FB2025_01 , released February 20, 2025
Allele: Ecol\lacZdpp.BS3.0
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General Information
Symbol
Ecol\lacZdpp.BS3.0
Species
E. coli
Name
FlyBase ID
FBal0041158
Feature type
allele
Associated gene
Ecol\lacZ
Associated Insertion(s)
Carried in Construct
P{BS3.0}
Also Known As
dpp-lacZ, dpp-lacZBS3.0, dpp-lacZ BS3.0, BS3.0, dppZ, dpp-lacZ (BS3.0), dpp-lacZ(BS3.0), dpp:lacZ
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Freeland and Kuhn, 1996, Buratovich and Bryant, 1995, Capdevila et al., 1994, Kojima et al., 1994, Heberlein et al., 1993, Blackman et al., 1991, Raftery et al., 1991)
Progenitor genotype
Ecol\lacZHsp70Bb.-194
(Blackman et al., 1991)
Carried in construct
P{BS3.0}
Cytology
Description

lacZ expression is controlled by the 3' dpp enhancer.

(Buratovich and Bryant, 1995)

A dpp disc enhancer drives lacZ expression.

(Kojima et al., 1994)

10kb EcoRI fragment spanning dpp region disk III--II boundary drives lacZ expression.

(Heberlein et al., 1993)

12kb EcoRI fragment spanning dpp region disk III--II boundary drives lacZ expression.

(Blackman et al., 1991)

lacZ expression is driven by fragment from 106.9-116.9kb of dpp, at the disk III-disk II boundary.

(Raftery et al., 1991)
Allele components
Component
Use(s)
Regulatory region(s)
dpp_BS3.0
Encoded product / tool
lacZ
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      No Assay Recorded
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      embryonic stage 15
      procephalic segment
      (Blackman et al., 1991)
      first instar larval stage
      imaginal disc primordium
      (Blackman et al., 1991)
      third instar larval stage
      male genital disc | restricted
      (Blackman et al., 1991)
      eye-antennal disc | restricted
      (Khalsa et al., 1998)
      larval brain | restricted
      (Blackman et al., 1991)
      leg disc | restricted
      (Blackman et al., 1991)
      distribution deduced from reporter
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      embryonic stage 15
      embryonic brain
      (Blackman et al., 1991)
      thoracic segment
      (Blackman et al., 1991)
      larval stage
      eye disc morphogenetic furrow
      (Mukherjee et al., 2000)
      eye-antennal disc | restricted
      (Mukherjee et al., 2000)
      anterior-posterior compartment boundary of the leg disc
      (Mukherjee et al., 2000)
      first instar larval stage
      embryonic/larval labral segment sense organ
      (Blackman et al., 1991)
      third instar larval stage
      eye disc | restricted
      (Hazelett et al., 1998)
      leg disc | restricted
      (Khalsa et al., 1998)
      female genital disc | restricted
      (Gorfinkiel et al., 1999, Freeland and Kuhn, 1996, Blackman et al., 1991)
      male genital primordium (female)
      (Gorfinkiel et al., 1999)
      male genital disc | restricted
      (Gorfinkiel et al., 1999, Freeland and Kuhn, 1996)
      wing disc | restricted
      (Khalsa et al., 1998, Capdevila and Guerrero, 1994, Blackman et al., 1991)
      eye-antennal disc | restricted
      (Chauhan et al., 2013, Blackman et al., 1991)
      eye disc morphogenetic furrow
      (Epps et al., 1997)
      anterior-posterior compartment boundary of the wing disc
      (Capdevila and Guerrero, 1994)
      anterior-posterior compartment boundary of the wing disc | restricted
      (Chauhan et al., 2013)
      early third instar larval stage
      eye disc | lateral | posterior
      (Li et al., 2013)
      distribution deduced from reporter (Gal4 UAS)
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      wandering third instar larval stage
      anterior-posterior compartment boundary of the wing disc
      (Simmonds et al., 1995)
      immunolocalization
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      third instar larval stage
      eye disc morphogenetic furrow
      (Vrailas and Moses, 2006)
      Additional Information
      Statement
      Reference

      The dpp reporter is mostly expressed anterior to phosphorylated Mad at the morphogenetic furrow in third instar larval eye discs.

      (Vrailas and Moses, 2006)

      Expression is observed in both the male and female genital discs, and is complementary to that of wg. Expression is excluded from the repressed male primordium in the female genital disc.

      (Gorfinkiel et al., 1999)

      The EcollacZdpp.BS3.0 reporter construct reflects wildtype expression of dpp in the imaginal discs. Expression is detected in a stripe along the anterior-posterior boundary of the wing disc and along the morphogenetic furrow in the eye-antennal disc.

      (Khalsa et al., 1998)

      Expressed in cells on anterior side of the A/P boundary.

      (Capdevila and Guerrero, 1994)

      The expression pattern of EcollacZdpp.BS3.0 most closely recapitulates wild-type dpp expression patterns during development.

      (Blackman et al., 1991)
       
      Marker for
      anterior-posterior compartment boundary of the wing disc
      (Simmonds et al., 1995)
      female genital disc
      (Gorfinkiel et al., 1999)
      male genital disc
      (Gorfinkiel et al., 1999)
      Reflects expression of
      dpp
      (Chauhan et al., 2013, Li et al., 2013, Vrailas and Moses, 2006, Mukherjee et al., 2000, Gorfinkiel et al., 1999, Hazelett et al., 1998, Khalsa et al., 1998, Freeland and Kuhn, 1996, Simmonds et al., 1995, Blackman et al., 1991)
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (4)
      Notes on Origin
      Discoverer

      Blackman

      (Capdevila et al., 1994)
      Comments
      Comments

      Carried in a plasmid, expressed in cl-8 cells and used as a reporter construct to study response to hh signalling in these cells.

      (Chen et al., 1999)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (5)
      Reported As
      Symbol Synonym
      BS3.0
      (Chen et al., 1999)
      Ecol\lacZdpp.BS3.0
      dpp-lacZ
      (Apionishev et al., 2005)
      dppBS3.0
      (Baonza et al., 2000)
      lacZdpp.BS3.0
      Name Synonyms
      Secondary FlyBase IDs
        References (262)