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General Information
Symbol
Dmel\aoshs.PSa
Species
D. melanogaster
Name
heat shock construct a of Sawamoto
FlyBase ID
FBal0039369
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
hs-argos
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

Expression of a 2kb EcoRI cDNA fragment of aos including the complete coding region is driven by a Hsp70 promoter.

Allele components
Product class / Tool use(s)
Regulatory region(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Flies expressing argoshs.PSa have an eye phenotype and show loss of wing veins.

Expression of argoshs.PSa using multiple heat shocks during larval stages does not result in an increase in the number of mature R8 cells.

When argoshs.PSa females are treated with heatshock, eggs laid over the next three days have a range of polarity defects. Eggs laid early show a lateralized phenotype, with a large single dorsal appendage. Behind the large appendage, the operculum is missing and there is a space where no chorion is deposited. Eggs laid later exhibit a ventralized phenotype - there is a ring like structure surrounding the micropyle in the anterior of these embryos. A few eggs (about 1%) have two anteriors in mirror image symmetry, but no posterior. The duplicated anterior is not identical to the normal one but sometimes has a smaller pseudo-micropyle and a ring like structure surrounding it.

The number of midline glia cells per segment that survive embryogenesis is decreased in embryos expressing argoshs.PSa. The most sensitive period for alteration of MG number is between stages 12 and 16. Some narrowing of the nerve cord is seen in stage 16 embryos subjected to three 45 minute heat shocks (separated by 45 minutes) beginning at stage 12. The commissural tracts are less uniform and slightly wider than wild-type. The axons of the commissural tracts are not ensheathed, and show some indication of membrane damage or loss on the exposed dorsal surface of the commissure.

Heat shocked embryos exhibit loss of one or two Ch organs at LCh5.

Severe rough eye phenotype and shortened longitudinal veins.

Fully penetrant rough eye phenotype at 25oC.

Expression in a wild type background causes a rough eye and wing phenotypes. Phenotype is enhanced by increasing copy number. Expression of argoshs.PSa using heat pulses from the second instar to pupal stages results in wings in which veins L2, L4 and L5 are shortened and crossveins are missing. L3 is unaffected in most cases.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference

aoshs.PSa has visible phenotype, enhanceable by EM3-1EM3-1

aoshs.PSa has visible phenotype, enhanceable by SEM2-4

aoshs.PSa has visible phenotype, enhanceable by SEF2-1

aoshs.PSa has visible phenotype, enhanceable by rhove-1

Suppressed by
Statement
Reference

aoshs.PSa has visible phenotype, suppressible by SM2-1SM2-1

aoshs.PSa has visible phenotype, suppressible by bulSF3-1

aoshs.PSa has visible phenotype, suppressible by clnSF3-2

aoshs.PSa has visible phenotype, suppressible by clnSF3-3

aoshs.PSa has visible phenotype, suppressible by SM3-2SM3-2

aoshs.PSa has visible phenotype, suppressible by stySM3-5

aoshs.PSa has visible phenotype, suppressible by stySM3-9

aoshs.PSa has visible phenotype, suppressible by SM3-3SM3-3

aoshs.PSa has visible phenotype, suppressible by SM3-6SM3-6

aoshs.PSa has visible phenotype, suppressible by SM3-8SM3-8

Enhancer of
Statement
Reference

aoshs.PSa is an enhancer of visible phenotype of rgunspecified

aoshs.PSa is an enhancer of visible phenotype of rhove-1

Phenotype Manifest In
Enhanced by
Statement
Reference

aoshs.PSa has eye phenotype, enhanceable by EM3-1EM3-1

aoshs.PSa has eye phenotype, enhanceable by SEM2-4

aoshs.PSa has wing phenotype, enhanceable by SEM2-4

aoshs.PSa has wing vein phenotype, enhanceable by SEF2-1

aoshs.PSa has eye phenotype, enhanceable by SEF2-1

aoshs.PSa has phenotype, enhanceable by SX155

aoshs.PSa has phenotype, enhanceable by S218

aoshs.PSa has phenotype, enhanceable by bulD

aoshs.PSa has wing vein phenotype, enhanceable by rhove-1

Suppressed by
Statement
Reference

aoshs.PSa has eye phenotype, suppressible by SM2-1SM2-1

aoshs.PSa has wing phenotype, suppressible by SM3-8SM3-8

aoshs.PSa has eye phenotype, suppressible by bulSF3-1

aoshs.PSa has wing vein phenotype, suppressible by bulSF3-1

aoshs.PSa has eye phenotype, suppressible by clnSF3-2

aoshs.PSa has wing phenotype, suppressible by clnSF3-2

aoshs.PSa has eye phenotype, suppressible by clnSF3-3

aoshs.PSa has wing phenotype, suppressible by clnSF3-3

aoshs.PSa has eye phenotype, suppressible by stySM3-5

aoshs.PSa has wing phenotype, suppressible by stySM3-5

aoshs.PSa has eye phenotype, suppressible by stySM3-9

aoshs.PSa has wing phenotype, suppressible by stySM3-9

aoshs.PSa has eye phenotype, suppressible by SM3-2SM3-2

aoshs.PSa has eye phenotype, suppressible by SM3-3SM3-3

aoshs.PSa has eye phenotype, suppressible by SM3-6SM3-6

aoshs.PSa has eye phenotype, suppressible by SM3-8SM3-8

aoshs.PSa has phenotype, suppressible by Dsor1Su1

aoshs.PSa has phenotype, suppressible by rlSu14

aoshs.PSa has phenotype, suppressible by rlSu23

aoshs.PSa has phenotype, suppressible by bul23a1

aoshs.PSa has phenotype, suppressible by bul28a1

aoshs.PSa has phenotype, suppressible by bul6d7

aoshs.PSa has phenotype, suppressible by cln1

aoshs.PSa has phenotype, suppressible by soba5a1

Enhancer of
Statement
Reference

aoshs.PSa is an enhancer of eye phenotype of rgunspecified

aoshs.PSa is an enhancer of wing vein phenotype of rhove-1

Suppressor of
Statement
Reference

aoshs.PSa is a suppressor of phenotype of rlSu23

Other
Additional Comments
Genetic Interactions
Statement
Reference

The argoshs.PSa phenotype can be suppressed by Dsor1Su1, rlSu14 and rlSu23, and enhanced by SX155 and S218. Overexpression of argos fails to affect the multiple R7 phenotype of sevS11.T:Hsap\MYC.

rhove-1 homozygotes which are also expressing argoshs.PSa (using heat pulses from the second and third larval instar to pupal stage) lack most of wing veins L2-L4 and the entire L5 vein is eliminated. The campaniform sensilla on vein L3 usually remain intact.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

Rescues the retinal axon phenotype of argosp2, when heat shock is administered through the larval stages.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
aoshs.PSa
argoshs.PSa
gilhs.PSa
Name Synonyms
heat shock construct a of Sawamoto
Secondary FlyBase IDs
  • FBal0039370
References (12)