Allele Dmel\tsh^hs.PdZ

General Information
Symbol	Dmel\tsh^hs.PdZ	Species	D. melanogaster
Name	heat shock construct of de Zulueta	FlyBase ID	FBal0040054
Feature type	allele	Associated gene	Dmel\tsh

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (de Zulueta et al., 1994)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Entire coding region of a tsh cDNA expressed from an Hsp70 promoter. (de Zulueta et al., 1994)
Carried in construct	P{HS-tsh} (de Zulueta et al., 1994, Alexandre et al., 1996)
Cytology
Phenotypic Data
Phenotypic Class
	lethal \| heat sensitive (de Zulueta et al., 1994)
Phenotype Manifest In
	denticle belt \| heat sensitive (de Zulueta et al., 1994) labial segment \| heat sensitive (de Zulueta et al., 1994) maxillary segment \| heat sensitive (de Zulueta et al., 1994) procephalic segment \| heat sensitive (de Zulueta et al., 1994)
Detailed Description
	Statement Reference Embryos heat shocked for 20 minutes at 37^oC show trunk-type denticle belts in the head region, though the trunk and tail region are not affected. Embryos treated at the blastoderm stage show defects in head, truck and tail parts. Embryos treated later (stage 6 or later) do not show segmentation defects in a significant proportion of larvae. 38% of larvae treated at or before stage 10 show homeotic transformations of head to trunk identity, most strikingly in the labial segment, where naked cuticle, a prothoracic denticle belt and a T1 beard appear. Not all head structures are suppressed, as maxillary sense organs, cirri and mouth hooks remain. Longer heat shocks cause more extreme transformations of procephalon and maxillary segment occur, though transformation is still not as complete as for the labial segment. In combination with Scr^hs.PG, tsh^hs.PdZ causes more extreme prothoracic transformations in procephalon and maxillary segment than tsh^hs.PdZ alone. tsh^hs.PdZ increases the expressivity of the homeotic transformations caused by Antp^hs.PHTA or Ubx^hs.PG. (de Zulueta et al., 1994)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Partially rescues	tsh^hs.PdZ partially rescues tsh⁸ (de Zulueta et al., 1994) tsh^hs.PdZ partially rescues tsh⁹ (de Zulueta et al., 1994)
Comments	Partially rescues null tsh⁸ or strong tsh⁹ phenotype. (de Zulueta et al., 1994)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	tsh^hs.PdZ
Name Synonym	heat shock construct of de Zulueta (de Zulueta et al., 1994)
Secondary FlyBase IDs

References ( 2 )
Research paper	Alexandre et al., 1996, Mech. Dev. 59(2): 191--204 The Drosophila teashirt homeotic protein is a DNA-binding protein and modulo, a HOM-C regulated modifier of variegation, is a likely candidate for being a direct target gene. [FBrf0090432] de Zulueta et al., 1994, Development 120(8): 2287--2296 Homeotic complex and teashirt genes co-operate to establish trunk segmental identifies in Drosophila. [FBrf0075096]

Allele Dmel\tshhs.PdZ

Allele Dmel\tsh^hs.PdZ