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General Information
Symbol
Dmel\grpunspecified
Species
D. melanogaster
Name
FlyBase ID
FBal0039379
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Mutagen
    Nature of the Allele
    Allele class
    Mutagen
    Mutations Mapped to the Genome
     
    Type
    Location
    Additional Notes
    References
    Associated Sequence Data
    DNA sequence
    Protein sequence
     
     
    Progenitor genotype
    Cytology
    Nature of the lesion
    Statement
    Reference
    Expression Data
    Reporter Expression
    Additional Information
    Statement
    Reference
     
    Marker for
    Reflects expression of
    Reporter construct used in assay
    Human Disease Associations
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Disease
    Interaction
    References
    Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
     
    Disease-implicated variant(s)
     
    Phenotypic Data
    Phenotypic Class
    Phenotype Manifest In

    interphase & organism | embryonic cycle 11 | maternal effect

    interphase & organism | embryonic cycle 12 | maternal effect

    interphase & organism | embryonic cycle 13 | maternal effect

    Detailed Description
    Statement
    Reference

    Embryos derived from grpunspecified females have significantly shorter interphases during cycles 11, 12 and 13 compared to wild-type or lokp6 single mutant embryos.

    When exposed to increasing levels of X ray irradiation mutant animals exhibit similar levels of lethality to wild-type. However no decrease in mitotic index is seen in mutants, in contrast to wild-type, which show a gradual reduction of mitotic index with increasing X ray dose. Mutants also exhibit a slightly suppressed reduction of the frequency of anaphase seen when wild-type animals are exposed to increasing does ox X ray.

    No precocious anaphases are seen in grpunspecified mutants.

    In embryos derived from homozygous females mated to wild-type males, microtubule organisation is indistinguishable from that of wild-type embryos through the first 11 divisions. During mitosis 12, the spindles are somewhat shorter than wild type and the centrosomal foci are somewhat weaker than normal, but microtubule organisation during interphase following mitosis 12 appear normal. During mitosis 13 the centrosomal foci become essentially undetectable and anastral spindles with broad poles are formed. The chromosomes do not form a compact mass at the metaphase plate. Mitosis 13 is prolonged. On exit from mitosis 13, centrosomal foci reappear and organise interphase microtubule arrays.

    After interphase cycle 12, grp-derived embryos begin to exhibit an uneven nuclear distribution and irregular shaped nuclei. This phenotype becomes more extreme as the nuclei form distinct clusters. Cellularization does not occur. Abnormal nuclei do not recede into the interior. Fusion of sister nuclei occurs during telophase of nuclear cycle 12, and midbody formation is abnormal. Embryos arrest in cycle 13 with their chromosomes encompassed by spindles. Unusually, X irradiation induces metaphase arrest at all syncytial cycles.

    External Data
    Interactions
    Show genetic interaction network for Enhancers & Suppressors
    Phenotypic Class
    NOT Enhancer of
    Statement
    Reference

    grpunspecified is a non-enhancer of visible phenotype of Wee1GMR.PP

    grpunspecified is a non-enhancer of visible phenotype of Myt1GMR.PP

    NOT Suppressor of
    Statement
    Reference

    grpunspecified is a non-suppressor of visible phenotype of Wee1GMR.PP

    grpunspecified is a non-suppressor of visible phenotype of Myt1GMR.PP

    Phenotype Manifest In
    NOT Enhancer of
    Statement
    Reference

    grpunspecified is a non-enhancer of eye phenotype of Wee1GMR.PP

    grpunspecified is a non-enhancer of eye phenotype of Myt1GMR.PP

    grpunspecified is a non-enhancer of phenotype of pngunspecified

    NOT Suppressor of
    Statement
    Reference

    grpunspecified is a non-suppressor of eye phenotype of Wee1GMR.PP

    grpunspecified is a non-suppressor of eye phenotype of Myt1GMR.PP

    grpunspecified is a non-suppressor of phenotype of pngunspecified

    Other
    Statement
    Reference

    grpunspecified, mei-9a has oocyte nucleus & meiotic cell cycle phenotype

    Additional Comments
    Genetic Interactions
    Statement
    Reference

    grpunspecified, BubR1unspecified double mutants exhibit a mitotic index similar to BubR1unspecified when not irradiated, but do not exhibit as great a X ray dose dependent reduction in mitotic index as seen in BubR1unspecified.

    When mei-9a is added to either grpunspecified or grpunspecified/+ flies, precocious anaphases are sometimes seen.

    Xenogenetic Interactions
    Statement
    Reference
    Complementation and Rescue Data
    Comments
    Images (0)
    Mutant
    Wild-type
    Stocks (0)
    Notes on Origin
    Discoverer
    External Crossreferences and Linkouts ( 0 )
    Synonyms and Secondary IDs (2)
    Reported As
    Symbol Synonym
    grpunspecified
    unspecified
    Name Synonyms
    Secondary FlyBase IDs
      References (8)