Allele Dmel\Eip74EF^DL-1

General Information
Symbol	Dmel\Eip74EF^DL-1	Species	D. melanogaster
Name		FlyBase ID	FBal0042541
Feature type	allele	Created / Updated	2006-08-22/2006-08-22
Associated gene	Dmel\Eip74EF

Allele class	loss of function
Mutagen	ethyl methanesulfonate
Nature of the Allele
Allele class	loss of function (Fletcher and Thummel, 1995, Fletcher and Thummel, 1995)
Mutagen	ethyl methanesulfonate (Fletcher et al., 1995)
Mapped Features and Mutations
Type Symbol & Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference 14bp deletion in the E74B-specific exon that leads to a premature stop codon. (Fletcher and Thummel, 1995) Putative E74B mutation, 14bp deletion from position +1118 to +1131 relative to the E74B transcriptional start site. The deletion alters the reading frame such that a stop codon is introduced 114bp downstream of the deletion prematurely terminating the open reading frame in the middle of the first exon. (Fletcher et al., 1995)
Assay mode
Cytology
Phenotypic Data
Phenotypic Class
	lethal \| recessive (Fletcher and Thummel, 1995) lethal \| larval stage (Fletcher et al., 1995)
Phenotype Manifest In
	air bubble (Fletcher et al., 1995) anterior pupal spiracle (Fletcher et al., 1995) procephalic segment (Fletcher et al., 1995) larval midgut & prepupa (with Df(3L)81k19) (Lee et al., 2002)
Detailed Description
	Statement Reference Lethal when carried as a transheterozygote with other Eip74EF mutations, hemizygotes are pupal lethal. Some hemizygous progeny arrest prior to pupation, they have a gas bubble and have partially undergone larval-pupal apolysis. Others undertake but fail to complete pupariation, the gas bubble is expelled into the posterior end, but fails to translocate the gas pocket anteriorly so the gas remains trapped between the hypodermis and pupal case. Remaining hemizygous pupae fail to completely evert their anterior spiracles and cephalic complexes and they retain their long segmented larval shapes, tanning proceeds normally but the darkened cuticle is soft. A few succeed in everting the cephalic complex but do not display further head development, the microcephalic phenotype. (Fletcher et al., 1995) Homozygous mutant γ neurons (generated in the brain as somatic clones) exhibit no detectable abnormalities in larval or adult stages. (Lee et al., 2000) At the onset of pupation, as staged by head eversion, Eip74EF^DL-1/Df(3L)81k19 mutant animals exhibit the following phenotypes: 1. Mutant larval midguts are extremely condensed and the adult and larval epithelia have separated such that a defined adult lumen exists. These mutants also form an adult midgut epithelium, and the larval proventriculus and gastric caeca are destroyed. Thus, Eip74EF^neo24/Df(3L)81k19 larval midguts appear to be arrested at a stage of destruction that is similar to the midgut of wild-type animals 6-12 h following puparium formation. 2. DNA fragmentation is detected in all larval midgut cells of mutant animals 3. Mutant larval midgut cells appear to die normally and have numerous autophagic structures, including mitochondria in vacuoles, crystalline inclusions, and myelin-like membrane swirls. Between the white prepua stage and head eversion, Eip74EF^DL-1/Df(3L)81k19 mutant midguts decrease in length by an average of 73% in length compared to 85% in the wild-type. (Lee et al., 2002)
Interactions
Phenotypic Class
Other
Statement Reference Eip74EF^DL-1, br^2Bc-2 has lethal \| prepupal stage \| recessive phenotype (Fletcher and Thummel, 1995, Fletcher and Thummel, 1995) Eip74EF^DL-1, br⁵ has lethal \| larval stage \| recessive phenotype (Fletcher and Thummel, 1995, Fletcher and Thummel, 1995)
Phenotype Manifest In
NOT Enhancer of
Statement Reference Eip74EF^DL-1 is a non-enhancer of phenotype of zip^Ebr (Halsell and Kiehart, 1998)
Additional Comments
Genetic Interactions
Statement Reference Double heterozygotes with br^rbp-5, br⁵ or br^2Bc-2 show no effect on development. Proportion of Eip74EF^DL-1 flies showing either a prepupal or pharate lethal phenotype is unchanged as the effective dose of br⁺ is reduced. All br⁵, Eip74EF^DL-1 double mutants arrest development at the larval/prepupal transition, whereas Eip74EF^DL-1 mutants show a range of lethal phases from prepupal to pharate adult. In double mutants with br^2Bc-2 the lethal phase shifts earlier to the prepupal stage, and the puparium is extremely curved. (Fletcher and Thummel, 1995)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 2 )
Bloomington	4435 Eip74EF^DL-1 st¹ p^p e¹¹/TM6C, Sb¹ Tb¹
Kyoto	107745 Eip74EF^DL-1 st¹ p^p e¹¹/TM6C, Sb¹ Tb¹
Notes on Origin
Discoverer	Burtis, 1990.

Comments
	Puffs normally active at puparium formation are prematurely induced in hemizygous white prepupae. (Fletcher et al., 1995) Retains little or no E74B function while leaving the E74A function unaffected. (Fletcher and Thummel, 1995)
Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	E74^DL-1 (Lee et al., 2000, Halsell and Kiehart, 1998, Fletcher and Thummel, 1995, Fletcher et al., 1995) E74B^DL-1 (Lee et al., 2002) Eip74EF^DL-1
Name Synonym
Secondary FlyBase IDs

References ( 7 )
Research paper	Lee et al., 2002, Dev. Biol. 250(1): 101--111 Steroid regulation of midgut cell death during Drosophila development. [FBrf0151883] Lee et al., 2000, Neuron 28(3): 807--818 Cell-autonomous requirement of the USP/EcR-B ecdysone receptor for mushroom body neuronal remodeling in Drosophila. [FBrf0132434] Halsell and Kiehart, 1998, Genetics 148(4): 1845--1863 Second-site noncomplementation identifies genomic regions required for Drosophila nonmuscle myosin function during morphogenesis. [FBrf0102325] Fletcher and Thummel, 1995, Genetics 141(3): 1025--1035 The ecdysone-inducible Broad-Complex and E74 early genes interact to regulate target gene transcription and Drosophila metamorphosis. [FBrf0083928] Fletcher et al., 1995, Development 121(5): 1455--1465 The Drosophila E74 gene is required for metamorphosis and plays a role in the polytene chromosome puffing response to ecdysone. [FBrf0078417] Fletcher and Thummel, 1995, Development 121(5): 1411--1421 The Drosophila E74 gene is required for the proper stage- and tissue-specific transcription of ecdysone-regulated genes at the onset of metamorphosis. [FBrf0078416]
Personal communication to FlyBase	Deal and Cook, 2001.12.6, Isolation and characterization of Df(3L)BSC8. Isolation and characterization of Df(3L)BSC8. [FBrf0141806]

Allele Dmel\Eip74EFDL-1

Allele Dmel\Eip74EF^DL-1