Allele Dmel\en^hs.3

General Information
Symbol	Dmel\en^hs.3	Species	D. melanogaster
Name		FlyBase ID	FBal0039318
Feature type	allele	Associated gene	Dmel\en

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Poole and Kornberg, 1988, Serrano et al., 1995, Bourbon et al., 1995, Grether et al., 1995, Serrano et al., 1997, Maschat et al., 1998, Serrano and Maschat, 1998)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference en is expressed under the control of an Hsp70 promoter. (Serrano et al., 1997) A heat shock promoter drives en expression. (Bourbon et al., 1995) Expression of a fragment containing the en transcription unit is driven by an Hsp70 promoter. (Grether et al., 1995) en coding sequence is driven by an Hsp70 promoter. (Serrano et al., 1995) Construct: Fusion of 2kb EcoRI fragment of en cDNA and 2.1kb fragment of en genomic DNA, including the polyadenylation region, is expressed from an Hsp70 promoter. (Poole and Kornberg, 1988)
Carried in construct	P{hs-en3} (Deshpande et al., 2001, Bourbon et al., 1995, Serrano et al., 1995, Macias et al., 1994, Siegler and Jia, 1999, Poole and Kornberg, 1988, Serrano and Maschat, 1998, Maschat et al., 1998, Saenz-Robles et al., 1995, Grether et al., 1995, Serrano et al., 1997)
Cytology
Phenotypic Data
Phenotypic Class
	lethal \| heat sensitive (Poole and Kornberg, 1988)
Phenotype Manifest In
	cephalopharyngeal skeleton \| heat sensitive (Poole and Kornberg, 1988) denticle belt \| heat sensitive (Poole and Kornberg, 1988) EL neuron (Deshpande et al., 2001) embryonic epidermis \| heat sensitive (Poole and Kornberg, 1988) embryonic head \| heat sensitive (Poole and Kornberg, 1988) neuroblast NB7-3 \| ectopic (Deshpande et al., 2001) presumptive embryonic/larval central nervous system (Deshpande et al., 2001)
Detailed Description
	Statement Reference Expression of en^hs.3 using a heat pulse 5-6 hours after egg laying at 25^oC results in ectopic NB 7-3 formation in 20% of hemisegments. The ectopic NB 7-3 is also able to give rise to characteristic progeny cells; additional serotonergic neurons are seen in 20% of the hemineuromeres of first instar larvae. A loss of eve-positive EL cells is seen. No additional NB 7-3 cluster is seen when en^hs.3 is expressed using a heat pulse 4-5 hours after egg laying at 25^oC, although the central nervous system is very malformed. (Deshpande et al., 2001) Failed to induce programmed cell death (PCD) in Df(3L)H99 embryos. (Grether et al., 1995) Heat shock during cellular blastoderm and early gastrulation causes pattern defects similar to those in embryos lacking en function. Heat shock during cellular blastoderm causes variable head defects, missing mouthparts, and abnormal, fused or missing denticle belts. Heat shock during gastrulation causes a series of defects in segmentation, from denticle belt fusions to a pair rule-type phenotype. Most prevalent fusions are A2 fused to A3, A4 fused to A5 and A6 fused to A7, similar to en⁴. (Poole and Kornberg, 1988)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 1 )
Bloomington	3516 P{hs-en3}3; ry^*
Notes on Origin
Discoverer

Comments
	Temperature sensitive period in the embryo includes cellular blastoderm and early gastrulation only. (Poole and Kornberg, 1988)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	en^hs.3 en^hs.PP
Name Synonym
Secondary FlyBase IDs

References ( 12 )
Research paper	Deshpande et al., 2001, Development 128(17): 3253--3261 Successive specification of Drosophila neuroblasts NB 6-4 and NB 7-3 depends on interaction of the segment polarity genes wingless, gooseberry and naked cuticle. [FBrf0138370] Siegler and Jia, 1999, Neuron 22(2): 265--276 Engrailed negatively regulates the expression of cell adhesion molecules connectin and neuroglian in embryonic Drosophila nervous system. [FBrf0107991] Maschat et al., 1998, Development 125(15): 2771--2780 engrailed and polyhomeotic interactions are required to maintain the A/P boundary of the Drosophila developing wing. [FBrf0103326] Serrano and Maschat, 1998, EMBO J. 17(13): 3704--3713 Molecular mechanism of polyhomeotic activation by engrailed. [FBrf0103370] Serrano et al., 1997, Development 124(13): 2527--2536 3-tubulin is directly repressed by the engrailed protein in Drosophila. [FBrf0096276] Bourbon et al., 1995, J. Biol. Chem. 270(19): 11130--11139 Phosphorylation of the Drosophila engrailed protein at a site outside its homeodomain enhances DNA binding. [FBrf0081478] Grether et al., 1995, Genes Dev. 9(14): 1694--1708 The head involution defective gene of Drosophila melanogaster functions in programmed cell death. [FBrf0082050] Saenz-Robles et al., 1995, Mech. Dev. 53(2): 185--195 Selection and characterization of sequences with high affinity for the engrailed protein of Drosophila. [FBrf0084332] Sanicola et al., 1995, Genetics 139(2): 745--756 Drawing a stripe in Drosophila imaginal disks: Negative regulation of decapentaplegic and patched expression by engrailed. [FBrf0080369] Serrano et al., 1995, Development 121(6): 1691--1703 polyhomeotic appears to be a target of engrailed regulation in Drosophila. [FBrf0079388] Macias et al., 1994, Mech. Dev. 46(1): 15--25 Genetic factors controlling the expression of the abdominal-A gene of Drosophila within its domain. [FBrf0073799] Poole and Kornberg, 1988, French, Ingham, Cooke, Smith, 1988: 85--93 Modifying expression of the engrailed gene of Drosophila melanogaster. [FBrf0047901]

Allele Dmel\enhs.3

Allele Dmel\en^hs.3