argoshs.PF mutant embryos have about 86 cells in each Malpighian tubule cell (as opposed to about 125 in wild-type).
The number of midline glia cells per segment that survive embryogenesis is decreased in embryos expressing argoshs.PF. The most sensitive period for alteration of MG number is between stages 12 and 16. Some narrowing of the nerve cord is seen in stage 16 embryos subjected to three 45 minute heat shocks (separated by 45 minutes) beginning at stage 12. The commissural tracts are less uniform and slightly wider than wild-type. The axons of the commissural tracts are not ensheathed, and show some indication of membrane damage or loss on the exposed dorsal surface of the commissure.
wing veins L2, L4 and L5 are truncated in argoshs.PF/+ animals heat-shocked at 34oC.
Heat induced expression between 24 hours and 32 hours after puparium formation causes fewer than normal retinula cells in the ommatidium and loss of pigment cells between different ommatidia.
Heat shock during the third larval instar is lethal. Heat shock during pupal stages is not fully lethal, and if administered during the first 30 hours of pupation, causes severe disruptions in eye development. Ommatidial regularity is disrupted and fusions occur. Surviving pupae heat shocked before 12 hours post-pupariation have a zone of roughness in the anterior portion of the eye. Heat shocks after 24 hours post pupariation cause less lethality and considerable eye roughening, peaking at 28 hours. Heat shock after 32 hours post-pupariation has no effect on the eye. Early heat shocks cause loss of photoreceptors and cone cells. Later shocks cause loss of secondary and tertiary pigment cells.