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General Information
Symbol
Ecol\lacZDptA.PR
Species
E. coli
Name
Diptericin promoter construct of Reichhart
FlyBase ID
FBal0041171
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
Dpt-lacZ, Dipt-lacZ, Diptericin-lacZ, Pdipt-lacZ
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

lacZ expression is driven by 2.2kb DptA promoter region.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
distribution deduced from reporter
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Information
Statement
Reference

Reporter gene expression is induced inresponse to injection of complete Freund's adjuvant in late third instarlarvae (96-120hr). Younger animals do not express the reporter gene inresponse to the injection. From pupariation on, there is a gradualdecrease in the response to the challenge. In response to live bacteria, afaint response is seen in second instar larvae and a full response is seenin adults.

 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Adults are pricked with a sterile needle dipped in culture pellets of various living microorganisms (distinct bacterial strains, fungal spores or hyphae). The level of induction of the reporter gene varies with the microorganism tested. Pricking results in a low but clearly detectable expression of all antimicrobial genes and these genes are induced above the background level by specific classes of microorganism.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (9)
Notes on Origin
Discoverer
Comments
Comments

Carried in a plasmid, transformed into l(2)mbn cells and induction of the promoter by ecdysone treatment is assayed using Ecol\lacZ activity levels. Gram negative bacteria are the best inducers of Dpt gene expression in ecdysone-treated l(2)mbn cells.

Stably transfected into S2 cells and used as a reporter for imd pathway activity.

Strong induction of Ecol\lacZ gene expression after bacterial challenge.

Inducible expression is superimposed on that of the endogenous Dpt gene at the end of the third larval instar.

Induction of the transgene by immune challenge parallels that of the resident Dpt gene.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (6)
Reported As
Symbol Synonym
Ecol\lacZDpt.P
Ecol\lacZDpt.PR
Ecol\lacZDptA.PR
lacZDpt.P
lacZDpt.PR
Name Synonyms
Diptericin promoter construct of Reichhart
Secondary FlyBase IDs
  • FBal0043850
References (60)