Somatic clones of HrsD28
cells in the third instar larval eye disc display low levels of apoptosis (detected by Caspase-3 staining).
MARCM ddaC clones show dendrite pruning similar to wild type at 18hr APF.
Homozygous mushroom body gamma neuron clones show normal axon pruning.
follicular epithelium clones display normal epithelial morphology.
There is no detectable phenotype in HrsD28
mosaic eye imaginal discs or imaginal discs consisting predominantly of mutant cells.
Eye discs consisting predominantly of cells mutant for HrsD28
progress to form adult eyes. These are smaller than wild-type and have a rough appearance but contain some mutant photoreceptors. The scarcity of mutant adult photoreceptors might be due to cell death, as apoptotic cells are seen these mutants.
Homozygotes die at the early pupal stage.
mosaic eye disc clones have a similar number of acidified endosomes to wild type.
Oocytes mutant for HrsD28
exhibit yolk granules containing intralumenal vesicles and isolated multivesicular bodies, although they are less abundant in the wild-type. In addition, internalised yolk proteins are detected in regularly shaped yolk granules.
mutant cells are preferentially located at the front of the cluster during border cell posterior and dorsal migration in mosaic border cell clusters consisting of both wild-type and mutant cells.
clones within the wing discs contain enlarged late endosomes.
Only slight defects in border cell migration are apparent when border cells are part of HrsD28
homozygous somatic clones.
In mutant larval garland cells endosomes are dramatically enlarged. Wild-type endosomes mature by forming invaginations of their limiting membrane and eventually collapse upon themselves. In mutant garland cells there is a strong reduction the relative number of invaginated (5 fold) and collapsed (10 fold) endosomes. There is also a 5 fold reduction of small multivesicular bodies (MVBs) compared to wild-type.
Hemizygotes die as larvae.