Open Close
General Information
Symbol
Dmel\vir2f
Species
D. melanogaster
Name
FlyBase ID
FBal0040111
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
point mutation
Nucleotide change:

T23362230A

Amino acid change:

M1283K | vir-PA

Reported amino acid change:

M1283K

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Amino acid replacement: M1283K.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

vir2f homozygous females are completely lethal.

vir2f homozygotes are female lethal.

Female-specific allele.

X chromosomal recombination frequencies in female germ cells homozygous for vir2f are only slightly reduced compared to wild-type.

Female germ cells homozygous for vir2f can complete oogenesis. Females carrying these homozygous germ line clones give rise to male and female offspring in a 1:1 ratio when mated to vir+ males and give rise to purely male offspring when mated to homozygous vir2f or virts males. These phenotypes are rescued if the homozygous germ line clones are induced in females carrying vir+t10. Females carrying vir6 homozygous germ line clones give rise only to male offspring when mated to vir2f males, and only a few percent of these males survive to adulthood.

Homozygotes and transheterozygotes with other EMS induced vir mutations exhibit normal male viability and fertility. Transheterozygotes with virts transform XX animals into intersexes at 29oC. Homozygous clones that occur in the sexually dimorphic regions of females differentiate male structures. Clones in males produce normal male structures. Foreleg and genital vir2f/vir6 discs transplanted into vir+ hosts differentiate poorly developed male structures. Sxl+/Sxl+;vir2f/virts and Sxlf1/Sxl+;vir2f/vir+ are viable but doubly heterozygous Sxlf1/Sxl+;vir2f/virts females are lethal.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement
Reference

vir2f has lethal | female phenotype, suppressible | partially by FlaccC/nito[+]/nito1/Flacc[+]

vir2f has lethal | recessive | female phenotype, suppressible | partially by Mettl3Delta22-3/Ime4[+]

vir2f has lethal | female | larval stage phenotype, suppressible by msl-1γ222

vir2f has lethal | female | larval stage phenotype, suppressible by msl-2γ136

vir2f has lethal | female | larval stage phenotype, suppressible by mle9

NOT suppressed by
Statement
Reference

vir2f has lethal | female phenotype, non-suppressible by nito[+]/nito1

vir2f has lethal | female phenotype, non-suppressible by FlaccC/Flacc[+]

Enhancer of
Statement
Reference

vir2f is an enhancer of visible phenotype of SxlMf1, snf+t5.5/snf+t5.5

Suppressor of
Statement
Reference

vir2f/vir22 is a suppressor of lethal | male phenotype of SxlM1

vir23/vir2f is a suppressor of lethal | male phenotype of SxlM1

vir2f/Df(2R)vir15 is a suppressor of lethal | male phenotype of SxlM1

vir2f is a suppressor of lethal phenotype of SxlM12, snf+t5.5/snf+t5.5

Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

The complete lethality of vir2f homozygous females cannot be suppressed by combination with a single copy of nito1 or FlaccC individually but heterozygosity for both does partially restore viability of the vir2f/vir2f females.

The female lethality characteristic for vir2f homozygotes is rescued to a high degree by combination with a single copy of Ime4Δ22-3.

The addition of homozygous vir2f suppresses the lethality seen in P{snf+,dhd+}, SxlM12/Y males.

SxlM4/+ ; vir6/vir2f animals derived from females carrying homozygous vir6 germ line clones mated to vir2f/+ males survive, but are sexually transformed. SxlM4/+ ; vir2f/vir2f animals derived from females carrying homozygous vir2f germ line clones mated to homozygous vir2f males survive, but are sexually transformed. SxlM1 cannot rescue the XX progeny of females carrying homozygous vir2f germ line clones mated to homozygous vir2f males whether it is paternally or maternally inherited.

Female lethality can be rescued by msl-1γ222, msl-2γ136 or mle9 mutations, rescued animals are transformed into pseudomales. The presence of SxlM1, SxlM4 or SxlF1.hs rescues the female lethality of vir transheterozygotes, animals are strongly macsculinised and in some cases transformed to pseudomales.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Rescued by
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
Comments
Comments

Homozygous larvae and heteroallelic larvae with vir3, reared at 25oC, have msl-1 and mle associated with numerous sites along the paired X chromosomes.

Protein is defective in functions required for dosage compensation and sex determination.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (12)