Allele Ecol\CAT^Adh.D33

General Information
Symbol	Ecol\CAT^Adh.D33	Species	E. coli
Name		FlyBase ID	FBal0040241
Feature type	allele	Associated gene	Ecol\CAT

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - deletion
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - deletion (England et al., 1990) in vitro construct - regulatory fusion (Jaynes and O'Farrell, 1988, Krasnow et al., 1989, Johnson and Krasnow, 1990, England et al., 1990, Jaynes and O'Farrell, 1991)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype	Ecol\CAT^Adh.D1320 (England et al., 1990)
Nature of the lesion	Statement Reference 31mer oligonucleotide encompassing the core of the 72bp copia enhancer sequence. (Cavarec et al., 1997) Ecol\CAT gene expression is driven by an Adh distal promoter fragment from -33 nucleotides. (Jaynes and O'Farrell, 1991) Construct: Ecol\CAT gene expression is driven by Adh distal promoter fragment from -33 to +53 relative to the start of distal transcription, Adf1 binding site is deleted. (England et al., 1990) Ecol\CAT expression is driven by an Adh fragment from -0.03kb to +0.05kb. (Krasnow et al., 1989) Ecol\CAT gene expression is driven by an Adh distal promoter fragment from -33 to +53 nucleotides. (Jaynes and O'Farrell, 1988)
Cytology
Phenotypic Data
Phenotypic Class

Phenotype Manifest In

Detailed Description
	Statement Reference
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

Comments
	Carried in plasmid transfected into Kc cells and used as a reporter plasmid in transcription activation assays. (Chen et al., 2000) Carried in plasmid "pD-33CAT", cotransfected into Schneider S2 cells with Dhyd\lola-like expression vector to identify the DNA-binding site sufficient to confer Dhyd\lola-like response to a heterologous basal promoter. (Cavarec et al., 1997) Carried in plasmid "pD-33CAT" and expressed in S2 cells. (Jaynes and O'Farrell, 1991) Carried in plasmid "pD-33CAT" and expressed in S2 cells. -33 deletion was incorrectly identified as a -34 in an earlier paper (FBrf0042068) due to a sequence reading error. (England et al., 1990) Carried in plasmid "pD-33CAT", cotransfected into Kc cells, and used as a reporter construct in transcriptional activation and repression assays with Ubx protein. (Johnson and Krasnow, 1990) Carried in plasmid pD-33CAT and assayed in S2 cells. (Krasnow et al., 1989) Carried in plasmid "pD-33CAT". (Jaynes and O'Farrell, 1988)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	CAT^Adh.D33 Ecol\CAT^Adh.D33
Name Synonym
Secondary FlyBase IDs

References ( 16 )
Research paper	Chen et al., 2000, Mol. Cell. Biol. 20(5): 1616--1625 Cubitus interruptus requires Drosophila CREB-binding protein to activate wingless expression in the Drosophila embryo. [FBrf0125204] Mailhos et al., 1998, Development 125(5): 937--947 Drosophila goosecoid requires a conserved heptapeptide for repression of paired-class homeoprotein activators. [FBrf0101950] Serrano and Maschat, 1998, EMBO J. 17(13): 3704--3713 Molecular mechanism of polyhomeotic activation by engrailed. [FBrf0103370] Akimaru et al., 1997, Nature 386(6626): 735--738 Drosophila CBP is a co-activator of cubitus interruptus in hedgehog signalling. [FBrf0093058] Cavarec et al., 1997, Mol. Cell. Biol. 17(1): 482--494 Molecular cloning and characterization of a transcription factor for the copia retrotransposon with homology to the BTB-containing Lola neurogenic factor. [FBrf0091372] Sheng et al., 1997, Biol. Chem. 378: 863--872 Modular organization of Pax/Homeodomain proteins in transcriptional regulation. [FBrf0100653] Fujioka et al., 1996, Genes Cells 1(8): 741--754 Runt domain partner proteins enhance DNA binding and transcriptional repression in cultured Drosophila cells. [FBrf0091381] Sauer and Jackle, 1993, Nature 364(6436): 454--457 Dimerization and the control of transcription by Kruppel. [FBrf0059156] Soeller et al., 1993, Mol. Cell. Biol. 13(12): 7961--7970 Isolation of cDNAs encoding the Drosophila GAGA transcription factor. [FBrf0064711] Wilson et al., 1993, Genes Dev. 7(11): 2120--2134 Cooperative dimerization of paired class homeo domains on DNA. [FBrf0064750] Jaynes and O'Farrell, 1991, EMBO J. 10: 1427--1433 Active repression of transcription by the engrailed homeodomain protein. [FBrf0053872] England et al., 1990, J. Biol. Chem. 265: 5086--5094 Purified Drosophila transcription factor, Adh distal factor-1 (Adf-1), binds to sites in several Drosophila promoters and activates transcription. [FBrf0052182] Johnson and Krasnow, 1990, Genes Dev. 4: 1044--1052 Stimulation of transcription by an Ultrabithorax protein in vitro. [FBrf0051820] Krasnow et al., 1989, Cell 57: 1031--1043 Transcriptional activation and repression by Ultrabithorax proteins in cultured Drosophila cells. [FBrf0049328] Jaynes and O'Farrell, 1988, Nature 336: 744--749 Activation and repression of transcription by homeodomain-containing proteins that bind a common site. [FBrf0048776]
Letter	Fitzpatrick et al., 1992, Nature 356(6370): 610--612 Homeodomain-independent activity of the fushi tarazu polypeptide in Drosophila embryos. [FBrf0057340]

Allele Ecol\CATAdh.D33

Allele Ecol\CAT^Adh.D33