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General Information
Symbol
Mmus\Prkacahs.PJ
Species
M. musculus
Name
heat shock construct of Jiang
FlyBase ID
FBal0042694
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
hsPKA, hsp70-PKA*, hs-PKA*
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

The coding sequence of the catalytic subunit of Mmus\Prkaca with amino acid substitutions: H87Q and W196R. Expression of the mutated subunit is driven by an Hsp70 promoter.

Allele components
Product class / Tool use(s)
Regulatory region(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Mmus\Pkacahs.PJ-overexpressing flies exhibit extended life spans.

The presence of Mmus\Pkacahs.PJ does not affect fly survival rates.

Some flies expressing Mmus\Pkacahs.PJ are arrhythmic with respect to locomotor activity (only 73.33% are rhythmic).

Some flies expressing Mmus\Pkacahs.PJ are arrhythmic with respect to locomotor activity.

Overexpression of Mmus\Pkacahs.PJ in a wild-type background does not increase learning score, even if the flies are undertrained.

Animals carrying two copies of Mmus\Pkacahs.PJ are subviable although survivors appear morphologically normal. Basal expression of Mmus\Pkacahs.PJ is sufficient to suppress ectopic dpp expression. Complete rescue is observed when larvae are shifted to 33oC.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhancer of
Statement
Reference

Mmus\Prkacahs.PJ is an enhancer of locomotor rhythm defective | heat sensitive phenotype of Nf1P2

Mmus\Prkacahs.PJ is an enhancer of oxidative stress response defective | heat sensitive phenotype of Nf1P2

Suppressor of
NOT Suppressor of
Statement
Reference
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference

The presence of the heat shock-inducible and constitutively active Mmus\Pkacahs.PJ suppresses the reduction in life span seen in Nf1P2 mutants.

Mmus\Pkacahs.PJ increases the survival rate of flies that express Hsap\HDQ48.ex1p.Scer\UAS in the nervous system at 18[o]C and 22[o]C (under the regulation of Scer\GAL4elav-C155). The increase in survival rate with Mmus\Pkacahs.PJ is larger at 22[o]C than at 18[o]C (~10% at 22[o]C and ~5% at 18[o]C).

Mmus\Pkacahs.PJ completely rescues the learning defect of Nf1P1 when the flies are raised at room temperature. Mmus\Pkacahs.PJ partially rescues the learning defect of Nf1P2 when the flies are raised at room temperature, and completely rescues Nf1P2 when it is expressed using heat shock (37oC, 30 minutes). Nf1P1 and Nf1P2 flies show a short-term memory defect (3 and 8 hour retention) which is completely rescued by heat shock induced expression of Mmus\Pkacahs.PJ.

Weak heat induced expression at 28oC causes significant rescue of the Nf1 mutant pupal phenotype. Higher expression levels cause lethality.

Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
Comments
Comments

Mutated form of the catayltic subunit does not bind the regulatory subunit with high affinity and hence is constitutively active, irrespective of cAMP concentration.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
Mmus\Pkacahs.PJ
Mmus\Prkacahs.PJ
PKAhs.PJ
Name Synonyms
heat shock construct of Jiang
Secondary FlyBase IDs
    References (10)