FlyBase Allele Report: Scer\GAL4[rho.PL]

General Information

Symbol

Scer\GAL4^rho.PL

Species

S. cerevisiae

Name

rhomboid promoter construct of Levine

FlyBase ID

FBal0040492

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

Carried in Construct

P{GAL4-ve.L}

Also Known As

rho-GAL4, ve-GAL4, rhoGAL4, rhomboid-Gal4

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Klaes et al., 1994)

Progenitor genotype

Carried in construct

P{GAL4-ve.L}

Cytology

Description

The rho promoter regulates expression of a GAL4 driver.

(Klaes et al., 1994)

Allele components

Component

Use(s)

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

No Assay Recorded

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

ventral midline of embryo

(Klaes et al., 1994)

ventral neurectoderm

(Klaes et al., 1994)

mesoderm

(Klaes et al., 1994)

distribution deduced from reporter

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 10

mesoderm

(Granderath et al., 2000)

ventral neurectoderm

(Granderath et al., 2000)

ventral midline of embryo

(Granderath et al., 2000)

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

ectoderm

(Golembo et al., 1996)

Additional Information

Statement

Reference

Drives expression in 8 cell rows to either side of the ventral midline in the embryonic ectoderm.

(FlyBase Curators, 2017)

In embryonic ectoderm, expression restricted to 8 cell rows on either side of the ventral midline.

(Schweitzer et al., 1995)

Marker for

Reflects expression of

rho

(Granderath et al., 2000, Schweitzer et al., 1995)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die during embryonic stage, with dpp^UAS.cSa

(Oh et al., 2002)

lethal - all die during embryonic stage, with net^UAS.cBa

(Brentrup et al., 2000)

some die during embryonic stage, with dpp^UAS.cSa

(Oh et al., 2002)

some die during embryonic stage, with net^UAS.cBa

(Brentrup et al., 2000)

wild-type, with Hem^UAS.Tag:MYC

(Bogdan and Klambt, 2003)

Phenotype Manifest In

central nervous system, with pnt^P1.UAS

(Klaes et al., 1994)

embryonic/first instar larval cuticle | ventral, with dpp^UAS.cSa

(Oh et al., 2002)

larval ventral nerve cord commissure, with shi^K44A.UAS

(Onel et al., 2004)

larval ventral nerve cord commissure | embryonic stage, with Arf6^T27N.UAS

(Onel et al., 2004)

symmetrical commissure, with pnt^P1.UAS

(Klaes et al., 1994)

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressor of

Statement

Reference

Scer\GAL4^rho.PL/shi^K44A.UAS is a suppressor of larval ventral nerve cord commissure phenotype of sli⁵⁵⁰

(Onel et al., 2004)

NOT Suppressor of

Statement

Reference

Scer\GAL4^rho.PL/ac^UAS.cPa is a non-suppressor of ventral neurectoderm medial zone phenotype of vnd^D38

(Chu et al., 1998)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^rho.PL/Hem^{UAS.Tag:Myr(Src64B)} rescues Hem^C3-20

(Bogdan and Klambt, 2003)

Scer\GAL4^rho.PL/glec^UAS.cSa rescues Df(3R)e-BS2/glec^m98

(Sharrow and Tiemeyer, 2001)

Scer\GAL4^rho.PL/glec^UAS.cSa rescues Df(3R)e-BS2/glec^m24

(Sharrow and Tiemeyer, 2001)

Partially rescues

Scer\GAL4^rho.PL/glec^UAS.cSa partially rescues Df(3R)e-BS2

(Sharrow and Tiemeyer, 2001)

Scer\GAL4^rho.PL/vnd^UAS.cCa partially rescues vnd^D38

(Chu et al., 1998)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

26871

w^*; P{GAL4-ve.L}MG3; TM2/TM6B, Tb¹

Notes on Origin

Discoverer

Comments

Generated in M. Levine's lab.

(Klämbt, 1995.8.1)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

GAL4^ve.PL

Scer\GAL4^rho.PL

Scer\GAL4^ve.PL

Name Synonyms

rhomboid promoter construct of Levine

Secondary FlyBase IDs

References (34)

Report Sections

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