Allele Cbβ\Cbβ\DT-A^Acp95EF.PK

General Information
Symbol	Cbβ\DT-A^Acp95EF.PK	Species	L. Corynephage beta
Name		FlyBase ID	FBal0040451
Feature type	allele	Associated gene	Cbβ\DT-A

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Kalb et al., 1993, Chapman et al., 1995, Tram and Wolfner, 1998)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Coding sequences of Cbβ\DT-A are downstream of a main-cell promoter. (Chapman et al., 1995) Construct: 1.1kb Acp95EF upstream and transcribed sequences drive the expression of 3.1kb fragment of Ecol\lacZ. (Kalb et al., 1993)
Carried in construct	P{mc/DTA-E} (Lung and Wolfner, 2001, Kalb et al., 1993, Heifetz and Wolfner, 2004, Chapman et al., 1995, Lung et al., 2001, Lung and Wolfner, 2001, Prout and Clark, 2000, Chapman et al., 2000, Heifetz et al., 2001, Tram and Wolfner, 1998, Carvalho et al., 2006, Wong et al., 2008)
Cytology
Phenotypic Data
Phenotypic Class
	male sterile (Tram and Wolfner, 1998)
Phenotype Manifest In
	male accessory gland main cell (Heifetz and Wolfner, 2004) spermatozoon (Tram and Wolfner, 1998)
Detailed Description
	Statement Reference Mutant males make no detectable accessory gland proteins in the main cells of their accessory glands (96% of the gland). They make secondary cell. ejaculatory bulb, and ejaculatory duct proteins. (Heifetz and Wolfner, 2004) Wild-type females mated to Cbβ\DT-A^Acp95EF.PK males begin to deposit eggs only at 10 hours after mating and deposit 5.9 +/- 2 eggs over a 24 hour period, a level not significantly different from that seen for virgin females. The oocyte accumulation pattern in wild-type females mated to Cbβ\DT-A^Acp95EF.PK males is similar to that of virgin females, although the number of oocytes at stages 8-9 and 10A-12 in these females is significantly increased compared to those found in virgin females. (Heifetz et al., 2001) Females mated to males expressing Cbβ\DT-A^Acp95EF.PK have an autofluorescent mating plug in the right location. However, these mating plugs contain only the fluorescent posterior mating plug and do not contain the anterior non-autofluorescent mating plug. (Lung and Wolfner, 2001) Wild-type females mated to wild-type males and then remated with males carrying Cbβ\DT-A^Acp95EF.PK have the same high egg hatch rate shortly after the remating as females remated with the wild-type males. (Prout and Clark, 2000) Males do not produce sperm or accessory gland main cell secretions. Females mated to these males are courted by males approximately half as vigorously as virgin females when tested 8 or 10 hours after the initial mating. When tested 24 hours after the initial mating, the attractiveness of the females is not significantly different from that of virgin females. (Tram and Wolfner, 1998) No detectable main-cell products in seminal fluid. They also lack sperm but produce secondary cell, ejaculatory duct and bulb products. Median life span of females receiving main-cell products from tud¹ males is shorter than females receiving no main cell products from Cbβ\DT-A^Acp95EF.PK males. (Chapman et al., 1995) Males do not produce sperm. The egg-laying rate of females mated to Cbβ\DT-A^Acp95EF.PK, who receive no sperm or female accessory gland main cell secretion is almost identical to that of unmated females. The majority of females are receptive to control males one day after previously mating. (Kalb et al., 1993)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

Comments
	Cbβ\DT-A in the main cells kills the cells by blocking protein translation. Microcauterised males are used as the controls for any difference in non-mating exposure to female survival. (Chapman et al., 1995) Encodes the full strength Cbβ\DT-A. (Kalb et al., 1993)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	Cbβ\DT-A^Acp95EF.PK
Name Synonym
Secondary FlyBase IDs

References ( 13 )
Research paper	Wong et al., 2008, Genetics 180(2): 921--931 A role for Acp29AB, a predicted seminal fluid lectin, in female sperm storage in Drosophila melanogaster. [FBrf0207277] Carvalho et al., 2006, Curr. Biol. 16(7): 692--696 Allocrine modulation of feeding behavior by the Sex Peptide of Drosophila. [FBrf0190002] Heifetz and Wolfner, 2004, Proc. Natl. Acad. Sci. U.S.A. 101(16): 6261--6266 Mating, seminal fluid components, and sperm cause changes in vesicle release in the Drosophila female reproductive tract. [FBrf0175095] Heifetz et al., 2001, Proc. Biol. Sci. 268(1463): 175--180 Male contributions to egg production: the role of accessory gland products and sperm in Drosophila melanogaster. [FBrf0134832] Lung and Wolfner, 2001, Insect Biochem. Mol. Biol. 31(6-7): 543--551 Identification and characterization of the major Drosophila melanogaster mating plug protein. [FBrf0134681] Lung et al., 2001, J. Insect Physiol. 47(6): 617--622 Drosophila males transfer antibacterial proteins from their accessory gland and ejaculatory duct to their mates. [FBrf0134712] Chapman et al., 2000, Proc. Biol. Sci. 267(1448): 1097--1105 The role of male accessory gland protein Acp36DE in sperm competition in Drosophila melanogaster. [FBrf0128429] Prout and Clark, 2000, Proc. Biol. Sci. 267(1439): 201--203 Seminal fluid causes temporarily reduced egg hatch in previously mated females. [FBrf0125227] Tram and Wolfner, 1998, Proc. Natl. Acad. Sci. U.S.A. 95(7): 4051--4054 Seminal fluid regulation of female sexual attractiveness in Drosophila melanogaster. [FBrf0102019] Chapman et al., 1995, Nature 373(6511): 241--244 Cost of mating in Drosophila melanogaster females is mediated by male accessory gland products. [FBrf0079936] Kalb et al., 1993, Proc. Natl. Acad. Sci. U.S.A. 90(17): 8093--8097 Probing the function of Drosophila melanogaster accessory glands by directed cell ablation. [FBrf0059294]
Review	Chen, 1996, Experientia 52(6): 503--510 The accessory gland proteins in male Drosophila: structural, reproductive, and evolutionary aspects. [FBrf0089616]
Erratum	Lung and Wolfner, 2001, Insect Biochem. Mol. Biol. 32: 109 Identification and characterization of the major Drosophila melanogaster mating plug protein. [FBrf0151654]

Allele Cbβ\Cbβ\DT-AAcp95EF.PK

Allele Cbβ\Cbβ\DT-A^Acp95EF.PK