FlyBase Allele Report: Dmel\Pka-C1[E95]

General Information

Symbol

Dmel\Pka-C1^E95

Species

D. melanogaster

Name

FlyBase ID

FBal0042698

Feature type

allele

Associated gene

Dmel\Pka-C1

Associated Insertion(s)

Carried in Construct

Also Known As

DC0^E95, DCO^E95, PKA^E95

Key Links

Allele class

loss of function allele

amorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Chen and Struhl, 1996, Jiang and Struhl, 1995)

loss of function allele

(Struhl et al., 1997)

Mutagen

ethyl methanesulfonate

(Jiang and Struhl, 1995)

Progenitor genotype

Cytology

Description

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased cell death | somatic clone

(Kimura et al., 2004)

lethal | recessive

(Jiang and Struhl, 1995)

visible

(Wehrli and Tomlinson, 1995)

visible | cell non-autonomous | recessive | somatic clone

(Phillips et al., 1999)

visible | recessive | somatic clone

(Phillips et al., 1999, Wang et al., 1999)

Phenotype Manifest In

adult abdominal segment anterior compartment

(Struhl et al., 1997)

cuticle

(Struhl et al., 1997)

eye photoreceptor cell

(Wehrli and Tomlinson, 1995)

leg | ectopic | somatic clone

(Jiang and Struhl, 1995)

macrochaeta

(Struhl et al., 1997)

macrochaeta | cell non-autonomous | somatic clone

(Phillips et al., 1999)

macrochaeta | somatic clone

(Phillips et al., 1999)

mesothoracic tergum & macrochaeta | somatic clone | cell non-autonomous

(Phillips et al., 1999)

microchaeta

(Struhl et al., 1997)

ommatidium

(Wehrli and Tomlinson, 1995)

scutum | somatic clone

(Phillips et al., 1999)

wing | ectopic | somatic clone

(Jiang and Struhl, 1995)

wing | somatic clone

(Kimura et al., 2004, Wang et al., 1999)

Detailed Description

Statement

Reference

Somatic clones of Pka-C1^E95 homozygous cells in the posterior compartment of the adult wing blade persist after surrounding heterozygous or wild-type cells have died.

(Kimura et al., 2004)

Homozygous clones in the notum induce formation of macrochaetae in adjacent wild-type cells. Only wild-type cells on or lateral to the dorsocentral (DC) meridian respond to the nearby presence of a homozygous clone by making extra macrochaetae. Most clones in the scutum lateral or medial to the DC meridian form pits, invaginations into the cuticle surface. In the medial prescutum, most homozygous clones form normal patterns containing only microchaetae and these clones do not contain macrochaetae.

(Phillips et al., 1999)

Homozygous clones in the wing induce the formation of supernumerary wings. Within the supernumerary wing, the homozygous cells contribute to intervein tissue that normally forms posteriorly to vein 3.

(Wang et al., 1999)

Clones in the anterior compartment of the adult abdomen develop normally or almost normally when they arise near the anterior/posterior compartment boundary. Clones that arise more anteriorly in the a3 and a4 regions, form cuticle characteristic of a more posterior position (a5) within the anterior compartment, and the hairs and bristles produced are more crowded than in the surrounding wild-type tissue. This transformation is autonomous. The polarity of hairs and bristles both within and outside the clone is abnormal. Anterior clones are under-represented. Clones that are doubly mutant for Pka-C1^E95 and wg^l-17, or triply mutant for Pka-C1^E95, wg^l-17 and dpp^unspecified have the same phenotype as single mutant Pka-C1^E95 clones. Clones that arise in the a2 anterior region of the anterior compartment are transformed to make naked a1 cuticle.

(Struhl et al., 1997)

Homozygous mutant clones in the wing produce supernumerary double anterior wings and in the leg anterior clones both ventrally and dorsally cause supernumerary double anterior hindlegs.

(Jiang and Struhl, 1995)

Ommatidia within a clone are invariably mutant, ommatidia may have extra or less photoreceptor cells, appear to be of either chiral form and in a random orientation. Ommatidia in a region posterior to a clone are of the inappropriate chiral form but they are still mirror reflection either side of the equator. This presumable reflects the backward flow of the ectopic wave before it meets the anteriorly advancing endogenous wave. Ommatidia anterior to the clones have the correct chirality and orientation as the morphogenetic ectopic wave is moving in the correct posterior to anterior direction. It is unclear of ommatidial chirality is perturbed in regions where the morphogenetic wave flows perpendicular to the normal direction.

(Wehrli and Tomlinson, 1995)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Pka-C1^E95 has visible | recessive phenotype, suppressible by dpp^H61

(Phillips et al., 1999)

NOT suppressed by

Statement

Reference

Pka-C1^E95 has abnormal planar polarity | somatic clone phenotype, non-suppressible by Df(2L)RF

(Lawrence et al., 2002)

Suppressor of

Statement

Reference

Pka-C1[+]/Pka-C1^E95 is a suppressor of partially lethal - majority die | maternal effect phenotype of Pka-R1¹⁸³⁰⁴

(Yoshida et al., 2004)

Other

Statement

Reference

Pka-C1^E95, sgg³² has visible | recessive phenotype

(Phillips et al., 1999)

Phenotype Manifest In

Suppressed by

Statement

Reference

Pka-C1^E95, smo³ has wing disc | ectopic phenotype, suppressible by sgg^M11

(Price and Kalderon, 2002)

Pka-C1^E95 has macrochaeta | ectopic | somatic clone | cell non-autonomous phenotype, suppressible by dpp^H61

(Phillips et al., 1999)

NOT suppressed by

Statement

Reference

Pka-C1^E95 has adult cuticle & abdomen | somatic clone phenotype, non-suppressible by Df(2L)RF

(Lawrence et al., 2002)

Pka-C1^E95, smo³ has mesonotum | ectopic phenotype, non-suppressible by sgg^M11

(Price and Kalderon, 2002)

Suppressor of

Statement

Reference

Pka-C1[+]/Pka-C1^E95 is a suppressor of embryonic abdominal segment | germline clone | maternal effect phenotype of Pka-R1^E1/Pka-R1¹⁸³⁰⁴

(Yoshida et al., 2004)

Pka-C1[+]/Pka-C1^E95 is a suppressor of embryonic head | germline clone | maternal effect phenotype of Pka-R1^E1/Pka-R1¹⁸³⁰⁴

(Yoshida et al., 2004)

Other

Statement

Reference

Pka-C1^E95, smo³ has mesonotum | ectopic phenotype

(Price and Kalderon, 2002)

Pka-C1^E95, smo³ has wing disc | ectopic phenotype

(Price and Kalderon, 2002)

Pka-C1^E95, sgg³² has prescutum phenotype

(Phillips et al., 1999)

Pka-C1^E95, sgg³² has scutum | anterior phenotype

(Phillips et al., 1999)

Additional Comments

Genetic Interactions

Statement

Reference

When Pka-C1^E95, Df(2L)RF (which removes the genes Wnt4, Wnt6 and Wnt10) homozygous clones are made in the adult abdomen, the polarity is reversed in the same way as Pka-C1^E95 clones are.

(Lawrence et al., 2002)

Pka-C1^E95, sgg^M11 smo³ triple mutant clones in the wing disc exhibit some outgrowths from the notum but no significant wing pattern duplications. Wing discs with Pka-C1^E95, smo³ clones have large expansions affecting the anterior compartment of the wing disc.

(Price and Kalderon, 2002)

The non-autonomous extra macrochaetae phenotype of homozygous clones is suppressed if the clones are also homozygous for dpp^H61. Unlike Pka-C1^E95 clones, sgg³² Pka-C1^E95 double mutant clones in the medial prescutum produce macrochaetae autonomously, and unlike sgg³² clones, the double mutant clones have a symmetrical bristle pattern. In the posterior scutum, sgg³² Pka-C1^E95 double mutant clones have a scutellar phenotype very similar to that of Pka-C1^E95 clones. In the anterior scutum, sgg³² Pka-C1^E95 double mutant clones no longer show the non-autonomous induction of macrochaetae or formation of pits characteristic of Pka-C1^E95 clones. Formation of macrochaetae within the double mutant clones is increased relative to sgg³² clones.

(Phillips et al., 1999)

Clones double mutant for dpp^H61 Pka-C1^E95 do not cause supernumerary structures in the leg and wing. Clones positioned at the compartment boundary exhibit long range effects causing a marked reduction in wing size and loss of vein pattern in anterior and posterior compartments. Short range effects include inducing immediate neighbours to form ectopic veins and margin bristles, the clones fail to differentiate wing veins in a cell autonomous fashion. dpp^d12 Pka-C1^E95 also fail to form veins in the anterior compartment. wg^l-17 Pka-C1^E95 clones cause local outgrowths of leg tissue and on rare occasions form of supernumerary legs.

(Jiang and Struhl, 1995)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Global rearrangements of the wing and leg occur as the clones express dpp.

(Jiang and Struhl, 1995)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

DC0^E95

(Xia et al., 2012, Jia et al., 2004, Wang et al., 1999, Chen and Struhl, 1996, Jiang and Struhl, 1996, Wehrli and Tomlinson, 1995)

DCO^E95

(Kimura et al., 2004, Yoshida et al., 2004, Methot and Basler, 2000)

PKA^E95

(Methot and Basler, 2000, Phillips et al., 1999)

Pka-C1^E95

Pka^-

(Lawrence et al., 2002)

Pka^E95

(Tanimoto et al., 2000)

pka-C1^E95

(Vegh and Basler, 2003)

Name Synonyms

Secondary FlyBase IDs

References (16)

Report Sections

Open Close