Expression of hhScer\UAS.cCa suppresses the eye overgrowth phenotype seen when DlScer\UAS.cDa and mir-7Scer\UAS.cLb are expressed under the control of Scer\GAL4ey.PH. The phenotype is seen with 100% penetrance.
Co-expression of hhScer\UAS.cCa results in enlarged wing discs containing a larger field (30-40% of disc surface area) of differentiating ectopic eye precursors compared to expression of eyScer\UAS.cHa alone under the control of Scer\GAL4ap-md544.
When dppScer\UAS.cSa, eyScer\UAS.cHa and hhScer\UAS.cCa are driven by Scer\GAL430A, photoreceptor differentiation is induced in both compartments of the wing disc. When hhScer\UAS.cCa, eyaScer\UAS.cPa and eyScer\UAS.cHa are driven by Scer\GAL430A, photoreceptor differentiation is induced in the wing disc, only in the posterior compartment.
The wing phenotype caused by hhScer\UAS.cCa expressed under the control of Scer\GAL4en-e16E is suppressed by kncol-1, restoring an almost wild type position of wing vein L3 and its associated sensillae. An ectopic crossvein can be seen in the L2-L3 intervein.
ptcScer\UAS.cJa Scer\GAL471B flies carrying a relatively weakly expressing hhScer\UAS.cCa insertion line have wing discs that are indistinguishable from wild-type. ptcScer\UAS.cJa Scer\GAL471B flies carrying a more strongly expressing hhScer\UAS.cCa insertion line lack all veins in the anterior compartment, while vein 4 is present. Flies expressing both ptcScer\UAS.cJa and hhScer\UAS.cCa using Scer\GAL4MD-638 show recovery of the posterior compartment veins, ectopic induction of vein 3 and L3 sensilla and an enlargement of the region between veins 3 and 4.