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General Information
Symbol
Dmel\HrsD28
Species
D. melanogaster
Name
FlyBase ID
FBal0039519
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
l(2)23AB5D28
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
point mutation
Nucleotide change:
C2741607T
Amino acid change:
Q156term | Hrs-PA; Q269term | Hrs-PB; Q269term | Hrs-PC
Reported amino acid change:
Q269term
Comment:
Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference
Amino acid replacement: Q270term.
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
Somatic clones of HrsD28/HrsD28 cells in the third instar larval eye disc display low levels of apoptosis (detected by Caspase-3 staining).
Loss of photoreceptors is seen in HrsD28/Df(2L)Exel6277 mutant eye discs.
HrsD28/HrsD28 MARCM ddaC clones show dendrite pruning similar to wild type at 18hr APF.
Homozygous mushroom body gamma neuron clones show normal axon pruning.
HrsD28 follicular epithelium clones display normal epithelial morphology. There is no detectable phenotype in HrsD28 mosaic eye imaginal discs or imaginal discs consisting predominantly of mutant cells. Eye discs consisting predominantly of cells mutant for HrsD28 progress to form adult eyes. These are smaller than wild-type and have a rough appearance but contain some mutant photoreceptors. The scarcity of mutant adult photoreceptors might be due to cell death, as apoptotic cells are seen these mutants.
Homozygotes die at the early pupal stage.
HrsD28 mosaic eye disc clones have a similar number of acidified endosomes to wild type.
Oocytes mutant for HrsD28 exhibit yolk granules containing intralumenal vesicles and isolated multivesicular bodies, although they are less abundant in the wild-type. In addition, internalised yolk proteins are detected in regularly shaped yolk granules.
HrsD28/Df(2L)Exel6277 eye discs show a loss of photoreceptor cells.
HrsD28 mutant cells are preferentially located at the front of the cluster during border cell posterior and dorsal migration in mosaic border cell clusters consisting of both wild-type and mutant cells.
HrsD28 clones within the wing discs contain enlarged late endosomes.
Only slight defects in border cell migration are apparent when border cells are part of HrsD28 homozygous somatic clones.
In mutant larval garland cells endosomes are dramatically enlarged. Wild-type endosomes mature by forming invaginations of their limiting membrane and eventually collapse upon themselves. In mutant garland cells there is a strong reduction the relative number of invaginated (5 fold) and collapsed (10 fold) endosomes. There is also a 5 fold reduction of small multivesicular bodies (MVBs) compared to wild-type.
Hemizygotes die as larvae.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Enhanced by
Enhancer of
Suppressor of
Statement
Reference
HrsD28 is a suppressor of phenotype of l(2)gd1d7
Hrs[+]/HrsD28 is a suppressor of eye disc phenotype of spin10403
Hrs[+]/HrsD28 is a suppressor of glial cell phenotype of spin10403
NOT Suppressor of
Statement
Reference
Other
Additional Comments
Genetic Interactions
Statement
Reference
Somatic clones of Rbf15aΔ;HrsD28 double homozygous cells display significantly higher levels of apoptosis in third instar larval wing and eye discs, even in clones posterior to the eye disc morphogenetic furrow, compared to clones homozygous for either Rbf15aΔ or HrsD28 alone. Differentiation of the R8 photoreceptor in HrsD28/HrsD28 somatic clones in the third instar larval eye disc is normal but differentiation of other photoreceptors is significantly delayed.
HrsD28 does not suppress the increased photoreceptor differentiation seen in shi7C7 mutant eye disc clones.
HrsD28/HrsD28 Stam2L2896/Stam2L2896 MARCM ddaC double mutant clones show dendrite pruning similar to wild type at 18hr APF.
HrsD28 Stam2L2896 double homozygous mushroom body gamma neuron clones show defects in axon pruning.
HrsD28 Stam2L2896 follicular epithelium clones display normal epithelial morphology. HrsD28 Stam2L2896 l(2)gl4 follicular epithelium clones display normal epithelial morphology. HrsD28 Stam2L2896 double mutant imaginal eye disc clonal cells form large clones of mesenchymal-like cells. Mosaic eye imaginal discs or eye imaginal discs consistently predominantly of cells mutant for both HrsD28 and Stam2L2896 show a loss of epithelial architecture phenotype. Eye discs consisting predominantly of cells mutant for HrsD28 Stam2L2896 progress to form adult eyes. These are smaller than wild-type and have a rough appearance but contain some mutant photoreceptors. The scarcity of mutant adult photoreceptors might be due to cell death, as apoptotic cells are seen these mutants.
The ectopic N activation phenotype (ectopic expression of Ecol\lacZDdc.be2.E(spl)m8-HLH) in l(2)gd1d7 wing disk clones is suppressed if cells are also mutant for HrsD28.
While HrsD28 homozygotes die at the early pupal stage, animals homozygous for HrsD28 and Df(1)rush4, sta+t4.4 never reach the pupal stage. Expression of rushScer\UAS.T:Avic\GFP under the control of Scer\GAL4arm.PS rescues this early lethality.
Average bouton number and average satellite bouton number at the neuromuscular junction is significantly increased compared to wild type in Snx16e01600 HrsD28/Snx16Δ1 Df(2L)Exel6277 double mutants.
The increased excitatory junctional current (EJC) amplitude seen at the neuromuscular junction in sky1/sky2 third instar larvae is suppressed if the larvae are also heterozygous for HrsD28.
HrsD28/+ suppresses the glial migration defects seen in spin10403 eye discs.
The ectopic upregulation of N signaling seen in l(2)gd1d7 clones is suppressed when clones also carry the HrsD28 mutation.
The external sensory organ multi-socket phenotype in l(2)gd124 animals is suppressed in a HrsD28 mutant background
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Rescued by
Comments
Images (0)
Mutant
Wild-type
Stocks (4)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (9)
References (43)