FB2025_01 , released February 20, 2025
Allele: Dmel\orbF343
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General Information
Symbol
Dmel\orbF343
Species
D. melanogaster
Name
FlyBase ID
FBal0039708
Feature type
allele
Associated gene
Dmel\orb
Associated Insertion(s)
Carried in Construct
Also Known As
orb343
Key Links
Genomic Maps

Allele class

amorphic allele - genetic evidence

Mutagen
Nature of the Allele
Allele class

amorphic allele - genetic evidence

(Lantz et al., 1994)
Mutagen
ethyl methanesulfonate
(Christerson and McKearin, 1994)
Progenitor genotype
Cytology
Description

Amino acid replacement: Q240term.

(Rojas-Ríos et al., 2015)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
3R:23,269,124..23,269,124 [-]
Nucleotide change:

C23269124T

Amino acid change:

Q240term | orb-PA; Q238term | orb-PB; Q238term | orb-PD; Q240term | orb-PG

Reported amino acid change:

Q240term

Comment:

Site and nature of nucleotide substitution in mutant inferred by FlyBase curator based on reported amino acid change.

(Rojas-Ríos et al., 2015)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      ameliorates  oculopharyngeal muscular dystrophy
      modeled by Hsap\PABPN117ala.Act88F
      (Chartier et al., 2015)
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      5-10% of the eggs laid by females heterozygous for orbF343 have ventralised chorions.

      When a single copy of orb8E2Hd.Hsp83.T:Ecol\lacZ is introduced into orbF343/+ females, orb8E2Hd.Hsp83.T:Ecol\lacZ increases the frequency of ventralised eggs. orb8E2Hd.Hsp83.T:Ecol\lacZ rin3/+ females lay between 3-30% ventralised eggs depending on the temperature.

      (Costa et al., 2013)

      Almost 30% of eggs laid by orb8E2Hd.Hsp83.T:Ecol\lacZ orbF343/+ females have ventralised chorions.

      (Wong et al., 2011)

      More than half of the eggs laid at 18°C by females heterozygous for orbF343 have ventralized egg shells.

      (Costa et al., 2005)

      85% of embryos derived from heterozygous female hatch.

      (Castagnetti and Ephrussi, 2003)

      100% of eggs laid by orb7D4-11/orbF343 or orbmel/orbF343 females are ventralised and unfertilised. 90% of eggs laid by orb7D1-16/orbF343 females are ventralised and unfertilised. The remaining eggs give rise to embryos with abdominal defects. Egg chambers of orb9D6-3/orbF343 females arrest early during oogenesis and fail to reach vitellogenic stages. 90% of egg chambers of orb7E4-5/orbF343 females arrest early during oogenesis and fail to reach vitellogenic stages. Eggs that are laid are ventralised and unfertilised.

      (Martin et al., 2003)

      0% of eggs derived from orbmel/orbF343 females have wild-type dorsal appendages, 3.4% have fused dorsal appendages and 96.6% have no dorsal appendages. The eggs do not hatch.

      (Masrouha et al., 2003)

      16-cell cysts do not form in orbF343 ovaries.

      (Manheim et al., 2002)

      10-15% of eggs produced by females carrying orbF343 have ventralised chorions. 23-45% of eggs produced by orbF343/+ females carrying one copy of orbNH.Hsp83.T:Ecol\lacZ have ventralised chorions (depending on the orbNH.Hsp83.T:Ecol\lacZ line used). 27.1% of eggs produced by orbF343/+ females carrying one copy of orb8E2Hd.Hsp83.T:Ecol\lacZ have ventralised chorions. 74.6% of eggs produced by orbF343/+ females carrying two copies of orb8E2Hd.Hsp83.T:Ecol\lacZ have ventralised chorions. 42.6% of eggs produced by orbF343/+ females carrying one copy of orbAN.Hsp83.T:Ecol\lacZ have ventralised chorions. 22.0% of eggs produced by orbF343/+ females carrying one copy of orbAE.Hsp83.T:Ecol\lacZ have ventralised chorions.

      (Tan et al., 2001)

      The female germline cyst fails to go through the last mitotic division and contains only eight cells. All of the cells in region 2a seem to form a complete synaptonemal complex (SC). Most mutant cysts degenerate in region 2, but clusters of cells are occasionally seen in region 3, which are presumably single cysts that have not degenerated, as they always contain 8 cells. In these cases, all 8 nuclei show strong SC staining reminiscent of the pachytene stage.

      (Huynh and St. Johnston, 2000)

      Oogenesis is arrested at or just before the formation of the 16-cell cyst in mutant ovaries.

      (Chang et al., 1999)

      Degenerating cysts. Some heterozygous orbmel/orbF343 embryos display absence of dorsal appendages, oocytes are small than normal and surrounded by columnar follicle cells and eggs are also smaller than normal. orbF343 interacts genetically with Egfr and grk, double heterozygote embryos display fused dorsal appendages.

      (Christerson and McKearin, 1994)

      Oogenesis has blocked during a stage of cyst development, the ovaries are very small and consist of germarium like structures populated by many small undifferentiated cells.

      (Lantz et al., 1994)

      orbmel/orbF343 causes lateralization. Eggs are shortened and rounded posteriorly like dorsalized eggs but like ventralized eggs their dorsal appendages are fused dorsally, in 49% of eggs, or absent, in 46% of the eggs. 37% of the eggs contain embryos.

      (Roth and Schupbach, 1994)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressed by
      NOT suppressed by
      Statement
      Reference

      orbF343 has female sterile | recessive phenotype, non-suppressible by ypsJM2

      (Mansfield et al., 2002)
      Suppressor of
      Statement
      Reference

      orbF343/orb[+] is a suppressor | partially of abnormal cell number | oogenesis phenotype of HecwCI

      (Fajner et al., 2021)

      orbF343/orb[+] is a suppressor | partially of abnormal cell number | oogenesis phenotype of HecwKO

      (Fajner et al., 2021)

      orbF343/orb[+] is a suppressor of visible phenotype of Hsap\PABPN117ala.Act88F

      (Chartier et al., 2015)
      Phenotype Manifest In
      Enhanced by
      NOT Enhanced by
      Statement
      Reference

      Ecol\lacZHsp83.orb.8E2Hd, orbF343 has egg chorion | maternal effect phenotype, non-enhanceable | maternal effect by CkIIαTikR/CkIIalpha[+]

      (Wong et al., 2011)
      Suppressed by
      Statement
      Reference

      orbF343/orbmel has embryo phenotype, suppressible | partially by PlpWT.Ubi.GFP

      (Fang and Lerit, 2022)

      Ecol\lacZHsp83.orb.8E2Hd, orbF343 has egg chorion | maternal effect phenotype, suppressible | heat sensitive | maternal effect by Fmr13/Fmr1[+]

      (Costa et al., 2005)

      orbF343 has egg chorion | maternal effect phenotype, suppressible by Fmr13/Fmr1[+]

      (Costa et al., 2005)

      orbF343 has egg chorion | maternal effect phenotype, suppressible by Fmr13/Fmr13

      (Costa et al., 2005)
      Enhancer of
      Statement
      Reference

      orbF343, orb[+], Ecol\lacZHsp83.orb.8E2Hd is an enhancer of egg chorion | maternal effect phenotype of CkIIαTik

      (Wong et al., 2011)
      Suppressor of
      Statement
      Reference

      orbF343/orb[+] is a suppressor | partially of oocyte | ectopic phenotype of HecwKO

      (Fajner et al., 2021)

      orbF343/orb[+] is a suppressor | partially of egg chamber phenotype of HecwKO

      (Fajner et al., 2021)

      orbF343/orb[+] is a suppressor | partially of female germline cell phenotype of HecwCI

      (Fajner et al., 2021)

      orbF343/orb[+] is a suppressor | partially of egg chamber phenotype of HecwCI

      (Fajner et al., 2021)

      orbF343/orb[+] is a suppressor | partially of oocyte | ectopic phenotype of HecwCI

      (Fajner et al., 2021)

      orbF343/orb[+] is a suppressor | partially of female germline cell phenotype of HecwKO

      (Fajner et al., 2021)

      orbF343/orb[+] is a suppressor of wing phenotype of Hsap\PABPN117ala.Act88F

      (Chartier et al., 2015)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The frequency of ventralised eggs is increased three-fold or more depending on the temperature when orb8E2Hd.Hsp83.T:Ecol\lacZ rin3 orbF343/+ mothers are also heterozygous for rin3.

      (Costa et al., 2013)

      Over 60% of eggs laid by CkIIαTik/orb8E2Hd.Hsp83.T:Ecol\lacZ orbF343 females have ventralised chorions.

      CkIIαTikR/+ does not increase the frequency of the ventralised chorion phenotype seen in eggs laid by orb8E2Hd.Hsp83.T:Ecol\lacZ orbF343/+ females.

      CkIIβmbuΔA26-2L/+ increases the frequency of the ventralised chorion phenotype seen in eggs laid by orb8E2Hd.Hsp83.T:Ecol\lacZ orbF343/+ females to 75%.

      (Wong et al., 2011)

      The frequency of ventralized eggs shells drops from >50% in eggs laid by orbF343/+ females to 15% in eggs produced by orbF343 /+ Fmr13/+ females and to less than 1% in those laid by orbF343 /+ Fmr13/Fmr13.

      The D-V polarity defects associated with orb8E2Hd.Hsp83.T:Ecol\lacZ (P{8E2Hd}19G) orbF343/+ females are partially suppressed by heterozygosity for Fmr13 at 18°C and completely suppressed at 25°C and 29°C.

      The presence of Fmr1+t14 in a orb8E2Hd.Hsp83.T:Ecol\lacZ (P{8E2Hd}19G); orbF343/+ background results in a significant enhancement in the number of ventralized eggs laid at 25°C and 29°C.

      (Costa et al., 2005)

      87% of embryos derived from BicC5/+ ; orbF343/+ females hatch.

      (Castagnetti and Ephrussi, 2003)

      The dorsal appendage defects of eggs derived from orbmel/orbF343 females are not rescued by Df(2L)be408 P{CG10728+tMa}/Df(2L)pr2b P{barr+tMa}.

      (Masrouha et al., 2003)

      The failure of eggs derived from orbmel/orbF343 females to hatch is very weakly suppressed by ypsJM2/ypsJM2; rare escapers complete embryogenesis and hatch.

      (Mansfield et al., 2002)
      Xenogenetic Interactions
      Statement
      Reference

      orbF343 suppresses the wing posture defects seen upon expression of Hsap\PABPN117ala.Act88F.

      (Chartier et al., 2015)
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Discoverer

      C. Nusslein-Volhard.

      (Lantz et al., 1994)
      Comments
      Comments

      Class I orb allele.

      (Lantz et al., 1994)

      Mutation does not affect posterior follicle cell determination.

      (Gonzalez-Reyes et al., 1995)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      References (28)