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General Information
Symbol
Dmel\RafF22.hs
Species
D. melanogaster
Name
FlyBase ID
FBal0039752
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
hsRaf*M7
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

An Hsp70 promoter drives expression of the Raf coding sequence in which amino acid residues 2-431 have been deleted.

Allele components
Product class / Tool use(s)
Regulatory region(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

phlF22.hs heterozygous flies maintained at 25[o]C exhibit normal life spans and locomotor activity.

Overexpression of phlF22.hs does not affect superoxide production.

16.6% of eggs derived from females expressing one copy of phlF22.hs using heat shock are dorsalised, with dorsal appendage material being expanded throughout the anterior of the egg. The penetrance of the dorsalised egg phenotype is 61.7% if the females express two copies of phlF22.hs.

Expression of phlF22.hs during the late third larval instar stage using heat shock results in flies that show a significant amount of wing vein loss. Heat shock during the early-to-mid second larval instar stages results in flies with ectopic wing vein tissue, in most cases in close proximity to a vein.

Ubiquitous expression in the embryo weakly suppresses normal cell death.

Modulation of voltage gated K+ currents induced by the neuropeptide pituitary adenylyl cyclase-activating polypeptide (PACAP38) is eliminated. The mutation fails to block or mimic PACAP38 (neuropeptide pituitary adenylyl cyclase-activating polypeptide) induced enhancement of K+ currents.

Expression of an activated phl mutation has no effect on size of Nf1 mutant pupae.

After 3 days of heat shock homozygous females lay dorsalised embryos, dorsal appendages are fused in a ring at the anterior end and eggs are smaller and more spherical than wild type (comparable to eggs laid by fs(1)K101 females). Some embryos exhibit a more severe phenotype. Expression of Hsap\RAF1gof.hs leads to more embryos exhibiting the stronger chorion phenotype.

Females lay eggs with a dorsalised chorion.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT Suppressor of
Statement
Reference

RafF22.hs is a non-suppressor of abnormal locomotor rhythm phenotype of Nf1P1

Phenotype Manifest In
Enhanced by
Statement
Reference

RafF22.hs has dorsal appendage phenotype, enhanceable by dodhs.PH

Suppressed by
Statement
Reference

RafF22.hs has egg chorion phenotype, suppressible by Dsor1+t9

Additional Comments
Genetic Interactions
Statement
Reference

The penetrance of the dorsalised egg phenotype produced by females expressing one copy of phlF22.hs is enhanced (to 56.8%) if the females are also expressing dodhs.PH.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer

A.H. Brand and N. Perrimon.

Comments
Comments

Constitutively active form of phl.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (6)
Reported As
Symbol Synonym
RafF22.hs
hsΔDraf22
phlF22.hs
Name Synonyms
Secondary FlyBase IDs
    References (14)