wgl-12.Scer\UAS; Scer\GAL4dpp.blk1 suppresses duplication of the wing pouch and prospective notum in ds38k homozygous wing discs, although the hinge domain is expanded compared with the wild type. Normal anterior-posterior subdivision is also restored in these discs. These animals die before reaching adulthood.
Co-expression of wgl-12.Scer\UAS can rescue both the wing notching phenotype and loss of sensory bristles caused by expression of dlpScer\UAS.cBa under the control of Scer\GAL4C96. In addition, ectopic margin bristles close to the wing margin are seen in these flies.
The addition of wgl-12.Scer\UAS suppresses the wing pouch phenotype seen in NECN.Scer\UAS/Scer\GAL4sd-SG29.1 wing discs, and abolishes the cell death phenotype. Wings in these adult flies are almost wild-type in size and in many instances display a partial restoration of bristles at the edge. In addition these wings display enlarged veins which are much broader than those produced by either NECN.Scer\UAS or wgl-12.Scer\UAS alone. The addition of wgl-12.Scer\UAS strongly suppresses the wing phenotype seen in NECNΔ10-12.Scer\UAS/Scer\GAL4sd-SG29.1 flies, restoring the wing almost to wild-type, and partially suppresses the wing phenotype seen in NECNΔ17-19;24-26.Scer\UAS/Scer\GAL4sd-SG29.1 flies. The addition of wgl-12.Scer\UAS (driven by Scer\GAL4sd-SG29.1) to Df(3R)Dl-FX3/+ flies leads to a dramatic increase in the width of the wing veins.
The lack of denticles seen in embryos expressing wgl-12.Scer\UAS under the control of Scer\GAL4arm.PS is suppressed by injection of both dsRNA produced by annealing fz374-2360 and fza.374-2360 RNA and dsRNA produced by annealing fz2L.cKa and fz2a.L.cKa RNA; a pattern of denticles similar to wild-type is restored and ectopic denticles are produced in regions where naked cuticle should be secreted.
Coexpression of wgl-12.Scer\UAS and SerScer\UAS.cSa with Scer\GAL4sd-SG29.1 the resulting flies bear large wings that are covered in bristles. The wings lack a defined margin and are more round that elongated along the PD axis. Coexpression with Scer\GAL4ptc-559.1 leads to the production of extra wings off the proximal dorsal side of the wing.
Scer\GAL4ptc-559.1-mediated coexpression with SerScer\UAS.cSa causes outgrowth on the ventral side of the wing discs and a small amount on the dorsal side but the nick in the wing margin is repressed. Ectopic wing tissue is also found in the compound eye and legs. Scer\GAL4h-540.3-mediated expression in SerRX82/Df(3R)D605 hemizygous second/early third larval instar fails to rescue the rudimentary wing phenotype.
Scer\GAL4arm.PS mediated expression of wgl-12.Scer\UAS in wgl-17 Df(2R)enE embryos at varying temperatures can be used to study the dose response to wg for A cells. At 17.5oC wgl-12.Scer\UAS produces the same phenotype as when wild type wg (wgScer\UAS.cLa) is added. At 20oC there are only a few ventral denticles and some beard in T1. At 22oC there are two lateral stripes of ventral denticles and some beard in T1. At 23oC the abdomen is completely covered in denticles but there is no beard in T1. At 25oC the abdomen is completely covered in denticles, weak thoracic denticles are present, but there is no beard in T1. At 28.5oC embryos are indistinguishable from wgl-17 Df(2R)enE embryo.
Scer\GAL4ptc-559.1 induced co-expression of wgl-12.Scer\UAS and SerScer\UAS.cSa causes a duplicated pair of extra wings that arise from the proximal dorsal side of the endogenous wings. The endogenous wings may exhibit extra margin material. Wing tissues appear, with characteristic marginal bristles, from the proximal dorsal side in all legs. Scer\GAL4h-1J3 induced coexpression of wgl-12.Scer\UAS and SerScer\UAS.cSa fails to cause ectopic wings, expression of NScer\UAS.cBb with both wgl-12.Scer\UAS and SerScer\UAS.cSa causes ectopic wing development.