|Symbol||Cbβ\DT-AAcp95EF.PK||Species||L. Corynephage beta|
|Feature type||allele||Associated gene||Cbβ\DT-A|
|Mutagen||in vitro construct - regulatory fusion|
What does this section display?
This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).
What does this section not display?
This section does not currently display links that were removed or gene model changes.
Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
|All updates||Click here to see a list of all updates to this record from FB2010_08 and on.|
|Nature of the Allele|
|Mutations Mapped to the Genome|
|Associated Sequence Data|
|Nature of the lesion|
|Carried in construct|
|Phenotype Manifest In|
Mutant males make no detectable accessory gland proteins in the main cells of their accessory glands (96% of the gland). They make secondary cell. ejaculatory bulb, and ejaculatory duct proteins.
Wild-type females mated to Cbβ\DT-AAcp95EF.PK males begin to deposit eggs only at 10 hours after mating and deposit 5.9 +/- 2 eggs over a 24 hour period, a level not significantly different from that seen for virgin females. The oocyte accumulation pattern in wild-type females mated to Cbβ\DT-AAcp95EF.PK males is similar to that of virgin females, although the number of oocytes at stages 8-9 and 10A-12 in these females is significantly increased compared to those found in virgin females.
Females mated to males expressing Cbβ\DT-AAcp95EF.PK have an autofluorescent mating plug in the right location. However, these mating plugs contain only the fluorescent posterior mating plug and do not contain the anterior non-autofluorescent mating plug.
Wild-type females mated to wild-type males and then remated with males carrying Cbβ\DT-AAcp95EF.PK have the same high egg hatch rate shortly after the remating as females remated with the wild-type males.
Males do not produce sperm or accessory gland main cell secretions. Females mated to these males are courted by males approximately half as vigorously as virgin females when tested 8 or 10 hours after the initial mating. When tested 24 hours after the initial mating, the attractiveness of the females is not significantly different from that of virgin females.
No detectable main-cell products in seminal fluid. They also lack sperm but produce secondary cell, ejaculatory duct and bulb products. Median life span of females receiving main-cell products from tud1 males is shorter than females receiving no main cell products from Cbβ\DT-AAcp95EF.PK males.
Males do not produce sperm. The egg-laying rate of females mated to Cbβ\DT-AAcp95EF.PK, who receive no sperm or female accessory gland main cell secretion is almost identical to that of unmated females. The majority of females are receptive to control males one day after previously mating.
|Phenotype Manifest In|
|Complementation & Rescue Data|
|Stocks ( 0 )|
|Notes on Origin|
Cbβ\DT-A in the main cells kills the cells by blocking protein translation. Microcauterised males are used as the controls for any difference in non-mating exposure to female survival.
|External Crossreferences & Linkouts|
|Synonyms & Secondary IDs ( 1 )|
|Secondary FlyBase IDs|
|References ( 13 )|