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General Information
Symbol
Dmel\aoshs.PF
Species
D. melanogaster
Name
heat shock construct of Freeman
FlyBase ID
FBal0039368
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

2.4kb fragment of argos cDNA 159, expression is influenced by Hsp70 5' and 3' sequences.

aos minigene, with large first intron removed, expressed from an Hsp70 promoter.

Allele components
Product class / Tool use(s)
Regulatory region(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

argoshs.PF mutant embryos have about 86 cells in each Malpighian tubule cell (as opposed to about 125 in wild-type).

Rough eye phenotype.

The number of midline glia cells per segment that survive embryogenesis is decreased in embryos expressing argoshs.PF. The most sensitive period for alteration of MG number is between stages 12 and 16. Some narrowing of the nerve cord is seen in stage 16 embryos subjected to three 45 minute heat shocks (separated by 45 minutes) beginning at stage 12. The commissural tracts are less uniform and slightly wider than wild-type. The axons of the commissural tracts are not ensheathed, and show some indication of membrane damage or loss on the exposed dorsal surface of the commissure.

wing veins L2, L4 and L5 are truncated in argoshs.PF/+ animals heat-shocked at 34oC.

Heat induced expression between 24 hours and 32 hours after puparium formation causes fewer than normal retinula cells in the ommatidium and loss of pigment cells between different ommatidia.

Heat shock during the third larval instar is lethal. Heat shock during pupal stages is not fully lethal, and if administered during the first 30 hours of pupation, causes severe disruptions in eye development. Ommatidial regularity is disrupted and fusions occur. Surviving pupae heat shocked before 12 hours post-pupariation have a zone of roughness in the anterior portion of the eye. Heat shocks after 24 hours post pupariation cause less lethality and considerable eye roughening, peaking at 28 hours. Heat shock after 32 hours post-pupariation has no effect on the eye. Early heat shocks cause loss of photoreceptors and cone cells. Later shocks cause loss of secondary and tertiary pigment cells.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement
Reference

aoshs.PF has visible phenotype, suppressible by ed[+]/ed1X5

Phenotype Manifest In
Suppressed by
Statement
Reference

aoshs.PF has eye phenotype, suppressible by ed[+]/ed1X5

aoshs.PF has wing vein phenotype, suppressible by EgfrWT.hs

Additional Comments
Genetic Interactions
Statement
Reference

EgfrWT.hs/+ suppresses the wing-vein loss phenotype of argoshs.PF/+ animals heat-shocked at 34o.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Partially rescues

aoshs.PF partially rescues aos5

Comments

Rescues the eye defect of argos5, though incompletely.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
aoshs.PF
argoshs.PF
gilhs.PF
Name Synonyms
heat shock construct of Freeman
Secondary FlyBase IDs
    References (7)