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General Information
Symbol
Dmel\abd-A1dB5.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0039121
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-abd-A, UAS-abdA
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

abd-A cDNA expressed under influence of UASt regulatory sequences.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In

embryonic abdominal segment 1 & somatic muscle, with Scer\GAL4how-24B

embryonic thoracic segment & somatic muscle, with Scer\GAL4how-24B

Detailed Description
Statement
Reference

Expression of abd-A1dB5.Scer\UAS driven by Scer\GAL4svp.PT causes ostia cells to be more elongated; this effect is even stronger when driven by Scer\GAL4twi.PG.

Scer\GAL4how-24B-mediated expression of abd-A1dB5.Scer\UAS results in an ectopic DA3 muscle in thoracic segment 1. The number of nuclei in this ectopic muscle is identical to that in wild type abdominal segments.

Embryos ectopically expressing Scer\GAL4how-24B driven abd-A1dB5.Scer\UAS throughout the mesoderm show an increased number of alary muscle pairs, from the seven normally observed in wild-type, to approximately ten. The additional muscles are located in the more anterior region.

Expression of abd-A1dB5.Scer\UAS under the control of Scer\GAL4elav-C155 results in neuroblast apoptosis in the thorax and abdomen in larvae at 96 hours.

Expression of abd-A1dB5.Scer\UAS under the control of Scer\GAL4αTub84B.PL in clones in the larval central nervous system results in clones that are smaller than wild-type controls and which comprise only small cells (neuroblasts are lacking).

Postembryonic neuroblast (pNB) clones in the larval thorax expressing abd-A1dB5.Scer\UAS under the control of Scer\GAL4elav-C155 and Scer\GAL4αTub84B.PL are reduced in size compared to wild-type clones (from 58 to 20 neurons). In most of the mutant clones, no identifiable neuroblast is visible at 96 hours after larval hatching. Cells in the clones are always located close to the neuropil and have a morphology typical of early-born neurons. Postembryonic neuroblast (pNB) clones in the larval thorax expressing abd-A1dB5.Scer\UAS under the control of Scer\GAL4αTub84B.PL are reduced in size compared to wild-type clones and the clones show loss of the pNB in at least 82% of cases.

Expression of abd-A1dB5.Scer\UAS under the control of Scer\GAL4en-e16E results in the formation of ectopic oenocytes in the T1-T3 segments (they are normally only found in segments A1-A7). Expression of abd-A1dB5.Scer\UAS under the control of Scer\GAL4ato.3.6 results in the formation of ectopic oenocytes in the T1-T3 segments. Expression of abd-A1dB5.Scer\UAS under the control of Scer\GAL4salm-459.2 does not result in the formation of ectopic oenocytes in the T1-T3 segments.

Embryos expressing abd-A1dB5.Scer\UAS under the control of Scer\GAL469B do not undergo dorsal closure to form a linear heart tube, although heart and aorta cells can still be distinguished. Expression of abd-A1dB5.Scer\UAS under the control of Scer\GAL4how-24B or Scer\GAL4twi.PG results in transformation of the aorta into heart. The lumen of the dorsal vessel is wider than normal. Variations in dorsal vessel diameter similar to that normally found in the heart are seen in anterior locations in embryos expressing abd-A1dB5.Scer\UAS under the control of Scer\GAL4twi.PG and occasionally tiny perforations in the heart wall are seen, consistent with the interpretation that ectopic ostia are being formed in anterior locations.

Scer\GAL4E132 induced expression in the eye-antennal disc causes transformation of the antenna into leg. Scer\GAL4Bx-MS1096 or Scer\GAL4C-765 induced expression in the wing disc causes transformation of the wing into haltere-like tissue. Scer\GAL4C-765 and Scer\GAL4pnr-MD237 induced expression also transforms the mesonotum and metanotum into an abdominal segment. Scer\GAL4c739 and Scer\GAL4C-765 induced expression in the abdomen transforms A1 into a posterior abdominal segment.

When expressed via Scer\GAL4how-24B in embryos, an ectopic precursor for abdominal muscle 26 appears in the ventral region of T1-T3. Ventral longitudinal muscles of thoracic segments assume the identity of the abdominal segments. Abdominal segment 1 often gain two muscles that it normally lacks: external ventral oblique muscles 17 and 25. Some atypical muscles appear in T1 and T2. Insertion sites of transformed muscles are sometimes aberrant. The thoracic cuticle remains unaffected. There is no affect on the development of the gut.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Suppressed by
NOT suppressed by
Statement
Reference
Suppressor of
NOT Suppressor of
Additional Comments
Genetic Interactions
Statement
Reference

The neuroblast apoptosis seen in the thorax and abdomen of 96 hour larvae expressing abd-A1dB5.Scer\UAS under the control of Scer\GAL4elav-C155 is suppressed by co-expression of casScer\UAS.cKa.

Expression of abd-A1dB5.Scer\UAS results in an almost complete rescue of the transformed haltere phenotype of Ubxabx-1 Ubxbx-3 Ubxpbx-1/UbxLDN flies. The P{GawB}UbxLDN insertion within the UbxLDN flies drives expresssion of abd-A1dB5.Scer\UAS.

Expression of abd-A1dB5.Scer\UAS cannot suppress the absence of genitalia in Abd-BLDN/Abd-BM1 female flies, not the presence of a large seventh tergite in both male and female Abd-BLDN/Abd-BM1 flies. abd-A1dB5.Scer\UAS expression is driven by the P{GawB}Abd-BLDN insertion in the Abd-BLDN flies.

Postembryonic neuroblast (pNB) clones in the larval thorax expressing abd-A1dB5.Scer\UAS under the control of Scer\GAL4elav-C155 and Scer\GAL4αTub84B.PL and that are also mutant for Df(3L)H99 continue to proliferate throughout larval life and adopt the large size typical of wild-type lineages in the thorax. All these mutant clones retain their pNB at 96 hours after larval hatching.

Co-expression of hthScer\UAS.cPa partially suppresses the antenna to leg transformation phenotypes produced by ectopic expression of abd-A1dB5.Scer\UAS driven by Scer\GAL4dpp.blk1. The antennae produced are aristaless and occasionally duplicated, similar to the hthScer\UAS.cPa phenotype.

Co-expression of exdScer\UAS.cGa (induced by Scer\GAL4Bx-MS1096) does not significantly modify the wing transformation. Ubxabx-1 Ubxbx-3 Ubxpbx-1/Ubxlac1 combination transforms the metathorax into mesothorax (including transformation of halteres into wings), Scer\GAL4Bx-MS1096 induced expression allows full rescue.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
Comments
Comments

Expression dependent on Scer\GAL4.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
abd-A1dB5.Scer\UAS
abd-A1dB5.UAS
Name Synonyms
Secondary FlyBase IDs
    References (21)