Allele Dmel\Ras85D⁰⁵⁷⁰³

General Information
Symbol	Dmel\Ras85D⁰⁵⁷⁰³	Species	D. melanogaster
Name		FlyBase ID	FBal0042723
Feature type	allele	Associated gene	Dmel\Ras85D
Also Known As	Ras1⁵⁷⁰³, ras⁵⁷⁰³

Map ( GBrowse )	Untitled Document
Allele class	hypomorphic allele - genetic evidence
Mutagen	P-element activity
Recent Updates
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Nature of the Allele
Allele class	hypomorphic allele - genetic evidence (Rorth, 1996, Schnorr and Berg, 1996)
Mutagen	P-element activity (Rorth, 1996, Schnorr and Berg, 1996)
Mutations Mapped to the Genome

Type Location Additional Notes References transposable element insertion site 3R:5,338,761..5,338,761 (Rorth, 1996, BDGP Project Members, 1994-1999, Schnorr and Berg, 1996)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference P{PZ} insertion is located 28bp upstream of a putative transcription start site as determined by cDNA analysis. (Schnorr and Berg, 1996)
Caused by insertion	P{PZ}Ras85D⁰⁵⁷⁰³ (Rorth, 1996, BDGP Project Members, 1994-1999, Schnorr and Berg, 1996)
Cytology
Phenotypic Data
Phenotypic Class
	female sterile (Schnorr and Berg, 1996) female sterile \| recessive (Rorth, 1996) neuroanatomy defective (Koh et al., 2002) partially lethal - majority die (Schnorr and Berg, 1996) partially lethal - majority die \| recessive (Rorth, 1996) visible (Schnorr and Berg, 1996)
Phenotype Manifest In
	abdominal segment 1 (Schnorr and Berg, 1996) abdominal segment 2 (Schnorr and Berg, 1996) abdominal segment 3 (Schnorr and Berg, 1996) abdominal segment 4 (Schnorr and Berg, 1996) abdominal segment 5 (Schnorr and Berg, 1996) abdominal segment 6 (Schnorr and Berg, 1996) abdominal segment 7 (Schnorr and Berg, 1996) abdominal segment 8 (Schnorr and Berg, 1996) abdominal segment 9 (Schnorr and Berg, 1996) abdominal segment 10 (Schnorr and Berg, 1996) abdominal segment 11 (Schnorr and Berg, 1996) chorion (Schnorr and Berg, 1996) dorsal appendage (Schnorr and Berg, 1996) dorsal appendage \| maternal effect (with Ras85D^e1B) (Ward and Berg, 2005) egg chamber (Schnorr and Berg, 1996) embryonic/first instar larval cuticle (Schnorr and Berg, 1996) female terminalia (Schnorr and Berg, 1996) head (Schnorr and Berg, 1996) male terminalia (Schnorr and Berg, 1996) nurse cell (Schnorr and Berg, 1996) ommatidium (Schnorr and Berg, 1996) oocyte (Schnorr and Berg, 1996) ovary (Schnorr and Berg, 1996) type I bouton (Koh et al., 2002)
Detailed Description
	Statement Reference 100% of the eggs laid by Ras85D⁰⁵⁷⁰³/Ras85D^e1B mothers have a single central dorsal appendage. (Ward and Berg, 2005) The Ras85D⁰⁵⁷⁰³ mutant result in a significant reduction in the number of type I synaptic boutons compared with wild-type. (Koh et al., 2002) Homozygotes have no detectable eye defects. (Rorth, 1996) A low percentage of egg chambers from homozygous females degenerate between stages 8 and 10. Some egg chambers contain too few nurse cells or fail to correctly position the developing oocyte within the egg chamber. Female transheterozygotes with Df(3R)by10 have rudimentary ovaries. All late stage egg chambers lack dorsal appendage structures and possess only a patch of chorion centered on the dorsal midline. Few eggs are laid, eggs appear ventralised but their overall shape is normal. Homozygous eyes exhibit slightly disrupted ommatidial array. Ras85D^ΔC17b/Ras85D⁰⁵⁷⁰³ and Df(3R)ΔC40b/Ras85D^e1B transheterozygotes are lethal. Ras85D^e1B/Ras85D⁰⁵⁷⁰³ and Ras85D^e2F/Ras85D⁰⁵⁷⁰³ transheterozygotes are semi-lethal. Ras85D^Sutor-341/Ras85D⁰⁵⁷⁰³ and Ras85D^Sutor-404/Ras85D⁰⁵⁷⁰³ transheterozygotes have low viability. Female transheterozygotes with Ras85D^e2F, Ras85D^Sutor-341 or Ras85D^Sutor-404 lay eggs with a severely disrupted eggshell (patch of chorion instead of dorsal appendages), 57%, 40% and 25% of eggs (respectively) hatch and develop to normal larvae. Unhatched Ras85D⁰⁵⁷⁰³/Ras85D^e2F embryos have normal cuticles, or are missing elements of the head skeleton or have defects in the anterior and posterior termini, holes in the cuticle and disordered posterior segments. Female transheterozygotes with Ras85D^e1B lay eggs with a single dorsal appendage, 84% of eggs hatch and develop to normal larvae. Transheterozygotes with Ras85D^e2F, Ras85D^Sutor-341 or Ras85D^Sutor-404 exhibit slightly disrupted ommatidial array (small number of fused and misplaced ommatidia). Transheterozygotes with Ras85D^e1B exhibit extremely rough eyes (many fused and misplaced ommatidia). (Schnorr and Berg, 1996) female-sterile.
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhancer of
Statement Reference Ras85D⁰⁵⁷⁰³ is an enhancer of visible phenotype of RasGAP1^EP45, Scer\GAL4^hs.2sev (Rorth, 1996)
Phenotype Manifest In
NOT suppressed by
Statement Reference Ras85D⁰⁵⁷⁰³ has phenotype, non-suppressible by Rlb1^hs.PE (Schnorr and Berg, 1996)
Enhancer of
Statement Reference Ras85D⁰⁵⁷⁰³/Ras85D[+] is an enhancer of heart primordium phenotype of sli² (MacMullin and Jacobs, 2006) Ras85D⁰⁵⁷⁰³ is an enhancer of eye phenotype of RasGAP1^EP45, Scer\GAL4^hs.2sev (Rorth, 1996)
Suppressor of
Statement Reference Ras85D⁰⁵⁷⁰³/Ras85D[+] is a suppressor of phenotype of Src42A^Su(phl)1-1 (Zhang et al., 1999)
Additional Comments
Genetic Interactions
Statement Reference Ras85D⁰⁵⁷⁰³/+; sli²/+ embryos produce a mild heart development phenotype. (MacMullin and Jacobs, 2006) Dominantly suppresses the ability of Src42A^Su(phl)1-1 to suppress the lethality of phl¹/Y flies. (Zhang et al., 1999) Expression of Gap1^EP45 in homozygous Ras85D⁰⁵⁷⁰³ flies using Scer\GAL4^hs.2sev produces severely disorganised eyes. (Rorth, 1996) Expression of Rlb1^hs.PE fails to ameliorate the phenotype. (Schnorr and Berg, 1996)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescued by	Ras85D⁰⁵⁷⁰³ is rescued by Ras85D^+t5.5 (Schnorr and Berg, 1996)
Not rescued by	Ras85D⁰⁵⁷⁰³ is not rescued by Ras85D^Δ (Schnorr and Berg, 1996)
Comments	Mutant phenotypes are completely rescued by Ras85D^+t5.5, restoring viability and proper ovarian development. Ras85D^Δ fails to rescue the mutant phenotypes. (Schnorr and Berg, 1996)
Stocks ( 0 )

Notes on Origin
Discoverer	A. Spradling.

Comments
	Precise excision of the P{PZ} insertion reverts the lethal and female sterile phenotypes demonstrating the insertion is responsible for the mutant phenotype. (Schnorr and Berg, 1996) Complements: ms(3)85D⁰³⁵⁶⁵. (BDGP Project Members, 1994-1999)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 8 )
Reported As
Symbol Synonym	85D5703 fs(3)5703 fs(3)05703⁰⁵⁷⁰³ Ras1⁵⁷⁰³ (Zhang et al., 1999, Mantrova and Hsu, 1998, Schnorr and Berg, 1996, Rorth, 1996, Yakoby et al., 2008) Ras85^D05703 (Mahoney et al., 2006) Ras85D⁰⁵⁷⁰³ (Zartman et al., 2008) ras⁵⁷⁰³ (MacMullin and Jacobs, 2006, Settle et al., 2003) Ras^P5703 (Koh et al., 2002)
Name Synonym
Secondary FlyBase IDs

References ( 16 )
Research paper	Yakoby et al., 2008, Dev. Cell 15(5): 725--737 A combinatorial code for pattern formation in Drosophila oogenesis. [FBrf0206199] Zartman et al., 2008, Dev. Biol. 322(2): 289--301 Cad74A is regulated by BR and is required for robust dorsal appendage formation in Drosophila oogenesis. [FBrf0207291] MacMullin and Jacobs, 2006, Dev. Biol. 293(1): 154--164 Slit coordinates cardiac morphogenesis in Drosophila. [FBrf0190142] Mahoney et al., 2006, Genetics 172(4): 2309--2324 Presenilin-based genetic screens in Drosophila melanogaster identify novel Notch pathway modifiers. [FBrf0190744] Ward and Berg, 2005, Mech. Dev. 122(2): 241--255 Juxtaposition between two cell types is necessary for dorsal appendage tube formation. [FBrf0184135] Settle et al., 2003, Oncogene 22(13): 1916--1926 Genetic identification of effectors downstream of Neu (ErbB-2) autophosphorylation sites in a Drosophila model. [FBrf0159278] Koh et al., 2002, J. Neurosci. 22(7): 2496--2504 The Ras1-mitogen-activated protein kinase signal transduction pathway regulates synaptic plasticity through fasciclin II-mediated cell adhesion. [FBrf0151505] Halfar et al., 2001, Development 128(9): 1687--1696 Ras controls growth, survival and differentiation in the Drosophila eye by different thresholds of MAP kinase activity. [FBrf0135730] Schnorr et al., 2001, Genetics 159(2): 609--622 Ras1 interacts with multiple new signaling and cytoskeletal loci in Drosophila eggshell patterning and morphogenesis. [FBrf0139680] Zhang et al., 1999, Genetics 151(2): 697--711 A genetic screen for modifiers of Drosophila Src42A identifies mutations in egfr, rolled and a novel signaling gene. [FBrf0107534] Mantrova and Hsu, 1998, Genes Dev. 12(8): 1166--1175 Down-regulation of transcription factor CF2 by Drosophila Ras/MAP kinase signaling in oogenesis: cytoplasmic retention and degradation. [FBrf0102422] Rorth, 1996, Proc. Natl. Acad. Sci. U.S.A. 93(22): 12418--12422 A modular misexpression screen in Drosophila detecting tissue-specific phenotypes. [FBrf0090768] Schnorr and Berg, 1996, Genetics 144(4): 1545--1557 Differential activity of Ras1 during patterning of the Drosophila dorsoventral axis. [FBrf0091163]
Review	Berg, 2005, Trends Genet. 21(6): 346--355 The Drosophila shell game: patterning genes and morphological change. [FBrf0188357]
Personal communication to FlyBase	Beaton, 1999.12.12, Alleles of the lines in the P-element paper. Alleles of the lines in the P-element paper. [FBrf0125032] BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]

Allele Dmel\Ras85D05703

Allele Dmel\Ras85D⁰⁵⁷⁰³