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General Information
Symbol
Dmel\pbhs.PB
Species
D. melanogaster
Name
heat shock construct of Boube
FlyBase ID
FBal0039734
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

Hsp70 promoter governing expression of pb minigene. Includes all the pb coding region apart from the 15bp alternatively spliced microexon.

Allele components
Product class / Tool use(s)
Regulatory region(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

The induction of pbhs.PB occasionally exhibit an ectopic distal sex comb on the T1 leg.

Flies expressing pbhs.PB show a progressive transformation of antennae to maxillary palps as the copy number of P{HSPB} elements is increased. The transformation does not affect the most proximal antennal tissues, and acts differentially along the proximo-distal axis, from proximal toward distal. Flies carrying two copies of pbhs.PB show loss of many distal, but not proximal, wing margin mechanosensory bristles. This phenotype is enhanced by HDMu4.

Flies carrying pbhs.PB show a fully penetrant, dose-sensitive dominant transformation of antennae towards maxillary palps in the absence of heat shock.

Uninduced expression causes antenna to maxillary palps transformation. Subtle dose-sensitive defects are also observed in the eyes, posterior head, prothoracic legs and wings. Two copies of the insertion cause reduction or loss of the ventral aspect of the adult eye, a frequent change in ommatidial geometry, from hexagonal to tetragonal, in remaining ommatidia and duplication of some interommatidial bristles. Hemizygous males have normal eyes.

Flies expressing a single copy of pbhs.PB at a basal level (in the absence of heat shock) show a partial transformation of antennae to maxillary palps. Approximately 10% of females and 2% of males have an ectopic bristle on the distal end of wing vein L3. Flies carrying two copies of pbhs.PB show a nearly complete antenna to maxillary transformation.

Heat induced expression does not affect tarsal leg structures but causes some thickening of the arista.

Dose sensitive dominant transformation of antenna towards maxillary palps with 100% penetrance, this phenotype can be induced in the absence of heat shock.

Uninduced expression of pbhs.PB causes a novel, fully penetrant dominant transformation of antennal segment 3 and the aristae towards maxillary palps. Antennal segment 2 is perturbed but not explicitly transformed. Transformation of the arista can be augmented by altering the culture temperature from 22oC to 29oC. In general flies exhibit reduced viability and mobility. Dose sensitive effects were noticed for prothoracic legs (ectopic and perturbed sex combs), head capsule (malformed outer vertical bristles, reduction of the anterior aspect of the eye) and wings (loss or alteration of the anterior wing margin bristles, and formation of microchaetae at the distal extremity of wing vein L3). Overexpression by heat induction does not generate marked increase in adult cuticular phenotypes.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference

pbhs.PB has visible phenotype, enhanceable by RasGAP1sxt-BJ61

Suppressed by
Statement
Reference

RasGAP1sxt-BJ61, pbhs.PB/pb[+] has visible phenotype, suppressible by Ras85De1B/Ras85D[+]

RasGAP1sxt-BJ61, pbhs.PB has visible phenotype, suppressible by Ras85De1B/Ras85D[+]

Enhancer of
Statement
Reference

pbhs.PB is an enhancer of visible phenotype of RasGAP1sxt-BJ61

Other
Phenotype Manifest In
Enhanced by
Statement
Reference

pbhs.PB has prothoracic leg | distal phenotype, enhanceable by skdpap-53

pbhs.PB has sex comb | ectopic phenotype, enhanceable by skdpap-53

pbhs.PB has wing sensillum phenotype, enhanceable by HDMu4

pbhs.PB has antenna phenotype, enhanceable by RasGAP1sxt-BJ61

Suppressed by
Statement
Reference

pbhs.PB has prothoracic leg | distal phenotype, suppressible by Scr4

pbhs.PB has sex comb | ectopic phenotype, suppressible by Scr4

pbhs.PB, skdpap-53 has prothoracic leg | distal phenotype, suppressible by Scr4

pbhs.PB, skdpap-53 has sex comb | ectopic phenotype, suppressible by Scr4

RasGAP1sxt-BJ61, pbhs.PB/pb[+] has antenna phenotype, suppressible by Ras85De1B/Ras85D[+]

RasGAP1sxt-BJ61, pbhs.PB/pb[+] has wing vein phenotype, suppressible by Ras85De1B/Ras85D[+]

RasGAP1sxt-BJ61, pbhs.PB has antenna phenotype, suppressible by Ras85De1B/Ras85D[+]

RasGAP1sxt-BJ61, pbhs.PB has wing vein phenotype, suppressible by Ras85De1B/Ras85D[+]

Enhancer of
Statement
Reference

pbhs.PB is an enhancer of wing vein phenotype of RasGAP1sxt-BJ61

Other
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

60% of flies carrying pbhs.PB that are also heterozygous for taraL4 show partial truncation of the distal part of wing vein L4. This phenotype is not seen in either pbhs.PB single mutant or taraL4/+ single mutant flies. Deletions of wing vein L5 are also seen in the double mutant flies.

The addition of Scr4 suppresses and the addition of skdpap-53 enhances the ectopic distal sex comb phenotype. The addition of Scr4 to pbhs.PB, skdpap-53 flies also suppresses the distal sex comb phenotype.

pbhs.PB HE31/Ras85De1F triple heterozygotes have deletions of the distal portions of wing veins L4 and L5, up to and including the posterior crossvein. The spacing and position of the campaniform sensilla along the proximo-distal axis of wing vein L3 is altered.

Shows a dose-sensitive interaction with Df(2L)net-PMF, Df(2L)30A-C, Df(2L)TW50, Df(2R)en30, Df(2R)PC4, Df(3L)GN24, Df(3L)AC1, Df(3R)by10, Df(3R)by416, Df(3R)GB104, Df(3R)P115 and Df(3R)L127. Does not interact with Df(3R)by62. Df(3R)by10, Df(3R)by416 and Df(3R)GB104 reduce the antennal to maxillary transformation caused by pbhs.PB. pbhs.PB Ras85De1B double heterozygotes have a partially penetrant truncation of wing veins L4 and L5, and the frequency of ectopic bristle formation on the distal end of wing vein L3 (seen in pbhs.PB heterozygotes) is increased to 90% for females and 50% for males. pbhs.PB Df(3L)AC1 double heterozygotes show a very weak modification of the pbhs.PB wing phenotype. Gap1sxt-BJ61 enhances the antenna to maxillary transformation in pbhs.PB/+ Gap1sxt-BJ61/Gap1sxt-BJ61 flies. This enhancement is reduced if the flies also carry one copy of Ras85De1B. Enhances the ectopic wing vein phenotype seen in Gap1sxt-BJ61 homozygotes. This enhancement is reduced if the flies also carry one copy of Ras85De1B.

Induction of expression in ssa40aw flies causes shortening of the distal parts of the tarsus, mostly in the antennae, legs are less affected.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Partially rescues

pbhs.PB partially rescues pb5

Comments

Rescues the phenotype of recessive pb mutations.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
Comments
Comments

Elevated temperatures supplement the level of expression of this allele. Attempts to generate a fly containing three copies have been unsuccessful.

Flies carrying pbhs.PB have been used in a genetic screen for dominant modifiers of pb function.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
pbhs.PB
Name Synonyms
heat shock construct of Boube
Secondary FlyBase IDs
    References (11)