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General Information
Symbol
Dmel\aph-1D35
Species
D. melanogaster
Name
FlyBase ID
FBal0039520
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
point mutation
Nucleotide change:

C2766609T

Amino acid change:

R99term | aph-1-PA; R99term | aph-1-PB

Reported amino acid change:

R99term

Comment:

Site of nucleic acid difference inferred by FlyBase based on reported amino acid change.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

The mutation results in truncation of aph-1 protein between transmembrane domains 3 and 4.

Amino acid replacement: R99term.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Flies heterozygous for aph-1D35 have smaller eyes than wild-type flies.

Expression of aph-1Scer\UAS.T:SV5\V5 under the control of Scer\GAL4arm.PS partially rescues the lethality of aph-1D35.

Embryos from adult females bearing aph-1D35 germ-line clones lacking both maternal and zygotic aph-1 activity exhibit a N-like hyperplasia of the embryonic nervous system. Similarly, aph-1D35 mutant wing discs contain clusters of supernumerary sensory organ precursor (SOP) cells, in addition to an overall developmental arrest phenotype. aph-1D35/Df(2L)N6 embryos from adult females bearing aph-1D35 germ-line clones exhibit hyperplasia of the embryonic nervous system. Similarly, aph-1D35 mutant wing discs contain clusters of supernumerary sensory organ precursor (SOP) cells, in addition to an overall developmental arrest phenotype. aph-1D35 homozygous mutant tissue exhibit N-like phenotypes, including notches and missing margin structures in the wing, thoracic cuticle regions lacking microchaetae, and small eyes with disordered ommatidial packing. Homozygous aph-1D35 somatic clones, generated in heterozygous hosts exhibit phenotypes characteristic of impaired N function, including wing notching and bristle loss.

Hemizygotes cause larval or pupal lethality.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement
Reference

aph-1D35 has size defective | dominant phenotype, suppressible by crb11A22/crb[+]

Suppressor of
Statement
Reference
Phenotype Manifest In
Suppressed by
Statement
Reference

aph-1D35 has eye phenotype, suppressible by crb11A22/crb[+]

aph-1D35 has sensory mother cell & dorsal mesothoracic disc phenotype, suppressible by Nint.hs

NOT suppressed by
Statement
Reference

aph-1D35 has sensory mother cell & dorsal mesothoracic disc phenotype, non-suppressible by NECN.hs

NOT Enhancer of
Statement
Reference

aph-1[+]/aph-1D35 is a non-enhancer of phenotype of Psn9/Psn143

Suppressor of
Statement
Reference

aph-1D35 is a suppressor of phenotype of l(2)gd1d7

aph-1[+]/aph-1D35 is a suppressor of phenotype of l(2)gd1d7

NOT Suppressor of
Statement
Reference

aph-1[+]/aph-1D35 is a non-suppressor of phenotype of Psn9/Psn143

aph-1D35 is a non-suppressor of dorsal mesothoracic disc & sensory mother cell phenotype of Nint.hs

Additional Comments
Genetic Interactions
Statement
Reference

aph-1D35 strongly suppresses the overproliferation seen in lace2 mutant wing disc clones.

The ectopic N activation phenotype (ectopic expression of Ecol\lacZDdc.be2.E(spl)m8-HLH) in l(2)gd1d7 wing disk clones is suppressed if cells are also mutant for aph-1D35.

Heterozygosity for aph-1D35suppresses the ectopic N activation phenotype (ectopic expression of wg) in l(2)gd1d7 wing discs.

Heterozygous crb11A22 suppresses the aph-1D35/+ small eye phenotype.

NECN.hs mutants have no discernible inhibitory effect on sensory organ precursor cells in aph-1D35 wing discs. Nint.hs mutants exhibit nearly complete sensory organ precursor cell inhibition in aph-1D35 mutant wing discs.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Partially rescued by
Comments

Expression of aph-1Scer\UAS.T:SV5\V5 under the control of Scer\GAL4arm.PS partially rescues the lethality of aph-1D35.

Expression of aph-1Scer\UAS.T:SV5\V5 under the control of Scer\GAL4mat.αTub67C.T:Hsim\VP16 rescues the lethality of aph-1D35.

Images (0)
Mutant
Wild-type
Stocks (3)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
References (10)