primordium \| ectopic, with Scer\GAL4^twi.PG (Greig and Akam, 1995) haltere \| ectopic, with Scer\GAL4^Bx-MS1096 (Grenier and Carroll, 2000, de Navas et al., 2011) haltere \| ectopic, with Scer\GAL4^sd.PU (de Navas et al., 2011) Keilin's organ, with Scer\GAL4^arm.PS (Grenier and Carroll, 2000) Keilin's organ, with Scer\GAL4^h-1J3 (Castelli-Gair et al., 1994) Keilin's organ, with Scer\GAL4^Kr.PM (Castelli-Gair et al., 1994) Kolbchen, with Scer\GAL4^arm.PS (Grenier and Carroll, 2000) larval neuromere \| embryonic stage (Prokop et al., 1998) leg \| ectopic, with Scer\GAL4^dpp.blk1 (Grenier and Carroll, 2000) lymph gland primordium, with Scer\GAL4^Mef2.PR (Mandal et al., 2004) mesothoracic ventral denticle belt, with Scer\GAL4^arm.PS (Li and McGinnis, 1999) metathoracic ventral denticle belt, with Scer\GAL4^arm.PS (Li and McGinnis, 1999) peripodial epithelium & dorsal mesothoracic disc, with Scer\GAL4⁴²⁶ (Pallavi and Shashidhara, 2003) peripodial epithelium & dorsal mesothoracic disc, with Scer\GAL4^vg.PM (Pallavi and Shashidhara, 2003) photoreceptor cell \| somatic clone, with Scer\GAL4^αTub84B.PL (Janody et al., 2004) pretarsus \| ectopic, with Scer\GAL4^dpp.blk1 (Percival-Smith et al., 2005) prothoracic ventral denticle belt, with Scer\GAL4^arm.PS (Li and McGinnis, 1999) T1 beard \| embryonic stage, with Scer\GAL4^arm.PS (Li and McGinnis, 1999) thoracic neuroblast \| larval stage, with Scer\GAL4^αTub84B.PL (Bello et al., 2003) wing, with Scer\GAL4^Bx-MS1096 (Grenier and Carroll, 2000, de Navas et al., 2011) wing, with Scer\GAL4^dpp.blk1 (de Navas et al., 2006) wing, with Scer\GAL4^sd.PU (de Navas et al., 2011) wing blade, with Scer\GAL4^vg.PM (Bischoff et al., 2009) wing margin, with Scer\GAL4^sd-SG29.1 (Adachi-Yamada et al., 2005) wing vein, with Scer\GAL4^sd-SG29.1 (Adachi-Yamada et al., 2005)
Detailed Description
	Statement Reference Expression of Ubx[Scer\UAS.cCa] under the control of Scer\GAL4[sd.PU] or Scer\GAL4[Bx-MS1096] results in a transformation of wing to haltere. (de Navas et al., 2011) Ectopic expression of Ubx[Scer\UAS.cCa] driven by Scer\GAL4[vg.PM] leads to small, crumpled wings with small necrotic cells. (Bischoff et al., 2009) Embryos ectopically expressing Scer\GAL4[how-24B] driven Ubx[Scer\UAS.cCa] throughout the mesoderm show an increased number of alary muscle pairs, from the seven normally observed in wild-type, to approximately ten. The supernumerary muscles are usually paired, and in many cases the most anterior pair of muscles is angled acutely to ensure contact with the dorsal vessel. (LaBeau et al., 2009) Expression of Ubx^Scer\UAS.cCa under the control of Scer\GAL4^dpp.blk1 results in flies with smaller wings than normal. (de Navas et al., 2006) When Ubx^Scer\UAS.cCa is driven by Scer\GAL4^sd-SG29.1 abnormal wing vein patterns and incisions in the wing margin are seen. (Adachi-Yamada et al., 2005) Ubx^Scer\UAS.cCa expression in all muscle cells at the onset of metamorphosis under the control of Scer\GAL4^how-24B (and Scer\GAL80^ts.αTub84B, placed at the restrictive temperature of 29^oC at the onset of metamorphosis) results in tin-expressing myocytes in segments A1-A4 adopting the characteristics of adult segment A5 tin-expressing myocytes, including longitudinal orientation of the muscle fibers. Ubx^Scer\UAS.cCa expression in cardiac myocytes under the control of Scer\GAL4^NP5169 (and Scer\GAL80^ts.αTub84B, blocking Scer\GAL4^NP5169, placed at the restrictive temperature of 29^oC at the onset of metamorphosis) results in tin-expressing myocytes in segments A1-A4 adopting the characteristics of adult segment A5 tin-expressing myocytes, including longitudinal orientation of the muscle fibers. Ubx^Scer\UAS.cCa expression in all muscle cells at the onset of metamorphosis under the control of Scer\GAL4^how-24B (and Scer\GAL80^ts.αTub84B, placed at the restrictive temperature of 29^oC at the onset of metamorphosis) does not affect the differentiation of adult ostiae that develop from A1-A5 svp-expressing cells. (Monier et al., 2005) Expression of Ubx[Scer\UAS.cCa] under the control of Scer\GAL4[dpp.blk1] results in transformation of the arista to tarsus. (Percival-Smith et al., 2005) In stage 16 Ubx^Scer\UAS.cCa; Scer\GAL4^how-24B embryos, the number of cells in the dorsal vessel is increased from 104 to 116 cells compared to wild-type. (Ryan et al., 2005) When clones expressing Ubx^Scer\UAS.cCa (driven by Scer\GAL4^αTub84B.PL) in the posterior of eye disc, photoreceptor differentiation is blocked . (Janody et al., 2004) In stage-15 mutant embryos in which Ubx^Scer\UAS.cCa is overexpressed under the control of Scer\GAL4^Mef2.PR, lymph-gland progenitors are replaced by pericardial nephrocytes. (Mandal et al., 2004) In mutant embryos expressing Ubx^Scer\UAS.cCa driven by both Scer\GAL4^twi.PG and Scer\GAL4^how-24B ectopic cardioblasts are seen and the lymph glands are systematically eliminated and replaced by major pericardial cells. The anterior aorta is also transformed into a posterior aorta and heart tissue. (Perrin et al., 2004) Expression of Ubx^Scer\UAS.cCa under the control of Scer\GAL4^sca-537.4 results in a mutant phenotype in the embryonic tritocerebrum. The phenotype has a penetrance of more than 95%. (Sprecher et al., 2004) Postembryonic neuroblast (pNB) clones in the larval thorax expressing Ubx^Scer\UAS.cCa under the control of Scer\GAL4^αTub84B.PL are reduced in size compared to wild-type clones and the clones show loss of the pNB in at least 82% of cases. (Bello et al., 2003) The number of peripodial membrane cells in the wing discs of Ubx^Scer\UAS.cCa; Scer\GAL4^vg.PM late 3rd instar larvae is decreased to less than 250 (from 400-450 in wild-type, wheras with Ubx^Scer\UAS.cCa; Scer\GAL4⁴²⁶ it is increased to >550. (Pallavi and Shashidhara, 2003) Expression of Ubx^Scer\UAS.cCa under the control of Scer\GAL4^en-e16E does not result in the formation of ectopic oenocytes in the T1-T3 segments. (Brodu et al., 2002) Expression of Ubx^Scer\UAS.cCa under the control of Scer\GAL4^arm.PS in embryos results in the transformation of segment T3 into A1. The penetrance of this phenotype is 100%. (Galant and Carroll, 2002) Expression of Ubx^Scer\UAS.cCa under the control of Scer\GAL4^arm.PS in embryos results in the transformation of the identity of third thoracic segment to that of abdominal segment A1. (Galant et al., 2002) Expression of Ubx^Scer\UAS.cCa under the control of Scer\GAL4^how-24B in embryos does not inhibit heart cell specification in segments A5-A8. There is often an increase in cell size in the dorsal vessel at the A4/A5 boundary, consistent with the formation of an ectopic ostium within the aorta. (Lovato et al., 2002) Cardioblasts do not have normal morphology in embryos expressing Ubx^Scer\UAS.cCa under the control of both Scer\GAL4^twi.PG and Scer\GAL4^how-24B and the ostiae are not functional. (Ponzielli et al., 2002) 94% of flies expressing Ubx^Scer\UAS.cCa under the control of Scer\GAL4^ey.PH are wild type. Some animals are seen in which most of the head and one or both eyes of reduced size are present. (Jiao et al., 2001) Overexpression of Ubx^Scer\UAS.cCa driven by Scer\GAL4^dpp.blk1 causes transformation of the antenna towards a thoracic leg. Overexpression of Ubx^Scer\UAS.cCa driven by Scer\GAL4^Bx-MS1096 causes transformation of the wing towards a haltere in females. Males do not survive larval stages. The phenotype is stronger on the dorsal surface of the wing. Overexpression of Ubx^Scer\UAS.cCa in the ectoderm, driven by Scer\GAL4^arm.PS, transforms the denticle belts of thoracic segments towards A1 identity. The development of Keilin's organs and ventral pits are repressed. (Grenier and Carroll, 2000) When ectopically expressed by a strong Scer\GAL4^arm.PS (arm-GAL⁴), Ubx^Scer\UAS.cCa transforms head and all three thoracic segments into morphological replicas of A1 i.e. generating A1-like denticle belts in these more anterior segments. T1 develops without the characteristic patch of beard denticles. When ectopically expressed by a weak Scer\GAL4^arm.PS (arm-GAL^4r), Ubx^Scer\UAS.cCa shows no abnormalities at all. (Li and McGinnis, 1999) The overall shape of the central nervous system is not perturbed in embryos expressing Ubx^Scer\UAS.cCa under the control of Scer\GAL4^Mz1407. The pattern of BrdU incorporation in the central nervous system is abnormal in stage 16/17 embryos; the lateral replicating clusters of the thoracic neuromeres are abolished, and ectopic sites of BrdU incorporation are seen in the ventral region of the thoracic neuromeres. (Prokop et al., 1998) Scer\GAL4^twi.PG induced expression causes ectopic gonadal mesoderm formation in anterior segments of the embryo. Df(3R)Ubx109/Df(3R)P9 embryos lack gonads, ectopic expression of Ubx restores the formation of an encapsulated gonad. (Greig and Akam, 1995) Keilin's organs are suppressed in the Ubx^Scer\UAS.cCa, Scer\GAL4^h-1J3 or Ubx^Scer\UAS.cCa, Scer\GAL4^Kr.PM combination. With Scer\GAL4^69B, posterior spiracles are reduced to half their wild type size, in most embryos a small A9 denticle belt is formed anterior to the anal plates and the anal tuft is often reduced. Similar but weaker effects on the posterior spiracles are seen with Scer\GAL4^h-1J3. (Castelli-Gair et al., 1994)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement Reference Scer\GAL4^dpp.blk1, Ubx^Scer\UAS.cCa has visible phenotype, suppressible by hth^Scer\UAS.cPa/Scer\GAL4^dpp.blk1 (Yao et al., 1999) Scer\GAL4^dpp.blk1, Ubx^Scer\UAS.cCa has visible phenotype, suppressible by pb^{Scer\UAS.T:Avic\GFP-EGFP}/Scer\GAL4^dpp.blk1 (Percival-Smith et al., 2005)
Phenotype Manifest In
Suppressed by
Statement Reference Scer\GAL4^dpp.blk1, Ubx^Scer\UAS.cCa has antenna phenotype, suppressible by hth^Scer\UAS.cPa/Scer\GAL4^dpp.blk1 (Yao et al., 1999) Scer\GAL4^dpp.blk1, Ubx^Scer\UAS.cCa has arista phenotype, suppressible by pb^{Scer\UAS.T:Avic\GFP-EGFP}/Scer\GAL4^dpp.blk1 (Percival-Smith et al., 2005) Scer\GAL4^dpp.blk1, Ubx^Scer\UAS.cCa has pretarsus \| ectopic phenotype, suppressible by pb^{Scer\UAS.T:Avic\GFP-EGFP}/Scer\GAL4^dpp.blk1 (Percival-Smith et al., 2005) Ubx^Scer\UAS.cCa has embryonic tritocerebrum phenotype, suppressible by hth^Scer\UAS.cPa/Scer\GAL4^sca-537.4/exd^{Scer\UAS.NLS.T:Zzzz\FLAG} (Sprecher et al., 2004)
Additional Comments
Genetic Interactions
Statement Reference Co-expression of pb[Scer\UAS.T:Avic\GFP-EGFP] suppresses the arista-to-tarsus transformation seen in flies expressing Ubx[Scer\UAS.cCa] under the control of Scer\GAL4[dpp.blk1]. (Percival-Smith et al., 2005) The defect in the embryonic tritocerebrum caused by expression of Ubx^Scer\UAS.cCa under the control of Scer\GAL4^sca-537.4 is completely suppressed if the embryos are also coexpressing both exd^{Scer\UAS.NLS.T:Zzzz\FLAG} and hth^Scer\UAS.cPa. (Sprecher et al., 2004) Co-expression of hth^Scer\UAS.cPa partially suppresses the antenna to leg transformation phenotypes produced by ectopic expression of Ubx^Scer\UAS.cCa driven by Scer\GAL4^dpp.blk1. The antennae produced are aristaless and occasionally duplicated, similar to the hth^Scer\UAS.cPa phenotype. (Yao et al., 1999) The segmentation phenotype of Df(3R)Ubx109 is modified in the Ubx^Scer\UAS.cCa, Scer\GAL4^h-1J3 combination to transform alternate segments to resemble a wild type A1. This transformation is complete in the Ubx^Scer\UAS.cCa, Scer\GAL4^69B combination. The sensory organ phenotype of Df(3R)Ubx109 is modified in the Ubx^Scer\UAS.cCa, Scer\GAL4^h-1J3 or Ubx^Scer\UAS.cCa, Scer\GAL4^Kr.PM combination to prevent the ventral migration of the cells that would form the kolbchen, though later differentiation produces misplaced kolbchen rather than dorsal hairs. The sensory organ phenotype of Df(3R)Ubx109 is modified in the Ubx^Scer\UAS.cCa, Scer\GAL4^69B combination to affect the migration and differentiation of the kolbchen. (Castelli-Gair et al., 1994)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments	The partial transformation of haltere into wing which is seen in Ubx[LDN]/Ubx[abx-1] Ubx[bx-3] Ubx[pbx-1] flies is strongly suppressed by expression of Ubx[Scer\UAS.cCa] under the control of Scer\GAL4[Ubx-LDN]. (de Navas et al., 2011) Expression of Ubx^Scer\UAS.cCa rescues the phenotype of Ubx^LDN, Ubx^bx-3, Ubx^pbx-1, Ubx^abx-1 flies. (de Navas et al., 2006)
Stocks ( 2 )
Bloomington	911 w¹; P{UAS-Ubx.Ia.C}36.2/TM3, Ser¹
Kyoto	106118 w¹; P{UAS-Ubx.Ia.C}36.2/TM3, Ser¹
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	Ubx^Scer\UAS.cCa Ubx^UAS.cCa
Name Synonym	Saccharomyces cerevisiae UAS construct a of Castelli-Gair (Castelli-Gair et al., 1994)
Secondary FlyBase IDs

References ( 42 )

Generate a list of	All references relating to this allele ( 42 )

List References by type	Research paper ( 42 )
Recent research papers ( 3 )
	Ahn et al., 2011, Dev. Biol. 355(2): 336--348 Segment-specific regulation of the Drosophila AP-2 gene during leg and antennal development. [FBrf0213960] de Navas et al., 2011, Development 138(1): 107--116 Integration of RNA processing and expression level control modulates the function of the Drosophila Hox gene Ultrabithorax during adult development. [FBrf0212483] Tripura et al., 2011, Int. J. Dev. Biol. 55(6): 583--590 Regulation and activity of JNK signaling in the wing disc peripodial membrane during adult morphogenesis in Drosophila. [FBrf0216296] Show all research papers ...

Allele Dmel\UbxScer\UAS.cCa

Allele Dmel\Ubx^Scer\UAS.cCa