FlyBase Allele Report: Ecol\lacZ[vg.int2.1]

General Information

Symbol

Ecol\lacZ^vg.int2.1

Species

E. coli

Name

FlyBase ID

FBal0042236

Feature type

allele

Associated gene

Ecol\lacZ

Associated Insertion(s)

Carried in Construct

P{vg(D/V)-lacZ}

Also Known As

vg^BE-lacZ, vgBE-lacZ, vgBE, vg-lacZ, vg-BE, vg BE-lacZ, vg-BE-lacZ, vgB-lacZ

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Kim et al., 1996, Williams et al., 1994)

Progenitor genotype

Carried in construct

P{vg(D/V)-lacZ}

Cytology

Description

lacZ expression is governed by the wild-type vg second intron D/V boundary enhancer element.

(Kim et al., 1996)

lacZ gene expression is driven by a 750bp EcoRI fragment from vg intron 2 (the vg intron 2 enhancer element) that shares sequence homology with the same fragment from Dvir\vg.

(Williams et al., 1994)

Allele components

Component

Use(s)

Regulatory region(s)

vg_boundary_enhancer

Encoded product / tool

lacZ

reporter enzyme

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter

Stage

Tissue/Position (including subcellular localization)

Reference

second instar larval stage

wing disc | restricted

(Williams et al., 1994)

third instar larval stage

haltere disc | restricted

(Williams et al., 1994)

wing disc | restricted

(Williams et al., 1994)

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

second instar larval stage -- third instar larval stage

dorsal-ventral compartment boundary of the wing disc

(Neumann and Cohen, 1996)

anterior-posterior compartment boundary of the wing disc of wing pouch

(Neumann and Cohen, 1996)

Additional Information

Statement

Reference

EcollacZ^vg.int2.1 transcript is expressed starting at the beginning of the second larval instar in the wing disc. EcollacZ^vg.int2.1 expression is observed along the D/V boundary of the wing disc, as well as along the part of the A/P boundary that lies within the wing pouch.

(Neumann and Cohen, 1996)

Expression restricted to a stripe of cells spanning the dorsal/ventral boundary in the wing and haltere discs. No expression observed in any other larval or embryonic tissues. Earliest expression in second instar wing disc is weak and diffuse; by late second instar has resolved into a broad stripe along the D/V boundary.

(Williams et al., 1994)

Marker for

Reflects expression of

(Neumann and Cohen, 1996)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Ecol\lacZ^vg.int2.1

lacZ^vg.int2.1

vgBE

(Klein and Martinez Arias, 1998)

Name Synonyms

Secondary FlyBase IDs

FBal0055751

References (70)

Report Sections

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