FB2025_01 , released February 20, 2025
Allele: Dmel\RafUAS.F179
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General Information
Symbol
Dmel\RafUAS.F179
Species
D. melanogaster
Name
FlyBase ID
FBal0039757
Feature type
allele
Associated gene
Dmel\Raf
Associated Insertion(s)
Carried in Construct
P{UAS-Raf.gof}
Also Known As
UAS-Rafgof, UAS-rafF179, RafGOF, UAS-RafAct, UAS-DrafGOF, UAS-Raf, UAS rafGOF, UAS-phl.gof, UAS-Raf.gof, UAS-Raf-GOF
Key Links
Transgenic product class
inframe_deletion
(Brand and Perrimon, 1994)
Mutagen
Nature of the Allele
Transgenic product class
inframe_deletion
(Brand and Perrimon, 1994)
Mutagen
in vitro construct
(Brand, 1997.6.30, Stemerdink and Jacobs, 1997, Brand and Perrimon, 1994)
Progenitor genotype
Carried in construct
P{UAS-Raf.gof}
Cytology
Description

A constitutively active form of Raf is expressed under the control of UAS regulatory sequences.

(Stemerdink and Jacobs, 1997)

UAS regulatory sequences drive expression of Raf protein in which amino acids 2-431 are deleted.

(Brand and Perrimon, 1994)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Raf
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      model of  cancer
      is ameliorated by Atg1UAS.cSa
      (Lee et al., 2021)
      is ameliorated by dindRNAi.UAS.RNAi
      (Ren et al., 2022)
      exacerbates  gastrointestinal system cancer
      modeled by Apc2g10, ApcQ8
      (Wang et al., 2013)
      exacerbates  carcinoma
      modeled by ftk07918
      (Garoia et al., 2005)
      modeled by ftG-rv
      (Garoia et al., 2005)
      model of  carcinoma
      is ameliorated by hepAct.UAS
      (Uhlirova et al., 2005)
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class

      abnormal cell growth | larval stage | somatic clone, with Scer\GAL4Act5C.PI

      (Uhlirova et al., 2005)

      abnormal cell number | adult stage, with Scer\GAL4esg.PU

      (Shi et al., 2023)

      abnormal cell polarity | conditional | somatic clone, with Scer\GAL4FRT.Act5C, Scer\GAL80αTub84B.PL

      (Zeng et al., 2010)

      abnormal neuroanatomy, with Scer\GAL4futsch-C380

      (Koh et al., 2002)

      decreased body size | adult stage, with Scer\GAL4Ras64B.PW

      (Walker et al., 2006)

      hyperplasia | conditional | somatic clone, with Scer\GAL4FRT.Act5C, Scer\GAL80αTub84B.PL

      (Zeng et al., 2010)

      increased cell number | adult stage, with Scer\GAL4Delta-05151-G

      (Ren et al., 2022)

      increased cell number | adult stage, with Scer\GAL4esg.PU

      (Ren et al., 2022, Zhao et al., 2022)

      increased cell number | adult stage, with Scer\GAL4sev.PU

      (Kim et al., 2004)

      increased cell number | adult stage | somatic clone, with Scer\GAL4Act5C.PI

      (Zhao et al., 2022)

      increased cell number | adult stage | somatic clone, with Scer\GAL4Act.PU

      (Ren et al., 2022)

      increased cell number | larval stage | somatic clone, with Scer\GAL4Act5C.PI

      (Uhlirova et al., 2005)

      increased cell number | third instar larval stage, with Scer\GAL4NP7397

      (Jiang and Edgar, 2009)

      increased cell size | somatic clone, with Scer\GAL4Act5C.PP

      (Prober and Edgar, 2002)

      increased occurrence of cell division | adult stage, with Scer\GAL4esg.PU

      (Shi et al., 2023, Ren et al., 2022, Zhao et al., 2022)

      increased size | spermatogenesis, with Scer\GAL4VP16.bam

      (Azuma et al., 2021)

      lethal, with Scer\GAL471B

      (Reich et al., 1999)

      lethal - all die before end of P-stage | somatic clone, with Scer\GAL4Act5C.PI

      (Uhlirova et al., 2005)

      lethal - all die during embryonic stage | maternal effect, with Scer\GAL455B

      (Brand and Perrimon, 1994)

      neoplasia | adult stage, with Scer\GAL4Delta-05151-G

      (Ren et al., 2022)

      neoplasia | pharate adult stage | somatic clone, with Scer\GAL4Act5C.PI

      (Uhlirova et al., 2005)

      some die during embryonic stage | maternal effect, with Scer\GAL455B

      (Brand and Perrimon, 1994)

      some die during pharate adult stage | somatic clone, with Scer\GAL4Act5C.PI

      (Uhlirova et al., 2005)

      visible, with Scer\GAL4bbg-C96

      (Herr et al., 2010)

      visible, with Scer\GAL4GMR.PU

      (Grillo-Hill et al., 2015, Yan et al., 2010)

      visible, with Scer\GAL4hs.2sev

      (Kim et al., 2006)

      visible, with Scer\GAL4sca-537.4

      (Escudero et al., 2003)

      visible | adult stage, with Scer\GAL4sev.PU

      (Kim et al., 2004)
      Phenotype Manifest In

      adult Malpighian tubule | conditional | somatic clone, with Scer\GAL4FRT.Act5C, Scer\GAL80αTub84B.PL

      (Zeng et al., 2010)

      adult midgut, with Scer\GAL4esg.PU

      (Shi et al., 2023)

      adult midgut progenitor cell | third instar larval stage, with Scer\GAL4NP7397

      (Jiang and Edgar, 2009)

      adult posterior midgut epithelium, with Scer\GAL4esg.PU

      (Ren et al., 2022, Zhao et al., 2022)

      adult posterior midgut epithelium | somatic clone, with Scer\GAL4Act5C.PI

      (Zhao et al., 2022)

      adult posterior midgut epithelium | somatic clone, with Scer\GAL4Act.PU

      (Ren et al., 2022)

      bouton | increased number, with Scer\GAL4futsch-C380

      (Koh et al., 2002)

      cone cell | pupal stage | increased number, with Scer\GAL4GMR.PU

      (Grillo-Hill et al., 2015)

      dorsal appendage, with Scer\GAL4c532

      (Deng and Bownes, 1997)

      dorsal appendage | maternal effect, with Scer\GAL455B

      (Brand and Perrimon, 1994)

      egg chorion | maternal effect, with Scer\GAL455B

      (Brand and Perrimon, 1994)

      embryo | maternal effect, with Scer\GAL455B

      (Brand and Perrimon, 1994)

      embryonic/larval hemocyte | increased number, with Scer\GAL4Cg.PA

      (Asha et al., 2003)

      embryonic/larval tracheal section, with Scer\GAL4btl.PS

      (Iordanou et al., 2014)

      eye, with Scer\GAL4GMR.PU

      (Grillo-Hill et al., 2015, Yan et al., 2010)

      eye, with Scer\GAL4sev.PU

      (Kim et al., 2004)

      eye | somatic clone, with Scer\GAL4Act5C.PI

      (Uhlirova et al., 2005)

      eye-antennal disc | larval stage, with Scer\GAL4dpp.PU

      (Snee et al., 2016)

      eye-antennal disc | somatic clone, with Scer\GAL4Act5C.PI

      (Uhlirova et al., 2005)

      eye-antennal disc | somatic clone | third instar larval stage, with Scer\GAL4Tub.PU

      (Khoo et al., 2013)

      eye disc, with Scer\GAL4GMR.PU

      (Grillo-Hill et al., 2015)

      eye photoreceptor cell | adult stage | increased number, with Scer\GAL4sev.PU

      (Kim et al., 2004)

      eye photoreceptor cell | ectopic, with Scer\GAL4da.G32

      (Roignant et al., 2006)

      eye photoreceptor cell | somatic clone | third instar larval stage, with Scer\GAL4Tub.PU

      (Khoo et al., 2013)

      follicle cell | maternal effect, with Scer\GAL455B

      (Brand and Perrimon, 1994)

      interommatidial bristle, with Scer\GAL4hs.2sev

      (Kim et al., 2006)

      intestinal stem cell | adult stage | increased number, with Scer\GAL4esg.PU

      (Shi et al., 2023)

      intestinal stem cell of posterior adult midgut epithelium | increased number, with Scer\GAL4Delta-05151-G

      (Ren et al., 2022)

      intestinal stem cell of posterior adult midgut epithelium | increased number, with Scer\GAL4esg.PU

      (Zhao et al., 2022)

      macrochaeta, with Scer\GAL4sca-537.4

      (Escudero et al., 2003)

      mesothoracic tergum & macrochaeta | ectopic, with Scer\GAL4sca-C253

      (Hasson et al., 2005)

      midline glial cell | ectopic, with Scer\GAL4sli.PS

      (Scholz et al., 1997, Scholz et al., 1997)

      mitotic cell cycle | somatic clone, with Scer\GAL4Act5C.PP

      (Prober and Edgar, 2002)

      ommatidium, with Scer\GAL4hs.2sev

      (Kim et al., 2006)

      ommatidium | adult stage, with Scer\GAL4sev.PU

      (Kim et al., 2004)

      retina | adult stage, with Scer\GAL4GMR.PU

      (Grillo-Hill et al., 2015)

      retina | pupal stage, with Scer\GAL4GMR.PU

      (Grillo-Hill et al., 2015)

      rhabdomere | adult stage, with Scer\GAL4GMR.PU

      (Grillo-Hill et al., 2015)

      rhabdomere | increased number, with Scer\GAL4hs.2sev

      (Kim et al., 2006)

      secondary pigment cell | pupal stage, with Scer\GAL4GMR.PU

      (Grillo-Hill et al., 2015)

      spermatocyte, with Scer\GAL4VP16.bam

      (Azuma et al., 2021)

      wing cell, with Scer\GAL4hs.PB

      (Yan et al., 2009)

      wing disc | larval stage, with Scer\GAL4Bx-MS1096

      (Snee et al., 2016)

      wing disc | larval stage, with Scer\GAL4dpp.PU

      (Snee et al., 2016)

      wing margin, with Scer\GAL4bbg-C96

      (Herr et al., 2010)

      wing vein, with Scer\GAL4bbg-C96

      (Herr et al., 2010)

      wing vein, with Scer\GAL4hs.PB

      (Yan et al., 2009)
      Detailed Description
      Statement
      Reference

      Adulthood-only expression of RafUAS.F179 under the control of Scer\GAL4esg.PU leads to an increase in the number of stem cells and increase in mitotic index in the gut.

      Adulthood-induced intestinal clones that express RafUAS.F179 under the control of Scer\GAL4Act5C.PI are larger than control clones.

      (Zhao et al., 2022)

      Expression of RafScer\UAS.F179 driven by Scer\GAL4dpp.PU leads to tissue overgrowth in eye-antennal imaginal or wing discs (also in wing discs when driven by Scer\GAL4Bx-MS1096).

      (Snee et al., 2016)

      The external surface of eyes expressing phlScer\UAS.F179 under the control of Scer\GAL4GMR.PU is disorganised with indistinct facets and a rough exterior retinal surface. Some ommatidia show thin or absent lenses. Rhabdomeres are misoriented and occasionally missing. The rhabdomeres are distinct from wild type appearing more rectangular, especially in R1, R3, R5 and R6.

      In third instar larvae, expression of phlScer\UAS.F179 leads to disrupted eye disc morphology, with irregular ommatidial spacing and folding of the disc. Neuronal cell fates appear unaffected. By the pupal stages, cell morphologies are profoundly aberrant, with extra and misshapen cone cells and ommatidia misaligned along the D/V axis. Immature cone cell contacts are maintained and secondary pigment cells are absent between some pairs of neighbouring ommatidia.

      The intracellular pH (pHi) in eye discs expressing phlScer\UAS.F179 under the control of Scer\GAL4GMR.PU is significantly higher than in controls. The pHi remains higher in pupal eyes.

      (Grillo-Hill et al., 2015)

      Scer\GAL4btl.PS-mediated expression of phlScer\UAS.F179 causes extra tracheal branching.

      (Iordanou et al., 2014)

      Eye-antennal disc clones expressing RafScer\UAS.F179 under the control of Scer\GAL4tub.PU exhibit ectopic differentiation of photoreceptors in and interior to the morphogenetic furrow.

      (Khoo et al., 2013)

      Scer\GAL4bbg-C96-driven phlScer\UAS.F179 expression results in wing notching and truncation of the veins.

      (Herr et al., 2010)

      Expression of phlScer\UAS.F179 in differentiating eye cells driven by Scer\GAL4GMR.PU causes rough eyes of reduced size.

      (Yan et al., 2010)

      Expression of phlScer\UAS.F179 in adult Malpighian tubule clones (using the MARCM system, under the control of Scer\GAL80αTub84B.PL and Scer\GAL4Scer\FRT.Act5C) results in renal and nephric stem cell over proliferation, and results in tumorous growth.

      (Zeng et al., 2010)

      Expression of phlScer\UAS.F179 driven by Scer\GAL4esg-NP7397 in adult midgut progenitor cells results in their overproliferation compared with control third instar larval midguts.

      (Jiang and Edgar, 2009)

      Expression of phlScer\UAS.F179 in mushroom bodies under the control of Scer\GAL4c747 does not impair or enhance learning.

      (Moressis et al., 2009)

      Expression of phlScer\UAS.F179 after a 30 minute heat-shock at 37[o]C, under the control of Scer\GAL4hs.PB, results in the formation of ectopic vein cells.

      (Yan et al., 2009)

      Cells expressing phlScer\UAS.F179 under the control of Scer\GAL4c522 are preferentially located at the front of the cluster during border cell posterior and dorsal migration in mosaic border cell clusters consisting of both wild-type and mutant cells.

      (Bianco et al., 2007)

      Expression of phlScer\UAS.F179, under the control of Scer\GAL4hs.2sev, causes a roughened eye phenotype with extra rhabdomeres in some ommatidia.

      (Kim et al., 2006)

      Expression of phlScer\UAS.F179, under the control of Scer\GAL4da.G32, causes ectopic eye photoreceptor cell differentiation.

      (Roignant et al., 2006)

      Expression of phlScer\UAS.F179 under the control of Scer\GAL4Ras64B.PW results in adult flies that are smaller than normal at 16</up>oC.

      (Walker et al., 2006)

      When phlScer\UAS.F179 is driven by Scer\GAL4dpp.blk1 no effect is seen on wing disc growth.

      (Garoia et al., 2005)

      Expression of phlScer\UAS.F179, driven by Scer\GAL4sca-C253, leads to expression of ectopic bristles on the notum.

      (Hasson et al., 2005)

      Clones of eye imaginal discs that express phlScer\UAS.F179 autonomously (under the control of Scer\GAL4Act5C.PI) develop into vigorously proliferating tissue during larval stages and ultimately overgrow much of the adult eye. The overgrowth is not invasive and the animals survive until pharate adult stages, with rare adult escapers.

      (Uhlirova et al., 2005)

      Expression of phlScer\UAS.F179 under the control of Scer\GAL4Cg.PA results in a massive increase in hemocyte number in larvae compared to wild type.

      (Asha et al., 2003)

      Expression of phlScer\UAS.F179, under the control of Scer\GAL4C380 results in a dramatic increase in bouton number, comparable to that found in Ras85DV12.Scer\UAS or Ras85DV12.S35.Scer\UAS mutants.

      (Koh et al., 2002)

      Expression of phlScer\UAS.F179 under the control of Scer\GAL4Act5C.PP in wing disc cells results in an increase in cell size and an increase in clone area compared to controls. The proportion of cells in G1 is decreased compared to controls. Clones expressing phlScer\UAS.F179 under the control of Scer\GAL4Act5C.PP are significantly more round than control clones.

      (Prober and Edgar, 2002)

      Expression of phlScer\UAS.F179 under the control of Scer\GAL4slbo.2.6 has no effect on border cell migration.

      (Duchek and Rorth, 2001)

      The ventralised phenotype of eggs derived from females expressing kek1Scer\UAS.cGa under the control of Scer\GAL4T155 is overridden if the females are also coexpressing phlScer\UAS.F179; the resulting eggs are dorsalised.

      (Ghiglione et al., 1999)

      Scer\GAL4c532-mediated expression causes 90% of mature eggs to have no dorsal appendages.

      (Deng and Bownes, 1997)

      Scer\GAL4sli.PS-mediated expression causes ectopic midline glial cells.

      (Scholz et al., 1997, Scholz et al., 1997)

      Scer\GAL4 activation of phl in the follicle cells is sufficient to dorsalise the egg. Of the fertilised eggs only 25% give rise to dorsalised embryos, in the severe cases embryos are twisted and lack nearly all ventral structures.

      (Brand and Perrimon, 1994)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhanced by
      NOT Enhanced by
      Suppressed by
      Statement
      Reference

      RafUAS.F179, Scer\GAL4esg.PU has increased occurrence of cell division | adult stage phenotype, suppressible | partially by EMC1UAS.Tag:FLAG, Scer\GAL4esg.PU

      (Shi et al., 2023)

      RafUAS.F179, Scer\GAL4esg.PU has increased occurrence of cell division | adult stage phenotype, suppressible | partially by EMC3UAS.Tag:MYC, Scer\GAL4esg.PU

      (Shi et al., 2023)

      RafUAS.F179, Scer\GAL4esg.PU has abnormal cell number | adult stage phenotype, suppressible | partially by EMC1UAS.Tag:FLAG, Scer\GAL4esg.PU

      (Shi et al., 2023)

      RafUAS.F179, Scer\GAL4esg.PU has abnormal cell number | adult stage phenotype, suppressible | partially by EMC3UAS.Tag:MYC, Scer\GAL4esg.PU

      (Shi et al., 2023)

      RafUAS.F179, Scer\GAL4Delta-05151-G has increased occurrence of cell division | adult stage phenotype, suppressible by dindRNAi.UAS.RNAi, Scer\GAL4Delta-05151-G

      (Ren et al., 2022)

      RafUAS.F179, Scer\GAL4Delta-05151-G has increased cell number | adult stage phenotype, suppressible by dindRNAi.UAS.RNAi, Scer\GAL4Delta-05151-G

      (Ren et al., 2022)

      RafUAS.F179, Scer\GAL4Act.PU has increased cell number | somatic clone | adult stage phenotype, suppressible by dindΔ9/dindΔ9

      (Ren et al., 2022)

      RafUAS.F179, Scer\GAL4Act5C.PI has increased cell number | somatic clone | adult stage phenotype, suppressible by Phb120/Phb120

      (Zhao et al., 2022)

      RafUAS.F179, Scer\GAL4Act5C.PI has increased cell number | somatic clone | adult stage phenotype, suppressible by Phb2Δ/Phb2Δ

      (Zhao et al., 2022)

      RafUAS.F179, Scer\GAL4esg.PU has increased occurrence of cell division | adult stage phenotype, suppressible | partially by dindNIG.7705R, Scer\GAL4esg.PU

      (Zhao et al., 2022)

      RafUAS.F179, Scer\GAL4esg.PU has increased occurrence of cell division | adult stage phenotype, suppressible | partially by dindRNAi.UAS.RNAi, Scer\GAL4esg.PU

      (Zhao et al., 2022)

      RafUAS.F179, Scer\GAL4esg.PU has increased cell number | adult stage phenotype, suppressible by dindRNAi.UAS.RNAi, Scer\GAL4esg.PU

      (Ren et al., 2022, Zhao et al., 2022)

      RafUAS.F179, Scer\GAL4esg.PU has increased occurrence of cell division | adult stage phenotype, suppressible by Phb1HMS00399, Scer\GAL4esg.PU

      (Zhao et al., 2022)

      RafUAS.F179, Scer\GAL4esg.PU has increased occurrence of cell division | adult stage phenotype, suppressible by Phb1HMS00702, Scer\GAL4esg.PU

      (Zhao et al., 2022)

      RafUAS.F179, Scer\GAL4esg.PU has increased occurrence of cell division | adult stage phenotype, suppressible by Phb2HMS02001, Scer\GAL4esg.PU

      (Zhao et al., 2022)

      RafUAS.F179, Scer\GAL4esg.PU has increased occurrence of cell division | adult stage phenotype, suppressible by Phb2GD8538, Scer\GAL4esg.PU

      (Zhao et al., 2022)

      RafUAS.F179, Scer\GAL4Act.PU, scrib1 has increased mortality during development | somatic clone - tissue specific phenotype, suppressible by bskDN.UAS/ZnT86DKK101698, Scer\GAL4Act.PU

      (Wei et al., 2021)

      RafUAS.F179, Scer\GAL4Act.PU, scrib1 has increased mortality during development | somatic clone - tissue specific phenotype, suppressible by bskDN.UAS, Scer\GAL4Act.PU

      (Wei et al., 2021)

      RafUAS.F179, Scer\GAL4Act.PU, scrib1 has neoplasia | larval stage | somatic clone - tissue specific phenotype, suppressible by bskDN.UAS, Scer\GAL4Act.PU

      (Wei et al., 2021)

      RafUAS.F179, Scer\GAL4Act.PU, scrib1 has neoplasia | larval stage | somatic clone - tissue specific phenotype, suppressible by bskDN.UAS/ZnT86DKK101698, Scer\GAL4Act.PU

      (Wei et al., 2021)

      RafUAS.F179, RhoGEF2RE.UAS, Scer\GAL4Tub.PU has neoplasia | somatic clone | third instar larval stage phenotype, suppressible | somatic clone by Rho1GD4726, Scer\GAL4Tub.PU

      (Khoo et al., 2013)

      RafUAS.F179, RhoGEF2RE.UAS, Scer\GAL4Tub.PU has abnormal developmental rate | somatic clone | larval stage phenotype, suppressible | somatic clone by Rho1GD4726, Scer\GAL4Tub.PU

      (Khoo et al., 2013)

      RafUAS.F179, RhoGEF2RE.UAS, Scer\GAL4Tub.PU has neoplasia | somatic clone | third instar larval stage phenotype, suppressible | somatic clone by RokGD1522, Scer\GAL4Tub.PU

      (Khoo et al., 2013)

      RafUAS.F179, RhoGEF2RE.UAS, Scer\GAL4Tub.PU has abnormal developmental rate | somatic clone | larval stage phenotype, suppressible | somatic clone by RokGD1522, Scer\GAL4Tub.PU

      (Khoo et al., 2013)

      RafUAS.F179, RhoGEF2RE.UAS, Scer\GAL4Tub.PU has neoplasia | somatic clone | third instar larval stage phenotype, suppressible | somatic clone by zipGD1566, Scer\GAL4Tub.PU

      (Khoo et al., 2013)

      RafUAS.F179, RhoGEF2RE.UAS, Scer\GAL4Tub.PU has abnormal developmental rate | somatic clone | larval stage phenotype, suppressible | somatic clone by zipGD1566, Scer\GAL4Tub.PU

      (Khoo et al., 2013)

      RafUAS.F179, Scer\GAL4bbg-C96 has visible phenotype, suppressible | partially by gemininUAS.cQa/brmK804R.UAS.Tag:HA, Scer\GAL4bbg-C96

      (Herr et al., 2010)

      RafUAS.F179, Scer\GAL4bbg-C96 has visible phenotype, suppressible | partially by brm[+]/brm2, Scer\GAL4bbg-C96

      (Herr et al., 2010)

      RafUAS.F179, Scer\GAL4hs.2sev has visible phenotype, suppressible by S6kIIUAS.Tag:MYC, Scer\GAL4hs.2sev

      (Kim et al., 2006)

      RafUAS.F179, Scer\GAL4hs.2sev has visible phenotype, suppressible by S6kIIK231R.UAS.Tag:MYC, Scer\GAL4hs.2sev

      (Kim et al., 2006)

      RafUAS.F179, Scer\GAL4Act5C.PI, scrib1 has lethal | somatic clone | larval stage phenotype, suppressible by hepAct.UAS, Scer\GAL4Act5C.PI

      (Uhlirova et al., 2005)

      RafUAS.F179, Scer\GAL471B has lethal phenotype, suppressible by styUAS.cHa, Scer\GAL471B

      (Reich et al., 1999)
      NOT suppressed by
      Enhancer of
      NOT Enhancer of
      Suppressor of
      NOT Suppressor of
      Other
      Statement
      Reference

      RafUAS.F179, Scer\GAL4Act.PU, scrib1 has neoplasia | larval stage | somatic clone - tissue specific phenotype

      (Wei et al., 2021)

      RafUAS.F179, Scer\GAL4Act.PU, scrib1 has majority die during larval stage | somatic clone - tissue specific phenotype

      (Wei et al., 2021)

      RafUAS.F179, Scer\GAL4Act.PU, ZnT86DKK101698, scrib1 has majority die during larval stage | somatic clone - tissue specific phenotype

      (Wei et al., 2021)

      Nhe2null, RafUAS.F179, Scer\GAL4GMR.PU has cell lethal phenotype

      (Grillo-Hill et al., 2015)

      RafUAS.F179, RhoGEF2RE.UAS, Scer\GAL4Tub.PU has abnormal developmental rate | somatic clone | larval stage phenotype

      (Khoo et al., 2013)

      RafUAS.F179, RhoGEF2RE.UAS, Scer\GAL4Tub.PU has neoplasia | somatic clone | third instar larval stage phenotype

      (Khoo et al., 2013)

      Apc2g10, ApcQ8, RafUAS.F179, Scer\GAL4Act.PU has abnormal cell polarity | somatic clone phenotype

      (Wang et al., 2013)

      PlzfUAS.cMa, RafUAS.F179, Scer\GAL4Bx-MS1096 has lethal | pupal stage phenotype

      (Maeng et al., 2012)

      RafUAS.F179, Scer\GAL4Act5C.PI, scrib1 has lethal | somatic clone | larval stage phenotype

      (Uhlirova et al., 2005)

      RafUAS.F179, Scer\GAL4Act5C.PI, scrib1 has neoplasia | somatic clone | larval stage phenotype

      (Uhlirova et al., 2005)

      RafUAS.F179, Scer\GAL4Act5C.PI, hepAct.UAS has increased cell death | somatic clone | larval stage phenotype

      (Uhlirova et al., 2005)

      RafUAS.F179, Scer\GAL4Act5C.PI, hepAct.UAS, scrib1 has neoplasia | somatic clone | cell non-autonomous | adult stage phenotype

      (Uhlirova et al., 2005)

      RafUAS.F179, Scer\GAL4Act5C.PI, hepAct.UAS has neoplasia | somatic clone | cell non-autonomous | adult stage phenotype

      (Uhlirova et al., 2005)

      RafUAS.F179, Scer\GAL4sca-537.4, edΔECD.UAS has visible phenotype

      (Escudero et al., 2003)

      RafUAS.F179, Scer\GAL471B, styUAS.cHa has visible phenotype

      (Reich et al., 1999)
      Phenotype Manifest In
      Enhanced by
      Statement
      Reference

      RafUAS.F179, Scer\GAL4Act.PU, scrib1 has eye-antennal disc | larval stage | somatic clone - tissue specific phenotype, enhanceable by ZnT86DKK101698, Scer\GAL4Act.PU

      (Wei et al., 2021)

      RafUAS.F179, Scer\GAL4Act.PU, scrib1 has larval brain | larval stage | somatic clone - tissue specific phenotype, enhanceable by ZnT86DKK101698, Scer\GAL4Act.PU

      (Wei et al., 2021)

      RafUAS.F179, Scer\GAL4Act.PU, scrib1 has gonad | larval stage | somatic clone - tissue specific phenotype, enhanceable by ZnT86DKK101698, Scer\GAL4Act.PU

      (Wei et al., 2021)

      RafUAS.F179, Scer\GAL4Act.PU, scrib1 has larval ventral nerve cord | larval stage | somatic clone - tissue specific phenotype, enhanceable by ZnT86DKK101698, Scer\GAL4Act.PU

      (Wei et al., 2021)

      RafUAS.F179, Scer\GAL4Act.PU, scrib1 has embryonic/larval fat body | larval stage | somatic clone - tissue specific phenotype, enhanceable by ZnT86DKK101698, Scer\GAL4Act.PU

      (Wei et al., 2021)

      RafUAS.F179, Scer\GAL4Act.PU, scrib1 has embryonic/larval Malpighian tubule | larval stage | somatic clone - tissue specific phenotype, enhanceable by ZnT86DKK101698, Scer\GAL4Act.PU

      (Wei et al., 2021)

      RafUAS.F179, Scer\GAL4Act.PU, scrib1 has eye disc | larval stage phenotype, enhanceable by ZnT86DKK101698, Scer\GAL4Act.PU

      (Wei et al., 2021)

      RafUAS.F179, Scer\GAL4Act.PU, scrib1 has autophagosome | larval stage phenotype, enhanceable by ZnT86DKK101698, Scer\GAL4Act.PU

      (Wei et al., 2021)

      RafUAS.F179, Scer\GAL4dpp.PU has eye-antennal disc | larval stage phenotype, enhanceable by Dis3KK101473, Scer\GAL4dpp.PU

      (Snee et al., 2016)

      RafUAS.F179, Scer\GAL4dpp.PU has wing disc | larval stage phenotype, enhanceable by Dis3KK101473, Scer\GAL4dpp.PU

      (Snee et al., 2016)

      RafUAS.F179, Scer\GAL4Bx-MS1096 has wing disc | larval stage phenotype, enhanceable by Dis3KK101473, Scer\GAL4Bx-MS1096

      (Snee et al., 2016)

      RafUAS.F179, Scer\GAL4GMR.PU has retina phenotype, enhanceable by Nhe2null, Scer\GAL4GMR.PU

      (Grillo-Hill et al., 2015)

      RafUAS.F179, Scer\GAL4GMR.PU has rhabdomere phenotype, enhanceable by Nhe2null, Scer\GAL4GMR.PU

      (Grillo-Hill et al., 2015)

      RafUAS.F179, Scer\GAL4bbg-C96 has wing margin phenotype, enhanceable by geminin[+]/geminink03202b, Scer\GAL4bbg-C96

      (Herr et al., 2010)

      RafUAS.F179, Scer\GAL4bbg-C96 has wing margin phenotype, enhanceable by geminin[+]/geminink14019, Scer\GAL4bbg-C96

      (Herr et al., 2010)

      RafUAS.F179, Scer\GAL4hs.2sev has ommatidium phenotype, enhanceable by S6kIID1/S6kII[+]

      (Kim et al., 2006)

      RafUAS.F179, Scer\GAL4hs.2sev has interommatidial bristle phenotype, enhanceable by S6kIID1/S6kII[+]

      (Kim et al., 2006)

      RafUAS.F179, Scer\GAL4hs.2sev has rhabdomere phenotype, enhanceable by S6kIID1/S6kII[+]

      (Kim et al., 2006)

      RafUAS.F179, Scer\GAL4sev.PU has ommatidium | adult stage phenotype, enhanceable by Mkp3P3

      (Kim et al., 2004)

      RafUAS.F179, Scer\GAL4sev.PU has eye phenotype, enhanceable by Mkp3P3

      (Kim et al., 2004)

      RafUAS.F179, Scer\GAL4sev.PU has eye photoreceptor cell | adult stage | increased number phenotype, enhanceable by Mkp3P3

      (Kim et al., 2004)
      NOT Enhanced by
      Suppressed by
      Statement
      Reference

      RafUAS.F179, Scer\GAL4esg.PU has intestinal stem cell | increased number | adult stage phenotype, suppressible | partially by EMC1UAS.Tag:FLAG, Scer\GAL4esg.PU

      (Shi et al., 2023)

      RafUAS.F179, Scer\GAL4esg.PU has intestinal stem cell | increased number | adult stage phenotype, suppressible | partially by EMC3UAS.Tag:MYC, Scer\GAL4esg.PU

      (Shi et al., 2023)

      RafUAS.F179, Scer\GAL4esg.PU has adult midgut phenotype, suppressible | partially by EMC1UAS.Tag:FLAG, Scer\GAL4esg.PU

      (Shi et al., 2023)

      RafUAS.F179, Scer\GAL4esg.PU has adult midgut phenotype, suppressible | partially by EMC3UAS.Tag:MYC, Scer\GAL4esg.PU

      (Shi et al., 2023)

      RafUAS.F179, Scer\GAL4esg.PU has adult posterior midgut epithelium phenotype, suppressible by dindRNAi.UAS.RNAi, Scer\GAL4esg.PU

      (Ren et al., 2022)

      RafUAS.F179, Scer\GAL4Delta-05151-G has intestinal stem cell of posterior adult midgut epithelium | increased number phenotype, suppressible by dindRNAi.UAS.RNAi, Scer\GAL4Delta-05151-G

      (Ren et al., 2022)

      RafUAS.F179, Scer\GAL4Act.PU has adult posterior midgut epithelium | somatic clone phenotype, suppressible by dindΔ9/dindΔ9

      (Ren et al., 2022)

      RafUAS.F179, Scer\GAL4Act5C.PI has adult posterior midgut epithelium | somatic clone phenotype, suppressible by Phb120/Phb120

      (Zhao et al., 2022)

      RafUAS.F179, Scer\GAL4Act5C.PI has adult posterior midgut epithelium | somatic clone phenotype, suppressible by Phb2Δ/Phb2Δ

      (Zhao et al., 2022)

      RafUAS.F179, Scer\GAL4esg.PU has adult posterior midgut epithelium phenotype, suppressible | partially by dindRNAi.UAS.RNAi, Scer\GAL4esg.PU

      (Zhao et al., 2022)

      RafUAS.F179, Scer\GAL4esg.PU has intestinal stem cell of posterior adult midgut epithelium | increased number phenotype, suppressible | partially by dindRNAi.UAS.RNAi, Scer\GAL4esg.PU

      (Zhao et al., 2022)

      RafUAS.F179, Scer\GAL4esg.PU has adult posterior midgut epithelium phenotype, suppressible by Phb1HMS00399, Scer\GAL4esg.PU

      (Zhao et al., 2022)

      RafUAS.F179, Scer\GAL4esg.PU has adult posterior midgut epithelium phenotype, suppressible by Phb1HMS00702, Scer\GAL4esg.PU

      (Zhao et al., 2022)

      RafUAS.F179, Scer\GAL4esg.PU has adult posterior midgut epithelium phenotype, suppressible by Phb2GD8538, Scer\GAL4esg.PU

      (Zhao et al., 2022)

      RafUAS.F179, Scer\GAL4esg.PU has adult posterior midgut epithelium phenotype, suppressible by Phb2HMS02001, Scer\GAL4esg.PU

      (Zhao et al., 2022)

      RafUAS.F179, Scer\GAL4Act.PU, scrib1 has eye-antennal disc | larval stage | somatic clone - tissue specific phenotype, suppressible by bskDN.UAS/ZnT86DKK101698, Scer\GAL4Act.PU

      (Wei et al., 2021)

      RafUAS.F179, Scer\GAL4Act.PU, scrib1 has eye-antennal disc | larval stage | somatic clone - tissue specific phenotype, suppressible by bskDN.UAS, Scer\GAL4Act.PU

      (Wei et al., 2021)

      RafUAS.F179, Scer\GAL4Act.PU, scrib1 has gonad | larval stage | somatic clone - tissue specific phenotype, suppressible by bskDN.UAS/ZnT86DKK101698, Scer\GAL4Act.PU

      (Wei et al., 2021)

      RafUAS.F179, Scer\GAL4Act.PU, scrib1 has gonad | larval stage | somatic clone - tissue specific phenotype, suppressible by bskDN.UAS, Scer\GAL4Act.PU

      (Wei et al., 2021)

      RafUAS.F179, Scer\GAL4Act.PU, scrib1 has larval brain | larval stage | somatic clone - tissue specific phenotype, suppressible by bskDN.UAS/ZnT86DKK101698, Scer\GAL4Act.PU

      (Wei et al., 2021)

      RafUAS.F179, Scer\GAL4Act.PU, scrib1 has larval brain | larval stage | somatic clone - tissue specific phenotype, suppressible by bskDN.UAS, Scer\GAL4Act.PU

      (Wei et al., 2021)

      RafUAS.F179, Scer\GAL4Act.PU, scrib1 has larval ventral nerve cord | larval stage | somatic clone - tissue specific phenotype, suppressible by bskDN.UAS/ZnT86DKK101698, Scer\GAL4Act.PU

      (Wei et al., 2021)

      RafUAS.F179, Scer\GAL4Act.PU, scrib1 has larval ventral nerve cord | larval stage | somatic clone - tissue specific phenotype, suppressible by bskDN.UAS, Scer\GAL4Act.PU

      (Wei et al., 2021)

      RafUAS.F179, Scer\GAL4Act.PU, scrib1 has eye disc | larval stage phenotype, suppressible | partially by bskDN.UAS, Scer\GAL4Act.PU

      (Wei et al., 2021)

      RafUAS.F179, Scer\GAL4Act.PU, scrib1 has autophagosome | larval stage phenotype, suppressible | partially by bskDN.UAS, Scer\GAL4Act.PU

      (Wei et al., 2021)

      RafUAS.F179, Scer\GAL4Act.PU, scrib1 has eye disc | larval stage phenotype, suppressible | partially by bskDN.UAS/ZnT86DKK101698, Scer\GAL4Act.PU

      (Wei et al., 2021)

      RafUAS.F179, Scer\GAL4Act.PU, scrib1 has autophagosome | larval stage phenotype, suppressible | partially by bskDN.UAS/ZnT86DKK101698, Scer\GAL4Act.PU

      (Wei et al., 2021)

      RafUAS.F179, RhoGEF2RE.UAS, Scer\GAL4Tub.PU has eye-antennal disc | somatic clone | third instar larval stage phenotype, suppressible | partially | somatic clone by Rho1GD4726, Scer\GAL4Tub.PU

      (Khoo et al., 2013)

      RafUAS.F179, RhoGEF2RE.UAS, Scer\GAL4Tub.PU has eye photoreceptor cell | somatic clone | third instar larval stage phenotype, suppressible | partially | somatic clone by Rho1GD4726, Scer\GAL4Tub.PU

      (Khoo et al., 2013)

      RafUAS.F179, RhoGEF2RE.UAS, Scer\GAL4Tub.PU has eye-antennal disc | somatic clone | third instar larval stage phenotype, suppressible | partially | somatic clone by RokGD1522, Scer\GAL4Tub.PU

      (Khoo et al., 2013)

      RafUAS.F179, RhoGEF2RE.UAS, Scer\GAL4Tub.PU has eye photoreceptor cell | somatic clone | third instar larval stage phenotype, suppressible | partially | somatic clone by RokGD1522, Scer\GAL4Tub.PU

      (Khoo et al., 2013)

      RafUAS.F179, RhoGEF2RE.UAS, Scer\GAL4Tub.PU has eye-antennal disc | somatic clone | third instar larval stage phenotype, suppressible | partially | somatic clone by zipGD1566, Scer\GAL4Tub.PU

      (Khoo et al., 2013)

      RafUAS.F179, RhoGEF2RE.UAS, Scer\GAL4Tub.PU has eye photoreceptor cell | somatic clone | third instar larval stage phenotype, suppressible | partially | somatic clone by zipGD1566, Scer\GAL4Tub.PU

      (Khoo et al., 2013)

      RafUAS.F179, Scer\GAL4bbg-C96 has wing margin phenotype, suppressible | partially by gemininUAS.cQa/brmK804R.UAS.Tag:HA, Scer\GAL4bbg-C96

      (Herr et al., 2010)

      RafUAS.F179, Scer\GAL4bbg-C96 has wing margin phenotype, suppressible | partially by brm[+]/brm2, Scer\GAL4bbg-C96

      (Herr et al., 2010)

      RafUAS.F179, putΔI.UAS has wing vein phenotype, suppressible by Scer\GAL4e22c, putΔI.UAS

      (Yan et al., 2009)

      RafUAS.F179, Scer\GAL4e22c has wing cell phenotype, suppressible by putΔI.UAS, Scer\GAL4e22c

      (Yan et al., 2009)

      RafUAS.F179, Scer\GAL4hs.PB has wing vein phenotype, suppressible by putΔI.UAS, Scer\GAL4hs.PB

      (Yan et al., 2009)

      RafUAS.F179, Scer\GAL4hs.PB has wing cell phenotype, suppressible by putΔI.UAS, Scer\GAL4hs.PB

      (Yan et al., 2009)

      RafUAS.F179, Scer\GAL4hs.2sev has ommatidium phenotype, suppressible by S6kIIUAS.Tag:MYC, Scer\GAL4hs.2sev

      (Kim et al., 2006)

      RafUAS.F179, Scer\GAL4hs.2sev has interommatidial bristle phenotype, suppressible by S6kIIUAS.Tag:MYC, Scer\GAL4hs.2sev

      (Kim et al., 2006)

      RafUAS.F179, Scer\GAL4hs.2sev has rhabdomere phenotype, suppressible by S6kIIK231R.UAS.Tag:MYC, Scer\GAL4hs.2sev

      (Kim et al., 2006)

      RafUAS.F179, Scer\GAL4hs.2sev has ommatidium phenotype, suppressible by S6kIIK231R.UAS.Tag:MYC, Scer\GAL4hs.2sev

      (Kim et al., 2006)

      RafUAS.F179, Scer\GAL4hs.2sev has interommatidial bristle phenotype, suppressible by S6kIIK231R.UAS.Tag:MYC, Scer\GAL4hs.2sev

      (Kim et al., 2006)

      RafUAS.F179, Scer\GAL4hs.2sev has rhabdomere phenotype, suppressible by S6kIIUAS.Tag:MYC, Scer\GAL4hs.2sev

      (Kim et al., 2006)
      NOT suppressed by
      Statement
      Reference

      RafUAS.F179, Scer\GAL4sev.PU has eye phenotype, non-suppressible by FakUAS.Tag:HA, Scer\GAL4sev.PU

      (Macagno et al., 2014)

      RafUAS.F179, Scer\GAL4Tub.PU has eye-antennal disc | somatic clone | third instar larval stage phenotype, non-suppressible | somatic clone by Rho1GD4726, Scer\GAL4Tub.PU

      (Khoo et al., 2013)

      RafUAS.F179, Scer\GAL4Tub.PU has eye photoreceptor cell | somatic clone | third instar larval stage phenotype, non-suppressible | somatic clone by Rho1GD4726, Scer\GAL4Tub.PU

      (Khoo et al., 2013)

      RafUAS.F179, Scer\GAL4Tub.PU has eye-antennal disc | somatic clone | third instar larval stage phenotype, non-suppressible | somatic clone by RokGD1522, Scer\GAL4Tub.PU

      (Khoo et al., 2013)

      RafUAS.F179, Scer\GAL4Tub.PU has eye photoreceptor cell | somatic clone | third instar larval stage phenotype, non-suppressible | somatic clone by RokGD1522, Scer\GAL4Tub.PU

      (Khoo et al., 2013)

      RafUAS.F179, Scer\GAL4Tub.PU has eye-antennal disc | somatic clone | third instar larval stage phenotype, non-suppressible | somatic clone by zipGD1566, Scer\GAL4Tub.PU

      (Khoo et al., 2013)

      RafUAS.F179, Scer\GAL4Tub.PU has eye photoreceptor cell | somatic clone | third instar larval stage phenotype, non-suppressible | somatic clone by zipGD1566, Scer\GAL4Tub.PU

      (Khoo et al., 2013)

      RafUAS.F179, RhoGEF2RE.UAS, Scer\GAL4Tub.PU has eye-antennal disc | somatic clone | third instar larval stage phenotype, non-suppressible | somatic clone by LIMK1GD9586, Scer\GAL4Tub.PU

      (Khoo et al., 2013)

      RafUAS.F179, RhoGEF2RE.UAS, Scer\GAL4Tub.PU has eye photoreceptor cell | somatic clone | third instar larval stage phenotype, non-suppressible | somatic clone by LIMK1GD9586, Scer\GAL4Tub.PU

      (Khoo et al., 2013)

      RafUAS.F179, Scer\GAL4bbg-C96 has wing margin phenotype, non-suppressible by brmK804R.UAS.Tag:HA, Scer\GAL4bbg-C96

      (Herr et al., 2010)

      RafUAS.F179, Scer\GAL4bbg-C96 has wing margin phenotype, non-suppressible by gemininUAS.cQa, Scer\GAL4bbg-C96

      (Herr et al., 2010)

      RafUAS.F179, Scer\GAL4GMR.PU has eye phenotype, non-suppressible by Rabex-5UAS.Tag:MYC, Scer\GAL4GMR.PU

      (Yan et al., 2010)

      RafUAS.F179, Scer\GAL4da.G32 has eye photoreceptor cell | ectopic phenotype, non-suppressible by ave108V

      (Roignant et al., 2006)
      Enhancer of
      Suppressor of
      Other
      Statement
      Reference

      RafUAS.F179, Scer\GAL4Act.PU, scrib1 has eye disc | larval stage phenotype

      (Wei et al., 2021)

      RafUAS.F179, Scer\GAL4Act.PU, scrib1 has autophagosome | larval stage phenotype

      (Wei et al., 2021)

      RafUAS.F179, Scer\GAL4Act.PU, scrib1 has larval ventral nerve cord | larval stage | somatic clone - tissue specific phenotype

      (Wei et al., 2021)

      RafUAS.F179, Scer\GAL4Act.PU, scrib1 has larval brain | larval stage | somatic clone - tissue specific phenotype

      (Wei et al., 2021)

      RafUAS.F179, Scer\GAL4Act.PU, scrib1 has eye-antennal disc | larval stage | somatic clone - tissue specific phenotype

      (Wei et al., 2021)

      RafUAS.F179, Scer\GAL4Act.PU, scrib1 has gonad | larval stage | somatic clone - tissue specific phenotype

      (Wei et al., 2021)

      RafUAS.F179, Scer\GAL4Act.PU, scrib1 has embryonic/larval fat body | larval stage | somatic clone - tissue specific phenotype

      (Wei et al., 2021)

      RafUAS.F179, Scer\GAL4Act.PU, scrib1 has embryonic/larval Malpighian tubule | larval stage | somatic clone - tissue specific phenotype

      (Wei et al., 2021)

      Apc2g10, ApcQ8, RafUAS.F179, Scer\GAL4Act.PU has intestinal stem cell | somatic clone phenotype

      (Wang et al., 2013)

      Apc2g10, ApcQ8, RafUAS.F179, Scer\GAL4Act.PU has adult midgut | somatic clone phenotype

      (Wang et al., 2013)

      RafUAS.F179, Scer\GAL4Act5C.PI, hepAct.UAS has retina | somatic clone phenotype

      (Uhlirova et al., 2005)

      RafUAS.F179, Scer\GAL4Act5C.PI, hepAct.UAS, scrib1 has adult head | somatic clone phenotype

      (Uhlirova et al., 2005)

      RafUAS.F179, Scer\GAL4Act5C.PI, hepAct.UAS, scrib1 has retina | somatic clone phenotype

      (Uhlirova et al., 2005)

      RafUAS.F179, Scer\GAL4Act5C.PI, hepAct.UAS has adult head | somatic clone phenotype

      (Uhlirova et al., 2005)

      RafUAS.F179, Scer\GAL4sca-537.4, edΔECD.UAS has macrochaeta phenotype

      (Escudero et al., 2003)

      RafUAS.F179, Scer\GAL4GMR.PY, pntP2.UAS has ommatidial precursor cluster phenotype

      (Yamada et al., 2003)

      RafUAS.F179, Scer\GAL4GMR.PY, pntP2.UAS has photoreceptor cell R4 phenotype

      (Yamada et al., 2003)

      RafUAS.F179, Scer\GAL4GMR.PY, pntP2.UAS has photoreceptor cell R3 phenotype

      (Yamada et al., 2003)

      RafUAS.F179, Scer\GAL4GMR.PY, pntP2.UAS has photoreceptor cell R1 phenotype

      (Yamada et al., 2003)

      RafUAS.F179, Scer\GAL4GMR.PY, pntP2.UAS has photoreceptor cell R6 phenotype

      (Yamada et al., 2003)

      RafUAS.F179, Scer\GAL4en-e16E, pntP2.UAS has abdominal lateral pentascolopidial chordotonal organ lch5 phenotype

      (Yamada et al., 2003)

      RafUAS.F179, Scer\GAL471B, styUAS.cHa has wing vein phenotype

      (Reich et al., 1999)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Eye-disc specific clones (using ey-FLP) homozygous for scrib1 and expressing RafUAS.F179 under the control of Scer\GAL4Act.PU develop invasive tumors in larval gonads, eye-antennal discs and brain; there is metastization to the ventral nerve cord, fat body and Malpighian tubules.

      (Wei et al., 2021)

      Co-expression of Dis3KK101473 significantly enhances tissue overgrowth in eye-antennal imaginal or wing discs with expression of RafScer\UAS.F179 driven by Scer\GAL4dpp.PU (or in wing discs driven by Scer\GAL4Bx-MS1096).

      (Snee et al., 2016)

      Expression of phlScer\UAS.F179 under the control of Scer\GAL4GMR.PU in a Nhe2null mutant background results in synthetic cell lethality. Externally, the adult eye phenotype is identical to expression of phlScer\UAS.F179 alone, except that eye colour is lighter, but internally there is loss of tissue architecture and cell structure with coagulative necrosis, including loss of rhabdomere morphology, absence of distinct cell membranes and diffusion of pigment granules.

      Nhe2null suppresses the tissue morphology defects and hyperproliferation seen in third instar larval eye disc when phlScer\UAS.F179 is expressed under the control of Scer\GAL4GMR.PU. Neuronal cell fates appear unaffected.

      Nhe2null enhances the morphological defects seen in the retina of pupal expressing phlScer\UAS.F179 under the control of Scer\GAL4GMR.PU. All cell types appear smaller and more cells have abnormal morphologies. More ommatidia have extra cone cells. Cell death is seen that is not detected in either mutant alone.

      In contrast to either mutant alone, the intracellular pH (pHi) in eye discs expressing phlScer\UAS.F179 under the control of Scer\GAL4GMR.PU in a Nhe2null mutant background is similar to wild type. However, the pHi is reduced compared to wild type by the pupal stages.

      (Grillo-Hill et al., 2015)

      Expression of FakScer\UAS.T:Ivir\HA1 does not suppress the eye phenotypes seen when phlScer\UAS.F179 is expressed under the control of Scer\GAL4sev.PU.

      (Macagno et al., 2014)

      Co-expression of Rho1GD4726, zipGD1566 or RokGD1522 fails to suppress the ectopic differentiation of seen in eye-antennal disc clones expressing RafScer\UAS.F179 under the control of Scer\GAL4tub.PU.

      Co-expression of RafScer\UAS.F179 enhances the decreased photoreceptor differentiation seen in eye-antennal disc clones expressing RhoGEF2RE.Scer\UAS under the control of Scer\GAL4tub.PU. These clones show overgrowth, similar to those expressing only RhoGEF2RE.Scer\UAS, but also show large undifferentiated clonal masses in the basal regions of the posterior region of the eye disc. Larvae containing clones expressing both RhoGEF2RE.Scer\UAS and RafScer\UAS.F179 under the control of Scer\GAL4tub.PU exhibit an extended larval period, as compared with controls.

      Co-expression of LIMK1GD9586 fails to suppress the reduction of differentiation seen in eye-antennal disc clones expressing both RhoGEF2RE.Scer\UAS and RafScer\UAS.F179 under the control of Scer\GAL4tub.PU, and also fails to suppress the developmental delay shown by larvae containing these clones.

      Co-expression of Rho1GD4726, zipGD1566 or RokGD1522 suppresses the formation of undifferentiated clonal masses and partially suppresses the reduction of differentiation seen in eye-antennal disc clones expressing both RhoGEF2RE.Scer\UAS and RafScer\UAS.F179 under the control of Scer\GAL4tub.PU, and also suppresses the developmental delay shown by larvae containing these clones.

      (Khoo et al., 2013)

      Expression of phlScer\UAS.F179 induces cell polarity changes in Apc2g10 ApcQ8 mutant intestinal stem cell clones.

      (Wang et al., 2013)

      Flies that co-express phlScer\UAS.F179 and PlzfScer\UAS.cMa under the control of Scer\GAL4Bx-MS1096 die at the pupal stage due to wing malformation.

      (Maeng et al., 2012)

      Co-expression of gemininScer\UAS.cQa with phlScer\UAS.F179 via Scer\GAL4bbg-C96 does not significantly affect the notched wing phenotype resulting from the expression of phlScer\UAS.F179 alone.

      Co-expression of brmK804R.Scer\UAS.T:Ivir\HA1 with phlScer\UAS.F179 via Scer\GAL4bbg-C96 does not significantly affect the notched wing phenotype resulting from the expression of phlScer\UAS.F179 alone.

      Co-expression of both brmK804R.Scer\UAS.T:Ivir\HA1 and gemininScer\UAS.cQa with phlScer\UAS.F179 via Scer\GAL4bbg-C96 results in a significant suppression of the notched wing phenotype resulting from the expression of phlScer\UAS.F179 alone.

      brm2/+ significantly suppresses the notched wing phenotype resulting from the expression of phlScer\UAS.F179 via Scer\GAL4bbg-C96.

      geminink03202b/+ causes a significant worsening of the notched wing phenotype resulting from the expression of phlScer\UAS.F179 via Scer\GAL4bbg-C96.

      geminink14019/+ causes a significant worsening of the notched wing phenotype resulting from the expression of phlScer\UAS.F179 via Scer\GAL4bbg-C96.

      (Herr et al., 2010)

      The supernumerary spectrosome-containing cell phenotype of stet1A3 mutants is partially suppressed by phlScer\UAS.F179 expression driven by Scer\GAL4C587.

      (Liu et al., 2010)

      Co-expression of Rabex-5VDRC.cUa fails to enhance the eye phenotypes resulting from the overexpression of phlScer\UAS.F179 driven by Scer\GAL4GMR.PU.

      Co-expression of Rabex-5Scer\UAS.T:Hsap\MYC driven by Scer\GAL4GMR.PU does not suppress the appearance of black tissue in phlScer\UAS.F179-expressing eyes.

      (Yan et al., 2010)

      Expression of phlScer\UAS.F179 alone, or combined with the Scer\GAL4c747 driver but kept silent by Scer\GAL80ts.αTub84B does not suppress the learning deficit of drkΔP24/+ animals. Conditional expression of phlScer\UAS.F179 in adult mushroom bodies reverse the learning deficit of drkΔP24 heterozygotes.

      Expression of phlScer\UAS.F179 under the control of Scer\GAL4c747 does not rescue 90 minute memory in drkΔP24/+ animals.

      Expression of phlScer\UAS.F179 under the control of Scer\GAL4c747 results in full reversal of the learning deficit of drke0A heterozygotes.

      (Moressis et al., 2009)

      Coexpression of phlScer\UAS.F179 with putDeltaI.Scer\UAS after a 20 minute heat-shock at 37[o]C at 18h APF, under the control of Scer\GAL4hs.PB, not only fails to induce ectopic vein cells, but also inhibits endogenous vein formation.

      (Yan et al., 2009)

      The roughened eye and increased rhabdomere phenotype seen when phlScer\UAS.F179 is expressed under the control of Scer\GAL4hs.2sev is enhanced in a S6kIID1/+ or S6kIID1/Y background. These eye phenotypes are suppressed when either S6kIIScer\UAS.T:Hsap\MYC or S6kIIK231R.Scer\UAS.T:Hsap\MYC is coexpressed with phlScer\UAS.F179 (all under the control of Scer\GAL4hs.2sev).

      (Kim et al., 2006)

      When phlScer\UAS.F179 is expressed under the control of Scer\GAL4da.G32 in ave108V mutant clones, ectopic photoreceptor cells are induced and the ave108V phenotype is suppressed.

      (Roignant et al., 2006)

      When phlScer\UAS.F179 is driven by Scer\GAL4dpp.blk1 in a ftG-rv background a significant enhancement is seen in the ftG-rv overgrowth phenotype, throughout the whole imaginal disc. When phlScer\UAS.F179 is driven by Scer\GAL4ey.PH in ftG-rv mutant eyes, significantly enlarged eyes are seen.

      (Garoia et al., 2005)

      Expression of hepAct.Scer\UAS in eye imaginal disc clones that express RafScer\UAS.F179 (both under the control of Scer\GAL4Act5C.PI) suppresses the aggressive tissue expansion, resulting in clones of this genotype remaining much smaller than RafScer\UAS.F179 (under the control of Scer\GAL4Act5C.PI) clones. hepAct.Scer\UAS expression can elevate the occurence of apoptosis among RafScer\UAS.F179 expressing cells (from 6.0% to 17.3%). However, a significant fraction of hepAct.Scer\UAS RafScer\UAS.F179 (both under the control of Scer\GAL4Act5C.PI) clones survive (6.4%).

      Clones of eye imaginal discs that express RafScer\UAS.F179 autonomously (under the control of Scer\GAL4Act5C.PI) in scrib1 clones grow into massive and invasive tumours during larval stages, resulting in 80% of animals not pupating and dying as giant larvae.

      Expression of hepAct.Scer\UAS in eye imaginal disc clones that express RafScer\UAS.F179 (under the control of Scer\GAL4Act5C.PI) results in adult eyes with massive overgrowth. The heads are significantly bigger and the retina of these mutants are dramatically larger than that of a wild-type eye. In many cases, the retina is folded and bunched to accommodate the surfeit of tissue. These severely hyperplastic eye structures are well patterned and show a distinctive ommatidial organisation. The tumourous overgrowth is induced non-autonomously in the wild-type cells surrounding the clones. The same phenotype tumorogenic phnotype occurs in flies that express both RafScer\UAS.F179 and hepAct.Scer\UAS, under the control of Scer\GAL4Act5C.PI, in a scrib1 background.

      (Uhlirova et al., 2005)

      When phlScer\UAS.F179 and pntP2.Scer\UAS are coexpressed in the embryo under the control of Scer\GAL4en-e16E, the number of scolopidia in the lch5 organs is reduced from the normal number of 5 to 3 or 4 in 71% of hemisegments. When phlScer\UAS.F179 and pntP2.Scer\UAS are coexpressed under the control of Scer\GAL4GMR.PY in cells posterior to the morphogenetic furrow, most ommatidial clusters in the early stages of ommatidial assembly contain fewer than the normal number of neurons, with the specification of R3, R4, R1 and R6 being severely disrupted.

      (Yamada et al., 2003)

      Lethality of phlScer\UAS.F179, Scer\GAL471B is rescued by styScer\UAS.cHa. Wings of phlScer\UAS.F179, styScer\UAS.cHa, Scer\GAL471B mutants are largely devoid of wing vein.

      (Reich et al., 1999)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Fails to rescue

      RafUAS.F179 fails to rescue RafCR00483-TG4.1

      (Lee et al., 2018)
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (10)
      Reported As
      Symbol Synonym
      D-rafF179
      (Morimoto et al., 1996)
      RafF179
      (Koh et al., 2002)
      RafGOF
      (Wei et al., 2021)
      RafScer\UAS.F179
      RafUAS.F179
      Rafgof
      (Azuma et al., 2021)
      dRafgof
      (Pfeifle et al., 2019)
      phlScer\UAS.F179
      phlUAS.F179
      rafact
      (Uhlirova and Bohmann, 2006)
      Name Synonyms
      Secondary FlyBase IDs
        References (82)