Flies expressing argoshs.PSa have an eye phenotype and show loss of wing veins.
Expression of argoshs.PSa using multiple heat shocks during larval stages does not result in an increase in the number of mature R8 cells.
When argoshs.PSa females are treated with heatshock, eggs laid over the next three days have a range of polarity defects. Eggs laid early show a lateralized phenotype, with a large single dorsal appendage. Behind the large appendage, the operculum is missing and there is a space where no chorion is deposited. Eggs laid later exhibit a ventralized phenotype - there is a ring like structure surrounding the micropyle in the anterior of these embryos. A few eggs (about 1%) have two anteriors in mirror image symmetry, but no posterior. The duplicated anterior is not identical to the normal one but sometimes has a smaller pseudo-micropyle and a ring like structure surrounding it.
The number of midline glia cells per segment that survive embryogenesis is decreased in embryos expressing argoshs.PSa. The most sensitive period for alteration of MG number is between stages 12 and 16. Some narrowing of the nerve cord is seen in stage 16 embryos subjected to three 45 minute heat shocks (separated by 45 minutes) beginning at stage 12. The commissural tracts are less uniform and slightly wider than wild-type. The axons of the commissural tracts are not ensheathed, and show some indication of membrane damage or loss on the exposed dorsal surface of the commissure.
Heat shocked embryos exhibit loss of one or two Ch organs at LCh5.
Severe rough eye phenotype and shortened longitudinal veins.
Fully penetrant rough eye phenotype at 25oC.
Expression in a wild type background causes a rough eye and wing phenotypes. Phenotype is enhanced by increasing copy number. Expression of argoshs.PSa using heat pulses from the second instar to pupal stages results in wings in which veins L2, L4 and L5 are shortened and crossveins are missing. L3 is unaffected in most cases.