Allele Dmel\in^IH56

General Information
Symbol	Dmel\in^IH56	Species	D. melanogaster
Name		FlyBase ID	FBal0039446
Feature type	allele	Associated gene	Dmel\in

Allele class	amorphic allele - genetic evidence
Mutagen	ethyl methanesulfonate
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class	amorphic allele - genetic evidence (Adler et al., 1994)
Mutagen	ethyl methanesulfonate (Adler et al., 1994)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference
Cytology
Phenotypic Data
Phenotypic Class
	planar polarity defective (Lu et al., 2010) planar polarity defective \| cell autonomous \| somatic clone (Lee and Adler, 2002) visible \| cold sensitive (Adler et al., 1994)
Phenotype Manifest In
	macrochaeta \| cold sensitive (Adler et al., 1994) microchaeta & wing (Park et al., 1996) wing hair (Lu et al., 2010) wing hair \| cell autonomous \| somatic clone (Lee and Adler, 2002) wing hair \| supernumerary (Lu et al., 2010)
Detailed Description
	Statement Reference Hairs in the proximal-anterior part of the wing point towards the anterior margin and most cells form multiple hairs in mutant adults. (Lu et al., 2010) Mutant clones in the wing show disruption of polarity, as indicated by wing hairs. (Lee and Adler, 2002) Unmarked clones in all regions of the wing display an in phenotype, cells form more than one hair and hairs with an abnormal polarity. Marked clones in the wing indicate in acts autonomously, cells bordering clones are wild type. P{in⁺12} construct can completely rescue the wing hair phenotype. Heat induced expression of P{hs-in} can provide nearly complete rescue of the wing hair phenotype, some multiple hairs are still present. Rescue of the in^IH56 mutant phenotype by P{hs-in(RGE)} is indistinguishable from rescue by P{hs-in}. (Park et al., 1996) Viable as a homozygote or a hemizygote. Strong thoracic bristle and wing hair phenotype. Percentage of dorsal wing surface cells that display abnormal cell polarity and the percentage of wing cells forming more than one hair is more severe at 18^oC than 29^oC. (Adler et al., 1994)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement Reference in^IH56, mwh¹ has planar polarity defective phenotype, suppressible \| partially by in::mwh^Scer\UAS.cLa/Scer\GAL4^ap-md544 (Lu et al., 2010) in^IH56 has planar polarity defective phenotype, suppressible by Scer\GAL4^Act.PU/in::mwh^Scer\UAS.cLa (Lu et al., 2010)
Suppressor of
Statement Reference in^IH56/in^unspecified is a suppressor of planar polarity defective \| somatic clone \| cell non-autonomous phenotype of fz²¹ (Lee and Adler, 2002)
NOT Suppressor of
Statement Reference in^IH56/in^unspecified is a non-suppressor of planar polarity defective \| somatic clone \| cell autonomous phenotype of fz²¹ (Lee and Adler, 2002) in^IH56 is a non-suppressor of planar polarity defective \| somatic clone \| cell non-autonomous phenotype of fz²¹ (Lee and Adler, 2002)
Other
Statement Reference Scer\GAL4^ptc-559.1, dsh^Scer\UAS.cAa, in^IH56 has planar polarity defective phenotype (Lee and Adler, 2002, Lee and Adler, 2002)
Phenotype Manifest In
Suppressed by
Statement Reference in^IH56, mwh¹ has wing hair \| supernumerary phenotype, suppressible \| partially by in::mwh^Scer\UAS.cLa/Scer\GAL4^ap-md544 (Lu et al., 2010) in^IH56, mwh¹ has wing hair phenotype, suppressible \| partially by in::mwh^Scer\UAS.cLa/Scer\GAL4^ap-md544 (Lu et al., 2010) in^IH56 has wing hair \| supernumerary phenotype, suppressible by Scer\GAL4^Act.PU/in::mwh^Scer\UAS.cLa (Lu et al., 2010) in^IH56 has wing hair phenotype, suppressible by Scer\GAL4^Act.PU/in::mwh^Scer\UAS.cLa (Lu et al., 2010)
Suppressor of
Statement Reference in^IH56/in^unspecified is a suppressor of wing hair \| somatic clone \| cell non-autonomous phenotype of fz²¹ (Lee and Adler, 2002)
NOT Suppressor of
Statement Reference in^IH56/in^unspecified is a non-suppressor of wing hair \| somatic clone \| cell autonomous phenotype of fz²¹ (Lee and Adler, 2002) in^IH56 is a non-suppressor of wing hair \| somatic clone \| cell non-autonomous phenotype of fz²¹ (Lee and Adler, 2002)
Other
Statement Reference Scer\GAL4^ptc-559.1, dsh^Scer\UAS.cAa, in^IH56 has macrochaeta phenotype (Lee and Adler, 2002, Lee and Adler, 2002) Scer\GAL4^ptc-559.1, dsh^Scer\UAS.cAa, in^IH56 has wing hair & 1st posterior cell phenotype (Lee and Adler, 2002, Lee and Adler, 2002)
Additional Comments
Genetic Interactions
Statement Reference The wing hair phenotype of in[IH56] mwh[1] double mutants is indistinguishable from that of mwh[1] single mutants. Expression of in::mwh[Scer\UAS.cLa] under the control of Scer\GAL4[Act.PU] essentially completely rescues the wing hair defects of in[IH56] mutants. Expression of in::mwh[Scer\UAS.cLa] under the control of Scer\GAL4[ap-md544] strongly rescues the wing hair defects of in[IH56] mwh[1] double mutants on the dorsal surface of the wing. (Lu et al., 2010) The multiple hair cell phenotype of dsh^Scer\UAS.cAa expressed from Scer\GAL4^ptc-559.1 is additive with the multiple hair phenotypes of fy² and in^IH56. When a wing clone is doubly mutant for fz²¹ and in^IH56, domineering nonautonomy of polarity defect of fz²¹ is unaffected. When a wing clone is doubly mutant for fz²¹ and in^IH56 in a genetic background deficient for in, domineering nonautonomy of polarity defect of fz²¹ is suppressed, though polarity defect within the clone is not. (Lee and Adler, 2002)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

Comments
	Strong in allele. (Adler et al., 1994)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	in^IH56 (Strutt and Warrington, 2008, Lu et al., 2010)
Name Synonym
Secondary FlyBase IDs

References ( 9 )
Research paper	Lu et al., 2010, Genetics 185(2): 549--558 The Drosophila planar polarity proteins inturned and multiple wing hairs interact physically and function together. [FBrf0211207] Strutt and Warrington, 2008, Development 135(18): 3103--3111 Planar polarity genes in the Drosophila wing regulate the localisation of the FH3-domain protein Multiple Wing Hairs to control the site of hair production. [FBrf0205732] Lee and Adler, 2004, Mech. Dev. 121(1): 37--49 The grainy head transcription factor is essential for the function of the frizzled pathway in the Drosophila wing. [FBrf0167844] Lee and Adler, 2002, Genetics 160(4): 1535--1547 The function of the frizzled pathway in the Drosophila wing is dependent on inturned and fuzzy. [FBrf0147064] Yun et al., 1999, Dev. Genet. 25(4): 297--305 The inturned protein of Drosophila melanogaster is a cytoplasmic protein located at the cell periphery in wing cells. [FBrf0112097] Adler et al., 1998, Development 125(5): 959--968 Mutations in the cadherin superfamily member gene dachsous cause a tissue polarity phenotype by altering frizzled signaling. [FBrf0101864] Park et al., 1996, Development 122(3): 961--969 The Drosophila tissue polarity gene inturned acts cell autonomously and encodes a novel protein. [FBrf0086571] Adler et al., 1994, Genetics 137(3): 829--836 The Drosophila tissue polarity gene inturned functions prior to wing hair morphogenesis in the regulation of hair polarity and number. [FBrf0072459] Krasnow and Adler, 1994, Development 120(7): 1883--1893 A single frizzled protein has a dual function in tissue polarity. [FBrf0073642]

Allele Dmel\inIH56

Allele Dmel\in^IH56